Morphometric Changes Due to β Carotene on Wistar Rats Fed Dietary Fat

2018 ◽  
Vol 4 (6) ◽  
pp. 16-20
Author(s):  
Getrude N. Okechukwu ◽  
Nweke B. Ofobuike ◽  
Gabriel G. Akunna
Keyword(s):  
Dietary Fat ◽  
Wistar Rats ◽  
Β Carotene

Exploring the Pharmacognostic Features, Anti-oxidant and Lipid Lowering Potential of Fagopyrum esculentum Moench. Seed

2020 ◽  
Vol 6 (2) ◽  
pp. 155-169
Author(s):  
Neeraj Panihar ◽  
Neeru Vasudeva ◽  
Sunil Sharma ◽  
Babu Lal Jangir
Keyword(s):  
Ethyl Acetate ◽  
Wistar Rats ◽  
Ethanol Extract ◽  
Water Soluble ◽  
Fagopyrum Esculentum ◽  
Lipid Lowering ◽  
Standard Drug ◽  
Fagopyrum Esculentum Moench ◽  
Β Carotene

Background: Fagopyrum esculentum Moench. is a herb consumed as food and has medicinal value. It is a rich source of bioactive nutrients which cure and prevent many ailments. Traditionally, it is used to treat hypertension, diabetes, constipation, cancer etc. Methods and Objective: Present work illustrates morphological, microscopic and physicochemical parameters of Fagopyrum esculentum seeds as per WHO guidelines, in vitro antioxidant activity; assessed by DPPH scavenging method, hydrogen peroxide scavenging assay and β-carotene linoleic acid bleaching method and study of lipid lowering potential of the ethyl acetate and ethanol extract of seeds on normal diet fed Wistar rats. Results: Morphological studies delineated the triangular shape, dark brown colour, 8 mm length and 6 mm width of the seed. The microscopic examination of the transverse section of seed depicted features like testa or pericarp (seed coat), the endosperm, embryo and sclerenchyma cells. Study of physiochemical parameters exhibited 0.3±0.02% of foreign matter and 1.44±0.51% crude fibre content. Total ash, acid insoluble ash and water soluble ash value were 6.7±1.7%, 1.9±0.23% and 3.9± 0.31% respectively. Alcohol soluble and water soluble extractive value came out to be 65.02± 3.21 mg/g and 12.7±1.24 mg/g respectively. Foaming index was less than 100, swelling index was found to be 0.5±0.01 ml/g. Loss on drying was 4.02±1.27%. Phytochemical screening of ethyl acetate and ethanol extract revealed the presence of alkaloids, carbohydrates, phenolic compounds, phytosterols and flavonoids. Trace amount of heavy metals (arsenic, cadmium, lead, mercury) were determined by atomic absorption spectrophotometer. Pesticide residue analysis confirmed the presence of nontoxic pesticides like dimethipin, hymexazol, phenothrin-2, methoprene, triadimenol, prohydrojasmon- 1, jasmolin ii, triademinol, jasmolin i, prohydrojasmone i, cyromazine in both the extracts by gc-ms spectrometer. The ethyl acetate and ethanol extract has shown significant in-vitro antioxidant activities demonstrated by the DPPH method (IC50 = 94.37±2.51 and 216.04±4.39 μg/ml respectively), hydrogen peroxide scavenging assay (IC50 = 83.72±3.72 and 193.47±5.05 µg/ml respectively) and β-carotene bleaching method (IC50 = 100.67±4.01 and 205.39±2.89 µg/ml respectively). Lipid lowering study performed on Wistar rats demonstrated a significant (p<0.001) decrease in serum Total Cholesterol (TC), Triglyceride (TG) and increase in High Density Lipoprotein (HDL) level as compared to normal group. Both the extracts have shown a non significant difference in the level of TG as compared to standard drug atorvastatin, depicting that the efficacy of extracts is at par with that of standard drug atorvastatin. Conclusion: Pharmacognostical study of the plant can be a very good tool for identification as well as authentication of a herb. Moreover, these parameters may be helpful in the development of monograph of the plant. Pharmacological activity confirmed Fagopyrum esculentum Moench. seed to be a good antioxidant and have lipid lowering potential.

Influence of Dietary Fat on β-Carotene Absorption and Bioconversion into Vitamin a

2002 ◽  
Vol 60 (4) ◽  
pp. 104-110 ◽  
Author(s):  
Judy D. Ribaya-Mercado
Keyword(s):  
Vitamin A ◽  
Dietary Fat ◽  
Β Carotene

scholarly journals Effect of conjugated linoleic acid and different type of dietary fat on serum lipid profile, liver enzymes activity and oxidative stress markers in wistar rats

2018 ◽  
pp. 27-33
Author(s):  
Magdalena Franczyk-Żarów ◽  
Edyta Kuś ◽  
Renata B. Kostogrys
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Lipid Profile ◽  
Conjugated Linoleic Acid ◽  
Significant Influence ◽  
Dietary Fat ◽  
Serum Lipid ◽  
Wistar Rats ◽  
Liver Enzymes ◽  
The Body

Background. Nutritional recommendations emphasize the need to limit consumption of saturated fatty acids and to increase the intake of polyunsaturated fatty acids in the prevention of non-communicable chronic diseases, particularly cardiovascular diseases. Among the fatty acids with health-related effects on the body, conjugated fatty acids are mentioned (i.e. CLA). Objective. The current study was designed to determine the effects of conjugated linoleic acid (CLA) on serum lipid profile, glucose, liver enzymes activity (AST and ALT), malonic dialdehyde (MDA) as well as lipid hydroperoxide (LPO) concentrations in rats fed diet differing in type of dietary fat. Material and methods. Male Wistar rats were divided into six groups and fed the following diets: control AIN-93G diet contained soybean oil (O) and diets with modification of fat source: butter (B) and margarine (M). The experimental diets were supplemented with 1% of conjugated linoleic acid (O+CLA, B+CLA, M+CLA). After 21 days the blood was collected and lipid profile, glucose, liver enzymes, MDA as well as LPO were analyzed. Results. The dietary treatments had no significant effect on the body weight and liver weight of the animals. The concentrations of total cholesterol (TC) and LDL+VLDL cholesterol were unchanged. Both experimental factors (fat source and CLA) had a significant influence on the TAG and HDL levels. Margarine (M) significantly increased the TAG concentration, whereas CLA had a significant impact on the TAG reduction (M+CLA). Glucose level was significantly decreased in all groups fed diets supplemented with CLA. Serum ALT significantly increased in all CLA groups. Fat source had statistically significant influence on the MDA concentration. The LPO level was significantly elevated in all CLA groups. There was statistically significant interaction of experimental factors (fat source and CLA supplementation) on LPO level. Conclusions. Margarine had an adverse effect on the rat’s lipid profile. However, in the group fed with margarine, the addition of CLA decreased the concentration of TAG. Regardless of the type of the dietary fat, CLA supplementation increased the level of LPO in the blood serum of animals.

β-carotene inhibits NF-κB and restrains diethylnitrosamine-induced hepatic inflammation in Wistar rats

2020 ◽  
pp. 1-10
Author(s):  
Uzma Latief ◽  
Riaz Ahmad
Keyword(s):  
Liver Fibrosis ◽  
Wistar Rats ◽  
Hepatic Inflammation ◽  
Tissue Sections ◽  
Plausible Mechanism ◽  
Biochemical Mechanisms ◽  
Experimental Liver ◽  
Picrosirius Red ◽  
Β Carotene

Abstract. β-Carotene exhibits antioxidant and hepatoprotective activities via a multitude of biochemical mechanisms. However, the action mechanism involved in antioxidant and anti-inflammatory effects of this carotene in chronic liver diseases is not fully understood. In the present investigation, we have attempted to outline a plausible mechanism of β-carotene action against liver fibrosis in albino Wistar rats. To induce hepatic fibrosis, diethylnitrosamine (DEN) was administered in experimental rats for two weeks. DEN treated rats were divided into four groups, wherein each group comprised of five rats. β-Carotene supplement attenuated DEN-induced elevation in LFT markers (P < 0.05); averted depletion of glycogen (24%, P < 0.05) and, increased nitrite (P < 0.05), hydroxyproline (~67%, P < 0.05) and collagen levels (~65%, P < 0.05). Confocal microscopy of tissue sections stained with picrosirius red revealed accrued collagen in DEN-administered group, which was found to be reduced by β-carotene supplementation. Furthermore, β-carotene decreased the expression of iNOS/NOS-2 and NF-κB, as revealed by immunohistochemistry and Western immunoblotting. Collectively, these results demonstrate that β-carotene mitigates experimental liver fibrosis via inhibition of iNOS and NF-κB in-vivo. Thus, β-carotene may be suggested as a possible nutraceutical to curb experimental liver fibrosis.

scholarly journals Serum Response of Ponies to β-Carotene Fed by Grass Meal or a Synthetic Beadlet Preparation with and without Added Dietary Fat

2002 ◽  
Vol 132 (6) ◽  
pp. 1774S-1775S ◽  
Author(s):  
Ellen Kienzle ◽  
Christine Kaden ◽  
Peter P. Hoppe ◽  
Birgit Opitz
Keyword(s):  
Dietary Fat ◽  
Serum Response ◽  
Β Carotene

scholarly journals Sweet Potato β-Carotene Bioefficacy Is Enhanced by Dietary Fat and Not Reduced by Soluble Fiber Intake in Mongolian Gerbils

2008 ◽  
Vol 139 (1) ◽  
pp. 44-50 ◽  
Author(s):  
Jordan P. Mills ◽  
Gaston A. Tumuhimbise ◽  
Kazi M. Jamil ◽  
Sagar K. Thakkar ◽  
Mark L. Failla ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Dietary Fat ◽  
Mongolian Gerbils ◽  
Fiber Intake ◽  
Soluble Fiber ◽  
Β Carotene

The Effect of Orlistat, an Inhibitor of Dietary Fat Absorption, on the Pharmacokinetics of β-Carotene in Healthy Volunteers

1996 ◽  
Vol 36 (2) ◽  
pp. 152-159 ◽  
Author(s):  
Jianguo Zhi ◽  
Angela T. Melia ◽  
Susan G. Koss-Twardy ◽  
Sujata Arora ◽  
Indravadan H. Patel
Keyword(s):  
Healthy Volunteers ◽  
Dietary Fat ◽  
Fat Absorption ◽  
Dietary Fat Absorption ◽  
Β Carotene
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