scholarly journals Pollen Morphology in Various Life-form of Aquatic Macrophytes

2021 ◽  
Vol 20 (3) ◽  
Author(s):  
Ratchaneekorn Sangsuk ◽  
Henrik Baslev ◽  
Arunothai Jampeetong
Keyword(s):  
Aquatic Plants ◽  
Pollen Morphology ◽  
Aquatic Macrophytes ◽  
Pollen Dispersal ◽  
Pollen Grains ◽  
Life Forms ◽  
Glass Slides ◽  
Exine Ornamentation ◽  
Related Plant ◽  
Inaperturate Pollen

Ability of angiosperms to produce flowers and seeds for the sexual reproduction is important also in aquatic plants. Pollination in aquatic plants is facilitated by insects, wind, and water, however, pollen morphology related to the plant’s life forms and pollen dispersal are not well described. This study investigates pollen morphology of selected aquatic macrophytes. Plants were collected and preserved as dried specimens. Mature pollen grains of each species were separated from the anthers and then placed on glass slides and mounted with distilled water. Pollen shape and size were observed under a light microscope (LM). Number of apertures and exine ornamentation were examined using scanning electron microscope (SEM). Closely related plant species had similar pollen morphology. Among the 28 species studied, pollen size varied from small to very large (range 10–200 µm) and their shapes were prolate-spheroidal, prolate, oblate, suboblate, and oblate-spheroidal. Some species had inaperturate pollen grains; the remainders were monoaperture, triaperture or polyaperture. Both colpate and porate apertures were found. The pollen surfaces were echinate, fovelate, granulate, lophate, plicate, regulate, regulate-fovelate, reticulate, striate, and verrucate, respectively. Keywords: Aquatic plants, Palynology, Pollen ornamentation, Pollination, SEM

scholarly journals Sharing the Agrarian Knowledge with Archaeology: First Evidence of the Dimorphism of Vitis Pollen from the Middle Bronze Age of N Italy (Terramara Santa Rosa di Poviglio)

2021 ◽  
Vol 13 (4) ◽  
pp. 2287
Author(s):  
Anna Maria Mercuri ◽  
Paola Torri ◽  
Assunta Florenzano ◽  
Eleonora Clò ◽  
Marta Mariotti Lippi ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Bronze Age ◽  
Pollen Morphology ◽  
Pollen Dispersal ◽  
Archaeological Site ◽  
Central Mediterranean ◽  
Pollen Dimorphism ◽  
History Of ◽  
Inaperturate Pollen ◽  
Female Flowers

The recovery of inaperturate pollen from functionally female flowers in archaeological layers opens the question of a possible pollen-based discrimination between wild and domesticated Vitis vinifera in prehistoric times. Pollen analysis applied to archaeology has not routinely considered the existence of pollen dimorphism in Vitis, a well-known trait in the field of agrarian studies. Therefore, the inaperturate shape of grapevine pollen is ignored by studies on the archaeobotanical history of viticulture. In this paper we investigate pollen morphology of the domesticated and wild subspecies of V. vinifera, and report the first evidence of inaperturate Vitis pollen from an archaeological site. We studied exemplar cases of plants with hermaphroditic flowers, belonging to the subspecies vinifera with fully developed male and female organs, cases of dioecious plants with male or female flowers, belonging to the wild subspecies sylvestris and cases of V. vinifera subsp. vinifera with morphologically hermaphroditic but functionally female flowers. The pollen produced by hermaphroditic and male flowers is usually trizonocolporate; the pollen produced by female flowers is inaperturate. This paper reports on the inaperturate pollen of Vitis found in an archeological site of the Po Plain, Northern Italy. The site dated to the Bronze Age, which is known to have been a critical age for the use of this plant with a transition from wild to domesticated Vitis in central Mediterranean. Can the inaperturate Vitis pollen be a marker of wild Vitis vinifera in prehistoric times? Palynology suggests a possible new investigation strategy on the ancient history of the wild and cultivated grapevine. The pollen dimorphism also implies a different production and dispersal of pollen of the wild and the domesticated subspecies. Grapevine plants are palynologically different from the other Mediterranean “cultural trees”. In fact, Olea, Juglans and Castanea, which are included in the OJC index, have the same pollen morphology and the same pollen dispersal, in wild and domesticated plants. In contrast, the signal of Vitis pollen in past records may be different depending on the hermaphroditic or dioecious subspecies.

scholarly journals Pollen characters as taxonomic evidence in some species of Dipsacaceae from Iran

2017 ◽  
Vol 24 (2) ◽  
pp. 129-136 ◽  
Author(s):  
Ebadi-Nahari Mostafa ◽  
Nikzat-Siahkolaee Sedigheh ◽  
Eftekharian Rosa
Keyword(s):  
Pollen Morphology ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Pollen Type ◽  
Upgma Tree ◽  
Pollen Characters ◽  
Exine Ornamentation ◽  
The Family ◽  
The World ◽  
Plant Taxon

Pollen morphology of nine species representing four genera: Cephalaria Schrad, Dipsacus L., Pterocephalus Vaill. and Scabiosa L. of the family Dipsacaceae in Iran has been investigated by means of scanning electron microscopy (SEM). The results showed that pollen grains were triporate and tricolpate. The pollen type of Scabiosa rotata Bieb. (tri- and tetraporate) is the first report in the world. The sizes of pollen grains fall into the classification group magna (pollen grain diameter 50–100 μm). Pollen shapes vary from preoblate to prolate and their polar views were triangulate and lobate. The exine ornamentation varies from gemmate in S. rotata to spinulate in the rest studied species. Species of Scabiosa have been dispersed in UPGMA tree that this confirmed the previous studies about taxonomic problems and species complexity in this genus. These results show the transfer of the some Scabisoa species to Lomelosia Raf. based on palynological characters. Pollen morphology of the family is helpful at the generic and specific level.Bangladesh J. Plant Taxon. 24(2): 129–136, 2017 (December)

scholarly journals Pollen morphology of Iranian Dracocephalum L. (Lamiaceae) and its taxonomic significance

2015 ◽  
Vol 22 (2) ◽  
pp. 99-110 ◽  
Author(s):  
Maryam Naderifar ◽  
Ali Sonboli ◽  
Abbas Gholipour
Keyword(s):  
Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Polar View ◽  
Taxonomic Significance ◽  
Infrageneric Classification ◽  
Exine Ornamentation ◽  
Plant Taxon ◽  
Scanning Electron

Pollen morphology of 11 Iranian Dracocephalum L. species was investigated using light and scanning electron microscopy to evaluate their taxonomic significance for the infrageneric classification of the genus. Pollen grains of all examined taxa were isopolar, hexacolpate, circular in polar view and spheroidal to prolate in equatorial view (P/E = 1.0?2.0). The smallest pollen grains were observed in D. aucheri (P = 29.7 ?m, E = 22.6 ?m), while the largest pollen was found in D. lindbergii (P = 45.1 ?m, E = 33.7 ?m). The highest and lowest apocolpium index (AI) were measured in D. aucheri (AI = 0.27) and D. surmandinum (AI = 0.08), respectively. Colpus membrane was egranulate in all examined species except for D. multicaule and D. ghahremanii. The main exine ornamentation type was characterized as bireticulate including five different subtypes. The results revealed that the exine ornamentation is a diagnostic character useful for the classification of Dracocephalum.Bangladesh J. Plant Taxon. 22(2): 99-110, 2015 (December)

scholarly journals Relationship between pollen morphology and chromosome numbers in Brazilian species of Lippia L. (Verbenaceae)

2013 ◽  
Vol 85 (1) ◽  
pp. 147-157 ◽  
Author(s):  
Saulo M. Sousa ◽  
Patrícia M.O. Pierre ◽  
Giovana A. Torres ◽  
Lisete C. Davide ◽  
Lyderson F. Viccini
Keyword(s):  
Chromosome Numbers ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Positive Association ◽  
Single Species ◽  
Morphological Data ◽  
Prolate Spheroidal ◽  
Oblate Spheroidal ◽  
Exine Ornamentation ◽  
Different Types

The pollen morphology and exine structure of 17 species of Lippia L. were investigated in this work using light and scanning electron microscopy. Among the species studied, 14 showed tricolporate pollen grains, two had tri- and tetracolporate pollen grains and a single species exhibited, only tetracolporate pollen. The amb ranged from triangular to square, and the shape varied from oblate-spheroidal to prolate-spheroidal. Three different types of exine ornamentation were observed: psilate, scabrate and perforate. In addition to morphological data, we found positive association between the chromosome numbers and size of pollen grains, and also between the length and width of the colpi. The results indicate that the characteristics of pollen grains in Lippia may be used as an additional taxonomic character of the genus.

scholarly journals Distinguishing Oblacinska sour cherry clones (Prunus cerasus L.) by pollen morphology

Genetika ◽  
2020 ◽  
Vol 52 (1) ◽  
pp. 187-198 ◽  
Author(s):  
Dragan Nikolic ◽  
Dragan Milatovic ◽  
Aleksandar Radovic ◽  
Jugoslav Trajkovic
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Sour Cherry ◽  
Prunus Cerasus ◽  
Morphological Parameters ◽  
Exine Ornamentation ◽  
Pollen Shape ◽  
Scanning Electron

Morphology and ultrastructure of pollen grains were studied in 13 Obla?inska sour cherry clones in three years using scanning electron microscopy (SEM). All studied clones had isopolar, radially symmetric and tricolpate pollen grains. Length and width of pollen grains varied in a range of 50.03-55.97 ?m and 25.16-28.37 ?m respectively. Pollen shape in all the studied clones was identified as prolate. The smallest colpus length was found in the clone 8 (43.73 ?m), and the highest in the clone 3 (49.16 ?m). The highest colpus and mesocolpium width had the clone 3 (1.73 ?m; 14.87 ?m) and the lowest had the clone 7 (1.46 ?m; 13.78 ?m). All studied clones had striate exine ornamentation. Number of ridges per 100 ?m2 of the exine surface was the highest in the clone 11 (16.7) and lowest in the clone 2 (14.1). Ridge and furrow width ranged from 0.46 to 0.61 ?m and from 0.45 to 0.59 ?m, respectively. The clones have been classified into three clusters based on all studied properties. Pollen grains examination by SEM indicated that several morphological parameters (pollen size and exine characteristics) can be used to distinguish Obla?inska sour cherry clones.

Pollen morphology of subfamily Maloideae (Roseaceae) with special focus on the genus Photinia

Phytotaxa ◽  
2019 ◽  
Vol 404 (5) ◽  
pp. 171
Author(s):  
MITRA LAL PATHAK ◽  
MUHAMMAD IDREES ◽  
BO XU ◽  
XIN FEN GAO
Keyword(s):  
Pollen Morphology ◽  
Pollen Grains ◽  
Sensu Stricto ◽  
Pollen Size ◽  
Special Focus ◽  
Type I ◽  
Generic Level ◽  
Oblate Spheroidal ◽  
Exine Ornamentation ◽  
Pollen Shape

Pollen morphology of 27 species of Photinia sensu stricto and five closely related genera of subfamily Maloideae (Rosaceae) were studied in detail. Palynomorphological characteristics were examined using scanning electron microscopy (SEM). Comparative pollen analysis was accomplished based on pollen size, shape, polar and equatorial views, polar and equatorial diameter ratio (P/E ratio), width of aperture, and exine ornamentation. The pollen grains of studied species were found to be monad, tricolporate, small to medium in size (P=17.42–30.45 μm, E=9.26–20.18 μm) and to have shapes oblate-spheroidal (0.88–1.0 μm), prolate-spheroidal (1.01–1.14 μm), sub-prolate (1.15–1.33 μm), prolate (1.34–2 μm), and perprolate (>2 μm). The exine ornamentation was perforated-striate, and two different types were identified. The result of quantitative characters has paltry taxonomic importance. The characters studied here delimit at generic level for some genera but not at species level. The obtained result was partially consistent with that of molecular studies published earlier. The pollen size and shape were found to be different between Asian and American species of Photinia. Especially exine sculpture was found to be an important feature to distinguish species of all genera studied. PCA analysis showed that the pollen shape, pollen sculpture, pollen size, and polarity are the key characters to distinguish the species of Photinia and the closely related genera in subfamily Maloideae. Based on pollen exine, two groups (Type I and Type II) were distinguished. The aim of the present study was to find out the taxonomic significance of palynomorphological characteristics in subfamily Maloideae (Rosaceae).

Pollen morphology of selected species of Anacardiaceae and its taxonomic significance

Rodriguésia ◽  
2021 ◽  
Vol 72 ◽  
Author(s):  
Alano César Rocha de Assis ◽  
Eduardo Custódio Gasparino ◽  
Marileide Dias Saba
Keyword(s):  
Phylogenetic Relationships ◽  
Quantitative Data ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Taxonomic Significance ◽  
Electron Microscopies ◽  
Oblate Spheroidal ◽  
Exine Ornamentation ◽  
The Family ◽  
Scanning Electron

Abstract In the present work, the pollen morphology of 22 species of Anacardiaceae was analysed, occurring in the state of Bahia, Brazil. The palynological material was obtained from specimens deposited in herbaria. The pollen grains were acetolysed, measured, illustrated and described under light and scanning electron microscopies. The quantitative data were statistically analysed according to sample size. The analysed species presented pollen grains in monads, small to medium sized, isopolar, amb circular to subtriangular, with shapes varying from oblate-spheroidal to prolate, 3-colporate, with fastigium in some species. The exine ornamentation was characterized as psilate, striate, microreticulate-striate, striato-perforate, suprastriato-infra(micro)reticulate. The pollen morphology variation confirms the eurypalynous character of the family. The attributes of the apertures and exine may collaborate in the taxonomic circumscription and provide a clarification of the phylogenetic relationships of the group.

Pollen morphology of the coastal species of Turkish Limonium (Plumbaginaceae)

Phytotaxa ◽  
2019 ◽  
Vol 388 (3) ◽  
pp. 212 ◽  
Author(s):  
CAHİT DOĞAN ◽  
EDİBE ÖZMEN BAYSAL
Keyword(s):  
Light Microscope ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Morphological Characters ◽  
Accurate Identification ◽  
Taxonomic Key ◽  
Coastal Species ◽  
Exine Ornamentation ◽  
Saline Habitats ◽  
Scanning Electron

The pollen morphology of nine Limonium species (Plumbaginaceae), occurring along the coastal saline habitats of Turkey, are investigated. Pollen slides were prepared according to the methods of Wodehouse (W) and Erdtman (E) and examined using light microscope. Then pollen grains were placed directly to stubs and covered by gold-palladium mixture for scanning electron analyses. Pollen grains are usually tricolpate, rarely syncolpate, dicolpate, tetracolpate, and pentacolpate. Shape of pollen grains are varing from prolate-sphaeroidal to oblate. Medium to large sized pollen grains of Limonium has long and wide colpi with distinct margins, and acute terminals. Exine structure is semi-tectate and exine ornamentation is microreticulate-microechinate or reticulate-microechinate. Pollen grains of Limonium species have shown variation on colpus type, shape and exine ornamentation. Examined Limonium species can be seperated in three unformal groups according to the palynological features, and their pollen definitions have been done. Besides, a taxonomic key was proposed for quick and accurate identification based on different palyno-morphological characters.

scholarly journals Pollen morphology and variability of Polish native species from genus Salix L.

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0243993
Author(s):  
Irmina Maciejewska-Rutkowska ◽  
Jan Bocianowski ◽  
Dorota Wrońska-Pilarek
Keyword(s):  
Native Species ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Polar Axis ◽  
High Variability ◽  
Interspecific Variability ◽  
Equatorial Diameter ◽  
Exine Ornamentation ◽  
Lower Variability ◽  
Biometric Features

The pollen morphology was studied of 24 Salix species native to Poland, which represented two subgenera, 17 sections and five subsections occurring in Poland. The aim of this study was to discover the taxonomical usefulness of the pollen features under analysis, and to investigate the ranges of their interspecific variability. In total, 720 pollen grains were studied. They were analysed with respect to seven quantitative features (length of the polar axis ˗ P, equatorial diameter ˗ E, length of the ectoaperture ˗ Le, exine thickness ˗ Ex, and P/E, Ex/P and Le/P ratios) and the following qualitative ones: pollen outline and exine ornamentation. The most important features were exine ornamentation (muri, lumina and margo) characters. The pollen features should be treated as auxiliary because they allowed to distinguish eight individual Salix species, and five groups of species. Statistical analysis of the studied traits indicated a high variability among the tested species. The most variable biometric features were P, E and Le, while lower variability occurred in P/E, Le/P and d/E.

scholarly journals Pollen Morphology of Some Turkish Salvia L. (Lamiaceae: Mentheae) Species

2020 ◽  
Vol 38 ◽  
Author(s):  
K. AKTA ◽  
C. ÖZDEMIR ◽  
M. ÖZKAN ◽  
P. BARAN
Keyword(s):  
Pollen Morphology ◽  
Pollen Grains ◽  
Polar Axis ◽  
Radial Symmetry ◽  
Exine Ornamentation ◽  
Pollen Shape ◽  
Axis Length ◽  
Different Characteristics ◽  
Scanning Electron

ABSTRACT: In this presented work, the pollen morphologies of twenty-one taxa, nine of them endemic from Turkey, belonging to the genus Salvia (Lamiaceae: sub-family Nepetoideae: tribe Mentheae: sub-tribe Salviinae), S. aethiopis L., S. argentea L., S. aytachii Vural & Adigüzel, S. blepharochlaena Hedge & Hub.-Mor., S. cadmica Boiss., S. ceratophylla L., S. cryptantha Montbret & Aucher, S. frigida Boiss., S. fructicosa Miller, S. halophile Hedge, S. napifolia Jacq., S. microstegia Boiss. & Bal., S. recognita Fisch. & Mey. S. sclarea L., S. smyrnaea Boiss. S. suffruticosa Montbret & Aucher, S. tchihatcheffii (Fisch. & Mey.) Boiss., S. tometosa Bertol, S. verbenaca L., S. viridis L., and S. wiedemanni Boiss. and collected throughout the Turkey, have been intensively studied by using light (LM) and a scanning electron (SEM) microscopy. The objectives of this study are to investigate the pollen morphologies of twenty-one taxa of Turkish Salvia (nine of which are endemic) and to present similar and different characteristics of the taxa. The investigated whole pollen grains had a structure of both isopolar and radial symmetry. Overall, types of aperture were mostly hexacolpate, but it was also determined that the aperture type of S. viridis was both hexacolpate and octacolpate. The pollen grains had characteristic shapes of spheroidal, suboblate, prolate and subprolate. The exine ornamentation was bireticulate and reticulate-perforate. It was determined by the results of palynological studies that pollen morphology changed amongst these studied taxa. As a conclusion, the results of the present study show that palynological characters such as pollen shape, polar axis length (P), equatorial axis length (E), aperture numbers and types and exine ornamentation, exhibit remarkable differences amongst the studied taxa.

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