scholarly journals Molecular Characterization by of Bacterial Isolates and Physicochemical Assessment of Well Water Samples from Hostels at Osekita, Iworoko-Ekiti, Ekiti State

2018 ◽  
Vol 6 (1) ◽  
pp. 22-32
Author(s):  
Odeyemi A. T. ◽  
Ayantola K. J. ◽  
Peter S.
Author(s):  
R. R. Nrior ◽  
M. Okpokiri ◽  
N. P. Akani

Antibiotic resistance has become a great global problem. Thus, it has emerged as a public health challenge. The antibiotic susceptibility pattern of bacteria in well water was characterized with a view of determining the level of resistance in the environment. Fifty well water samples were collected from ten different points in Ula-Ubie community, Ahoda, Rivers State for a period of five months. Standard microbiological methods were used to analyse the population and types of bacteria in the water while methods recommended by the American Public Health Association (APHA) was used to determine the physicochemical parameters of the samples. The antibiotic susceptibility profile of the bacterial isolates was carried out using the disc diffusion methods. The total heterotrophic bacteria of the water samples ranged from 0.93±0.46 to 2.02±1.06 log10 CFU/ml. The coliform counts ranged from 0.45±0.42 - 2.55±2.33 log10 CFU/ml, respectively. Despite the variations in the counts of the different bacterial population, there was no significant differences (P > 0.05) in the different well water samples. The physicochemical parameters except the pH were all within the permissible limits. Klebsiella spp, Pseudomonas spp, Serratia spp and Enterobacter spp were identified in the well water. The pH of the water stations ranged from 4.66 to 5.80. The temperature ranged from 24.0 to 24.7. The electrical conductivity, salinity, dissolved oxygen, total hardness, alkalinity, total suspended solids, biochemical oxygen demand, nitrate, chloride, calcium and magnesium ranged from 22.9 – 219, 0.03-0.13, 4.50-4.90, 5.00-22.0, ˂0.01-3.00, ˂0.01, 49.6-84.5, 1.00-17.4, 3.00-24.5, 4.25-12.9 and 0,722-1.55 respectively. The antibiotic susceptibility profile showed that all the isolates were resistant to ceftazidime and augmentin, whereas Enterobacter spp were the most resistant bacteria amongst other bacterial genera to the antibiotics. Meanwhile there is an existence of multi-drug resistance. Thus, the wells could be considered not potable due to the presence of these bacterial isolates and the level of antibiotic resistant. Proper sanitation and cleanliness of well should be encouraged.


2020 ◽  
Vol 2 (2) ◽  
pp. 46-52
Author(s):  
Hendri Iyabu ◽  
Anita Muhammad ◽  
Jafar La Kilo ◽  
Akram La Kilo

This study aims to determine the iron content of water wells in Dulalowo and Heledulaa, Gorontalo City. Ten well water samples were taken randomly from five different wells in each district. Water samples at each well consist of top, middle, and bottom water. Determination of iron level using Atomic Absorption Spectrophotometer (AAS) at a wavelength of 248.33 nm. The results showed that the iron concentration of well water in Dulalowo was 0.030 (D1), 0.265 (D2), 0.224 (D3), 0.158 (D4), 0.149 ppm (D5). Meanwhile, iron levels in Heledulaa were 0.100 (H1), 0.039 (H2), 0.159 (H3), 0.198 (H4), and 0.235 ppm (H5). These results prove that the Fe content in the well water in the two districts is still fulfil the drinking water standard which is a maximum of 0.3 mg/L iron.


Water ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 392 ◽  
Author(s):  
Sabir Nurtazin ◽  
Steven Pueppke ◽  
Temirkhan Ospan ◽  
Azamat Mukhitdinov ◽  
Timur Elebessov

The thinly populated Balkhash District of Kazakhstan’s Almaty Region lies in the lower reaches of the Ili-Balkhash basin, which is shared by China and Kazakhstan. The district is arid and heavily dependent on inflows of surface water, which are threatened by the effects of upstream population growth, economic development, and climate change. The quality of drinking water from centralized water systems and tube wells in nine villages of the district was analyzed, and the organoleptic properties of water from these sources was also assessed by an expert and via surveys of local residents. Although most samples met governmental standards for the absence of chemical impurities, high concentrations of mineralization, chlorides, boron, iron, and/or uranium were present in some well water samples. Levels of these pollutants were as much as 4-fold higher than governmental maxima and as much as 16-fold higher than concentrations reported previously in surface water. All centralized water samples met standards for absence of microbial contamination, but total microbial counts in some well water samples exceeded standards. Organoleptic standards were met by all the water from five villages, but centralized water from one village and well water from four villages failed to meet standards based on expert judgment. Residents were, for the most part, more satisfied with centralized rather than well water, but there was no obvious relationship between the failure of water to meet standards and the locations or populations of the settlements. This is the first comprehensive assessment of groundwater used for drinking in the lower Ili-Balkhash basin, and although it relies on a limited number of samples, it nevertheless provides evidence of potentially serious groundwater contamination in the Balkhash District. It is thus imperative that additional and more detailed studies be undertaken.


2020 ◽  
Vol 9 (1) ◽  
pp. 12-14
Author(s):  
Md Aftab Uddin ◽  
Mst Aysha Siddiqua ◽  
Mst Sadia Ahmed

Commercial drinking water may serve as potential threat to public health if these items are contaminated with a number of pathogenic microorganisms due to faulty manufacturing process. Present study attempted to isolate and quantify the microorganisms from various jar and bottle water samples collected from various areas of Dhaka city. Antibiotic susceptibility pattern of suspected bacterial isolates were also determined in this study. Out of the eighteen samples studied, ten were jar water samples and eight were bottled water samples. The range of total viable bacterial count (TVBC) in these samples ranged from 102 to 105 cfu/ml. Specific pathogens such as, Salmonella spp., Shigella spp., Vibrio spp. and fecal coliforms could not be found in these samples. However coliforms could be detected in 10 samples. The antibiogram study showed that all Escherichia coli and Klebsiella spp. isolates found from these samples were sensitive against gentamicin (10 μg) and azithromycin (30 μg). Variable antibiotic resistance among these bacterial isolates was detected against cefotaxime (30 μg), streptomycin (10 μg) and erythromycin (15 μg). Stamford Journal of Microbiology, Vol.9(1) 2019: 12-14


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