scholarly journals MORPHOLOGICAL STRUCTURE OF ADRENAL CORTEX IN FULL-TERM NEWBORNS WITH CONGENITAL CYTOMEGALOVIRUS INFECTION

2018 ◽  
Vol 1 (69) ◽  
pp. 70-73
Author(s):  
Игорь Гориков ◽  
Igor Gorikov ◽  
Лариса Сомова ◽  
Larisa Somova ◽  
Ирина Андриевская ◽  
...  
Keyword(s):  
Adrenal Cortex ◽  
Cytomegalovirus Infection ◽  
Comparison Group ◽  
Main Group ◽  
Full Term ◽  
Congenital Cytomegalovirus Infection ◽  
Congenital Cytomegalovirus ◽  
General Plan ◽  
Suprarenal Glands ◽  
Term Newborns

The aim was to study the structure of the adrenal cortex in 36 dead full-term newborns. The main group consisted of 16 newborns with congenital cytomegalovirus infection, clinically manifested by moderate and severe cerebral ischemia, predominance of hypertensive-hydrocephalic syndrome, pseudocysts of the vascular plexus, sub-perpendicular and subarachnoid hemorrhages, monocytosis, vesiculosis, pneumonia, hepatitis and meningoencephalitis. The comparison group consisted of 20 dead newborns from mothers who did not have infectious diseases during pregnancy, as well as moderate and severe somatic and obstetric pathology. Birth trauma, intranatal and postnatal hypoxia were the main cause of their death. The evaluation of the pathomorphological picture of the adrenal cortex included a description of the general plan of the structure, the severity of alterative changes in glandulocytes, the number of adenomatous-like structures and their morphological forms, the reaction of loose fibrous connective tissue. It was found out that in suprarenal glands in children of the main group unlike the comparison group there was often detected a breach of the strands of granulocytes, there were found pronounced alterative changes in the cells; there were revealed large adenomatous-like structures in the lumen of which eosinophilic mass was often found and there was observed strongly-pronounced plethora of vessels. Only in the main group there were determined areas of thinning of the cortical substance of the glands, several adenomatous-like formations and anatomical forms containing in their lumen red blood cells and erythroblasts, as well as large hemorrhages. These structural changes indicated inhibition of adrenal cortex formation and prolonged antenatal stimulation of steroidogenesis under the influence of congenital cytomegalovirus infection leading to a decrease in glucocorticoid function of the suprarenal glands in children after birth.

scholarly journals MORPHOLOGIC CONDITION OF THE LIVER IN NEWBORNS WITH INBORN CYTOMEGALOVIRUS INFECTION

2018 ◽  
Vol 1 (67) ◽  
pp. 60-63
Author(s):  
Наталия Ишутина ◽  
Nataliya Ishutina ◽  
Игорь Гориков ◽  
Igor Gorikov ◽  
Лариса Сомова ◽  
...  
Keyword(s):  
Cytomegalovirus Infection ◽  
Bile Ducts ◽  
Morphological Structure ◽  
Birth Trauma ◽  
Full Term ◽  
Congenital Cytomegalovirus Infection ◽  
Congenital Cytomegalovirus ◽  
General Plan ◽  
Term Newborns ◽  
Portal Tracts

The aim of the study was to study the morphological structure of the liver in newborns with congenital cytomegalovirus infection. The material for the study was the liver of 36 full-term newborns who died on the 2nd-5th day of life from birth trauma, intranatal and postnatal hypoxia. The main study group included 16 full-term newborns with congenital cytomegalovirus infection. The comparison group included 20 newborns from mothers without any viral infections, as well as moderate, severe somatic diseases and obstetric pathology during pregnancy, and these babies died at the 2nd-5th day of life from birth trauma, intrapartum and postnatal hypoxia. The main cause of death of children of early neonatal age was intrauterine infection which was clinically manifested by moderate or severe cerebral ischemia with hypertension-hydrocephalic syndrome, pseudocysts of the vascular plexus, subependymal and subarachnoid hemorrhages, monocytosis, vesiculosis, pneumonia, hepatitis and meningoencephalitis and antenatal hypoxia caused by the development of subcompensated placental insufficiency. When describing the liver, the attention was drawn to the following macroscopic and pathohistological changes in the organ: 1) the condition of the capsule of the organ; 2) the change of the general plan of a structure; 3) the reaction of the bloodstream of the liver: 4) the condition of the portal tracts; 5) the state of the lumen of the bile ducts and its epithelium. The material was fixed in 10% neutral formalin, dehydrated in alcohols and poured into paraffin. For observational microscopy, histological sections 5-7 μm thick were stained with Böhmer hematoxylin and eosin. Morphological examination of the liver in the newborns of the main group showed an increase in the frequency of detection of subcapsular hematomas, pronounced congestion of sinusoids, foci of lymphohistiocytic infiltration of the connective tissue of the portal tracts, small-focal proliferation of Kupffer cells, alterative and proliferative changes in the epithelium of the bile ducts, as well as cells with viral metamorphosis. The revealed structural changes in the morphological structure of the liver in full-term newborns indicate the development of pronounced dyscirculatory, alterative and inflammatory changes in the liver of cytomegalovirus genesis.

scholarly journals Congenital cytomegalovirus infection and its impact on the auditory function

2019 ◽  
Vol 11 (3) ◽  
pp. 83-89
Author(s):  
R. A. Ivanova ◽  
M. Yu. Boboshko ◽  
E. S. Garbaruk ◽  
S. M. Vikhnina ◽  
V. V. Vasiliev ◽  
...  
Keyword(s):  
Hearing Loss ◽  
Sensorineural Hearing Loss ◽  
Cytomegalovirus Infection ◽  
Comparison Group ◽  
Late Onset ◽  
Main Group ◽  
Sensorineural Hearing ◽  
Congenital Cytomegalovirus Infection ◽  
Congenital Cytomegalovirus

Congenital cytomegalovirus infection contributes substantially to the incidence of sensorineural hearing loss, which may be late-onset, progressive or fluctuating. It leads to delayed diagnostics of hearing impairments in children. The aim is to assess the frequency and time of hearing loss occurrence in children with congenital cytomegalovirus infection and to develop the audiological follow-up algorithm adapted for those children. Materials and methods: 60 children with verified congenital cytomegalovirus infection have been involved into research as the main group. 61 children, with other sensorineural hearing loss risk factors, but excluding congenital cytomegalovirus, were included into the comparison group. The age of children ranged from 2 months to 7 years old. The follow-up duration was up to 4 years. Audiological assessment included: auditory brainstem response, auditory steady state response, otoacoustic emissions, impedancometry, pure tone audiometry (its type depended on the children’s age and development level) and speech tests. Results: Hearing loss was revealed in 17% of the main group, it varied from mild to severe degree. Among these children 30% developed late-onset hearing loss. 5% of children of the comparison group were identified with congenital hearing loss. Assessment of central auditory pathways function has been performed in children elder than 4 years old: 70% of children of the main group and 10% of children of the comparison one were identified with auditory processing disorders. The received data may be used by otolaryngologists, audiologists, pediatricians and infectious diseases physicians. The developed follow-up admits timely diagnostics of sensorineural hearing loss in children with congenital cytomegalovirus.

Congenital cytomegalovirus infection in preterm and full-term newborn infants from a population with a high seroprevalence rate

2001 ◽  
Vol 20 (2) ◽  
pp. 188-192 ◽  
Author(s):  
APARECIDA YULIE YAMAMOTO ◽  
MARISA M??RCIA MUSSI-PINHATA ◽  
PATRICIA CRISTINA GOMES PINTO ◽  
LUIZ TADEU MORAES FIGUEIREDO ◽  
SALIM MOYS??S JORGE
Keyword(s):  
Cytomegalovirus Infection ◽  
Newborn Infants ◽  
Full Term ◽  
Seroprevalence Rate ◽  
High Seroprevalence ◽  
Congenital Cytomegalovirus Infection ◽  
Congenital Cytomegalovirus ◽  
Term Newborn

scholarly journals ECHOSTRUCTURE AND PATHOMORPHOLOGICAL CHARACTERISTICS OF THE CEREBROSPINAL FLUID SPACE OF THE BRAIN IN NEWBORNS WITH CONGENITAL CYTOMEGALOVIRUS INFECTION

2019 ◽  
Vol 1 (71) ◽  
pp. 94-99
Author(s):  
Игорь Гориков ◽  
Igor Gorikov ◽  
Лариса Сомова ◽  
Larisa Somova ◽  
Ирина Андриевская ◽  
...  
Keyword(s):  
Cytomegalovirus Infection ◽  
Morphological Study ◽  
Lymphocytic Infiltration ◽  
Congenital Cytomegalovirus Infection ◽  
Intrauterine Development ◽  
Vascular Plexus ◽  
Congenital Cytomegalovirus ◽  
Perivascular Edema ◽  
Term Newborns ◽  
The Brain

In 36 full-term newborns who died at an early neonatal age under various conditions of intrauterine development, ultrasound examination and morphological study of the brain liquor space was conducted at the 1st-2nd days of life. The first group included 20 newborns aged 38-40 weeks, whose cause of death on the 4th -5th day of life was intranatal and postnatal hypoxia. The second group included 16 dead children of the same age with congenital cytomegalovirus infection. It was found out that immaturity (n=7, p<0.05), pseudocysts of the vascular plexus of the lateral ventricles (n=9, p<0.5), as well as intraventricular hemorrhages of I degree (n=8, p<0.05) were more often diagnosed in the second group compared with the first one in neurosonographic study. Only in the second group there was a pronounced ventriculomegaly. A morphological study in the brain often detected a pronounced pericellular and perivascular edema, alterative changes of neurons, vasculitis (n=7), subependyma (n=3), choriocephalitis (n=3) and encephalitis (n=1) with small calcifications, ependymocyte desquamation and lymphocytic infiltration, with a pronounced hyperemia and hemorrhages. In all cases, specific cells of the "owl's eye" were not detected. The detected ultrasound and pathomorphological changes in the liquor space in intrauterine cytomegalovirus infection indicate the possibility of direct cytodestructive effect of the pathogen in the absence of markers of viral metamorphosis, as well as the indirect effect of antenatal hypoxia, endotoxemia and cytokineemia on the brain in newborns

scholarly journals PATHOMORPHOLOGIC CHARACTERISTIC OF BRONCHOPULMONARY SYSTEM IN THE DIED NEWBORNS WITH THE INBORN CYTOMEGALOVIRUS INFECTION

2018 ◽  
Vol 1 (68) ◽  
pp. 63-67
Author(s):  
Игорь Гориков ◽  
Igor Gorikov ◽  
Лариса Сомова ◽  
Larisa Somova ◽  
Ирина Андриевская ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cytomegalovirus Infection ◽  
Viral Infections ◽  
Comparison Group ◽  
Morphological Structure ◽  
Bronchial Epithelium ◽  
Bronchial Tree ◽  
Main Group ◽  
Vascular Congestion ◽  
Term Newborns

The aim of the research was to study a morphological structure of airway and respiratory parts of lungs in 36 full-term newborns who died at the 2-5th day of life. The main group included 16 died newborns with the inborn cytomegalovirus infection who were diagnosed with moderate to severe cerebral ischemia with hypertension-hydrocephalic syndrome, pseudocysts of the blood vessels plexus, subependymal and subarachnoid haemorrhages, monocytosis, vesiculitis, pneumonia, hepatitis and meningocephalitis against intrauterine hypoxia and chronic subcompensated placental insufficiency. The comparison group consisted of 20 newborns whose mothers during the gestation period did not have acute respiratory viral infections, moderate and severe somatic and obstetrical pathology. The cause of their death was a birth trauma, intranatal and postnatal hypoxia. Describing lungs the following morphologic features were emphasized: 1) the changes of the general composition of the organ; 2) the state of the lumen of the large, medial and small bronchi; 3) the reaction of the epithelial lining, conjunctive tissue and the blood channel of the bronchial tree and aero-hematic barrier. As a result of the studies it was found out that in the newborns of the main group in comparison with the children of the comparison group in the lumen of large bronchi there was a rare case of mucus, there were often found erythrocytes and desquamated epithelial cells. Only infected children were found to have leucocytes. More intensified were alterative processes in epithelial cells; there were often found cells of the bronchial epithelium without ciliary structures, desquamation of highly differential ciliary and goblet cells, cell elements with a small hyperchromic nucleus and light cytoplasm in the cambial layer, sharply expressed vascular congestion, proliferative changes in the bronchial epithelium as well as hydropic-hemorrhagic and aspiration syndromes. Pneumonia and signs of the virus metamorphosis were diagnosed only in the main group.

scholarly journals Changes in lipid-synthesizing function of the liver in congenital cytomegalovirus infection in death newborns

2021 ◽  
pp. 79-83
Author(s):  
I. N. Gorikov ◽  
L. G. Nakhamchen ◽  
A. N. Оdireev ◽  
E. I. Karapetyan ◽  
T. V. Smirnova ◽  
...  
Keyword(s):  
Total Cholesterol ◽  
Indirect Effect ◽  
Cytomegalovirus Infection ◽  
Morphological Structure ◽  
High Density ◽  
High Density Lipoproteins ◽  
Congenital Cytomegalovirus Infection ◽  
Congenital Cytomegalovirus ◽  
Triglyceride Concentration ◽  
Term Newborns

Aim. To assess the change in the lipid-synthesizing function of the liver in congenital cytomegalovirus infection (CMVI) in dead newborns.Materials and methods. The study of the lipid spectrum of umbilical cord blood in the first minutes of life was carried out in 59 full-term newborns with antenatal ontogenesis, unburdened and burdened with congenital CMVI. The first group consisted of 25 newborns of early neonatal age (control group) from mothers without moderate and severe obstetric and somatic pathology, as well as respiratory viral diseases and sexually transmitted infections. The second group included 18 children of the same age (comparison group), whose mothers suffered exacerbation of CMVI in the second trimester of pregnancy, which did not lead to antenatal viral aggression. The third group was represented by 16 newborns with congenital CMVI. The life expectancy of children was 2-5 days. In the first group, the main cause of death of children was prolonged ante- and intranatal hypoxia, atelectasis and hyaline membranes of the lungs, in the second group – prolonged antenatal hypoxia, intranatal hypoxia and atelectatic, hyaline and edematous-hemorrhagic pneumopathies, and in the third group – congenital CMVI, which manifested itself as cerebral ischemia of moderate and severe degree, meningoencephalitis, ventriculomegaly, pseudocysts of the vascular plexus and subarachnoid hemorrhages, monocytosis, vesiculopustulosis, hepatitis and pneumonia. In the blood serum from the umbilical vein during biochemical analysis, the concentration (mmol/L) of total cholesterol, high-density lipoproteins, low-density lipoproteins and triglycerides was estimated.Results. In newborns of the second group in the blood serum from the umbilical cord vein, the concentration of total cholesterol was 1.90±0.04 mmol/L, high-density lipoproteins – 1.49±0.06 mmol/L, low-density lipoproteins - 0.26±0,02 mmol/L and triglycerides – 0.49±0.03 mmol/L (in the first group, respectively, 1.93±0.06 mmol/L, p>0.05; 1.37±0.06 mmol/L, p>0.05; 0.43±0.02 mmol/L, p<0.001; 0.45±0.02 mmol/L, p>0.05). In the third group, compared with the second one, there was a decrease in the level of total cholesterol to 1.79±0.04 mmol/L (p<0.05) and high-density lipoproteins – to 1.28±0.06 (p><0.05) against the background of an increase in triglyceride concentration up to 0.59±0.03 mmol/L (p><0.05). Conclusion. The above changes in the lipid-synthesizing function of the liver indicate a direct and indirect effect of congenital CMVI on the morphological structure of hepatocytes and the activity of enzyme systems. Key words: full-term newborns, congenital cytomegalovirus infection, total cholesterol, high-density lipoproteins, lowdensity lipoproteins, triglyceride>˂ 0.05) and high-density lipoproteins – to 1.28±0.06 (p<0.05) against the background of an increase in triglyceride concentration up to 0.59±0.03 mmol/L (p><0.05). Conclusion. The above changes in the lipid-synthesizing function of the liver indicate a direct and indirect effect of congenital CMVI on the morphological structure of hepatocytes and the activity of enzyme systems. Key words: full-term newborns, congenital cytomegalovirus infection, total cholesterol, high-density lipoproteins, lowdensity lipoproteins, triglycerides>˂ 0.05) against the background of an increase in triglyceride concentration up to 0.59±0.03 mmol/L (p˂ 0.05).Conclusion. The above changes in the lipid-synthesizing function of the liver indicate a direct and indirect effect of congenital CMVI on the morphological structure of hepatocytes and the activity of enzyme systems. 

scholarly journals About the absence of the need to prescribe antibacterial therapy to newborns from mothers with clinical chorioamnionitis

2021 ◽  
Vol 12 (3) ◽  
pp. 5-14
Author(s):  
Ekaterina A. Shevareva ◽  
Larisa A. Fedorova ◽  
Oksana V. Nevmerzhitskaia
Keyword(s):  
Antibiotic Therapy ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Comparison Group ◽  
Main Group ◽  
Full Term ◽  
Laboratory Monitoring ◽  
Term Newborns

Background. The diagnosis of a woman in labor chorioamnionitis (CA) implies a high risk of infectious complications for the mother and fetus, which determines the need for additional examination of infants and the decision on the appointment of antibacterial therapy. The purpouse of this study was determine the need to administration antibiotic therapy to full-term newborns from mothers diagnosed with chorioamnionitis. Materials and methods. 113 full-term newborns were examined, of which the main group consisted of children whose mothers were diagnosed with chorioamnionitis (n = 77), the comparison group children born to healthy mothers (n = 36). All children performed clinical and laboratory monitoring, including a clinical analysis of capillary blood in the first 24 hours of life; determination of the level of C-reactive protein (CRP) in venous blood on the 3rd day of life. Bacteriological examination of newborns included sampling of material from the ear fold, buccal mucosa, umbilical cord blood, as well as the contents of the tracheobronchial tree (TBD) during respiratory therapy with mechanical ventilation. Special research methods included studies of the proinflammatory cytokines (IL-1, IL-6) in umbilical cord blood. Histological CA was diagnosed in the presence of morphological and functional signs of inflammation in the placenta. Results. Newborns of the main group more often developed respiratory disorders requiring respiratory and oxygen therapy (p = 0,045). The production of IL-1, IL-6 in umbilical cord blood in the examined newborns of the main group was higher than in the comparison group [Odds Ratio (OR) 8.4; 95% Confidence Interval (CI): 1.067.9; OR 7.4; 95% CI: 2.521.7 respectively]. The study of blood samples revealed leukocytosis (34109) 6.5% vs 0%, p 0.05) and a shift in the leukocyte count to young forms of neutrophils (45.4% vs 16.7%, p 0.05) in the peripheral blood of infants of the main group. Infants exposed to maternal clinical chorioamnionitis had increased level of CRP 10.3 times more frequent (95% CI: 2.837.1) than in newborns in the comparison group. With dynamic clinical and laboratory monitoring, 72 children of the main group (93.5%) had no data for the course of the infection, as a result of which they did not receive antibiotic therapy. Conclusion: Administration antibiotic therapy to clinically healthy full-term newborns from mothers diagnosed with chorioamnionitis is unjustified. Infants of this group require clinical laboratory, dynamic observation with laboratory control, including a clinical blood test and determination of the CRP level, which is a preferred alternative to the appointment of antibiotic therapy.

scholarly journals MORPHOLOGICAL STRUCTURE OF THE THYMUS IN NEWBORNS WITH CONGENITAL CYTOMEGALOVIRUS INFECTION

2018 ◽  
Vol 1 (69) ◽  
pp. 64-69
Author(s):  
Ирина Андриевская ◽  
Irina Andrievskaya ◽  
Игорь Гориков ◽  
Igor Gorikov ◽  
Лариса Сомова ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Blood Vessels ◽  
Cytomegalovirus Infection ◽  
Comparison Group ◽  
Morphological Structure ◽  
Main Group ◽  
The Body ◽  
Thymus Gland ◽  
Fibrous Connective Tissue ◽  
General Plan

The aim of the work was to study the morphological structure of the thymus gland in 36 full-term newborns who died at the 2-5 day of life. The main group included 16 newborns who were diagnosed with intrauterine cytomegalovirus infection by the serological method and polymerase chain reaction. Cerebral ischemia of moderate and severe degree, subarachnoid and periventricular hemorrhages, as well as markers of local and generalized forms of congenital infection were revealed in children of this group. The comparison group was represented by 20 newborns with antenatal ontogenesis, uncomplicated by acute respiratory and herpes-virus infections, moderate and severe somatic and obstetric pathology in their mothers during pregnancy. The cause of their death was birth trauma, intranatal and postnatal hypoxia. In the description of the neonatal thymus, attention was paid to the following features: the general plan of the structure; structural changes in the capsule of the gland; the structure of the cortical and brain matter; violation of loose fibrous connective tissue and blood vessels of the organ. It was found out that the structure of the thymus gland in the main group was different from that in the comparison group, especially a predominance of neonates with increase and decrease in the organ mass. With the growth of the mass of the thymus there was frequently revealed subcapsular hemorrhage; the sharpness of borders decreased or completely disappeared between cortical and medullary substance; the areas of crust delymphatisation, Hassall’s corpuscles filled with detritus were often identified. The edema of the interlobular loose fibrous connective tissue increased, the endothelial edema and its desquamation in the lumen of blood vessels were noted. Lymphocyte clusters in the interlobular connective tissue were clearly visualized. In the case of a decrease in the body mass, small lobules, excessive formation of collagen fibers in the interlobular connective tissue, the loss of lymphocytes in the cortical substance and small Hassal’s bodies were more often recorded. Stimulation of thymus growth may be associated with the direct cytodestructive effect of the virus and intrauterine stress, leading to a drop in cortisol content. In the inhibition of immunopoesis growth, prolonged antenatal antigenic stimulation, higher level of cortisol against progressing alterative processes in glandulocyte of the cortex of suprarenal glands of the fetus play an important role.

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