scholarly journals Abundance and characterization of microorganisms isolated from oil palm empty fruit bunches waste under aerobic, anaerobic, and facultative anaerobic conditions

2020 ◽  
Vol 21 (9) ◽  
Author(s):  
DERMIYATI DERMIYATI ◽  
RADIX SUHARJO ◽  
MARELI TELAUMBANUA ◽  
RULLY YOSITA ◽  
ANGGI WINANDA SARI ◽  
...  
Keyword(s):  
Oil Palm ◽  
Anaerobic Condition ◽  
Bacterial Abundance ◽  
Plate Count ◽  
Cellulose Content ◽  
Anaerobic Conditions ◽  
Empty Fruit Bunches ◽  
Facultative Anaerobic ◽  
Different Characteristics

Abstract. Dermiyati, Suharjo R, Telaumbanua M, Yosita R, Sari AW, Andayani AP. 2020. Abundance and characterization of microorganisms isolated from oil palm empty fruit bunches waste under aerobic, anaerobic, and facultative anaerobic conditions. Biodiversitas 21: 4213-4220. Increased production of palm oil causes an increase in oil palm empty fruit bunches waste, while the utilization of the waste has not been carried out optimally. Oil palm empty fruit bunches contain high cellulose content which promotes the development of various types of bacteria with different characteristics. This study was aimed to determine the abundance and the characterization of bacteria that were isolated from extract suspension of oil palm empty fruit bunches in the form of a local microorganism solution under aerobic, anaerobic, and facultative anaerobic conditions. For isolation, the oil palm empty fruit extract suspension was inoculated on media Plate Count Agar Peptone and bacterial abundance was determined. Various bacterial characterization test included bacterial morphology test in the form of color and shape of macroscopic isolates, gram test using KOH 3%, fermentative oxidative test, soft rot test on potato tubers, hypersensitivity test on tobacco plant leaves, and hypo virulent test which was indicated by the presence of symptoms of disease in cucumber sprouts and indicated in the disease severity index (DSI) was carried out. The results of local microorganism solution isolation from oil palm empty fruit bunches obtained as many as 220 bacterial isolates consisting of 84 isolates in aerobic condition, 68 isolates in anaerobic condition, and 68 isolates in facultative anaerobic condition. The highest bacterial abundance in aerobic conditions was equal to 285.60 x 1010 CFU mL-1. Each bacterial isolate had different characteristics. Most of the characteristics of bacteria were turbid white (34.55%), round (69.55%), gram-positive (76.36%), fermentative (89.55%), softrot negative (71.82%), hypersensitive negative (97.27%), and virulent (74.55%).

scholarly journals Pengaruh Penambahan Nanoselulosa dari Tandan Kosong Kelapa Sawit (Elaeis guinensis Jacq) terhadap Karakteristik Polivinil Alkohol (PVA)

2020 ◽  
Vol 5 (2) ◽  
pp. 203-209
Author(s):  
Fathia Arami B Tou ◽  
Eti Indarti ◽  
Ismail Sulaiman
Keyword(s):  
Oil Palm ◽  
Water Vapor Permeability ◽  
Vapor Permeability ◽  
Cellulose Content ◽  
Empty Fruit Bunches ◽  
Descriptive Research ◽  
Two Factors ◽  
Ft Ir ◽  
High Cellulose

Abstrak: Tandan kosong kelapa sawit merupakan limbah padat terbesar yang dihasilkan oleh perkebunan kelapa sawit. Kandungan utama tandan kosong kelapa sawit adalah selulosa. Tingginya kandungan selulosa pada tandan kosong kelapa sawit dapat dimanfaatkan sebagai bahan dasar pembuatan nanoselulosa. Nanoselulosa merupakan selulosa yang dihasilkan dalam skala nano dan memiliki sifat karakteristik yang jauh lebih baik dibandingkan dengan selulosa. Pada penelitian ini dilakukan isolasi selulosa yang berasal dari tandan kosong kelapa sawit untuk menghasilkan nanoselulosa yang dapat dimanfaatkan sebagai pencampur (filler) pada polimer polivinil alkohol (PVA), sehingga diharapkan dapat memperbaiki karakteristik pada PVA. Tujuan dari penelitian ini yaitu untuk mengkaji isolasi selulosa menjadi nanoselulosa dari tandan kosong kelapa sawit serta mempelajari karakteristik pada PVA dengan adanya penambahan nanoselulosa. Penelitian ini dilakukan menggunakan rancangan penelitian deskriptif yang terdiri dari jumlah penambahan nanoselulosa (N) dan jumlah PVA (P). Karakterisasi yang dilakukan adalah uji ketebalan, uji kuat tarik, uji FT-IR, uji WVP, dan uji UV-Vis Spectrophotometer. Hasil dari penelitian menunjukkan bahwa penambahan nanoselulosa berpengaruh terhadap karakteristik film PVA. The Effects of Adding Nanocellulose From Oil Palm Empty Fruit Bunch (Elaeis guinensis Jacq) For Characterization of Polyvinil Alcohol (PVA) Abstract: Oil palm empty fruit bunches (OPEFB) are the largest solid waste produced by oil palm plantations. The main content of oil palm empty fruit bunches is cellulose. High cellulose content in oil palm empty fruit bunches can be used for making nanocellulose. Nanocellulose is cellulose that produced in nanoscale and it has better characteristic properties compared to cellulose. In this study, cellulose from oil palm empty fruit bunches was isolated to produce nanocellulose that can be used as filler for characterization of polyvinil alcohol (PVA). The purpose of this study is to examined the isolation of cellulose into nanocellulose from oil palm empty fruit bunches and to investiage the characteristics of PVA with the addition of nanocellulose. This study was conducted using a descriptive research design consisting of 2 (two) factors. The first factor was the total addition of nanocellulose (N) and the second factor was the amount of polyvinyl alcohol (P). The characterization that carried out were a thickness test, tensile strength, Fourier Transform Infra-Red (FT-IR), Water Vapor Permeability (WVP), and UV-Vis Spectrophotometer. The results of the study showed that the addition of nanocellulose can effect the characteristics of PVA films. 

scholarly journals Activation of oil palm empty bunches with hydrochloride acid & phosphoric acid and characterization based SNI No. 06-3730-1995

2021 ◽  
Vol 11 (1) ◽  
pp. 16
Author(s):  
Irna Diah Mumpuni ◽  
Mamay Maslahat ◽  
Devy Susanty
Keyword(s):  
Activated Carbon ◽  
Moisture Content ◽  
Phosphoric Acid ◽  
Oil Palm ◽  
Ash Content ◽  
National Standard ◽  
Empty Fruit Bunches ◽  
Different Characteristics

Activated carbon is a solid product resulting from carbonization and activation of lignocellulosic biomass by several methods and activators. Each activator can produce different characteristics of activated carbon. The activated carbon of Oil Palm Empty Fruit Bunches (OPEFB) is processed from Empty Fruit Bunches (EFB) waste, which is quite abundant in Indonesia. The purpose of this study was to determine the characteristics of OPEFB carbon that had been chemically activated using hydrochloric acid (HCl) and phosphoric acid (H3PO4) based on the Indonesian National Standard (SNI) No. 06 - 3730 - 1995. The research method consisted of the process of carbonization of OPEFB using a pyrolysis device at 400oC for 6 hours; activation of carbon with activator HCl and H3PO4 at various activator concentrations; characterization of activated carbon on the parameters of moisture content, ash content, absorption of iodine, and absorption of methylene blue. The results showed that the 0.5000 M HCl was the best activator that produced the quality of OPEFB activated carbon according to SNI 06 - 3730 - 1995 on parameters of moisture content, ash content and absorption of iodine.Keywords: Activated Carbon, Iodine Absorption, Oil Palm Empty Fruit BunchesABSTRAKAktivasi karbon tandan kosong kelapa sawit dengan asam klorida dan asam fosfat serta uji karakterisasi berdasarkan SNI No. 06–3730–1995Karbon aktif merupakan produk padatan yang dihasilkan dari karbonisasi dan aktivasi biomassa lignoselulosa dengan beberapa metode dan aktivator. Masing-masing aktivator dapat menghasilkan karakteristik karbon aktif yang berbeda. Karbon aktif Tandan Kosong Kelapa Sawit (TKKS) diolah dari limbah TKKS yang keberadaannya cukup melimpah di Indonesia. Tujuan penelitian ini adalah mengetahui karakteristik karbon TKKS yang telah diaktivasi secara kimia menggunakan aktivator asam klorida (HCl) dan asam fosfat (H3PO4) berdasarkan Standar Nasional Indonesia  (SNI) No.  06 – 3730 – 1995. Metode penelitian terdiri atas proses karbonisasi TKKS menggunakan alat pirolisis pada suhu 400 ℃ selama 6 jam; aktivasi karbon dengan aktivator HCl dan H3PO4 pada berbagai konsentrasi aktivator; serta karakterisasi karbon aktif pada parameter kadar air dan abu, daya jerap terhadap iod serta daya jerap terhadap biru metilen. Hasil penelitian menunjukkan bahwa HCl 0,5 M merupakan aktivator terbaik dan karbon aktif yang dihasilkan memiliki karakteristik yang sesuai dengan SNI 06 – 3730 – 1995 pada parameter kadar air dan abu serta daya jerap terhadap iod.Kata kunci: Daya Jerap Iod, Karbon Aktif, Tandan Kosong Kelapa Sawit

scholarly journals Eco-Friendly Extraction and Characterization of Cellulose from Oil Palm Empty Fruit Bunches

BioResources ◽  
2013 ◽  
Vol 8 (2) ◽  
Author(s):  
Muhammad Shahid Nazir ◽  
Bambang Air Wahjoedi ◽  
Abdul Wahid Yussof ◽  
Mohd Azmuddin Abdullah
Keyword(s):  
Oil Palm ◽  
Empty Fruit Bunches

GASIFICATION OF OIL PALM EMPTY FRUIT BUNCHES (OPEFB) BRIQUETTES FOR BIO-SYNGAS PRODUCTION

2016 ◽  
Vol 78 (8-3) ◽  
Author(s):  
Bemgba Bevan Nyakuma ◽  
Arshad Ahmad ◽  
Anwar Johari ◽  
Tuan A. T. Abdullah ◽  
Olagoke Oladokun ◽  
...  
Keyword(s):  
Fluidized Bed ◽  
Oil Palm ◽  
Empty Fruit Bunches ◽  
Syngas Production ◽  
Bed Temperature ◽  
Fluidized Bed Gasifier ◽  
Bed Agglomeration ◽  
Solid Biomass Fuel ◽  
Solid Biomass

Gasification of Oil Palm Empty Fruit Bunches (OPEFB) briquettes was investigated in an air blown 4.5 kW allothermal fluidized bed gasifier to examine the effects of bed temperature (600 – 800 °C) and equivalence ratio (λ = 0.25) on bio-syngas yield and composition. In addition, physicochemical and thermochemical characterization of the fuel properties of the OPEFB briquettes were also examined. The results demonstrate that pelletization improved the solid biomass fuel (SBF) properties of OPEFB including moisture content and higher heating value (HHV). The gasification of OPEFB briquettes produced bio-syngas comprising H2, CO, CO2, CH4 as well as solid biochar with a HHV higher than the original OPEFB briquettes. The highest yield of H2 was obtained at 600 °C while HHV of the bio-syngas was within the range 4 - 8 MJ/Nm3 for air gasification in fluidized bed gasifiers. In addition, agglomeration of bed materials did not occur during OPEFB briquettes gasification despite its high bed agglomeration potential (BAP). In conclusion, the gasification of OPEFB briquettes into bio-syngas and biochar is a practical route for bioenergy production in Malaysia

scholarly journals Produksi dan karakterisasi lakase Omphalina sp. Production and characterization of Omphalina sp. laccase

2016 ◽  
Vol 75 (2) ◽  
Author(s):  
. SISWANTO ◽  
. SUHARYANTO ◽  
Rossy FITRIA
Keyword(s):  
Oil Palm ◽  
Laccase Activity ◽  
Research Result ◽  
White Rot Fungi ◽  
White Rot ◽  
Partial Purification ◽  
Empty Fruit Bunches ◽  
Optimum Ph ◽  
Laccase Enzyme

SummaryOmphalina sp. a white-rot fungi (WRF)originated from oil palm plantation has abilityto degrade empty fruit bunches of oil palm(EFBOP) so that it is expected to producelaccase with high activity. The ability ofOmphalina sp. to produce laccase enzyme onliquid fermentation will be studied. The enzymewill also be partially purified andcharacterized. The research result showed thatthe highest enzyme activity (1.162 U/mL) wasobtained using glucose malt yeast (GMY)medium at room temperature for four days.The addition of 2,5-xylidine as an inducerproduced laccase earlier i.e two days, but theactivity of laccase was less active afterprolonged incubation compared to that ofcontrol. The laccase produced on mediumcontaining 2% EFBOP reached optimumactivity as much as 0.38 U/mL after 10 th daysof incubation. Partial purification of laccaseon Sephacryl S-200 HR column resulted58.23% of yield recovery with twice purity thanbefore. The optimum pH of laccase was 4.5.Laccase activity was stable even after heatedon 50 o C for 30 minutes, but then decreasedwhen heated until 60 o C. The laccase has K Mand V max as much as 0.15 mM and 0.56 U/mLrespectively.RingkasanOmphalina sp., adalah fungi pelapuk putih(FPP) hasil isolasi dari kebun kelapa sawityang diketahui mampu mendegradasi tandankosong kelapa sawit (TKKS) dengan cepatsehingga diharapkan mampu menghasilkanlakase dengan aktivitas tinggi. KemampuanOmphalina sp. menghasilkan enzim lakasepada fermentasi cair akan dipelajari. Selain itu,lakase yang dihasilkan akan dimurnikan secaraparsial serta dilakukan karakterisasi pH, suhu,dan konsentrasi substrat optimum. Hasilpenelitian menunjukkan bahwa Omphalina sp.menghasilkan lakase dengan aktivitas tertinggi(1,162 U/mL) pada medium glucose malt yeast(GMY) yang diinkubasikan pada suhu ruangselama empat hari. Penambahan 2,5-xilidinsebagai induser mempercepat produksi lakaselebih awal yaitu dalam waktu dua hari, namunaktivitasnya masih lebih rendah dibandingkandengan kontrol pada inkubasi lebih lanjut.Lakase dari Omphalina sp. juga dapatdiproduksi pada medium yang mengandung2% TKKS dan aktivitasnya mencapai0,38 U/mL yang diinkubasi dalam suhu ruangselama 10 hari. Pemurnian parsial pada kolomSephacryl S-200 HR menghasilkan rendemensebesar 58,23% dengan kemurnian dua kalinya.Aktivitas lakase optimum pada pH 4,5 dantetap stabil setelah pemanasan selama 30 menitpada suhu ruang hingga 50 o C dan menuruntajam pada suhu 60 o C. Lakase Omphalina sp.menghasilkan nilai K M dan V maks masing-masing sebesar 0,15 mM dan 0,56 U/mL.

scholarly journals Kemampuan jamur pelapuk kayu isolat JPA dan Trichoderma Sp. S2-2 dalam mendegradasi tandan kosong kelapa sawit untuk menghasilkan selulosa The capability of wood rot fungus JPA isolate and Trichoderma sp. S2-2 in degradation of oil palm empty fruit bunches to produce cellulose

2016 ◽  
Vol 82 (2) ◽  
Author(s):  
. ALHIDAYATULLAH ◽  
Lisdar I SUDIRMAN1 ◽  
Okky Setyawati DHARMAPUTRA
Keyword(s):  
Palm Oil ◽  
Oil Palm ◽  
Trichoderma Harzianum ◽  
Raw Materials ◽  
Value Added ◽  
Cellulose Content ◽  
Empty Fruit Bunches ◽  
Oil Processing ◽  
Trichoderma Sp ◽  
Value Added Products

Abstract  Oil palm empty fruit bunches (OPEFB) are the ligno-cellulosic wastes from palm oil processing. They can be used to produce raw materials for value-added products. The purpose of this study was to determine the degradation capacity of JPA wood rot fungi and Trichoderma sp. S2-2 on OPEFB. The 500 g of substrates consisted of 81% of OPEFB, 15% bran, 1.5% lime and 1.5% gypsum were used for growing. The substrates were inoculated with five treatments i.e without isolate (K); with JPA isolate (JPA); with Trichoderma sp. S2-2 (T); with the two isolates (JPA + T); and with JPA isolate and after four weeks of incubation inoculated with Trichoderma sp. S2-2 [(JPA)+T]. All treatments were incubated for eight weeks. The results showed that JPA+T was the best treatment which the two isolates must be inoculated simultaneously for degradation of OPEFB. Lignin and cellulose content on JPA+T treatment respectively were 20.83% and 33.77%. C/N ratio of OPEFB degraded with JPA+T was lower than the C/N ratio of TKKS degraded with Trichoderma harzianum and TKKS degraded with EM4 in previous study. AbstrakTandan kosong kelapa sawit (TKKS) merupakan limbah lignoselulosa dari pengolahan minyak kelapa sawit. TKKS dapat dimanfaatkan untuk memperoleh bahan baku untuk produk bernilai tambah. Tujuan penelitian ini adalah untuk mengetahui kemampuan degradasi jamur pelapuk kayu isolat JPA dan Trichoderma sp. S2-2 pada TKKS. Sebanyak 500 g substrat terdiri dari 81% TKKS, 15% dedak, 1,5% kapur, dan 1,5% gypsum digunakan untuk per-tumbuhan. Substrat diinokulasi dengan lima perlakuan yaitu tanpa isolat (K); dengan isolat JPA (JPA); dengan Trichoderma sp. S2-2 (T); dengan isolat JPA dan setelah empat minggu inkubasi, diinokulasi dengan Trichoderma sp. S2-2 [(JPA+T)]. Semua perlakuan diinkubasi selama delapan minggu. Hasil percobaan menunjukkan bahwa perlakuan JPA+T adalah perlakuan terbaik yaitu kedua isolat tesebut harus diinokulasi secara bersamaan untuk mendegradasi TKKS. Kandungan lignin dan selulosa TKKS dengan  perlakuan  JPA+T   masing-masing  adalah  20,83% dan 33,77%. Rasio C/N TKKS hasil degradasi dengan JPA+T lebih  rendah  daripada  rasio C/N pada TKKS yang didegradasi dengan Trichoderma harzianum dan TKKS yang didegradasi dengan EM4 pada penelitian sebelumnya.

scholarly journals CONDITION OPTIMIZATION PRODUCTION XYLANASE THERMOSTABLE BY Bacillus licheniformis TS10 USING SUBSTRATE OIL PALM EMPTY FRUIT BUNCHES

2017 ◽  
Vol 3 (1) ◽  
Author(s):  
Ayu Safitri ◽  
Muharni ◽  
Heni Yohandini Kusumawati
Keyword(s):  
Bacillus Licheniformis ◽  
Oil Palm ◽  
Substrate Concentration ◽  
Plate Count ◽  
Bacterial Cells ◽  
Thermostable Xylanase ◽  
Xylanase Production ◽  
Standard Curve ◽  
Empty Fruit Bunches ◽  
Condition Optimization

Optimization conditions for thermostable xylanase production of Bacillus licheniformis TS10 by using substrate oil palm empty fruit bunches  has been conducted from November 2015 to January 2016 in the Laboratory of Genetics and Biotechnology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sriwijaya, Indralaya. The aims of this study was to determine the potential of oil palm empty fruit bunches ( EFB ) as a substrate for the production of thermostable xylanase by Bacillus licheniformis TS10 and the optimum conditions of temperature, pH and substrate concentration in the fermentation process. The research method is to make the growth curve and the curve of Bacillus licheniformis TS10 enzyme production on EFB substrate. The number of bacterial cells was determined by using a UV-VIS spectrophotometer and total plate count (TPC) through a standard curve. Optimization performed at various pH (5, 6, 7, 8, 9), temperature (50 °C, 60 °C, 70 °C, 80 °C) and substrate (1%, 2%, 3%, 4%) , Test activity at each pH, temperature and substrate using methods DNS by measuring enzyme activity based on the reducing sugar released by the substrate by using dinitrosalicylic acid (DNS). Based on the results of this research showed that oil palm empty fruit bunches (EFB ) has potential as a substrate for the production of thermostable xylanase by Bacillus licheniformis TS10, thermostable xylanase production of Bacillus licheniformis TS10 on the substrate EFB has an optimum condition at pH 6, 80 °C and the substrate concentration of 4%.

scholarly journals Preparation of composites from natural rubber and oil palm empty fruit bunch cellulose: Effect of cellulose morphology on properties

BioResources ◽  
2019 ◽  
Vol 14 (2) ◽  
pp. 3168-3181 ◽  
Author(s):  
José Antonio Fiorote ◽  
Alair Pereira Freire ◽  
Dasciana de Sousa Rodrigues ◽  
Maria Alice Martins ◽  
Larissa Andreani ◽  
...  
Keyword(s):  
Natural Rubber ◽  
Oil Palm ◽  
Rubber Tree ◽  
Natural Rubber Latex ◽  
Oil Industry ◽  
Cellulose Content ◽  
Rubber Latex ◽  
Nanostructured Particles ◽  
Empty Fruit Bunches ◽  
Cellulose Composites

Rubber tree and oil palm are industrial crops cultivated in the same climate and environment. These plants are used to prepare nanocomposites of natural rubber and cellulose from empty fruit bunches, an abundant residue in the palm oil industry. For this study, the cellulose particles were extracted from the bunches and subjected to enzymatic hydrolysis or microfibrillation to produce nanostructured particles. The nanoparticles were blended with natural rubber latex in an aqueous medium, and the mixture was dried. The properties of the nanocomposites were compared to those of pure natural rubber and unprocessed cellulose composites. The mechanical properties of the natural rubber can be modified by the cellulose content and morphology. As a consequence, it is possible to modulate the material properties by changing only the filler morphology. The use of microfibrillated cellulose had stronger reinforcement effects. The thermal properties of natural rubber were not affected by the addition of cellulose.

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