Identification of Enterocin NKR-5-3C, a Novel Class IIa Bacteriocin Produced by a Multiple Bacteriocin Producer, Enterococcus faecium NKR-5-3

2012 ◽  
Vol 76 (6) ◽  
pp. 1245-1247 ◽  
Author(s):  
Kohei HIMENO ◽  
Koji FUJITA ◽  
Takeshi ZENDO ◽  
Pongtep WILAIPUN ◽  
Naoki ISHIBASHI ◽  
...  
Keyword(s):  
Enterococcus Faecium ◽  
Bacteriocin Producer

scholarly journals Draft Genome Sequence of Probiotic Enterococcus faecium Strain L-3

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Alena Karaseva ◽  
Anna Tsapieva ◽  
Justin Pachebat ◽  
Alexander Suvorov
Keyword(s):  
Genome Sequence ◽  
Russian Federation ◽  
Enterococcus Faecium ◽  
Draft Genome ◽  
Bacteriocin Production ◽  
Draft Genome Sequence ◽  
Bacteriocin Producer ◽  
Probiotic Preparation ◽  
The Russian Federation ◽  
Faecium Strain

We report here the draft genome sequence of the bacteriocin producer Enterococcus faecium strain L-3, isolated from a probiotic preparation, Laminolact, which is widely used in the Russian Federation. The draft genome sequence is composed of 74 contigs for a total of 2,643,001 bp, with 2,646 coding genes. Five clusters for bacteriocin production were found.

Molecular analysis of the replication region of the pCIZ2 plasmid from the multiple bacteriocin producer strain Enterococcus faecium L50

Plasmid ◽  
2008 ◽  
Vol 60 (3) ◽  
pp. 181-189 ◽  
Author(s):  
R. Criado ◽  
J. Gutiérrez ◽  
A. Budin-Verneuil ◽  
P.E. Hernández ◽  
A. Hartke ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Enterococcus Faecium ◽  
Producer Strain ◽  
Bacteriocin Producer ◽  
Replication Region

Processing and secretion of non-cognate bacteriocins by EnkT, an ABC transporter from a multiple-bacteriocin producer, Enterococcus faecium NKR-5-3

2020 ◽  
Vol 130 (6) ◽  
pp. 596-603
Author(s):  
Hirotoshi Sushida ◽  
Miyuki Sakei ◽  
Rodney H. Perez ◽  
Naoki Ishibashi ◽  
Takeshi Zendo ◽  
...  
Keyword(s):  
Abc Transporter ◽  
Enterococcus Faecium ◽  
Bacteriocin Producer

scholarly journals Complete Sequence of the Enterocin Q-Encoding Plasmid pCIZ2 from the Multiple Bacteriocin Producer Enterococcus faecium L50 and Genetic Characterization of Enterocin Q Production and Immunity

2006 ◽  
Vol 72 (10) ◽  
pp. 6653-6666 ◽  
Author(s):  
Raquel Criado ◽  
Dzung B. Diep ◽  
Ågot Aakra ◽  
Jorge Gutiérrez ◽  
Ingolf F. Nes ◽  
...  
Keyword(s):  
Enterococcus Faecium ◽  
Complete Sequence ◽  
Open Reading Frames ◽  
Genetic Determinants ◽  
Significant Similarity ◽  
Minimum Requirement ◽  
Initiator Protein ◽  
Bacteriocin Producer ◽  
Origin Region ◽  
Transfer Origin

ABSTRACT The locations of the genetic determinants for enterocin L50 (EntL50A and EntL50B), enterocin Q (EntQ), and enterocin P (EntP) in the multiple bacteriocin producer Enterococcus faecium strain L50 were determined. These bacteriocin genes occur at different locations; entL50AB (encoding EntL50A and EntL50B) are on the 50-kb plasmid pCIZ1, entqA (encoding EntQ) is on the 7.4-kb plasmid pCIZ2, and entP (encoding EntP) is on the chromosome. The complete nucleotide sequence of pCIZ2 was determined to be 7,383 bp long and contains 10 putative open reading frames (ORFs) organized in three distinct regions. The first region contains three ORFs: entqA preceded by two divergently oriented genes, entqB and entqC. EntqB shows high levels of similarity to bacterial ATP-binding cassette (ABC) transporters, while EntqC displays no significant similarity to any known protein. The second region encompasses four ORFs (orf4 to orf7), and ORF4 and ORF5 display high levels of similarity to mobilization proteins from E. faecium and Enterococcus faecalis. In addition, features resembling a transfer origin region (oriT) were found in the promoter area of orf4. The third region contains three ORFs (orf8 to orf10), and ORF8 and ORF9 exhibit similarity to the replication initiator protein RepE from E. faecalis and to RepB proteins, respectively. To clarify the minimum requirement for EntQ synthesis, we subcloned and heterologously expressed a 2,371-bp fragment from pCIZ2 that encompasses only the entqA, entqB, and entqC genes in Lactobacillus sakei, and we demonstrated that this fragment is sufficient for EntQ production. Moreover, we also obtained experimental results indicating that EntqB is involved in ABC transporter-mediated EntQ secretion, while EntqC confers immunity to this bacteriocin.

Smearing of soft cheese with Enterococcus faecium WHE 81, a multi-bacteriocin producer, against Listeria monocytogenes

2009 ◽  
Vol 26 (1) ◽  
pp. 16-20 ◽  
Author(s):  
E IZQUIERDO ◽  
E MARCHIONI ◽  
D AOUDEWERNER ◽  
C HASSELMANN ◽  
S ENNAHAR
Keyword(s):  
Listeria Monocytogenes ◽  
Enterococcus Faecium ◽  
Bacteriocin Producer ◽  
Soft Cheese

Optimization of Fermentation Conditions for Chinese Fermentation Paocai with Enterococcus faecium IN3531 as a Starter by Single Factor Method

2014 ◽  
Vol 884-885 ◽  
pp. 471-474
Author(s):  
Wen Li Liu ◽  
Lan Wei Zhang ◽  
John Shi ◽  
Hua Xi Yi
Keyword(s):  
Enterococcus Faecium ◽  
Starter Culture ◽  
Titratable Acidity ◽  
Inoculum Size ◽  
Single Factor ◽  
Fermentation Time ◽  
Fermentation Conditions ◽  
Bacteriocin Producer ◽  
Optimization Of Fermentation ◽  
Single Factor Experiment

The strain Enterococcus faeciumIN3531, a bacteriocin producer, was used as a starter culture for traditional fermentationpaocai in china. By single factor experiment, the technological conditionsof Chinese fermentation paocai using Enterococcus faecium IN3531 as a starterwere optimizated, andparameters of optimization included the initial saltconcentration, the inoculum size, the fermentation temperatureand the ratio of material to liquid. Theresearch results showed that,the suitable fermentationconditions were the initial saltconcentration of 2%,the inoculum size of 3%, the fermentation temperature of 37 °C, the ratio of materialto liquid of 30%, fermentation time of 108 hours. Titratable acidity in fermentationterminus was 0.80%.

scholarly journals Immunochemical Characterization of Temperature-Regulated Production of Enterocin L50 (EntL50A and EntL50B), Enterocin P, and Enterocin Q by Enterococcus faecium L50

2006 ◽  
Vol 72 (12) ◽  
pp. 7634-7643 ◽  
Author(s):  
Raquel Criado ◽  
Jorge Gutiérrez ◽  
María Martín ◽  
Carmen Herranz ◽  
Pablo E. Hernández ◽  
...  
Keyword(s):  
Enterococcus Faecium ◽  
Polyclonal Antibodies ◽  
Keyhole Limpet Hemocyanin ◽  
Enzyme Linked Immunosorbent Assay ◽  
C Terminus ◽  
Bacteriocin Producer ◽  
Agric Food ◽  
Different Temperatures ◽  
Enterocin P

ABSTRACT Polyclonal antibodies with specificity for enterocin L50A (EntL50A), enterocin L50B (EntL50B), and enterocin Q (EntQ) produced by Enterococcus faecium L50 have been generated by immunization of rabbits with chemically synthesized peptides derived from the C terminus of EntL50A (LR1) and EntL50B (LR2) and from the complete enterocin Q (EntQ) conjugated to the carrier protein keyhole limpet hemocyanin (KLH). The sensitivity and specificity of these antibodies were evaluated by a noncompetitive indirect enzyme-linked immunosorbent assay (NCI-ELISA) and a competitive indirect ELISA (CI-ELISA). The NCI-ELISA was valuable for detecting anti-EntL50A-, anti-EntL50B-, and anti-EntQ-specific antibodies in the sera of the LR1-KLH-, LR2-KLH-, and EntQ-KLH-immunized animals, respectively. Moreover, these antibodies and those specific for enterocin P (EntP) obtained in a previous work (J. Gutiérrez, R. Criado, R. Citti, M. Martín, C. Herranz, M. F. Fernández, L. M. Cintas, and P. E. Hernández, J. Agric. Food Chem. 52:2247-2255, 2004) were used in an NCI-ELISA to detect and quantify the production of EntL50A, EntL50B, EntP, and EntQ by the multiple-bacteriocin producer E. faecium L50 grown at different temperatures (16 to 47°C). Our results show that temperature has a strong influence on bacteriocin production by this strain. EntL50A and EntL50B are synthesized at 16 to 32°C, but production becomes negligible when the growth temperature is above 37°C, whereas EntP and EntQ are synthesized at temperatures ranging from 16 to 47°C. Maximum EntL50A and EntL50B production was detected at 25°C, while EntP and EntQ are maximally produced at 37 and 47°C, respectively. The loss of plasmid pCIZ1 (50 kb) and/or pCIZ2 (7.4 kb), encoding EntL50A and EntL50B as well as EntQ, respectively, resulted in a significant increase in production and stability of the chromosomally encoded EntP.

Demonstration of the cellular viability and safety of Enterococcus faecium CRL 183 in experiments of long term

2007 ◽  
Vol 87 (2) ◽  
pp. 181 ◽  
Author(s):  
Katia Sivieri ◽  
Veridiana P.S. Cano ◽  
Sandro R. Valentini ◽  
Elizeu A. Rossi
Keyword(s):  
Enterococcus Faecium ◽  
Cellular Viability
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