Using platelet-rich plasma to treat hair loss and thinning

2020 ◽  
Vol 9 (8) ◽  
pp. 326-332
Author(s):  
Daniel Sister
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Hair Loss ◽  
Real Option ◽  
Platelet Rich Plasma ◽  
Men And Women ◽  
Technological Advances ◽  
Long Time ◽  
Chemical Products ◽  
Folk Remedies

Hair loss and thinning have always been a huge concern for men and women across the globe. Numerous treatments have been tried and tested, ranging rom folk remedies to chemical products. Unfortunately, none have proven a regular efficacy, without mentioning the possible side effects. So, for a long time, the only real option was hair graft. Now, with technological advances in both growth factors and stem cells, we have a possible solution that is efficient, safe and reproductible: platelet-rich plasma.

Using platelet-rich plasma to treat hair loss and thinning

2020 ◽  
Vol 9 (10) ◽  
pp. 420-426
Author(s):  
Daniel Sister
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Hair Loss ◽  
Real Option ◽  
Platelet Rich Plasma ◽  
Men And Women ◽  
Technological Advances ◽  
Long Time ◽  
Chemical Products ◽  
Folk Remedies

Hair loss and thinning have always been a huge concern for men and women across the globe. Numerous treatments have been tried and tested, ranging rom folk remedies to chemical products. Unfortunately, none have proven a regular efficacy, without mentioning the possible side effects. So, for a long time, the only real option was hair graft. Now, with technological advances in both growth factors and stem cells, we have a possible solution that is efficient, safe and reproducible: platelet-rich plasma.

scholarly journals Effectiveness of Biologic Factors in Shoulder Disorders

2017 ◽  
Vol 11 (1) ◽  
pp. 163-182 ◽  
Author(s):  
Dimitrios Giotis ◽  
Ashkan Aryaei ◽  
Theofanis Vasilakakos ◽  
Nikolaos K. Paschos
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Clinical Studies ◽  
Platelet Rich Plasma ◽  
Repair Process ◽  
Poor Quality ◽  
Loss Of Function ◽  
Shoulder Disorders ◽  
Biologic Factors

Background:Shoulder pathology can cause significant pain, discomfort, and loss of function that all interfere with activities of daily living and may lead to poor quality of life. Primary osteoarthritis and rotator cuff diseases with its sequalae are the main culprits. Management of shoulder disorders using biological factors gained an increasing interest over the last years. This interest reveals the need of effective treatments for shoulder degenerative disorders, and highlights the importance of a comprehensive and detailed understanding of the rapidly increasing knowledge in the field.Methods:This study will describe most of the available biology-based strategies that have been recently developed, focusing on their effectiveness in animal and clinical studies.Results:Data fromin vitrowork will also be briefly presented; in order to further elucidate newly acquired knowledge regarding mechanisms of tissue degeneration and repair that would probably drive translational work in the next decade. The role of platelet rich-plasma, growth factors, stem cells and other alternative treatments will be described in an evidence-based approach, in an attempt to provide guidelines for their clinical application. Finally, certain challenges that biologic treatments face today will be described as an initiative for future strategies.Conclusion:The application of different growth factors and mesenchymal stem cells appears as promising approaches for enhancing biologic repair. However, data from clinical studies are still limited, and future studies need to improve understanding of the repair process in cellular and molecular level and evaluate the effectiveness of biologic factors in the management of shoulder disorders.

scholarly journals Plasma Rich in Growth Factors Induces Cell Proliferation, Migration, Differentiation, and Cell Survival of Adipose-Derived Stem Cells

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Maravillas Mellado-López ◽  
Richard J. Griffeth ◽  
Jose Meseguer-Ripolles ◽  
Ramón Cugat ◽  
Montserrat García ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Stem Cells ◽  
Growth Factors ◽  
Cell Differentiation ◽  
Cell Survival ◽  
Platelet Rich Plasma ◽  
Adipose Derived Stem Cells ◽  
Translational Model ◽  
Tissue Integration ◽  
Serum Substitute

Adipose-derived stem cells (ASCs) are a promising therapeutic alternative for tissue repair in various clinical applications. However, restrictive cell survival, differential tissue integration, and undirected cell differentiation after transplantation in a hostile microenvironment are complications that require refinement. Plasma rich in growth factors (PRGF) from platelet-rich plasma favors human and canine ASC survival, proliferation, and delaying human ASC senescence and autophagocytosis in comparison with serum-containing cultures. In addition, canine and human-derived ASCs efficiently differentiate into osteocytes, adipocytes, or chondrocytes in the presence of PRGF. PRGF treatment induces phosphorylation of AKT preventing ASC death induced by lethal concentrations of hydrogen peroxide. Indeed, AKT inhibition abolished the PRGF apoptosis prevention in ASC exposed to 100 μM of hydrogen peroxide. Here, we show that canine ASCs respond to PRGF stimulus similarly to the human cells regarding cell survival and differentiation postulating the use of dogs as a suitable translational model. Overall, PRGF would be employed as a serum substitute for mesenchymal stem cell amplification to improve cell differentiation and as a preconditioning agent to prevent oxidative cell death.

scholarly journals Role of White Blood Cells in Blood- and Bone Marrow-Based Autologous Therapies

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
William King ◽  
Krista Toler ◽  
Jennifer Woodell-May
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Growth Factors ◽  
Blood Cells ◽  
Platelet Rich Plasma ◽  
White Blood Cells ◽  
Complex Mixture ◽  
Negative Results

There has been significant debate over the role of white blood cells (WBCs) in autologous therapies, with several groups suggesting that WBCs are purely inflammatory. Misconceptions in the practice of biologic orthopedics result in the simplified principle that platelets deliver growth factors, WBCs cause inflammation, and the singular value of bone marrow is the stem cells. The aim of this review is to address these common misconceptions which will enable better development of future orthopedic medical devices. WBC behavior is adaptive in nature and, depending on their environment, WBCs can hinder or induce healing. Successful tissue repair occurs when platelets arrive at a wound site, degranulate, and release growth factors and cytokines which, in turn, recruit WBCs to the damaged tissue. Therefore, a key role of even pure platelet-rich plasma is to recruit WBCs to a wound. Bone marrow contains a complex mixture of vascular cells, white blood cells present at much greater concentrations than in blood, and a small number of progenitor cells and stem cells. The negative results observed for WBC-containing autologous therapies in vitro have not translated to human clinical studies. With an enhanced understanding of the complex WBC biology, the next generation of biologics will be more specific, likely resulting in improved effectiveness.

scholarly journals Current Biological Strategies to Enhance Surgical Treatment for Rotator Cuff Repair

2021 ◽  
Vol 9 ◽  
Author(s):  
Cheng Zhang ◽  
Jun Wu ◽  
Xiang Li ◽  
Zejin Wang ◽  
Weijia William Lu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Rotator Cuff ◽  
Rotator Cuff Tear ◽  
Rotator Cuff Repair ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Cuff Tear ◽  
Research Perspectives ◽  
Cuff Repair

Rotator cuff tear is one of the most common shoulder problems encountered by orthopedic surgeons. Due to the slow healing process and high retear rate, rotator cuff tear has distressed millions of people all around the world every year, especially for the elderly and active athletes. This disease significantly impairs patients’ motor ability and reduces their quality of life. Besides conservative treatment, open and arthroscopic surgery contributes a lot to accelerate the healing process of rotator cuff tear. Currently, there are many emerging novel treatment methods to promote rotator cuff repair. A variety of biological stimulus has been utilized in clinical practice. Among them, platelet-rich plasma, growth factors, stem cells, and exosomes are the most popular biologics in laboratory research and clinical trials. This review will focus on the biologics of bioaugmentation methods for rotator cuff repair and tendon healing, including platelet-rich plasma, growth factors, exosomes and stem cells, etc. Relevant studies are summarized in this review and future research perspectives are introduced.

Sustained Delivery of BMP-2 and Platelet-rich Plasma-released Growth Factors Contributes to Osteogenesis of Human Adipose-derived Stem Cells

Orthopedics ◽  
2012 ◽  
Vol 35 (9) ◽  
pp. e1402-e1409 ◽  
Author(s):  
Linwei Chen ◽  
Xiaolang Lu ◽  
Shi Li ◽  
Qizhi Sun ◽  
Wanli Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Platelet Rich Plasma ◽  
Adipose Derived Stem Cells ◽  
Sustained Delivery

scholarly journals Are Applied Growth Factors Able to Mimic the Positive Effects of Mesenchymal Stem Cells on the Regeneration of Meniscus in the Avascular Zone?

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Johannes Zellner ◽  
Christian Dirk Taeger ◽  
Markus Schaffer ◽  
J. Camilo Roldan ◽  
Markus Loibl ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factors ◽  
Platelet Rich Plasma ◽  
Rabbit Model ◽  
Bioactive Substances ◽  
Positive Effects ◽  
Defect Site ◽  
Avascular Zone

Meniscal lesions in the avascular zone are still a problem in traumatology. Tissue Engineering approaches with mesenchymal stem cells (MSCs) showed successful regeneration of meniscal defects in the avascular zone. However, in daily clinical practice, a single stage regenerative treatment would be preferable for meniscus injuries. In particular, clinically applicable bioactive substances or isolated growth factors like platelet-rich plasma (PRP) or bone morphogenic protein 7 (BMP7) are in the focus of interest. In this study, the effects of PRP and BMP7 on the regeneration of avascular meniscal defects were evaluated. In vitro analysis showed that PRP secretes multiple growth factors over a period of 8 days. BMP7 enhances the collagen II deposition in an aggregate culture model of MSCs. However applied to meniscal defects PRP or BMP7 in combination with a hyaluronan collagen composite matrix failed to significantly improve meniscus healing in the avascular zone in a rabbit model after 3 months. Further information of the repair mechanism at the defect site is needed to develop special release systems or carriers for the appropriate application of growth factors to support biological augmentation of meniscus regeneration.

scholarly journals Platelet-Rich Plasma and Micrografts Enriched with Autologous Human Follicle Mesenchymal Stem Cells Improve Hair Re-Growth in Androgenetic Alopecia. Biomolecular Pathway Analysis and Clinical Evaluation

Biomedicines ◽  
2019 ◽  
Vol 7 (2) ◽  
pp. 27 ◽  
Author(s):  
Pietro Gentile ◽  
Maria G. Scioli ◽  
Alessandra Bielli ◽  
Barbara De Angelis ◽  
Ciro De Sio ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factors ◽  
Hair Growth ◽  
Cellular Proliferation ◽  
Platelet Rich Plasma ◽  
Androgenetic Alopecia ◽  
Dermal Papilla Cells ◽  
Molecular Pathway ◽  
Baseline Values

Platelet rich plasma (PRP) and Micrografts containing human follicle mesenchymal stem cells (HF-MSCs) were tried as a potential treatment for androgenetic alopecia (AGA). However, little to no work has yet to be seen wherein the bio-molecular pathway of HF-MSCs or PRP treatments were analyzed. The aims of this work are to report the clinical effectiveness of HF-MSCs and platelet-rich plasma evaluating and reviewing the most updated information related to the bio-molecular pathway. Twenty-one patients were treated with HF-MSCs injections and 57 patients were treated with A-PRP. The Wnt pathway and Platelet derived-growth factors effects were analyzed. 23 weeks after the last treatment with mean hair thickness increments (29 ± 5.0%) over baseline values for the targeted area. 12 weeks after the last injection with A-PRP mean hair count and hair density (31 ± 2%) increases significantly over baseline values. The increment of Wnt signaling in Dermal Papilla Cells evidently is one of the principal factors that enhances hair growth. Signaling from mesenchymal stem cells and platelet derived growth factors positively influences hair growth through cellular proliferation to prolong the anagen phase (FGF-7), inducing cell growth (ERK activation), stimulating hair follicle development (β-catenin), and suppressing apoptotic cues (Bcl-2 release and Akt activation).

scholarly journals Effect of Activated Platelet-Rich Plasma on Chondrogenic Differentiation of Rabbit Bone Marrow-Derived Mesenchymal Stem Cells

2021 ◽  
Author(s):  
Zhen Wang ◽  
Zheng Wang ◽  
Bin Zhang ◽  
Qinghua Zhao ◽  
Yubao Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Growth Factors ◽  
Chondrogenic Differentiation ◽  
Platelet Rich Plasma ◽  
Cell Counting ◽  
Chondrogenic Medium ◽  
Tissue Repair And Regeneration ◽  
Rabbit Bone

Abstract Background Platelet-rich plasma (PRP) has revealed benefits in tissue repair and regeneration, however, the effect of PRP on proliferation and chondrogenesis of mesenchymal stem cells remains controversial.This study is to evaluate the effect of different concentration PRP on proliferation and chondrogenic differentiation of rabbit bone marrow-derived mesenchymal stem cells(BMSCs). Methods PRP was obtained by centrifugation and activation, in which growth factors and cytokines were detected. BMSCs were isolated from rabbit bone marrow and characterized by flow cytometry. About 5 × 103 BMSCs were cultured in high glucose Dulbecco’s modified Eagle’s medium (HG-DMEM) with 4 different compositions: 10% fetal bovine serum, 5%PRP, 10%PRP, and 15%PRP for consecutive 7 days. Cell counting assays were performed on days 1, 3, 5, and 7 to evaluate the BMSCs proliferation. In chondrogenic differentiation, high-density cell pellets composed of 5 × 105 BMSCs were induced in 4 conditions: commercial chondrogenic medium (control), 5%PRP (HG-DMEM + 5%PRP), 10%PRP (HG-DMEM + 10%PRP), and 15%PRP (HG-DMEM + 15%PRP) for 21 days. The gene expression levels of aggrecan (ACAN), collagen type Ⅱ Alpha 1 (COL2A1), and SRY-Box Transcription Factor 9 (SOX9) in pellets were detected. Histological assessments were performed by morphologically observation and pathological stain. Independent-samples t-test and one-way analysis of variance were used in statistical analyses. Results The concentrations of growth factors and cytokines were elevated in PRP. BMSCs proliferation was enhanced in all groups, and 10% PRP revealed more obvious outcome than the others from day 5. In chondrogenic differentiation, the levels of ACAN, COL2A1, and SOX9 were lower in 3 PRP groups than control, but ACAN and SOX9 was higher in 10% PRP group than 5% and 15%. Histological examinations showed 10% PRP-treated pellets showed more regular appearance, larger size, and abundant extracellular matrix than 5% and 10% groups, but still inferior to commercial chondrogenic medium. Conclusions PRP may enhance the proliferation of rabbit BMSCs, while with limited effect on chondrogenic differentiation compared with commercial chondrogenic medium in pellets culture. Whether optimizing and modifying PRP components would lead to satisfying chondrogenesis of BMSCs, it is deserved furthermore study.

Sign in / Sign up

Export Citation Format

Share Document