scholarly journals Determination of phytoplankton composition by microscopy and HPLC-derived pigment analysis in the Sea of Marmara

2020 ◽  
pp. 653
Author(s):  
FUAT DURSUN ◽  
SEYFETTİN TAS ◽  
FATMA BAYRAM PARTAL ◽  
DİLEK EDİGER
Keyword(s):  
Chlorophyll A ◽  
High Performance ◽  
Microscopic Analysis ◽  
Sea Of Marmara ◽  
Phytoplankton Composition ◽  
Pigment Analysis ◽  
Total Phytoplankton ◽  
Research Period ◽  
Taxonomic Evaluation

This study is an integrated overview of pigment and microscopic analysis of phytoplankton composition in the Sea of Marmara. The study was conducted from 27 sampling stations during spring (May 2017 and 2018) and summer (August 2016 and 2018). The phytoplankton community was represented mostly by diatoms and dinoflagellates, as the major groups, and also other phytoflagellate groups as shown by both techniques. Chlorophyll-a, chlorophyll-c1+c2, peridinin, 19’-butanoyloxyfucoxanthin, fucoxanthin, zeaxanthin, alloxanthin, 19’-hexanoyloxyfucoxanthin, diadinoxanthin, chlorophyll-b and ß-carotene concentratios were determined by High Performance Liquid Chromatography (HPLC). A total of 124 eukaryotic taxa belonging to eight algal classes were found through microscopic analysis. 60 taxa of them were dinoflagellates, 52 taxa were diatoms and 12 taxa were other phytoflagellates. The number of diatoms and dinoflagellates, as the major groups, accounted for 90.4% of the total phytoplankton species. Chlorophyll-a, fucoxanthin and peridinin concentrations varied between 0.03-7.20, 0.01-5.23 and 0.01-2.14 μg L-1, throughout the research period, respectively. The highest chlorophyll-a values were measured at stations MD26 (7.20 µg L-1) and MD22 (6.61 µg L-1) in May 2018, which were located at Gemlik and Bandirma bays. There was a significant correlation (r:0.87, p<0.001 n:54) between HPLC determined fucoxanthin concentrations and diatom abundances in August 2016 and 2018. Also, higher chlorophyll-c1+c2 concentrations revealed consistency with high diatom abundances and fucoxanthin concentrations in August 2016 and 2018. This result confirmed that diatoms are the most important carrier of fucoxanthin and it can be used for taxonomic evaluation of diatom community of the Sea of Marmara.

Characterization of the photoautotrophic algal and bacterial communities in a large, shallow, subtropical lake using HPLC-PDA based pigment analysis

1998 ◽  
Vol 55 (1) ◽  
pp. 206-219 ◽  
Author(s):  
Alan D Steinman ◽  
Karl E Havens ◽  
J William Louda ◽  
Nancy M Winfree ◽  
Earl W Baker
Keyword(s):  
Water Column ◽  
Chlorophyll A ◽  
High Performance ◽  
Principal Component ◽  
Open Water ◽  
High Performance Liquid Chromatographic ◽  
Lake Okeechobee ◽  
Pheophytin A ◽  
Pigment Analysis ◽  
Liquid Chromatographic

Pigment abundances of the oxygenic and anoxygenic photoautotrophic communities from sediments and the water column in Lake Okeechobee, Florida, were estimated using reverse-phase high-performance liquid chromatographic (RP-HPLC) and photodiode array (PDA) UV/VIS (350-800 nm) spectrophotometric analyses. Thirty lipophilic pigments were identified and measured in the samples, with the most abundant overall (sediment and open-water samples combined) being chlorophyll a (38.1%), fucoxanthin (12.6%), pheophytin a (7.6%), zeaxanthin (6.6%), and pyropheophytin a (3.6%). Relative abundance of chlorophyll a was greater in the water column than in the sediments (58.3 versus 24.3% of all pigments) whereas pheophytin a comprised 9.1% of the total pigments in the sediments but only 3.7% of the total pigments in the water column. Principal component analysis (PCA) separated the sediment samples from those collected in the water column; this discrimination appears to be a function of pigment integrity in that sediment assemblages had much greater relative abundances of degraded pigments. Different regions of the lake were weakly separated by PCA based on pigments. The relatively weak degree of separation may reflect the overwhelming abundance of chlorophyll a at all sites. Overall, the pigment assemblage in Lake Okeechobee suggests cyanobacteria-diatom dominance. Out of 65 sampling events, pigments from anoxygenic photoautotrophs (e.g., bacteriochlorophylls) were detected 17 times but accounted for >20% of total chlorophyll only five times. Bacteriochlorophylls were observed only in the sediments and were most abundant during June and September, when winds were calm and temperatures warm, and at relatively shallow sites.

scholarly journals Assessment of phytoplankton group composition in the Golden Horn Estuary (Sea of Marmara, Turkey) determined with pigments measured by HPLC-CHEMTAX analyses and microscopy

2021 ◽  
pp. 1-17
Author(s):  
Fuat Dursun ◽  
Seyfettin Tas ◽  
Dilek Ediger
Keyword(s):  
Surface Water ◽  
High Performance ◽  
Group Composition ◽  
Microscopic Analysis ◽  
Sea Of Marmara ◽  
Relative Contribution ◽  
Golden Horn ◽  
Eukaryotic Phytoplankton ◽  
Average Contribution ◽  
Golden Horn Estuary

Abstract Phtytoplankton group composition determined by microscopy was compared with high performance liquid chromatography (HPLC) derived from pigment signatures in surface water samples taken bi-weekly and monthly between October 2018 and September 2019 in the Golden Horn Estuary (Sea of Marmara). A total of 80 eukaryotic phytoplankton taxa belonging to eight algal classes were identified in surface water during the study period. Forty-three taxa (54%) were diatoms, 29 taxa (36%) were dinoflagellates and eight taxa (10%) were other phytoflagellates. The average contribution of diatoms to total phytoplankton abundance decreased considerably (41 to 25%), while the average contribution of dinoflagellates and other phytoflagellates increased markedly (59 to 75%) from the lower to the middle estuary. Chlorophyll-a and seven other group-specific pigments, including fucoxanthin, peridinin, chlorophyll-c1 + c2, alloxanthin, 19′-hexanoyloxyfucoxanthin, 19′-butanoyloxyfucoxanthin and divinyl chlorophyll-a were identified in the study area. The relative contribution of the major phytoplankton groups to chlorophyll-a was estimated on three different initial ratio matrices by CHEMTAX. The results obtained were compared with those from microscopic examination. It was concluded that the CHEMTAX method was not accurate enough to characterize the phytoplankton community in the Golden Horn Estuary ecosystem and microscopic analysis was essential to determine the major contributing species to chlorophyll-a.

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