scholarly journals CONDITION OPTIMIZATION PRODUCTION XYLANASE THERMOSTABLE BY Bacillus licheniformis TS10 USING SUBSTRATE OIL PALM EMPTY FRUIT BUNCHES

2017 ◽  
Vol 3 (1) ◽  
Author(s):  
Ayu Safitri ◽  
Muharni ◽  
Heni Yohandini Kusumawati
Keyword(s):  
Bacillus Licheniformis ◽  
Oil Palm ◽  
Substrate Concentration ◽  
Plate Count ◽  
Bacterial Cells ◽  
Thermostable Xylanase ◽  
Xylanase Production ◽  
Standard Curve ◽  
Empty Fruit Bunches ◽  
Condition Optimization

Optimization conditions for thermostable xylanase production of Bacillus licheniformis TS10 by using substrate oil palm empty fruit bunches  has been conducted from November 2015 to January 2016 in the Laboratory of Genetics and Biotechnology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sriwijaya, Indralaya. The aims of this study was to determine the potential of oil palm empty fruit bunches ( EFB ) as a substrate for the production of thermostable xylanase by Bacillus licheniformis TS10 and the optimum conditions of temperature, pH and substrate concentration in the fermentation process. The research method is to make the growth curve and the curve of Bacillus licheniformis TS10 enzyme production on EFB substrate. The number of bacterial cells was determined by using a UV-VIS spectrophotometer and total plate count (TPC) through a standard curve. Optimization performed at various pH (5, 6, 7, 8, 9), temperature (50 °C, 60 °C, 70 °C, 80 °C) and substrate (1%, 2%, 3%, 4%) , Test activity at each pH, temperature and substrate using methods DNS by measuring enzyme activity based on the reducing sugar released by the substrate by using dinitrosalicylic acid (DNS). Based on the results of this research showed that oil palm empty fruit bunches (EFB ) has potential as a substrate for the production of thermostable xylanase by Bacillus licheniformis TS10, thermostable xylanase production of Bacillus licheniformis TS10 on the substrate EFB has an optimum condition at pH 6, 80 °C and the substrate concentration of 4%.

scholarly journals Xylitol Production from Oil Palm Empty Fruit Bunches (OPEFB) Via Simultaneous Enzymatic Hydrolysis and Fermentation Process

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Efri Mardawati ◽  
Nadya Maharani ◽  
Dwi Wahyudha Wira ◽  
Budi Mandra Harahap ◽  
Tri Yuliana ◽  
...  
Keyword(s):  
Oil Palm ◽  
Substrate Concentration ◽  
Specific Growth ◽  
Descriptive Analysis ◽  
Debaryomyces Hansenii ◽  
Raw Material ◽  
Xylitol Production ◽  
Empty Fruit Bunches ◽  
Ssf Process ◽  
Specific Growth Rates

Oil palm empty fruit bunches (OPEFB) are the waste of palm oil processing and lignocellulosic biomass that can be used as raw material for xylitol production. In this research, bioconversion of xylitol using the Simultaneous Saccharification and Fermentation (SSF) process in one reactor with a short time and could save the cost. Pretreatment requires to degrade the lignin compound with thermal pretreatment. Hydrolysis of OPEFB performs enzymatically by commercial xylanase enzyme that is Cellic Htec2 and continued with fermentation by yeast Debaryomyces hansenii. The research used experimental and descriptive analysis that covers of variation of OPEFB substrate concentration (7.5%, 10%, 15%, 20%) on specific growth rate (µ), the yield of xylitol and substrate utilization. The results showed that an increase of OPEFB substrate concentration affected by the increase of specific growth rates  (μ) are 0.091/h, 0.094/h, 0.095/h and 0.126/h. It also affected to the decreasing of xylitol yield on 42 hours fermentation are 0.201 g/g; 0.189 g/g; 0.170 g/g; and 0.104 g/g.

scholarly journals Abundance and characterization of microorganisms isolated from oil palm empty fruit bunches waste under aerobic, anaerobic, and facultative anaerobic conditions

2020 ◽  
Vol 21 (9) ◽  
Author(s):  
DERMIYATI DERMIYATI ◽  
RADIX SUHARJO ◽  
MARELI TELAUMBANUA ◽  
RULLY YOSITA ◽  
ANGGI WINANDA SARI ◽  
...  
Keyword(s):  
Oil Palm ◽  
Anaerobic Condition ◽  
Bacterial Abundance ◽  
Plate Count ◽  
Cellulose Content ◽  
Anaerobic Conditions ◽  
Empty Fruit Bunches ◽  
Facultative Anaerobic ◽  
Different Characteristics

Abstract. Dermiyati, Suharjo R, Telaumbanua M, Yosita R, Sari AW, Andayani AP. 2020. Abundance and characterization of microorganisms isolated from oil palm empty fruit bunches waste under aerobic, anaerobic, and facultative anaerobic conditions. Biodiversitas 21: 4213-4220. Increased production of palm oil causes an increase in oil palm empty fruit bunches waste, while the utilization of the waste has not been carried out optimally. Oil palm empty fruit bunches contain high cellulose content which promotes the development of various types of bacteria with different characteristics. This study was aimed to determine the abundance and the characterization of bacteria that were isolated from extract suspension of oil palm empty fruit bunches in the form of a local microorganism solution under aerobic, anaerobic, and facultative anaerobic conditions. For isolation, the oil palm empty fruit extract suspension was inoculated on media Plate Count Agar Peptone and bacterial abundance was determined. Various bacterial characterization test included bacterial morphology test in the form of color and shape of macroscopic isolates, gram test using KOH 3%, fermentative oxidative test, soft rot test on potato tubers, hypersensitivity test on tobacco plant leaves, and hypo virulent test which was indicated by the presence of symptoms of disease in cucumber sprouts and indicated in the disease severity index (DSI) was carried out. The results of local microorganism solution isolation from oil palm empty fruit bunches obtained as many as 220 bacterial isolates consisting of 84 isolates in aerobic condition, 68 isolates in anaerobic condition, and 68 isolates in facultative anaerobic condition. The highest bacterial abundance in aerobic conditions was equal to 285.60 x 1010 CFU mL-1. Each bacterial isolate had different characteristics. Most of the characteristics of bacteria were turbid white (34.55%), round (69.55%), gram-positive (76.36%), fermentative (89.55%), softrot negative (71.82%), hypersensitive negative (97.27%), and virulent (74.55%).

Thermal Decomposition Kinetics of Torrefied Oil Palm Empty Fruit Bunch Briquettes

2016 ◽  
Vol 10 (3) ◽  
pp. 325-328 ◽  
Author(s):  
Bemgba Nyakuma ◽  
◽  
Arshad Ahmad ◽  
Anwar Johari ◽  
Tuan Abdullah ◽  
...  
Keyword(s):  
Thermal Decomposition ◽  
Oil Palm ◽  
Decomposition Kinetics ◽  
Thermal Decomposition Kinetics ◽  
Kinetic Properties ◽  
Tg Analysis ◽  
Empty Fruit Bunches ◽  
Thermal Lag ◽  
Profile Characteristics ◽  
Kinetics Of

The study is aimed at investigating the thermal behavior and decomposition kinetics of torrefied oil palm empty fruit bunches (OPEFB) briquettes using a thermogravimetric (TG) analysis and the Coats-Redfern model. The results revealed that thermal decomposition kinetics of OPEFB and torrefied OPEFB briquettes is significantly influenced by the severity of torrefaction temperature. Furthermore, the temperature profile characteristics; Tonset, Tpeak, and Tend increased consistently due to the thermal lag observed during TG analysis. In addition, the torrefied OPEFB briquettes were observed to possess superior thermal and kinetic properties over the untorrefied OPEFB briquettes. It can be inferred that torrefaction improves the fuel properties of pelletized OPEFB for potential utilization in bioenergy conversion systems.

APPLICATION OF OIL PALM EMPTY FRUIT BUNCH FIBERS IN STRENGTH IMPROVEMENT AS A FIBER REINFORCED CONCRETE

2017 ◽  
Vol 25 (3) ◽  
pp. 161-170
Author(s):  
Henny Lydiasari ◽  
Ari Yusman Manalu ◽  
Rahmi Karolina
Keyword(s):  
Tensile Strength ◽  
Compressive Strength ◽  
Crack Length ◽  
Oil Palm ◽  
Fiber Reinforced Concrete ◽  
Structural Concrete ◽  
Empty Fruit Bunches ◽  
Construction Products ◽  
By Products ◽  
Strength Improvement

The potency of oil palm empty fruit bunches (OPEFB) fibers as one of the by-products of processing oil palm is increasing significantly so that proper management is needed in reducing environmental impact. One of the utilization of OPEFB fibers is as a substitution material in construction which usually the material is derived from non-renewable mining materials so that the number is increasingly limited. Therefore, it is necessary to study to know the performance of OPEFB fiber in making construction products especially concrete. In this case, the experiment was conducted using experimental method with variation of fiber addition by 0%, 10%, 15%, 20%, 25%, and 30%. Each specimen was tested by weight, slump value, compressive strength, tensile strength, elasticity and crack length. As the results, the variation of fibers addition by 10%, decrease of slump value is 7%, concrete weight is 3% and crack length is 8% while increase of the compressive strength is 2.7% and the modulus of elasticity is 33.3% but its tensile strength decreased insignificantly by 0.05% . Furthermore, the addition of fibers above 10% to 30% decreased compressive strength is still below 10% and tensile strength below 2% while the weight of concrete, slump value and crack length decreased. Therefore, the addition of 10% can replace the performance of concrete without fiber but the addition of above 10% can still be used on non-structural concrete.

scholarly journals Flammability, Tensile, and Morphological Properties of Oil Palm Empty Fruit Bunches Fiber/Pet Yarn-Reinforced Epoxy Fire Retardant Hybrid Polymer Composites

Polymers ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1282
Author(s):  
M.J. Suriani ◽  
Fathin Sakinah Mohd Radzi ◽  
R.A. Ilyas ◽  
Michal Petrů ◽  
S.M. Sapuan ◽  
...  
Keyword(s):  
Tensile Properties ◽  
Oil Palm ◽  
Natural Fiber ◽  
Fire Retardant ◽  
Epoxy Composites ◽  
Morphological Properties ◽  
Hybrid Polymer ◽  
Fiber Volume ◽  
Fiber Loading ◽  
Empty Fruit Bunches

Oil palm empty fruit bunches (OPEFB) fiber is a natural fiber that possesses many advantages, such as biodegradability, eco-friendly, and renewable nature. The effect of the OPEFB fiber loading reinforced fire retardant epoxy composites on flammability and tensile properties of the polymer biocomposites were investigated. The tests were carried out with four parameters, which were specimen A (constant), specimen B (20% of fiber), specimen C (35% of fiber), and specimen D (50% of fiber). The PET yarn and magnesium hydroxide were used as the reinforcement material and fire retardant agent, respectively. The results were obtained from several tests, which were the horizontal burning test, tensile test, and scanning electron microscopy (SEM). The result for the burning test showed that specimen B exhibited better flammability properties, which had the lowest average burning rate (11.47 mm/min). From the tensile strength, specimen A revealed the highest value of 10.79 N/mm2. For the SEM morphological test, increasing defects on the surface ruptured were observed that resulted in decreased tensile properties of the composites. It can be summarized that the flammability and tensile properties of OPEFB fiber reinforced fire retardant epoxy composites were reduced when the fiber volume contents were increased at the optimal loading of 20%, with the values of 11.47 mm/min and 4.29 KPa, respectively.

Activated carbons derived from oil palm empty-fruit bunches: Application to environmental problems

2007 ◽  
Vol 19 (1) ◽  
pp. 103-108 ◽  
Author(s):  
Md. Zahangir ALAM ◽  
Suleyman A. MUYIBI ◽  
Mariatul F. MANSOR ◽  
Radziah WAHID
Keyword(s):  
Oil Palm ◽  
Activated Carbons ◽  
Environmental Problems ◽  
Empty Fruit Bunches
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