scholarly journals Short communication: Endophytic actinobacteria isolated from ginger (Zingiber officinale) and its potential as a pancreatic lipase inhibitor and its toxicity

2019 ◽  
Vol 20 (5) ◽  
Author(s):  
SRI RAHAYU ◽  
LENNI FITRI ◽  
YULIA SARI ISMAIL
Keyword(s):  
Pancreatic Lipase ◽  
Short Communication ◽  
Ethanol Extract ◽  
Morphological Diversity ◽  
Zingiber Officinale ◽  
Lipase Inhibitor ◽  
New Information ◽  
Ginger Rhizome ◽  
Pancreatic Lipase Inhibitors ◽  
Endophytic Actinobacteria

Abstract. Rahayu S, Fitri L, Ismail YS. 2019. Short communication: Endophytic actinobacteria isolated from ginger (Zingiber officinale) and its potential as a pancreatic lipase inhibitor and its toxicity. Biodiversitas 20: 1312-1317.  Endophytic actinobacteria from ginger (Zingiber officinale Rosc.) is a bacterium that is capable of producing secondary metabolites that are the same as their hosts. This study aims to look at the potential of endophytic actinobacteria from ginger as a pancreatic lipase inhibitor and its toxicity. Endophytic actinobacteria were isolated, purified, then tested for pancreatic lipase inhibitors and their toxicity using the BSLT method (Brine Shrimp Lethality Test) and phytochemical tested on ethanol extract of selected isolates. Seven endophytic actinobacterial isolates were isolated from the ginger rhizome. The isolates had different morphological diversity based on colony and microscopic observations and 5 isolates had pancreatic lipase inhibitor activity. The highest inhibitors were found in AJ4 isolates (89.9%), compared with pancreatic lipase inhibitors crude extracts of ginger (68.9%) and orlistat (88.1%) as positive controls. The LC50 value of AJ4 isolates was 653,381 ppm and the value of LT50 was 17,569 hours. AJ4 isolates contain terpenoids, phenols, tannins, flavonoids, alkaloids, and saponins. This research data is considered as new information about the potential of endophytic actinobacteria from ginger as pancreatic lipase inhibitors and their toxicity.

scholarly journals Characterization of Ethanolic Extract of Streptomyces sp. As a Pancreatic Lipase Inhibitors Produced by Endophytic Streptomyces sp. AEBg12

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Lenni Fitri ◽  
Anja Meryandini ◽  
Dyah Iswantini ◽  
Yulin Lestari
Keyword(s):  
Tissue Culture ◽  
Pancreatic Lipase ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Phytochemical Analysis ◽  
Streptomyces Sp ◽  
Lipase Inhibitor ◽  
Inhibitory Compound ◽  
Endophytic Streptomyces ◽  
Inhibitor Compounds

<p>Endophytic <em>Streptomyces </em>sp. AEBg12 isolated from <em>Zingiber</em><em> </em><em>cassumunar</em><em> </em>(Bangle) is known to produce pancreatic lipase inhibitory compound. However, the characteristics of this active compound has not been reported yet. This study aimed to determine the characteristics of pancreatics inhibitory compound produced by <em>Streptomyces </em>sp. AEBg12 and to assess the role of endophytic actinobacteria in producing pancreatic lipase inhibitor using endophytic-free bangle tissue culture, wild bangle and compared with the activity of <em>Streptomyces </em>sp. AEBg12 endophytes. Supernatant of <em>Streptomyces </em>sp. AEBg12 was extracted using ethanol, ethyl acetate, and n-hexane solvents. Toxicity test was performed using larvae of shrimp <em>Artemia</em><em> </em><em>salina</em>. The results showed that the best solvent to obtain pancreatic lipase inhibitor compounds was ethanol. Phytochemical analysis showed that ethanolic extract of endophytic <em>Streptomyces </em>sp. AEBg12 contained flavonoids. IC<sub>50</sub> value of ethanol extract was 180.83 µg/ml. The result of TLC showed that ethanolic extract of <em>Streptomyces </em>AEBg12 had a blue luminescence band indicated that there were either flavone, flavanones, flavonols or isoflavones. Inhibitory activity of <em>Streptomyces </em>sp. AEBg12 was higher than wild bangle and bangle tissue culture. The benefit of this study was to obtain new compound which might be used as an antiobesity drug through approach to the pancreatic lipase inhibitor.</p>

scholarly journals Pancreatic Lipase Inhibitory Activity of Endophytic Actinobacteria from Rhododendron spp.

2021 ◽  
Vol 13 (2) ◽  
pp. 178-184
Author(s):  
Witri Winanda ◽  
Irmanida Batubara ◽  
Yulin Lestari
Keyword(s):  
Pancreatic Lipase ◽  
Inhibitory Activity ◽  
Morphological Character ◽  
Phytochemical Analysis ◽  
Rrna Gene ◽  
Lipase Inhibitor ◽  
Streptomyces Spp ◽  
Ic50 Value ◽  
Therapeutic Compounds ◽  
Endophytic Actinobacteria

Antiobesity medication is available as therapeutic compounds that can reduce fat digestion by the inhibition of pancreatic lipase. Actinobacteria have the potency as source of bioactive compounds with various biological function including as pancreatic lipase inhibitor.  However, the potency of endophytic actinobacteria from Rhododendron spp. as source of pancreatic lipase inhibitor producer has not been reported yet. The aim of this study was to examine the potential of pancreatic lipase inhibitory activity of 23 endophytic actinobacteria from Rhododendron spp.; to characterize their colony based on morphology and molecular analysis. Screening test of pancreatic lipase inhibitor was conducted using the supernatant of endophytic actinobacteria, lipase pancreatic porcine (L3126) and p-nitrophenyl butyrate. The supernatant of selected isolates was extracted using ethyl acetate. The result showed that various inhibitory activities ranging between 0.00 until 91.69%. There were 11 out of 23 isolates that have potential as pancreatic inhibitor. Amongst them, the extract of four selected isolates, i.e. RZP 1.3, RSSB 3.2, RSS 2.1, and RJB F3.2 demonstrated inhibitory percentage of more than 80%. The RJB F3.2 extract showed to have IC50 value by 431.48 µg mL-1 compared to control, i.e. Xenical (89.07 µg mL-1). Phytochemical analysis exhibited that the extract of the selected isolates contained alkaloid which may function as pancreatic lipase inhibitor. Based on the morphological character, the selected isolates have various morphological colonies and 16S rRNA gene sequence revealed the sequence homology to Streptomyces spp. The data clearly indicate that endophytic actinobacteria from Rhododendron spp. have potency as pancreatic lipase inhibitor producer and further studies could be explored for the development of antiobesity agent.

scholarly journals The Endophytic Actinobacterial Toxicity Test of Ginger (Zingiber officinale Roscoe) Used The BSLT (Brine Shrimp Lethality Test) Method

Elkawnie ◽  
2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Sri Rahayu ◽  
Lenni Fitri ◽  
Yulia Sari Ismail
Keyword(s):  
Cancer Patients ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Ethanol Extract ◽  
Zingiber Officinale ◽  
Test Method ◽  
Brine Shrimp Lethality ◽  
Best Value ◽  
Zingiber Officinale Roscoe ◽  
Endophytic Actinobacteria

Abstract: Cancer is the biggest cause of death in Indonesia. Cancer treatment efforts have been made but it could harm cancer patients. It is necessary to find another alternative from nature as an anticancer. This study aims to see the ability of endophytic actinobacterial isolates from ginger (Zingiber officinale) as an anticancer tested using the BSLT method (Brine Shrimp Lethality Test) by calculating the values of LC50 and LT50. The best value isolates from the BSLT method, then tested using phytochemical ethanol extract. The highest toxicity value was found in AJ2 isolates. LC50 and LT50 values of AJ2 isolates were 309.358 µg/ml and 11.879 hours. Phytochemical results of ethanol extract of AJ2 isolates were detected containing steroids, terpenoids, phenols, tannins, flavonoids, alkaloids, and saponins. AJ2 isolates which derived from ginger endophytic actinobacteria have potential as an anticancer because they have high toxic values that rised rapidly.Abstrak: Kanker menjadi penyebab kematian terbesar di Indonesia. Upaya pengobatan kanker sudah dilakukan namun memberikan dampak negatif bagi penderita kanker. Perlu dilakukan penemuan alternatif lain berasal dari alam sebagai antikanker. Penelitian ini bertujuan untuk melihat kemampuan isolat aktinobakteri endofit asal jahe (Zingiber officinale) sebagai antikanker. 7 isolat aktinobakteri endofit jahe diuji menggunakan metode BSLT (Brine Shrimp Lethality Test) dengan menghitung nilai LC50 dan LT50. Isolat nilai terbaik dari metode BSLT, selanjutnya diuji  menggunakan fitokimia ekstrak etanol. Nilai toksisitas tertinggi terdapat pada isolat AJ2. Nilai LC50 dan LT50 isolat AJ2 adalah 309,358 µg/ml dan 11,879 jam. Hasil fitokimia ekstrak etanol isolat AJ2 terdeteksi mengandung steroid, terpenoid, fenol, tanin, flavonoid, alkaloid dan saponin. Isolat AJ2 yang berasal dari actinobacteria endofit jahe berpotensi sebagai antikanker karena memiliki nilai toksik yang tinggi dalam waktu singkat.

scholarly journals Phytochemistry and GC-MS Screening and Biocidal Potentiality of Ginger (Zingiber officinale) Rhizome against Mosquito’s Larvae

2021 ◽  
Vol 3 (2) ◽  
pp. 090-096
Author(s):  
Dalia Mustafa M Elbashir ◽  
Mutaman AA Kehail ◽  
Abdalla I Abdalla Mohamed ◽  
Abdelmonem Eltiyab H Ali
Keyword(s):  
Aqueous Extract ◽  
Chemical Constituents ◽  
Ethanol Extract ◽  
Zingiber Officinale ◽  
Chemical Components ◽  
Biocidal Activity ◽  
Plant Parts ◽  
Ginger Rhizome ◽  
Ethanol Extracts ◽  
Phytochemical Components

Mosquitoes can act as vectors for many viruses and parasites through carrying these organisms from person to person. Although great number of natural products are lethal to mosquitoes, but few researches are conducted to understand the magnitude of the behavioral response to these plant parts. The objectives of this study were to screen the phytochemical and other chemical components from ginger rhizome in addition to test their aqueous and ethanol extracts on mosquito’s larvae. The standard methods, materials and devices were used to screen the phytochemical components and the chemical constituents (GC-MS). The aqueous and the ethanol extracts from clove pods were prepared and used against Anopheles, Culex and Aedes larvae. The results of the aqueous extract showed that, the LC50 was 153.77 mg/L for Anopheles larvae, 179.05 mg/L for Culex larvae, and 208.37 mg/L for Aedes larvae, also the ethanol extract of ginger rhizome was more potent than the aqueous extract. The biocidal activity can be attributed to the presence of saponins, flavonoids, glycosides, alkaloids and steroids. The GC-MS for the hexane extract showed the presence of alkaloid: gingerol (18%) and eugenyl acetate- (19%) that gives the sweet aromatic, spicy taste, but the ethanol extract detected gingerol (43%) and zingiberene (14%). The obtained data will be useful to understand the mechanisms and reasons of biocidal activity of ginger rhizome against the tested mosquito’s larvae.

scholarly journals Solid Soap Formulation with Ethanol Extract of Ginger Rhizome (Zingiber officinale Rosc.) Combination of Virgin Coconut Oil (Vco) and Palm Oil

2021 ◽  
Vol 8 (2) ◽  
pp. 62
Author(s):  
Risma Andriani ◽  
Yani Ambari ◽  
Iif Hanifa Nurrosyidah
Keyword(s):  
Palm Oil ◽  
Foam Stability ◽  
Coconut Oil ◽  
Ethanol Extract ◽  
Zingiber Officinale ◽  
Cleaning Product ◽  
E Coli ◽  
Wide Range ◽  
Ginger Rhizome ◽  
Chemically Reacting

Soap is a type of cleaning product that is used to clean dust, oil, dirt, germs, and bacteria that stick to the skin. Ginger is a plant that contains a wide range of antibacterial compounds including E. Coli, Staphylococcus aureus, B. Cereus, L monocytogenes and Candida albicans bacteria. Soap is made by chemically reacting sodium or potassium compounds with fatty acids derived from vegetable oil or animal oil. This study aims to determine the physical characteristics of solid soap with ginger rhizome extract with a combination of coconut oil and palm oil and analyze the panelist’s response to the soap produced. In the third week of observation, it is known that the water content ranges from 0,07%-0,1% with foam stability 71,11%-88,23% and pH 8,96-9,08. The most preferred solid soap based on the response of the panelist is soap from a mixture of coconut oil and palm oil with a ratio of 15:5.

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