Future Clinical Application of β-galactosidase Stabilized by Magnesium oxide Nanoparticles

The present study demonstrates the application of freshly prepared neem leaf extract as a reducing agent for synthesizing magnesium oxide nanoparticles (MgO-NPs). In silico interaction of Aspergillus oryzae β-galactosidase with MgO-NPs was observed by using molecular docking program Dock v.6.5 while the visual analyses and illustration of protein–ligand complex were investigated by utilizing chimera v.1.6.2 and PyMOL v.1.3 softwares. The prepared nanomatrix provided 83% immobilization yield, and broadened the biocatalytic activity of immobilized β-galactosidase at higher pH and temperature ranges. Immobilized β-galactosidase exhibited greater activity even at 5.0% galactose concentration as compared to the soluble enzyme under similar experimental conditions. Hence, the use of green nanotechnology makes the process inexpensive, and therefore, immobilization of these enzymes on such nanoparticles can help to recover the enzyme, which ultimately decreases the cost of process.

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The catalytic ozonation of diazinon using nano-MgO@CNT@Gr as a new heterogenous catalyst: the optimization of effective factors by response surface methodology

RSC Advances ◽

10.1039/c9ra10095d ◽

2020 ◽

Vol 10 (13) ◽

pp. 7718-7731 ◽

Cited By ~ 4

Author(s):

Ghorban Asgari ◽

Abdolmotaleb Seidmohammadi ◽

Ali Esrafili ◽

Javad Faradmal ◽

Mohammad Noori Sepehr ◽

...

Keyword(s):

Carbon Nanotubes ◽

Response Surface Methodology ◽

Magnesium Oxide ◽

Catalytic Ozonation ◽

Oxide Nanoparticles ◽

Hybrid Catalyst ◽

Experimental Conditions ◽

Effective Factors ◽

Magnesium Oxide Nanoparticles ◽

Heterogenous Catalyst

In this research, the degradation of the insecticide diazinon was studied using a new hybrid catalyst consisting of magnesium oxide nanoparticles (nano-MgO), carbon nanotubes (CNTs), and graphite (Gr), nano-MgO@CNT@Gr, under various experimental conditions.

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Antibacterial activity of magnesium oxide nanoparticles prepared by Calcination method

International Journal of Pharmaceutical Quality Assurance ◽

10.25258/ijpqa.10.3.25 ◽

2019 ◽

Vol 10 (03) ◽

Author(s):

Elaf Ayad Kadhem ◽

Miaad Hamzah Zghair ◽

Sarah , Hussam H. Tizkam, Shoeb Alahmad Salih Mahdi ◽

Hussam H. Tizkam ◽

Shoeb Alahmad

Keyword(s):

Antibacterial Activity ◽

Magnesium Oxide ◽

Diffusion Method ◽

Oxide Nanoparticles ◽

Gram Negative Bacteria ◽

Gram Positive ◽

Gram Negative ◽

Gram Positive Bacteria ◽

Magnesium Oxide Nanoparticles ◽

Agar Disc

magnesium oxide nanoparticles (MgO NPs) were prepared by simple wet chemical method using different calcination temperatures. The prepared NPs were characterized by Electrostatic Discharge (ESD), Scanning Electron Microscope (SEM) and X-ray Diffraction (XRD). It demonstrates sharp intensive peak with the increase of crystallinty and increase of the size with varying morphologies with respect to increase of calcination temperature. Antibacterial studies were done on gram negative bacteria (E.coli) and gram positive bacteria (S.aureus) by agar disc diffusion method. The zones of inhibitions were found larger for gram positive bacteria than gram negative bacteria, this mean, antibacterial MgO NPs activity more active on gram positive bacteria than gram negative bacteria because of the structural differences. It was found that antibacterial activity of MgO NPs was found it has directly proportional with their concentration.

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Applications of ZnO and MgO Nanoparticles in Reducing Zinc Pollution Level in Rubber Manufacturing Processes: A Review

Current Biochemical Engineering ◽

10.2174/2212711906666200224105931 ◽

2020 ◽

Vol 6 (2) ◽

pp. 103-107 ◽

Cited By ~ 1

Author(s):

Seyyed Mohammad Javadi

Keyword(s):

Zinc Oxide ◽

Fatty Acid ◽

Magnesium Oxide ◽

Environmental Effects ◽

Pollution Level ◽

Oxide Nanoparticles ◽

Mgo Nanoparticles ◽

Magnesium Oxide Nanoparticles ◽

Council Directive ◽

Zno Particles

Background: Rubber vulcanization is a consolidated chemical process to enhance the mechanical properties of the polymeric material by sulfur crosslinking of the polymer chains, such as rubber. Vulcanization Activators are important rubber processing additives that activate sulfur cure and improve the efficiency of sulfur-based cure systems. The most common activator is zinc fatty acid ester that is often formed in-situ by the reaction of fatty acid with zinc oxide. Although zinc is one of the less harmful heavy metals, according to European Council Directive 2004/73/EC, the reduction of zinc level in the environment has become an important task because of its toxic effect on aquatic organisms. : The current study reviews the research achievements in the field of reducing the consumption of micronutrients of ZnO particles based on the use of nanoparticles instead of them in the polymer industry. Among the proposed methods, due to the less environmental effects of magnesium oxide, the use of MgO nanoparticles instead of zinc oxide has also achieved good results. Objective: The aim of this paper is considering suggested different methods on the reduction of using ZnO particles in related industries, the use of ZnO nanoparticles has had better results than its particles. In addition, due to the less environmental effects of magnesium oxide, magnesium oxide nanoparticles can be used instead of micronutrients of zinc oxide. Overall, the results of various investigations show that reducing the diameter of the zinc oxide particles reduces the amount required for curing the rubber and thus reduces its toxic effects. Also, the use of magnesium oxide nanoparticles instead of zinc oxide in different concentrations is investigated.

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An insight into the Mechanism of Antibacterial activity by Magnesium oxide nanoparticles

Journal of Materials Chemistry B ◽

10.1039/d1tb00875g ◽

2021 ◽

Author(s):

Proma Bhattacharya ◽

Aishee Dey ◽

Sudarsan Neogi

Keyword(s):

Antibacterial Activity ◽

Magnesium Oxide ◽

Oxide Nanoparticles ◽

Antibacterial Efficacy ◽

Magnesium Oxide Nanoparticles ◽

Insight Into

The exact mechanism behind the antibacterial efficacy of nanoparticles remain unexplored till date. This study is aimed at shedding light on the mechanism adopted by magnesium oxide nanoparticles prepared in...

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