scholarly journals A Confocal Microscopic Image of Three-dimensional Cultured Fibroblasts under Mechanical Forces

2015 ◽  
Vol 11 (4) ◽  
pp. 178-179
Author(s):  
Rei Ogawa
Keyword(s):  
Three Dimensional ◽  
Mechanical Forces ◽  
Microscopic Image ◽  
Cultured Fibroblasts

Three-dimensional Reconstruction of Microscopic Image Based on Multi-ST Algorithm

2020 ◽  
Vol 64 (2) ◽  
pp. 20506-1-20506-7
Author(s):  
Min Zhu ◽  
Rongfu Zhang ◽  
Pei Ma ◽  
Xuedian Zhang ◽  
Qi Guo
Keyword(s):  
3D Reconstruction ◽  
Three Dimensional ◽  
Scale Model ◽  
Microscopic Image ◽  
Multi Scale ◽  
Gaussian Pyramid ◽  
Cost Aggregation ◽  
Dimensional Reconstruction ◽  
Image Pairs ◽  
Accurate Matching

Abstract Three-dimensional (3D) reconstruction is extensively used in microscopic applications. Reducing excessive error points and achieving accurate matching of weak texture regions have been the classical challenges for 3D microscopic vision. A Multi-ST algorithm was proposed to improve matching accuracy. The process is performed in two main stages: scaled microscopic images and regularized cost aggregation. First, microscopic image pairs with different scales were extracted according to the Gaussian pyramid criterion. Second, a novel cost aggregation approach based on the regularized multi-scale model was implemented into all scales to obtain the final cost. To evaluate the performances of the proposed Multi-ST algorithm and compare different algorithms, seven groups of images from the Middlebury dataset and four groups of experimental images obtained by a binocular microscopic system were analyzed. Disparity maps and reconstruction maps generated by the proposed approach contained more information and fewer outliers or artifacts. Furthermore, 3D reconstruction of the plug gauges using the Multi-ST algorithm showed that the error was less than 0.025 mm.

Color Reproduction Accuracy Promotion of 3D-Printed Surfaces Based on Microscopic Image Analysis

2019 ◽  
Vol 34 (01) ◽  
pp. 2054004 ◽  
Author(s):  
Xiaochun Wang ◽  
Chen Chen ◽  
Jiangping Yuan ◽  
Guangxue Chen
Keyword(s):  
Surface Roughness ◽  
Image Analysis ◽  
Three Dimensional ◽  
Prediction Method ◽  
Chromatic Aberration ◽  
Optimization Methods ◽  
Experimental Models ◽  
Microscopic Image ◽  
Color Reproduction ◽  
Microscopic Image Analysis

Full-color three-dimensional (3D) printing technology is a powerful process to manufacture intelligent customized colorful objects with improved surface qualities; however, poor surface color optimization methods are the main impeding factors for its commercialization. As such, the paper explored the correlation between microstructure and color reproduction, then an assessment and prediction method of color optimization based on microscopic image analysis was proposed. The experimental models were divided into 24-color plates and 4-color cubes printed by ProJet 860 3D printer, then impregnated according to preset parameters, at last measured by a spectrophotometer and observed using both a digital microscope and a scanning electron microscope. The results revealed that the samples manifested higher saturation and smaller chromatic aberration ([Formula: see text]) after postprocessing. Moreover, the brightness of the same color surface increased with the increasing soaked surface roughness. Further, reduction in surface roughness, impregnation into surface pores, and enhancement of coating transparency effectively improved the accuracy of color reproduction, which could be verified by the measured values. Finally, the chromatic aberration caused by positioning errors on different faces of the samples was optimized, and the value of [Formula: see text] for a black cube was reduced from 8.12 to 0.82, which is undetectable to human eyes.

Effect of Position and Flow Waveform on the Fluid Mechanics of a Stenosed Human Right Coronary Artery

2001 ◽  
Author(s):  
K. B. Chandran ◽  
S. D. Ramaswamy ◽  
Y.-G. Lai ◽  
A. Wahle ◽  
M. Sonka
Keyword(s):  
Fluid Mechanics ◽  
Three Dimensional ◽  
Coronary Vessels ◽  
Mechanical Forces ◽  
Flow Waveform ◽  
Human Right ◽  
Plaque Growth ◽  
Oscillatory Shear Stress ◽  
The Relationship

Abstract Complete occlusion in any of the coronary vessels leads to a myocardial infarction. The role of fluid mechanical forces in atheroma development has been widely accepted because of preferential plaque growth at certain locations of the vessel geometry, such as a bifurcation or regions of high degrees of curvature. Areas of low and/or oscillatory shear stress have been correlated with atheroma development [1]. In order to determine the relationship between fluid mechanical stresses and development of lesions in the coronary vessels, it is important to analyze the fluid mechanics in actual three-dimensional geometries, incorporating the time-dependent translation and geometric alterations of these vessels [2,3].

scholarly journals Three-Dimensional Modeling of Mechanical Forces in the Extracellular Matrix during Epithelial Lumen Formation

2006 ◽  
Vol 90 (12) ◽  
pp. 4380-4391 ◽  
Author(s):  
Dehong Zeng ◽  
Aldo Ferrari ◽  
Jens Ulmer ◽  
Alexey Veligodskiy ◽  
Peter Fischer ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Three Dimensional ◽  
Mechanical Forces ◽  
Lumen Formation ◽  
Three Dimensional Modeling ◽  
Dimensional Modeling

scholarly journals Multiscale integration of environmental stimuli in plant tropism produces complex behaviors

2020 ◽  
Author(s):  
Derek E Moulton ◽  
Hadrien Oliveri ◽  
Alain Goriely
Keyword(s):  
Three Dimensional ◽  
Feedback Mechanism ◽  
Dynamic Responses ◽  
Three Dimensions ◽  
Mechanical Forces ◽  
Complex Behavior ◽  
Directed Movement ◽  
Cellular Changes ◽  
Hormone Transport ◽  
External Stimuli

Plant tropism refers to the directed movement of an organ or organism in response to external stimuli. Typically, these stimuli induce hormone transport that triggers cell growth or deformation. In turn, these local cellular changes create mechanical forces on the plant tissue that are balanced by an overall deformation of the organ, hence changing its orientation with respect to the stimuli. This complex feedback mechanism takes place in a three-dimensional growing plant with varying stimuli depending on the environment. We model this multiscale process in filamentary organs for an arbitrary stimulus by linking explicitly hormone transport to local tissue deformation leading to the generation of mechanical forces and the deformation of the organ in three dimensions. We show, as examples, that the gravitropic, phototropic, nutational, and thigmotropic dynamic responses can be easily captured by this framework. Further, the integration of evolving stimuli and/or multiple contradictory stimuli can lead to complex behavior such as sun following, canopy escape, and plant twining.

scholarly journals CRT Silencing Inhibits ECM Synthesis in HGFs Cultured on Three-Dimensional PLGA Scaffolds Via Inhibiting The CaN/NFAT3 Signalling Pathway

2021 ◽  
Author(s):  
Hui Wei ◽  
Zhixing Chen ◽  
Hsuyin Min ◽  
Qinqin Ma ◽  
Yuting You ◽  
...  
Keyword(s):  
Quantitative Polymerase Chain Reaction ◽  
Three Dimensional ◽  
Mechanical Force ◽  
Signalling Pathway ◽  
Mechanical Forces ◽  
Matrix Synthesis ◽  
Chain Reaction ◽  
Potential Therapeutic Target ◽  
Polymerase Chain ◽  
The Stability

Abstract The stability of orthodontic treatment is believed to be greatly affected by the compression and contraction of gum tissue, but the underlying molecular mechanism remains unclear. The aim of the current study was to explore the effects of mechanical forces on CRT, CaN, NFAT3, p-NFAT3 and COL-I expression in human gingival fibroblasts (HGFs) cultured on three-dimensional (3D) poly(lactic-co-glycolic acid) (PLGA) scaffolds. A mechanical force of 25 g/cm2 was applied to HGFs for 0, 6, 24, 48, and 72h. The expression of CRT, CaN, NFAT3, p-NFAT3, and COL-I were examined by reverse transcription-quantitative polymerase chain reaction and western blotting analysis. The application of mechanical force on HGFs cultured on the 3D PLGA scaffolds led to a significant increase in CRT, CaN, and COL‑I expression, as well as a reduction in p-NFAT3 expression. The mechanical force effects were reversed by silencing CRT. By lowering the CRT expression, p-NFAT3 was upregulated, while CaN and COL‑I were downregulated in HGFs. These findings suggested that downregulation of CRT inhibited extracellular matrix synthesis, potentially via CaN/NFAT3 signalling pathway. Therefore, CRT may serve as a potential therapeutic target against gingival fibrosis.

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