Neuroprotective Effect of vitex negundo Against Scopolamine Induced Cognitive Impairment and Oxidative Stress in Wistar albino Rats

Objective: Oxidative stress appears to be an early event involved in the pathogenesis of Alzheimer's disease (AD). The present study was designed to investigate the neuroprotective effects of citrullus lanatus on bilateral common carotid artery occlusion (BCCAO) induced cognitive impairment and oxidative stress in Wistar albino rats. Methods: Cognitive impairment and oxidative stress were induced by BCCAO for 30 min, followed by 7 d reperfusion of male wistar rats. Morris water maze and rectangular maze performance tests and locomotor activity were used to assess memory performance tasks. To study the activity, rats weighing 250-300g were pre-treated with successive extracts of n-hexane fraction (HF), ethyl acetate fraction (EAF), ethanol fraction (EF) and aqueous fraction (AF) of 400 mg/kg, 200 mg/kg, p. o of each for 10 d and the treatment was continued for another 7 d after cerebral ischemia. Various biochemical parameters like lipid peroxidation, Catalase, DPPH and AchE were also estimated in the brain after the treatment. Results: There was significantly increased oxidative stress and cholinesterase activity with cognitive decline in the hippocampus in rats of BCCAO group as compared to sham-operated (p<0.05). The animals treated with Donepezil, HF and EF prevented the biochemical changes significantly (p<0.001) and there was significant (p<0.001) improvement in cognitive parameters compared to BCCAO treated rats. Conclusion: Thus present study indicates the neuroprotective effect of citrulus lanatus seed extract (HF and EF) against BCCAO induced cognitive impairment and associative oxidative damage.

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Neuroprotective effect of methanolic extract of Sargassum wightii on rotenone-induced parkinsonism-like symptoms in Wistar albino rats

Journal of Herbmed Pharmacology ◽

10.34172/jhp.2021.54 ◽

2021 ◽

Vol 10 (4) ◽

pp. 468-475

Author(s):

Sradhasini Rout ◽

Bandana Rath ◽

Subrat Kumar Bhattamisra ◽

Anjan Kumar ◽

Ishani Rath ◽

...

Keyword(s):

Oxidative Stress ◽

Motor Coordination ◽

Methanolic Extract ◽

Neuroprotective Effect ◽

Albino Rats ◽

Dopamine Level ◽

Oxidative Stress Markers ◽

Sargassum Wightii ◽

Stress Markers ◽

Wistar Albino Rats

Introduction: The pathogenesis of Parkinson’s disease (PD) is multifactorial in which oxidative stress, neuroinflammation, and mitochondrial dysfunction are the leading factors. Currently, the antioxidant and anti-inflammatory agents of natural sources as neuroprotectants have raised much attention. The current study aimed to explore the neuroprotective effect of methanolic extract of Sargassum wightii in male Wistar albino rats against rotenone-induced PD. Methods: The rats were administered with rotenone (10 mg/kg orally) daily for 28 days to induce PD. S. wightii (200 mg/kg and 400 mg/kg) and levodopa+carbidopa combination (10 mg/kg) were administered to different groups of rats one hour prior to rotenone for 28 days. Behavioral parameters (akinesia, tremor, motor coordination, and locomotor activities) and body weight were recorded on days 14th and 28th of drug treatment. On the 28th day, the animals were sacrificed for the neurobiochemical analyses of brain tissue. Results: Rotenone treatment caused a significant reduction in behavioural parameters (P < 0.001), neurochemical deficits (P < 0.001), and elevation of oxidative stress markers (P < 0.001) in the brain. Pre-treatment with S. wightii at 200 mg/kg and 400 mg/kg doses significantly attenuated the rotenone-induced behavioral alterations and restored the mitochondrial NADH dehydrogenase activity and dopamine level in the striatum (P < 0.001). Moreover, 400 mg/kg of S. wightii restored the rotenone-induced increased oxidative stress markers like malondialdehyde (MDA), superoxide dismutase (SOD), and reduced glutathione (GSH) in the striatum (P < 0.01). Conclusion: S. wightii has provided a neuroprotective effect, probably by virtue of its antioxidant and dopamine restoring potential. Hence, it may offer a promising and new therapeutic lead for the treatment of PD but needs further research.

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Effects of Safranal on Tissue Oxidative Stress in Sub-Chronic Thinner-Addicted Rats

Journal of the Hellenic Veterinary Medical Society ◽

10.12681/jhvms.22942 ◽

2020 ◽

Vol 71 (1) ◽

pp. 1997

Author(s):

M. DÜZ ◽

A. F. FIDAN

Keyword(s):

Oxidative Stress ◽

Nitric Oxide ◽

Protective Effect ◽

Total Antioxidant Capacity ◽

Reduced Glutathione ◽

Control Group ◽

Albino Rats ◽

Total Antioxidant ◽

Wistar Albino Rats ◽

And Oxidative Stress

The present study was carried out to determine the effects of sub-chronic thinner addiction on the oxidant-antioxidant balance and oxidative stress on certain tissues and the possible protective effect of safranal against thinner toxication in rats. Adult male Wistar albino rats were randomly divided into four groups of 10 animals each as follows: control (C), safranal (S), thinner (T) and thinner+safranal (T+S). The control group received 1cc saline by gastric gavage. Safranal was administered to S and T+S groups by using gastric gavage at a dose of 100 mg/kg/day and volume of 0.1 mL/kg/day. Thinner inhalation was applied to T and T+S groups in a container with NaOH tablets twice a day. Levels of malondialdehyde (MDA), reduced glutathione (GSH), nitric oxide (NOx) metabolites, total antioxidant capacity (TAS) and total oxidant capacity (TOS) were determined in liver, lung, brain, kidney and testis tissues of the rats. In the T+S group, it was observed that the MDA levels significantly decreased in all tissues, except the kidney, in comparison to the thinner inhalation group (p = 0.000). When the NOx levels of the T+S group were compared with the levels of the T group, it was concluded that there existed a statistically significant decrease in the NOx levels in alltissues (p = 0.000). In T+S group, it was observed that safranal either eliminated or mitigated oxidative stress that developed in tissues through decreasing MDA and TOS levels and increasing GSH and TAS levels and caused significant decreases in NOX levels in all tissues. As a result, it was determined that safranal, although not uniform for all tissue types, had a protective potential against the damaging effects of oxidative stress caused by sub-chronic thinner inhalation.

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Neuroprotective effect of Rhodiola rosea Linn against MPTP induced cognitive impairment and oxidative stress

Annals of Neurosciences ◽

10.5214/ans.0972.7531.200204 ◽

2013 ◽

Vol 20 (2) ◽

Cited By ~ 12

Author(s):

Reenu Jacob ◽

G Nalini ◽

N Chidambaranathan

Keyword(s):

Oxidative Stress ◽

Cognitive Impairment ◽

Neuroprotective Effect ◽

Rhodiola Rosea ◽

And Oxidative Stress

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Beneficial effects of quercetin on renal injury and oxidative stress caused by ciprofloxacin in rats

Human & Experimental Toxicology ◽

10.1177/0960327115584686 ◽

2015 ◽

Vol 35 (3) ◽

pp. 276-281 ◽

Cited By ~ 7

Author(s):

H Elbe ◽

Z Dogan ◽

E Taslidere ◽

A Cetin ◽

Y Turkoz

Keyword(s):

Oxidative Stress ◽

Renal Injury ◽

Kidney Tissue ◽

Albino Rats ◽

Therapeutic Effects ◽

Histopathological Changes ◽

Tubular Atrophy ◽

Beneficial Effects ◽

Wistar Albino Rats ◽

And Oxidative Stress

Ciprofloxacin is a broad-spectrum quinolone antibiotic commonly used in clinical practice. Quercetin is an antioxidant belongs to flavonoid group. It inhibits the production of superoxide anion. In this study, we aimed to evaluate the effects of quercetin on renal injury and oxidative stress caused by ciprofloxacin. Twenty-eight female Wistar albino rats were divided into four groups: control, quercetin (20 mg kg−1 day−1 gavage for 21 days), ciprofloxacin (20 mg kg−1 twice a day intraperitoneally for 10 days), and ciprofloxacin + quercetin. Samples were processed for histological and biochemical evaluations. Malondialdehyde (MDA) and glutathione (GSH) levels, superoxide dismutase (SOD), and catalase (CAT) activities were measured in kidney tissue. The ciprofloxacin group showed histopathological changes such as infiltration, dilatation in tubules, tubular atrophy, reduction of Bowman’s space, congestion, hemorrhage, and necrosis. In the ciprofloxacin + quercetin group, these histopathological changes markedly reduced. MDA levels increased in the ciprofloxacin group and decreased in the ciptofloxacin + quercetin group. SOD and CAT activities and GSH levels significantly decreased in the ciprofloxacin group. On the other hand, in the ciprofloxacin + quercetin group, SOD and CAT activities and GSH levels significantly increased with regard to the ciprofloxacin group. We concluded that quercetin has antioxidative and therapeutic effects on renal injury and oxidative stress caused by ciprofloxacin in rats.

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