EFFECT OF UNIONIZED VOLATILE ACID ON ACIDOGENIC PERFORMANCE IN ANAEROBIC SOLID-STATE ORGANIC WASTE DEGRADATION

A research on screening and isolation of cellulolytic bacteria from the gut of larvae Black Soldier treated rice straw feed has been conducted. The purpose of this study is to get the type of cellulolytic bacteria from the gut of larvae and bacteria that have the highest potential to degrade cellulose. Screening and isolation method applied by using intestinal larvae obtained from larval gut vortex at a speed of 1500 rpm. Furthermore, dilution graded from 1 to 10 and grown in media CMC (carboxyl methyl cellulose) at 37 0C and incubated for 48 hours. Observations were made based on the characteristics of the microscopic, macroscopic, biochemical test, cellulolytic activity and the activity of cellulase enzymes selected bacteria. The results showed a 9 cellulolytic bacteria from the gut of the larvae. Bacillus sp. is a bacteria that have the highest potential with cellulolitic activity 2.1 mm (dz/dk), the exponential phase of hour at the 24th, and cellulase enzyme activity of 0.4 U/mL at pH 7 and 0.41 U/mL at pH 8.This research showed that the Black Soldier Flays Larvae (Hermetia illucens) have competencein organic waste degradation, because in Black Soldier Flays Larvae’s gut, cellulolitic enzyme is produced by cellulolitic bacteria, specially Bacillus sp,How to CiteSupriyatna, A., & Ukit, U. (2016). Screening and Isolation of Cellulolytic Bacteria from Gut of Black Soldier Flays Larvae (Hermetia illucens) Feeding with Rice Straw. Biosaintifika: Journal of Biology & Biology Education, 8(3), 314-320.

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Bacterial Succession during Vermicomposting of Silver Wattle (Acacia dealbata Link)

Microorganisms ◽

10.3390/microorganisms10010065 ◽

2021 ◽

Vol 10 (1) ◽

pp. 65

Author(s):

Daniela Rosado ◽

Marcos Pérez-Losada ◽

Manuel Aira ◽

Jorge Domínguez

Keyword(s):

Salicylic Acid ◽

Organic Waste ◽

Sustainable Use ◽

Sequence Variants ◽

Eisenia Andrei ◽

Acacia Dealbata ◽

Β Diversity ◽

Bacterial Succession ◽

Waste Degradation ◽

Functional Profiles

Vermicomposting is the process of organic waste degradation through interactions between earthworms and microbes. A variety of organic wastes can be vermicomposted, producing a nutrient-rich final product that can be used as a soil biofertilizer. Giving the prolific invasive nature of the Australian silver wattle Acacia dealbata Link in Europe, it is important to find alternatives for its sustainable use. However, optimization of vermicomposting needs further comprehension of the fundamental microbial processes. Here, we characterized bacterial succession during the vermicomposting of silver wattle during 56 days using the earthworm species Eisenia andrei. We observed significant differences in α- and β-diversity between fresh silver wattle (day 0) and days 14 and 28, while the bacterial community seemed more stable between days 28 and 56. Accordingly, during the first 28 days, a higher number of taxa experienced significant changes in relative abundance. A microbiome core composed of 10 amplicon sequence variants was identified during the vermicomposting of silver wattle (days 14 to 56). Finally, predicted functional profiles of genes involved in cellulose metabolism, nitrification, and salicylic acid also changed significantly during vermicomposting. This study, hence, provides detailed insights of the bacterial succession occurring during vermicomposting of the silver wattle and the characteristics of its final product as a sustainable plant biofertilizer.

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Isolation of Cellulose Degradation Bacteria (CDB) from acid soil as a potential candidate of organic waste degradation

JERAMI Indonesian Journal of Crop Science ◽

10.25077/jijcs.1.2.26-35.2019 ◽

2019 ◽

Vol 1 (2) ◽

pp. 26-35

Author(s):

Eri Samah

Keyword(s):

Bovine Serum Albumin ◽

Protein Content ◽

Growth Curve ◽

Organic Waste ◽

Cellulose Degradation ◽

Acid Soil ◽

Maximum Activity ◽

Potential Candidate ◽

Clear Zone ◽

Waste Degradation

Background: The study aimed to obtain CDB with high degraded activities, determined growth curve, protein content, and cellulase maximum activity (exoglucanase and endoglucanase). Method: The cellulose activity calculated according to Miller (1959), protein content was measured by Bradford method with bovine serum albumin (BSA) as a standardize protein. Result: Six isolates of CDB were found as potential degradation of organic waste (Km25, Sr75, Jm, U6, G8, and Km13). Growth curve, protein level, and protein maximum activity occurred on day-3. The largest diameter of clear zone of six isolates was Km25, Sr75, Jm, U6, G8, and Km13 (3.32, 3.31, 2.41, 2.36, 2.19, and 2.04 mm, respectively). Endoglucanase and exoglucanase maximum activity were 0.011-0.402 IU/mL and 0.0028-0.155 IU/mL, respectively. Conclusion: Six isolates showed high activities of cellulase with diameter of clear zone ≥ 2 cm (Km25, Sr75, Jm, U6, G8, and Km13). Growth maximum curve was on day-3. Highest endo- and exoglucanase activities were on day-3 (0.402 IU/mL and 0.155 IU/mL, respectively) in Jm isolate. Keywords: Identification, degradation, clear zones, cellulase.

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Development of Bacterial Consortia for Solid Waste Degradation and its Use as Organic Manure

International Journal of Agriculture Environment and Biotechnology ◽

10.30954/0974-1712.04.2020.5 ◽

2020 ◽

Vol 13 (4) ◽

Author(s):

Suparna Deepak

Keyword(s):

Solid Waste ◽

Organic Waste ◽

Microbial Consortia ◽

Solid Waste Disposal ◽

Biochemical Tests ◽

Promoting Effect ◽

Bacterial Consortia ◽

Organic Solid ◽

Plant Growth Promoting ◽

Waste Degradation

Solid waste disposal has become a big concern all over the world. Almost 50% of the municipal waste collected daily, especially in the metropolitan cities where the population is very high, consists of organic solid waste. This organic waste left in the open dumping grounds results in stench and contamination of groundwater. The present study aims to find out the utilization of effective microbial consortia for decomposing the organic waste. Different garbage samples was collected from Panvel area for isolating the effective organic waste degrading organisms. These strains were characterized by microscopic observations and biochemical tests. Their cellulolytic, proteolytic, amylolytic and lipoolytic activities were evaluated. Depending on these enzyme activities, different consortia were prepared and their efficacy in reduction, maturity and deodorization of organic waste was compared. The plant growth promoting effect of this degraded waste on Triticum aestivum (wheat) and Zea mays (maize) was evaluated. 5 Different Microbial Consortium was developed. Microbial consortia increased the efficacy of composting as compared to control. Consortia no. 1 and 5 showed better degrading ability. Consortia 4 and 5 facilitated the best growth for Wheat and Maize respectively. Consortia 2 showed the best result in terms of germination and growth for both wheat and maize

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Karakteristik dan Potensi Enzimatis Bakteri Asal Tanah Sampah Dapur dan Kotoran Ternak sebagai Kandidat Agen Biodegradasi Sampah Organik

Al-Hayat: Journal of Biology and Applied Biology ◽

10.21580/ah.v4i1.7013 ◽

2021 ◽

Vol 4 (1) ◽

pp. 11-18

Author(s):

Taruna Dwi Satwika ◽

Dwiana Muflihah Yulianti ◽

Arif Rahman Hikam

Keyword(s):

Organic Waste ◽

Soil Samples ◽

Cow Dung ◽

Cellulolytic Activity ◽

Bacterial Isolates ◽

Kitchen Waste ◽

Proteolytic Activities ◽

Waste Degradation ◽

Screening Result ◽

Selection Of

Composting is an alternative for recycling organic waste. Microorganisms that can degrade the components of organic waste are an essential part of composting. Isolation and selection of bacteria with the ability to degrade the elements of organic waste are the first steps to obtain an organic waste degrading agent. This study aimed to determine the characteristics and the enzymatic potential (cellulolytic, amylolytic, and proteolytic) of bacteria isolated from soil, cow dung and kitchen waste as candidates for organic waste degradation agents. The research begins with sampling and isolation of bacteria. The isolates obtained were tested qualitatively for their amylolytic, cellulolytic and proteolytic activities. Furthermore, bacterial isolates were characterized morphologically and biochemically. A total of 30 bacterial isolates were isolated from soil samples, cow dung and kitchen waste. The screening result showed that 6 isolates had amylolytic activity, 7 isolates had cellulolytic activity and 3 isolates had proteolytic activity. These bacterial isolates showed various morphological and biochemical characteristics. In general, soil samples, kitchen waste and cow dung contain potential bacteria as organic waste degrading agents

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Bioaugmentation by Pediococcus acidilactici AAF1-5 Improves the Bacterial Activity and Diversity of Cereal Vinegar Under Solid-State Fermentation

Frontiers in Microbiology ◽

10.3389/fmicb.2020.603721 ◽

2021 ◽

Vol 11 ◽

Author(s):

Qiang Zhang ◽

Cuimei Zhao ◽

Xiaobin Wang ◽

Xiaowei Li ◽

Yu Zheng ◽

...

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Fermentation Process ◽

Raw Materials ◽

Lower Layer ◽

Amino Nitrogen ◽

Community Diversity ◽

Volatile Acid ◽

Pediococcus Acidilactici ◽

Highly Correlated

Bioaugmentation technology may be an effective strategy to improve the solid-state fermentation rate and utilization of raw materials for traditional vinegar production. The relationship between bacteria and fermentation process was analyzed to rationally design and perform bioaugmented solid-state fermentation of the Tianjin Duliu mature vinegar (TDMV). Fermentation process was highly correlated with Acetobacter, Lactobacillus, and Pediococcus contents, which were the core functional microorganisms in TDMV fermentation. Pediococcus acidilactici AAF1-5 was selected from 20 strains to fortify the fermentation due to its acidity and thermal tolerance. Bioaugmentation was performed in the upper layer of TDMV fermentation. P. acidilactici AAF1-5 colonized and then spread into the lower layer to improve the fermentation. Result showed that the fermentation period was 5 days less than that of the control. Meanwhile, the non-volatile acid, lactic acid, amino nitrogen, and reducing sugar contents in the bioaugmented TDMV increased by 53%, 14%, 32%, and 36%, respectively, compared with those in the control. Bioaugmentation with P. acidilactici AAF1-5 not only improved the utilization of starch from 79% to 83% but also increased the bacterial community diversity.

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