scholarly journals Effect of conjugated linoleic acid and different type of dietary fat on serum lipid profile, liver enzymes activity and oxidative stress markers in wistar rats

2018 ◽  
pp. 27-33
Author(s):  
Magdalena Franczyk-Żarów ◽  
Edyta Kuś ◽  
Renata B. Kostogrys
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Lipid Profile ◽  
Conjugated Linoleic Acid ◽  
Significant Influence ◽  
Dietary Fat ◽  
Serum Lipid ◽  
Wistar Rats ◽  
Liver Enzymes ◽  
The Body

Background. Nutritional recommendations emphasize the need to limit consumption of saturated fatty acids and to increase the intake of polyunsaturated fatty acids in the prevention of non-communicable chronic diseases, particularly cardiovascular diseases. Among the fatty acids with health-related effects on the body, conjugated fatty acids are mentioned (i.e. CLA). Objective. The current study was designed to determine the effects of conjugated linoleic acid (CLA) on serum lipid profile, glucose, liver enzymes activity (AST and ALT), malonic dialdehyde (MDA) as well as lipid hydroperoxide (LPO) concentrations in rats fed diet differing in type of dietary fat. Material and methods. Male Wistar rats were divided into six groups and fed the following diets: control AIN-93G diet contained soybean oil (O) and diets with modification of fat source: butter (B) and margarine (M). The experimental diets were supplemented with 1% of conjugated linoleic acid (O+CLA, B+CLA, M+CLA). After 21 days the blood was collected and lipid profile, glucose, liver enzymes, MDA as well as LPO were analyzed. Results. The dietary treatments had no significant effect on the body weight and liver weight of the animals. The concentrations of total cholesterol (TC) and LDL+VLDL cholesterol were unchanged. Both experimental factors (fat source and CLA) had a significant influence on the TAG and HDL levels. Margarine (M) significantly increased the TAG concentration, whereas CLA had a significant impact on the TAG reduction (M+CLA). Glucose level was significantly decreased in all groups fed diets supplemented with CLA. Serum ALT significantly increased in all CLA groups. Fat source had statistically significant influence on the MDA concentration. The LPO level was significantly elevated in all CLA groups. There was statistically significant interaction of experimental factors (fat source and CLA supplementation) on LPO level. Conclusions. Margarine had an adverse effect on the rat’s lipid profile. However, in the group fed with margarine, the addition of CLA decreased the concentration of TAG. Regardless of the type of the dietary fat, CLA supplementation increased the level of LPO in the blood serum of animals.

Serum lipid and electrolyte profiles of Wistar rats fed with Vernonia amygdalina supplemented Vigna subterranea (Bambara groundnut) pudding

2020 ◽  
Vol 3 ◽  
pp. 40-45
Author(s):  
Godwin E. Egbung ◽  
Nyakno A. Essien ◽  
John E. Mgbang ◽  
Josephine E. Egbung
Keyword(s):  
Lipid Profile ◽  
Normal Control ◽  
Serum Lipid ◽  
Wistar Rats ◽  
Total Cholesterol Level ◽  
Bambara Groundnut ◽  
The Body ◽  
Serum Lipid Profile ◽  
Vernonia Amygdalina ◽  
Group 2

Objective: This study investigated the effects of Vernonia amygdalina supplementation on Vigna subterrenea (bambara groundnut) pudding on serum lipid profile and electrolyte levels in Wistar rats. Materials and Methods: Twenty five weanling rats were randomly selected into 5 groups of 5 rats each. Group 1 received normal rat pellet. Groups 2 and 3 received 10% and 5% (w/w) non supplemented bambara pudding, respectively. Groups 4 and 5 were fed with 10% and 5% (w/w) Vernonia supplemented bambara pudding, respectively, for 21 days. The animals were sacrificed using anaesthesia at the end of the experiment and blood sample collected for serum lipid profile and serum electrolyte determinations using standard methods. Results: There was a significant increase (p<0.05) in total cholesterol level in groups 2 (1.64 ± 0.01 mmol/L) and 4 (1.66 ± 0.01 mmol/L) compared to normal control (1.31 ± 0.02 mmol/L). Triacylglycerol level increased significantly in groups 2, 3, and 4 compared to normal control. The high density lipoprotein cholesterol (HDL-c) and Serum sodium level were significantly increased (p < 0.05) in group 2 and 4 respectively compared to the normal control. Potassium, chloride, bicarbonate and calcium levels showed significant increases in group 2 relative to normal control. Phosphate level decreased significantly in group 2 (1.37 ± 0.03 mmol/L), 3 (1.43 ± 0.03 mmol/L) and 5 (1.37 ± 0.03 mmol/L) when compared to normal control (1.75 ± 0.03 mmol/L) respectively. Conclusion: Consumption of bambara groundnut pudding supplemented with Vernonia leaves increases HDL-c level as well as supply appreciable amounts of electrolytes in the body.

scholarly journals Diabetes Mellitus, Nonalcoholic Fatty Liver Disease, and Conjugated Linoleic Acid (Omega 6): What Is the Link?

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Mona Hegazy ◽  
Naglaa M. Elsayed ◽  
Hala M. Ali ◽  
Hanan G. Hassan ◽  
Laila Rashed
Keyword(s):  
Insulin Resistance ◽  
Fatty Acids ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Essential Fatty Acids ◽  
The Body ◽  
Diabetic Patients ◽  
Obesity And Diabetes ◽  
Omega 6

Background and Objective. Type 2 DM and obesity are the coming epidemics and their association with NAFLD is well established; essential fatty acids are vital for body health yet the body cannot make them; 2 essential fatty acids are especially important: linoleic (omega-6) and alpha-linoleic (omega-3) acids; they can be considered as “bioactive lipids” and serve as functional foods. Methods. 50 type 2 Egyptian diabetic patients controlled on oral hypoglycemic drugs together with 20 age- and sex-matched healthy participants were enrolled in the study; all were subjected to complete history taking, BMI, fasting plasma glucose, HOMA-IR, ALT, AST, GGT, urea and creatinine, total lipid profile, hepatitis markers including hepatitis B surface antigen and hepatitis C virus antibodies, conjugated linoleic fatty acid “CLA,” and abdominal ultrasound for grading of NAFLD. Results. Our study in Egyptian diabetics with NAFLD revealed a low level of serum CLA compared to healthy control; such deficiency was more marked with advanced grades of NAFLD; lowest levels were observed in those with severe steatosis (NASH) with definite association between CLA and obesity. Conclusion. Insulin resistance is the main link between NAFLD, diabetes, and obesity. Conjugated linoleic acid (CLA) has a role in fat deposition in the liver and in development and improvement of insulin resistance. Fatty food had a documented role in the pathogenesis of obesity and diabetes but it can also be the cure.

Double enrichment of chicken eggs with conjugated linoleic acid and n-3 fatty acids through dietary fat supplementation

2008 ◽  
Vol 144 (3-4) ◽  
pp. 315-326 ◽  
Author(s):  
P. Cachaldora ◽  
P. García-Rebollar ◽  
C. Alvarez ◽  
J. Méndez ◽  
J.C. De Blas
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Dietary Fat ◽  
Fat Supplementation ◽  
Chicken Eggs

scholarly journals Effects of Dietary Conjugated Linoleic Acid on Broiler Performance and Carcass Characteristics

2017 ◽  
Vol 9 (5) ◽  
pp. 208
Author(s):  
Kátia Maria Cardinal ◽  
Mariana Lemos De Moraes ◽  
Rodrigo Borille ◽  
Gustavo Dias Lovato ◽  
Marcos Speroni Ceron ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Lipid Profile ◽  
Conjugated Linoleic Acid ◽  
Saturated Fatty Acids ◽  
Carcass Characteristics ◽  
Carcass Composition ◽  
Broiler Performance ◽  
Dietary Conjugated Linoleic Acid ◽  
And Performance

The effects of three levels of conjugated linoleic acid dietary inclusion on the carcass characteristics and performance of broilers were evaluated. A total of 405 chickens were raised from 1 until 42 days of age, housed in a room with water and food ad libitum. The experimental design was completely randomized, with three treatments (0.0, 0.5 and 1% CLA) and nine replications (pen) to performance analysis, 18 replications (two birds per pen) to carcass composition, and five replications (left legs) to lipid profile. Performance was determined weekly and after 42 days, 18 birds per treatment were slaughtered to quantify breast and leg yield. Protein and fat was quantified in the leg and breast, as well as the detailed lipid profile of the leg. Data were analyzed by ANOVA and means compared by LS means. From 1 to 21 days chickens with 0% supplementation of CLA performed better compared to those receiving 0.5 and 1% CLA (P < 0.05), however, these differences were no longer significant from 21 to 42 days or for the overall study period (P > 0.05). Conjugated linoleic acid inclusion did not influence leg, breast and carcass yield, and leg and breast content of protein and fat. Both levels of CLA changed the leg lipid profile: there was an increased accumulation of CLA in meat, increased levels of saturated fatty acids and reduction of polyunsaturated fatty acids. Conjugated linoleic acid supplementation increased n-6:n-3 ratio. CLA supplementation in broiler feed is effective to produce meat enriched with its isomers and change lipid profile.

scholarly journals Duration of exposure dependent effect of carbamate treated net on serum lipid profile of Wistar rats

2021 ◽  
Vol 27 (1) ◽  
pp. 27-34
Author(s):  
R.U. Ukpanukpong ◽  
L.K. Eban ◽  
G.I. Ekpo ◽  
U.I. Aletan ◽  
D.D. Asuquo
Keyword(s):  
Body Weight ◽  
Lipid Profile ◽  
Serum Lipid ◽  
Wistar Rats ◽  
Density Lipoprotein ◽  
The Body ◽  
Lipoprotein Cholesterol ◽  
Serum Lipid Profile ◽  
Control Group ◽  
Dependent Effect

The duration of exposure dependent effect of Carbamate treated net on serum lipid profile of female Wistar rats was investigated in this study. Eighteen (18) animals were randomly divided into three (3) study groups with six (6) rats each. Group 1 was designated as the control group while groups 2 and 3 constituted the experimental groups containing rats exposed to Carbamate treated net for 30 and 60 days respectively. The rats were exposed daily to Carbamate treated net by whole body and nose inhalation mode of exposure. The body weight of rats was measured at the commencement of the experiment and at the end of the experiment. The organ weight result obtained indicated that the body weight increased significantly in the rats of the experimental groups when compared to the rats in the control group at p<0.05. The result obtained indicated that the body weight increased significantly in the rats of the experimental groups when compared to the rats in the control group at p<0.05. The total cholesterol (TC), triacylglycerol (TG), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) concentrations in the sera of the rats were determined at the end of the experiment. The result obtained showed that the TC, TG, HDL-C and LDL-C levels increased significantly in the rats of the experimental groups when compared to the rats in the control group at p<0.05. The observation from this study suggests that long-term exposure to Carbamate treated net in household setting may alter the level of the serum lipid profile of the experimental animals, thereby leading to various health complications.

Conjugated linoleic acid suppresses the secretion of atherogenic lipoproteins from human HepG2 liver cells

2005 ◽  
Vol 43 (3) ◽  
Author(s):  
Sebely Pal ◽  
Ryusuke Takechi ◽  
Suleen S. Ho
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Palmitic Acid ◽  
Conjugated Linoleic Acid ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Liver Cells ◽  
The Body ◽  
Low Density

AbstractStudies in healthy humans have shown that consumption of conjugated linoleic acid (CLA) significantly reduced very-low-density lipoprotein (VLDL) and low-density lipoprotein (LDL) blood concentrations. We propose that decreased concentrations are due to the inhibition of VLDL production and secretion [measured by apolipoprotein B100 (apoB100)] from the liver. To investigate the effects of a mixture of CLA isomers on VLDL metabolism, HepG2 liver cells were incubated for 24h with 50μmol/L of the different fatty acids. Effects of CLA were compared to a saturated fatty acid (palmitic acid), an n-6 fatty acid (linoleic acid) and no treatment (control). HepG2-cell apoB100 levels were measured using Western blotting. ApoB100 secretion was significantly decreased in cells treated with CLA (44%, p<0.005) compared to control cells and those enriched with palmitic acid. Treatment of cells with CLA also decreased intracellular cholesterol levels. Collectively, these results demonstrate that CLA reduces apoB100 production and secretion compared to saturated and polyunsaturated fatty acids, possibly by limiting the availability of free cholesterol (required for apoB100 production). A reduction in apoB100 production in the body would decrease the levels of VLDL and atherogenic LDL and thus reduce the risk of developing cardiovascular disease.

Sign in / Sign up

Export Citation Format

Share Document