Anticancer Activity of Graphene Oxide/5-FU on CT26 dsRED Adenocarcinoma Cell Line

Cancer is one of the greatest health challenges in the world. Every year, many people die because of cancer. Chemotherapy is one of the treatment options in cancer disease. Fluorouracil )5-FU( is one of the chemotherapy dr0075gs, but it has relatively low toxic effect on tumor cells when it is used on free form, which also results in its poor efficacy. GO (graphene oxide) has a single-atomic layer and has several functional groups such as epoxide, carbonyl, carboxyl and hydroxyl which makes it a suitable carrier for drug loading. In the present study, we loaded 5-FU on GO nanocarrier to produce GO/5-FU, and characterized it by FT-IR. CT26 Ds-Red adenocarcinoma cell line was treated with GO/5-FU, free 5-FU, GO, and PBS (Phosphate buffer saline). The results showed significant inhibition of the CT26 Ds-Red cells using GO/5-FU compared to free 5-FU (P<0.05). Therefore, loaded 5-FU on GO (GO/5-FU) could be a new approach for optimization of 5-FU tumor cytotoxicity.

Download Full-text

Effects of growth factors EGF and TGF-β in the endometrial adenocarcinoma cell line HEC 1B

Acta Endocrinologica ◽

10.1530/acta.0.120s236 ◽

1989 ◽

Vol 120 (3_Suppl) ◽

pp. S236 ◽

Cited By ~ 2

Author(s):

T. STROWITZKI ◽

M. WIMBAUER ◽

D. PFEIFFER ◽

P. SCHEIDEL

Keyword(s):

Growth Factors ◽

Cell Line ◽

Endometrial Adenocarcinoma ◽

Adenocarcinoma Cell Line ◽

Adenocarcinoma Cell

Download Full-text

Synthesis, Anti-proliferative Evaluation, and Molecular Docking Studies of 3-(alkylthio)-5,6-diaryl-1,2,4-triazines as Tubulin Polymerization Inhibitors

Letters in Drug Design & Discovery ◽

10.2174/1570180815666180727114216 ◽

2019 ◽

Vol 16 (11) ◽

pp. 1194-1201 ◽

Cited By ~ 3

Author(s):

Farhad Saravani ◽

Ebrahim Saeedian Moghadam ◽

Hafezeh Salehabadi ◽

Seyednasser Ostad ◽

Morteza Pirali Hamedani ◽

...

Keyword(s):

Molecular Modeling ◽

Cytotoxic Activity ◽

Binding Site ◽

Cell Line ◽

Cell Lines ◽

Tubulin Polymerization ◽

Adenocarcinoma Cell Line ◽

Adenocarcinoma Cell ◽

Colchicine Binding Site ◽

Anti Cancer

Background: The role of microtubules in cell division and signaling, intercellular transport, and mitosis has been well known. Hence, they have been targeted for several anti-cancer drugs. Methods: A series of 3-(alkylthio)-5,6-diphenyl-1,2,4-triazines were prepared and evaluated for their cytotoxic activities in vitro against three human cancer cell lines; human colon carcinoma cells HT-29, human breast adenocarcinoma cell line MCF-7, human Caucasian gastric adenocarcinoma cell line AGS as well as fibroblast cell line NIH-3T3 by MTT assay. Docking simulation was performed to insert these compounds into the crystal structure of tubulin at the colchicine binding site to determine a probable binding model. Compound 5d as the most active compound was selected for studying of microtubule disruption. Results: Compound 5d showed potent cytotoxic activity against all cell lines. The molecular modeling study revealed that some derivatives of triazine strongly bind to colchicine binding site. The tubulin polymerization assay kit showed that the cytotoxic activity of 5d may be related to inhibition of tubulin polymerization. Conclusion: The cytotoxicity and molecular modeling study of the synthesized compounds with their inhibition activity in tubulin polymerization demonstrate the potential of triazine derivatives for development of new anti-cancer agents.

Download Full-text

Selective cytotoxicity of vanadium complexes on human pancreatic ductal adenocarcinoma cell line by inducing necroptosis, apoptosis and mitotic catastrophe process

Oncotarget ◽

10.18632/oncotarget.19454 ◽

2017 ◽

Vol 8 (36) ◽

pp. 60324-60341 ◽

Cited By ~ 19

Author(s):

Szymon Kowalski ◽

Stanisław Hać ◽

Dariusz Wyrzykowski ◽

Agata Zauszkiewicz-Pawlak ◽

Iwona Inkielewicz-Stępniak

Keyword(s):

Pancreatic Ductal Adenocarcinoma ◽

Cell Line ◽

Mitotic Catastrophe ◽

Ductal Adenocarcinoma ◽

Vanadium Complexes ◽

Adenocarcinoma Cell Line ◽

Selective Cytotoxicity ◽

Adenocarcinoma Cell

Download Full-text

Chemical Profiling, Antioxidant, Antiproliferative, and Antibacterial Potentials of Chemically Characterized Extract of Citrullus colocynthis L. Seeds

Separations ◽

10.3390/separations8080114 ◽

2021 ◽

Vol 8 (8) ◽

pp. 114

Author(s):

Mohammed Bourhia ◽

Kaoutar Bouothmany ◽

Hanane Bakrim ◽

Safa Hadrach ◽

Ahmad Mohammad Salamatullah ◽

...

Keyword(s):

Chemical Composition ◽

Cell Line ◽

Antiproliferative Activity ◽

Seed Extract ◽

Diffusion Method ◽

Citrullus Colocynthis ◽

Adenocarcinoma Cell Line ◽

Adenocarcinoma Cell ◽

Ht 29 ◽

Β Carotene

Background: Citrullus colocynthis L. (C. colocynthis) is commonly known as colocynth. It belongs to the family Cucurbitaceae that is frequently used in alternative medicine in the north of Africa. The aim of the study: the present research was undertaken to investigate the chemical composition, antioxidant, antiproliferative, and antibacterial potentials of C. colocynthis seed extract. Material and methods: the chemical composition of C. colocynthis seed organic extract was characterized using gas chromatography/mass spectrometry (GC-MS). The antioxidant property was carried out using both β-carotene bleaching and DPPH assays. The antibacterial effect was effectuated using the agar disc diffusion method. The antiproliferative activity vs. human colorectal adenocarcinoma cell line (HT-29) and human breast adenocarcinoma cell line (MDA MB 231) were carried by WST-1 test. The chemical analysis showed the presence of interesting potentially bioactive compounds. The studied plant extract exhibited antioxidant potential with IC50 value of 2. 22 mg/mL (β-carotene bleaching) and 8.98 ± 0.619 mg/mL (DPPH). Concerning the antiproliferative activity, the seed extract was effective in MDA-MB-231 and HT-29 cancer cells with IC50 values 86.89 ± 3.395 and 242.1 ± 17.9 μg/mL, respectively, whilst the extract of Citrullus colocynthis seeds was non-toxic in healthy human dermal fibroblasts. Regarding the antibacterial test, the extract was effective in Gram-positive bacteria only. Conclusion: The outcome of this research indicated that the extracts from C. colocynthis seeds may compose a promising source with interesting compounds that can be used to fight cancer, free radicals damage, and bacterial infections.

Download Full-text