Prevalence and Antibiogram of Gram-negative Bacteria Isolated from Well Water in Ula-Ubie Community, Ahoada West, Nigeria

Antibiotic resistance has become a great global problem. Thus, it has emerged as a public health challenge. The antibiotic susceptibility pattern of bacteria in well water was characterized with a view of determining the level of resistance in the environment. Fifty well water samples were collected from ten different points in Ula-Ubie community, Ahoda, Rivers State for a period of five months. Standard microbiological methods were used to analyse the population and types of bacteria in the water while methods recommended by the American Public Health Association (APHA) was used to determine the physicochemical parameters of the samples. The antibiotic susceptibility profile of the bacterial isolates was carried out using the disc diffusion methods. The total heterotrophic bacteria of the water samples ranged from 0.93±0.46 to 2.02±1.06 log10 CFU/ml. The coliform counts ranged from 0.45±0.42 - 2.55±2.33 log10 CFU/ml, respectively. Despite the variations in the counts of the different bacterial population, there was no significant differences (P > 0.05) in the different well water samples. The physicochemical parameters except the pH were all within the permissible limits. Klebsiella spp, Pseudomonas spp, Serratia spp and Enterobacter spp were identified in the well water. The pH of the water stations ranged from 4.66 to 5.80. The temperature ranged from 24.0 to 24.7. The electrical conductivity, salinity, dissolved oxygen, total hardness, alkalinity, total suspended solids, biochemical oxygen demand, nitrate, chloride, calcium and magnesium ranged from 22.9 – 219, 0.03-0.13, 4.50-4.90, 5.00-22.0, ˂0.01-3.00, ˂0.01, 49.6-84.5, 1.00-17.4, 3.00-24.5, 4.25-12.9 and 0,722-1.55 respectively. The antibiotic susceptibility profile showed that all the isolates were resistant to ceftazidime and augmentin, whereas Enterobacter spp were the most resistant bacteria amongst other bacterial genera to the antibiotics. Meanwhile there is an existence of multi-drug resistance. Thus, the wells could be considered not potable due to the presence of these bacterial isolates and the level of antibiotic resistant. Proper sanitation and cleanliness of well should be encouraged.

Download Full-text

Antibiotic susceptibility pattern of Klebsiella pneumoniae and Pseudomonas aeruginosa isolated from some drinking wells in Ondo town southwest Nigeria

Journal of Applied Sciences and Environmental Management ◽

10.4314/jasem.v25i1.8 ◽

2021 ◽

Vol 25 (1) ◽

pp. 59-63

Author(s):

O. Aromolaran ◽

O.A. Amodu

Keyword(s):

Pseudomonas Aeruginosa ◽

Klebsiella Pneumoniae ◽

Water Samples ◽

Antibiotic Susceptibility ◽

Diffusion Method ◽

Resistant Bacteria ◽

Well Water ◽

Susceptibility Pattern ◽

Antibiotic Susceptibility Pattern ◽

Southwest Nigeria

Antibiotic-resistant bacteria (ARB) strains have become a global health threat. This study aimed to determine the antibiotic susceptibility pattern of Klebsiella pneumoniae and Pseudomonas aeruginosa isolated from wells in Ondo town, Southwest Nigeria. Twenty-eight well water samples were analyzed for the presence of K. pneumoniae and P. aeruginosa by standard pour plate technique. The bacterial isolates were tested against eight commonly use antibiotics using Kirby Bauer disc diffusion method. The percentage occurrence of K. pneumoniae and P. aeruginosa in the well water samples were 17.86% and 21.43%, respectively. Two multi-drug resistant strains of K. pneumoniae were isolated, which were resistant to at least three classes of antibiotics. Fifty percent of the P. aeruginosa isolates were resistant to caftazidime, cefuroxime, nitrofurantoin, and ampicillin. None of the isolates was fully susceptible to cefuroxime, but have all showed resistance to β-lactam (ceftazidime, cefuroxime augmentin, and ampicillin) antibiotics. Cefuroxime may not be effective an effective drug in the treatment of K. pneumoniae and P. aeruginosa implicated infections in these communities in Ondo. Also, the over-use of antibiotics should be discouraged in order to curtail the menace of antibiotic resistance Keywords: Well water, Antibiotics, Klebsiella pneumoniae, Pseudomonas aeruginosa

Download Full-text

Antibiotic Resistance Pattern of Bacteria Isolated from Biofilms in Water from Groundwater Sources in Ado-Ekiti, Nigeria

South Asian Journal of Research in Microbiology ◽

10.9734/sajrm/2019/v3i430093 ◽

2019 ◽

pp. 1-9 ◽

Cited By ~ 1

Author(s):

A. O. Olalemi ◽

F. T. Akinruli ◽

V. O. Oluwasusi

Keyword(s):

Antibiotic Resistance ◽

Water Samples ◽

Bacterial Count ◽

Shigella Dysenteriae ◽

Resistant Bacteria ◽

Microbiological Analysis ◽

Bacterial Isolates ◽

Streptococcus Faecalis ◽

Antibiotic Resistant ◽

Microbiological Methods

This study investigated the pattern of occurrence of antibiotic resistant bacteria in biofilms in water from groundwater sources in Ado-Ekiti, Nigeria. Water samples were collected from boreholes and wells within Ado-Ekiti metropolis over a period of 4 months (n = 100), and biofilm samples were taken at interval of seven days within the period of storage and subjected to microbiological analysis until the total bacterial counts were significant. Enumeration of bacteria in biofilms and antibiotic sensitivity of the bacterial isolates were carried out using standard microbiological methods and multiple antibiotic resistant indexes of the bacterial isolates were calculated. Results showed that a total of 202 bacterial isolates were obtained from the biofilms of the water samples and this include Streptococcus faecalis, Escherichia coli, Enterobacter aerogenes, Pseudomonas aeruginosa, Proteus vulgaris, Staphylococcus aureus, Salmonella typhi and Shigella dysenteriae. Of all the bacterial isolates, Streptococcus faecalis had the highest frequency of occurrence (90 %). The bacterial isolates from the biofilms in water from borehole had the highest bacterial count (1.11 × 104 cfu/ml) and were more resistant to antibiotics, whereas those from well had the least bacterial count (0.78 × 104 cfu/ml) and were less resistant to antibiotics. A total of 106 (52.5%) bacterial isolates displayed multiple antibiotic resistance (MAR) with indexes greater than 0.2. The findings from this study suggest high prevalence of MAR indexes indicating high source of contamination in areas where antibiotics are used in Ado-Ekiti. Water from the groundwater sources should be treated at point of use and should not be stored for too long before use to prevent the development of biofilms that may be of great significance to human health.

Download Full-text

Prevalence of Multidrug-Resistant Bacteria Isolates in Waste Water from Different Hospital Environment in Umuahia, Nigeria

International Journal of Pharmaceutical Sciences Review and Research ◽

10.47583/ijpsrr.2021.v69i02.003 ◽

2021 ◽

Vol 69 (2) ◽

Author(s):

Uzoije U.N ◽

Iroha I.R ◽

Moses I.B ◽

Ukwa B.N ◽

Onuorah A.L ◽

...

Keyword(s):

Public Health ◽

Waste Water ◽

Water Samples ◽

Multidrug Resistant ◽

Hospital Environment ◽

Health Concern ◽

Diffusion Method ◽

Resistant Bacteria ◽

Bacterial Isolates ◽

Hospital Waste

The discharge of untreated hospital waste water into the environment is a major public health concern as this could result in the environmental spread of multidrug-resistant (MDR) bacteria. The dissemination of such MDR bacteria in waste water remains unexamined in most geographical area. This study assessed the prevalence of multidrug-resistant bacterial isolates in waste water from hospital environment in Umuahia, Nigeria. Exactly 200 waste water samples were collected from various diagnostic laboratory units of Michael Okpara University of Agriculture, Umudike (MOUAU) Health Centers and Federal Medical Center (FMC), Umuahia in 250 ml screw-capped, heat-sterilized bottles. Water samples were analyzed using standard microbiological techniques. Bacterial isolates from water samples were identified with API-20E test kit. Antibiotic susceptibility test was done using Kirby–Bauer disc diffusion method. Multiple antibiotic resistance index (MARI) of isolated bacteria was determined using standard formulae. A total of 147(73.5 %) bacterial species such as S. aureus, Shigella spp, E. coli, Enterobacter spp, Proteus mirabilis, and Arizona spp were identified from the waste water samples. Bacterial isolates exhibited resistance to tetracycline, trimethoprim-sulfamethoxazole, clindamycin, nalixidic acid, vancomycin, and chloramphenicol but very susceptible to imipenem. All isolates were multidrug-resistant with MARI values ranging from 0.5 - 0.8. This study revealed the presence of MDR bacteria in hospital waste water samples in Umuahia, Abia state, Nigeria. The threat and risk of exposure to such MDR bacteria is of public health significance and raises concern over poor management and disposal of hospital waste water or effluents.

Download Full-text

Isolation and Characterization of Pb Resistant Bacteria from Cilalay Lake, Indonesia

Aceh International Journal of Science and Technology ◽

10.13170/aijst.4.3.3016 ◽

2015 ◽

Vol 4 (3) ◽

Author(s):

Kesi Kurnia ◽

Nina Hermayani Sadi ◽

Syafitri Jumianto

Keyword(s):

Heterotrophic Bacteria ◽

Water Environment ◽

Resistant Bacteria ◽

Bacterial Isolates ◽

Gram Negative ◽

Isolation And Characterization ◽

Agar Media ◽

Physical And Chemical ◽

Standard Disk

Pollution of water environment with heavy metals is becoming one of the most severe environmental and human health hazards. Lead (Pb) is a major pollutant and highly toxic to human, animals, plants, and microbes. Toxic metals are difficult to remove from the environment, since they cannot be chemically or biologically degraded and are ultimately indestructible. Biological approaches based on metal-resistant microorganisms have received a great deal of attention as alternative remediation processes. This study aim to isolate and characterize Pb resistant of heterotrophic bacteria in Cilalay Lake, West Java, Indonesia. The water samples were collected along three points around Cilalay Lake. Water physical and chemical determination was performed using the Water Quality Checker. The bacterial isolates were screened on Triptone Glucose Yeast (TGY) agar plates. Afterwards selected isolates were grown on Nutrient Agar media 50% with supplemented Pb 100 ppm by the standard disk. Population of resistant bacteria was counted. The result from metal resistant bacteria indicated that all isolates were resistant. The most abundant type of resistant bacteria to lead was Gram negative more than Gram positive. Identified have metal resistant bacteria could be useful for the bioremediation of heavy metal contaminated sewage and waste water

Download Full-text

Bacterial Isolates and Their Antibiograms of Burn Wound Infections in Burns Specialist Hospital in Baghdad

Baghdad Science Journal ◽

10.21123/bsj.10.2.331-340 ◽

2013 ◽

Vol 10 (2) ◽

pp. 331-340 ◽

Cited By ~ 1

Author(s):

Baghdad Science Journal

Keyword(s):

Generation Cephalosporin ◽

Multi Drug Resistance ◽

Resistant Bacteria ◽

Bacterial Isolates ◽

Burn Wound ◽

Hospital Policy ◽

Acinetobacter Baumanii ◽

Fifth Generation ◽

Drug Resistant Bacteria ◽

Providencia Stuartii

A total of 54 out of 67 (80.59%) of burn wound swab showed growth of one, or two, or three bacterial pathogens. Pseudomonas aeruginosa was the commonest pathogen, isolated in 48.14% of swab samples, followed by Klebsiella pneumoniae (31.48%), Staphylococcus aureus (27.77%), Acinetobacter baumanii (14.81%), Escherichia coli (7.40%), and Citrobacter freundii, Providencia stuartii, Enterobacter cloacae, with 1.85% isolation percentage for each. All bacterial isolates were tested against 19 antibiotics, and showed multi-drug resistance to 10 antibiotics, or more. The most effective antibiotics were the fifth-generation cephalosporin, ceftobiprole, and and antibiotic combinations, as Ceftazidime / clavulanic acid, and Cefoperazone /sulbactam, and newer generation fluoroquinolone, levofloxacin, and gemifloxacin, which are attractive candidates to be the basic antibiotics in establishment of new hospital policy in Iraq for treatment of burn wound infection of multi-drug resistant bacteria

Download Full-text

Multidrug Resistant and Extensively Drug Resistant Bacteria: A Study

Journal of Pathogens ◽

10.1155/2016/4065603 ◽

2016 ◽

Vol 2016 ◽

pp. 1-5 ◽

Cited By ~ 46

Author(s):

Silpi Basak ◽

Priyanka Singh ◽

Monali Rajurkar

Keyword(s):

Antimicrobial Resistance ◽

Antibiotic Susceptibility ◽

Multidrug Resistant ◽

Resistant Bacteria ◽

Close Monitoring ◽

Care Hospital ◽

Drug Resistant ◽

Bacterial Isolates ◽

Bacterial Strains ◽

Extensively Drug Resistant

Background and Objective. Antimicrobial resistance is now a major challenge to clinicians for treating patients. Hence, this short term study was undertaken to detect the incidence of multidrug-resistant (MDR), extensively drug-resistant (XDR), and pandrug-resistant (PDR) bacterial isolates in a tertiary care hospital.Material and Methods. The clinical samples were cultured and bacterial strains were identified in the department of microbiology. The antibiotic susceptibility profile of different bacterial isolates was studied to detect MDR, XDR, and PDR bacteria.Results. The antibiotic susceptibility profile of 1060 bacterial strains was studied. 393 (37.1%) bacterial strains were MDR, 146 (13.8%) strains were XDR, and no PDR was isolated. All (100%) Gram negative bacterial strains were sensitive to colistin whereas all (100%) Gram positive bacterial strains were sensitive to vancomycin.Conclusion. Close monitoring of MDR, XDR, or even PDR must be done by all clinical microbiology laboratories to implement effective measures to reduce the menace of antimicrobial resistance.

Download Full-text

Analysis of the Physicochemical Parameters and Microbiological Quality of Water Samples Obtained from Mbiaso River and Enang Stream in Ikot Ekpene Metropolis

Journal of Advances in Microbiology ◽

10.9734/jamb/2021/v21i630356 ◽

2021 ◽

pp. 18-27

Author(s):

Ekanem, Jonathan Okokon ◽

Akpan, Nyaknoabasi Ime ◽

Zorbari, Glory Msubaritoma

Keyword(s):

Water Samples ◽

Physicochemical Parameters ◽

Heterotrophic Bacteria ◽

Microbiological Quality ◽

Titratable Acidity ◽

Total Coliform ◽

Coliform Count ◽

Quality Of Water ◽

Sampling Points ◽

Who Standards

The physicochemical, microbiological and heavy metal studies of water samples obtained from Mbiaso River and Enang Stream were investigated. Water samples were collected from two locations with two sampling points from each location along the course of the water body based on their use by the community and analysed using standard procedures. The results obtained were compared with WHO standards for drinking and recreational water. Aside titratable acidity, titratable alkalinity and BOD, all other physicochemical parameters were within the permissible standards. All the heavy metals concentrations were above the permissible limits except for Lead (Pb) that ranged between 0.01mg/l to 0.05 mg/l for all the sampling points. All the water samples were found to harbor coliforms organisms in numbers greater than the required WHO standards for water except for Enang river that lacked the presence of Vibrio spp. The total viable counts for all the water samples were generally high exceeding the standard limit of 1.0 x 102 cfu/ml for water. The total heterotrophic bacteria count, total coliform count, faecal coliform count and total fungal count ranged between 1.9x104 - 52x104 cfu/ml, 2.3x104 -38 x104 cfu/ml, 1.7x104 - 30x104 cfu/ml and 2.5x104 - 5.3x104 cfu/ml respectively. The total salmonella-shigella counts and total staphylococcus counts were in the range of 0.2 x102-0.4 x102 cfu/ml and 0.3 x102 -0.6 x104 cfu/ml respectively. From the study, it is suggested that some regulations measures be put in place to control pollution of the water while the water be properly treated before use for domestic, potable or recreational purposes.

Download Full-text

Antibiotic susceptibility profile of bacterial pathogens isolated from febrile children under 5 years of age in Nanoro, Burkina Faso.

10.21203/rs.3.rs-42554/v1 ◽

2020 ◽

Author(s):

Massa dit Achille BONKO ◽

Marc Christian Tahita ◽

Francois Kiemde ◽

Palpouguini Lompo ◽

Sibidou Yougbaré ◽

...

Keyword(s):

Drug Resistance ◽

Burkina Faso ◽

Antibiotic Susceptibility ◽

Bacterial Species ◽

Penicillin G ◽

Multi Drug Resistance ◽

Bacterial Isolates ◽

Study Region ◽

Clinical Specimens ◽

Children Under 5

Abstract Background: The curative power of antimicrobials is severely threatened due to emerging resistance to first-line antibiotics worldwide. With a limited reserve of antibiotics, increasing antimicrobial resistance has become a global concern, but there is a paucity of such data in Burkina Faso, and the West African region in general. Therefore, this study aims to determine the antibiotic susceptibility profile of bacterial species isolated from febrile children under 5 years of age in Nanoro (Burkina Faso). Methods: Clinical specimens (blood, stool, and urine) were collected from 1099 febrile children attending the peripheral health facilities and the referral hospital in Nanoro. Bacterial isolates from these clinical specimens were assessed for their susceptibility against commonly used antibiotics by standard disc diffusion procedure and minimal inhibitory concentration method (when appropriate). Results: In total, 141 bacterial strains were recovered from 127 febrile children of which 65 strains were isolated from blood, 65 from the stool, and 11 from urine. Predominant bacterial isolates were Salmonella species (56.7%; 80/141) followed by Escherichia coli (33.3%; 47/141). Antibiotic susceptibility testing revealed Salmonella species were highly resistant to ampicillin (70%; 56/80), trimethoprim-sulfamethoxazole (65%; 52/80), and chloramphenicol (63.8%; 51/80). E. coli isolates were highly resistant to trimethoprim-sulfamethoxazole (100%), ampicillin (100%), ciprofloxacin (71.4%; 10/14), amoxicillin-clavulanate (64.3%; 9/14), ceftriaxone (64.3%; 9/14), and gentamycin (50%; 7/14). Moreover, 7 out of 14 E. coli isolates were producers of the ß-lactamase enzyme, suggesting multi-drug resistance against b-lactam as well as non-b-lactam antibiotics. S. pneumoniae isolates were fully resistant to tetracycline and 50% to penicillin G. Multi-drug resistance was observed in 54.6% (59/108) of the isolates of which 56 (54.9%) were Gram-negative bacteria and 3 (50.0%) Gram-positive bacteria.Conclusions: The antibiotic susceptibility profiling showed an alarming high resistance to commonly used antibiotics to treat bacterial infections in the study region. The work prompts the need to expand antibiotic resistance surveillance studies in Burkina Faso, and probably the whole region (West Africa). Moreover, it implies the need of a revision of the antibiotic-treatment guidelines by the Ministry of Health in Burkina Faso to avoid further development of resistance.

Download Full-text

Assessment of the Bacteriological Profile and Antibiotic Susceptibility Patterns of Wastewater in Health Facilities of Ethiopia

International Journal of Microbiology ◽

10.1155/2021/9969479 ◽

2021 ◽

Vol 2021 ◽

pp. 1-6

Author(s):

Belayneh Regasa Dadi ◽

Eyayu Girma ◽

Mheret Tesfaye ◽

Mohamed Seid

Keyword(s):

Health Facility ◽

Antibiotic Susceptibility ◽

Multidrug Resistant ◽

Health Facilities ◽

Resistant Bacteria ◽

Coagulase Negative Staphylococcus ◽

Bacterial Isolates ◽

Bacteriological Profile ◽

Antibiotic Susceptibility Patterns ◽

Susceptibility Patterns

Background. Antimicrobials used for the treatment and prevention of bacterial infections are mainly released nonmetabolized into the aquatic environment via wastewater. Sometimes, unused therapeutic drugs are released down the drains that could act as selective pressure for the development of resistance. The aim of this study was to assess the bacteriological profile of wastewater in health facilities and determine antibiotic susceptibility patterns of bacterial isolates. Methods. A cross-sectional study was conducted from October 1 to December 26, 2020, in health facility wastewater. A total of 128 samples were collected from health facilities for bacteriological analysis and antimicrobial susceptibility testing. Result. A total of 128 samples were processed, and 81 bacterial isolates were recovered. The most common bacterial isolates were S. aureus (16/81 (19.8%)) followed by Klebsiella spp. (15/81 (18.5%)), E. coli (13/81 (16%)), P. aeruginosa (10/81 (12.3%)), Enterobacter spp. (8/81 (9.9%)), Citrobacter spp. (7/81 (8.6%)), coagulase-negative Staphylococcus (5/81 (6.2%)), Salmonella spp. (5/81 (6.2%)), and Shigella spp. (2/81 (2.5%)). A majority of isolates were resistant to ampicillin (62/81 (76.5%)). Only few isolates were resistant to ciprofloxacin (11/81 (13.6%)), chloramphenicol (13/81 (16%)), and kanamycin (8/54 (14.8%)). A majority of bacterial isolates (57/81 (70.4%)) were multidrug resistant (MDR). Conclusion. Wastewater from the health facilities contains antibiotic-resistant including multidrug-resistant bacteria. Therefore, health facility wastewater should be treated by appropriate wastewater treatment before being released into the environment.

Download Full-text

Isolation and Molecular Characterisation of Vancomycin Resistant Bacteria Isolated from Selected Well Water in Ula-Ubie Community, Ahoada, Rivers State

Journal of Advances in Microbiology ◽

10.9734/jamb/2020/v20i230221 ◽

2020 ◽

pp. 70-77

Author(s):

N. P. Akani ◽

M. Okpokiri ◽

R. R. Nrior

Keyword(s):

Screening Method ◽

Brain Heart Infusion ◽

Molecular Methods ◽

Nutrient Agar ◽

Health Concern ◽

Resistant Bacteria ◽

Well Water ◽

Bacterial Isolates ◽

Vancomycin Resistant ◽

Rivers State

The resistance of bacteria to vancomycin has made the treatment of some infections more difficult to handle. The aim of this study was to isolate and molecularly characterize vancomycin resistant bacteria in selected well water with a view of determining the level of resistance in the environment. fifty (50) well water samples were collected from ten different stations in Ula-Ubie community, Ahoada, Rivers State for a period of six months (January 2019 to June, 2019). Bacteria diversity was analysed using standard microbiological techniques. In this technique, aliquots of 10-1, 10-2 and 10-3 dilutions were seeded into prepared Nutrient agar and Brain Heart infusion agar (BHI). Microbial loads were enumerated and distinct bacterial colonies were picked and inoculated on freshly prepared nutrient agar. Purification of isolates was done by streaking on freshly prepared nutrient agar plates until isolates were void of mixed culture. The isolates were further characterized using biochemical and molecular methods and preserved in bijou bottles containing 5 ml of 10% (v/v) glycerol. Isolates from the glycerol were subcultured on fresh nutrient agar plates before each use. The vancomycin resistant bacteria were screened using the culture-based screening method. In this method, sterile nutrient medium was supplemented with different concentrations of the vancomycin antibiotics. Inoculation of bacterial isolates on the vancomycin supplemented agar followed before plates were incubated. The ensuing bacterial isolates were characterized using biochemical and molecular methods. Ninetine (19) bacteria isolates were screened for vancomycin and of this numbers, six (6) were positive for vancomycin resistance. The agar rose gel electrophoresis showed the presence of vanA and vanB gene which could be responsible for the resistance to the vancomycin observed in most isolates. The presence of vancomycin resistant genes in these isolates is of public health concern due to the transfer of resistance to other isolates. Standard hygienic practice is therefore recommended in the use of well water.

Download Full-text