scholarly journals Search for promising bacterial strains to create new biological preparations for increasing wheat yield

2020 ◽  
Author(s):  
L. S. Veselova ◽  
◽  
G. V. Mirskaya ◽  
Yu. V. Ostankova ◽  
T. A. Kuznetsova ◽  
...  
Keyword(s):  
Root Length ◽  
Wheat Yield ◽  
Bacillus Sp ◽  
Bacterial Strains ◽  
Active Components ◽  
Wheat Grains ◽  
Biological Products ◽  
Preliminary Results ◽  
Rrna Sequencing ◽  
16 S Rrna

The aim of this study is to search for promising strains of bacteria to create new biological products. New bacteria were isolated from wheat grains and identified by 16 S rRNA sequencing as Bacillus sp. L1 and Panaebacillus sp. L2. The cytokinin-producing bacteria Bacillussp.L 1 increased the length of the roots of plants by 70 %, the mass of roots by 17 %. N-fixing Panaebacillus sp. L2 increased the root length by 39 % and the mass by 27 %. The results of vegetation experiments on growing wheat of the ‘Leningradskaya 6’ variety under controlled conditions showed an increase in wheat productivity by 15.6 % when inoculated with Bacillus sp. L1 and by 31.2 % when inoculated with Panaebacillus sp. L2. Preliminary results of vegetative experiments indicate that Bacillus sp. L1 and Panaebacillus sp. L2 can be used as an active components of biological products to increase wheat productivity.

scholarly journals The fabrication of the complex bio-fertilizer for wheat cultivation based on collection bacteria of the PGPR group

2020 ◽  
Vol 21 (11) ◽  
Author(s):  
RAKHILYA AIPOVA ◽  
AIZHAN ABDYKADYROVA ◽  
DMITRY SILAYEV ◽  
ERKIN TAZABEKOVA ◽  
IRINA OSHERGINA ◽  
...  
Keyword(s):  
Wheat Yield ◽  
Practical Interest ◽  
Plant Growth Promoting Rhizobacteria ◽  
Phytopathogenic Fungi ◽  
Plant Diseases ◽  
Bacterial Strains ◽  
Biological Products ◽  
Wheat Cultivation ◽  
Plant Growth Promoting ◽  
Wheat Products

Abstract. Aipova R, Abdykadyrova A, Silayev D, Tazabekova E, Oshergina I, Ten E, Kurmanbaye A. 2020. The fabrication of the complex bio-fertilizer for wheat cultivation based on collection bacteria of the PGPR group. Biodiversitas 21: 5021-5028. The development of new types of biological products based on microbial complexes from local bacterial strains is a great theoretical and practical interest for agriculture. It can provide an opportunity for better preservation of the natural properties of the wheat products under extreme conditions. The aim of this study was to obtain and test a biological product to increase wheat productivity in northern Kazakhstan. Our data indicate the potential of Plant Growth-Promoting Rhizobacteria (PGPR) group bacteria for the development of biofertilizers and biopesticides. For instance, the bacteria B. mojavensis showed effectiveness in the experiments with the wheat (Astana-2 type). We observed an increase in wheat yield by 15% under conditions of artificial infection of crops with snow mold (by 2.5fold compared with the control). The results demonstrated that the B. mojavensis Lhv 97 strain can be used as an ingredient of biological products due to its activity against plant diseases caused by phytopathogenic fungi.

Functional characterization of PGPR and its identification through 16 S rRNA sequencing

2011 ◽  
Vol 3 (6) ◽  
pp. 47-50 ◽  
Author(s):  
Rashmin M Dhingani ◽  
◽  
Manoj V Parakhia ◽  
Rukam S Tomar ◽  
Bipin J Malviya ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Rrna Sequencing ◽  
16 S Rrna

scholarly journals Spectroscopic Study of the Molecular Structure of the New Hybrid with a Potential Two-Way Antibacterial Effect

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1442
Author(s):  
Dorota Kowalczuk ◽  
Agata Gładysz ◽  
Monika Pitucha ◽  
Daniel M. Kamiński ◽  
Agnieszka Barańska ◽  
...  
Keyword(s):  
Spectroscopic Study ◽  
Good Alternative ◽  
Structure Characterization ◽  
Resonance Spectroscopy ◽  
Spectroscopic Techniques ◽  
Bacterial Strains ◽  
Active Components ◽  
Hybrid Compound ◽  
Coordination Sites ◽  
Almost All

Bacterial strains become resistant to almost all classes of antibiotics, which makes it necessary to look for new substitutes. The non-absorbable ciprofloxacin–biguanide bismuth complex, used locally, may be a good alternative to a conventional therapy. The purpose of this study was to study the structure of the proposed ciprofloxacin (CIP) -bismuth(III)—chlorhexidine (CHX) composite (CIP-Bi-CHX). The spectroscopic techniques such as UV-VIS (ultraviolet-visible) spectroscopy, FTIR (Fourier-transform infrared) spectroscopy and NMR (Nuclear Magnetic Resonance) spectroscopy were used for structure characterization of the hybrid compound. The performed analysis confirmed the presence of the two active components—CIP and CHX and revealed the possible coordination sites of the ligands with bismuth ion in the metallo-organic structure. Spectroscopic study showed that the complexation between Bi(III) and CIP occurs through the carboxylate and ketone groups of the quinolone ring, while CHX combines with the central ion via the biguanide moieties.

scholarly journals Extraordinary Multi-Organismal Interactions Involving Bacteriophages, Bacteria, Fungi, and Rotifers: Quadruple Microbial Trophic Network in Water Droplets

2021 ◽  
Vol 22 (4) ◽  
pp. 2178
Author(s):  
Katarzyna Turnau ◽  
Edyta Fiałkowska ◽  
Rafał Ważny ◽  
Piotr Rozpądek ◽  
Grzegorz Tylko ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Stenotrophomonas Maltophilia ◽  
Laboratory Culture ◽  
Bacillus Sp ◽  
Water Droplets ◽  
Bacterial Strains ◽  
Molecular Analyses ◽  
Virus Like Particles ◽  
Trophic Network ◽  
Negative Effect

Our observations of predatory fungi trapping rotifers in activated sludge and laboratory culture allowed us to discover a complicated trophic network that includes predatory fungi armed with bacteria and bacteriophages and the rotifers they prey on. Such a network seems to be common in various habitats, although it remains mostly unknown due to its microscopic size. In this study, we isolated and identified fungi and bacteria from activated sludge. We also noticed abundant, virus-like particles in the environment. The fungus developed absorptive hyphae within the prey. The bacteria showed the ability to enter and exit from the hyphae (e.g., from the traps into the caught prey). Our observations indicate that the bacteria and the fungus share nutrients obtained from the rotifer. To narrow the range of bacterial strains isolated from the mycelium, the effects of bacteria supernatants and lysed bacteria were studied. Bacteria isolated from the fungus were capable of immobilizing the rotifer. The strongest negative effect on rotifer mobility was shown by a mixture of Bacillus sp. and Stenotrophomonas maltophilia. The involvement of bacteriophages in rotifer hunting was demonstrated based on molecular analyses and was discussed. The described case seems to be an extraordinary quadruple microbiological puzzle that has not been described and is still far from being understood.

scholarly journals Synthesis and Decontamination Effect on Chemical and Biological Agents of Benzoxonium-Like Salts

Toxics ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 222
Author(s):  
Aneta Markova ◽  
Michaela Hympanova ◽  
Marek Matula ◽  
Lukas Prchal ◽  
Radek Sleha ◽  
...  
Keyword(s):  
Biological Agents ◽  
Point Of View ◽  
Attractive Potential ◽  
Bacterial Strains ◽  
Active Components ◽  
Biological Warfare Agents ◽  
Warfare Agents ◽  
Single Compound ◽  
Safety Parameter ◽  
Low Toxicity

Benzoxonium chloride belongs to the group of quaternary ammonium salts, which have been widely used for decades as disinfectants because of their high efficacy, low toxicity, and thermal stability. In this study, we have prepared the C10-C18 set of benzoxonium-like salts to evaluate the effect of their chemical and biological decontamination capabilities. In particular, biocidal activity against a panel of bacterial strains including Staphylococcus aureus in biofilm form was screened. In addition, the most promising compounds were successfully tested against Francisella tularensis as a representative of potential biological warfare agents. From a point of view of chemical warfare protection, the efficiency of BOC-like compounds to degrade the organophosphate simulant fenitrothion was examined. Notwithstanding that no single compound with universal effectiveness was identified, a mixture of only two compounds from this group would be able to satisfactorily cover the proposed decontamination spectrum. In addition, the compounds were evaluated for their cytotoxicity as a basic safety parameter for potential use in practice. In summary, the dual effect on chemical and biological agents of benzoxonium-like salts offer attractive potential as active components of decontamination mixtures in the case of a terrorist threat or chemical or biological accidents.

scholarly journals Studies on decolorization of textile dye by using Pseudomonas and bacillus sp from the contaminated effluent soil samples of Kovilpatti, Thoothukudi district of Tamil Nadu

2019 ◽  
Vol 11 (1) ◽  
pp. 134-137 ◽  
Author(s):  
Kannan D ◽  
Renuga Devi ◽  
A. G. Murugesan ◽  
S. Rajan
Keyword(s):  
Contaminated Soil ◽  
Tamil Nadu ◽  
Soil Samples ◽  
Toxic Chemicals ◽  
Textile Dye ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Bacterial Strains ◽  
Textile Industries ◽  
Source Of Pollution

Textile industries releasing large amount of effluent which contains textile dyes and toxic chemicals and it is one of the major source of pollution also contaminating water bodies. To remove that, bacteria have been of great attention because of their ability to treat effluent. The present study was undertaken to exploit the ability of Pseudomonassp and Bacillus sp from dye contaminated soil samples for bioremediation for dye effluent. Among the bacterial strains used in the study. Pseudomonas sp emerged out to be most potent decolorizer in comparison to Bacillus sp with the degree of decolorization of 90.0 %. Thus, it was concluded that the Pseudomonas sp had highest color removing capacity from contaminated effluent soil samples. 

Isolation, Detection and Estimation of Various Amylase Producing Bacteria in Various Soil Samples

2021 ◽  
Vol 08 ◽  
Author(s):  
Mohd Amin Mir ◽  
Mohammad Waqar Ashraf ◽  
Altaf Hussain ◽  
Bilal Ahmad Mir
Keyword(s):  
Plant Extracts ◽  
Soil Samples ◽  
Bacillus Sp ◽  
Bacterial Strains ◽  
Cholesterol Levels ◽  
Microbiological Methods ◽  
Ethanolic Extracts ◽  
Microbial Strains ◽  
Litmus Test ◽  
Micro Organisms

Background: Soil is an ultimate source of all types of nutrients, which have both biological and non-biological importance. Studies are being carried out to isolate various types of micro-organisms from soil which have much more importance. So in the present study, amylase producing bacteria have been isolated from various soil samples. Aim: The isolation, identification, and estimation of various microbial strains for α-amylase enzyme production and then the inhibition of the growth of these microbial stains. Methods: The bacterial strains were isolated and then identified by various microbiological methods, including Gram’s staining method followed by several biochemical methods such as, litmus test, Gelatin test and Urea agar media and by viable cells. Results: Altogether, three microbial strains were identified from the soil samples in the concerned study. The concerned strains include- Shigella, Proteus and Bacillus, respectively. The concerned microbial strains were then analyzed for the amount of amylase enzyme and it had been found that Bacillus sp produces much more amount of amylase followed by Shigella sp, and lesser amylase enzyme producing activity was found in Proteus sp. The isolated bacteria were then analysed for inhibition of their growth by water and ethanolic extracts of Cuminum cyminuni. Among the extracts, it had been found that water extracts exhibited more inhibiting capacity than the ethanolic extracts. The study also revealed that among the bacterial strains, the Shigella sp got much more affected by the concerned plant extracts followed by Proteus sp and least inhibition was observed against the Bacillus sp. Conclusion: As per the above study, it is being concluded that - three amylase producing bacteria viz Shigella, proteus, bacillus sp were isolated from the soil samples. These isolated microbial strains could be used for the decomposition of cholesterol levels in human in addition to other microbial activity. These isolated bacterial could sometimes be averse therefore their growth could be stopped by various biological and chemical substances like Gentamicin and by various Plant extracts viz, Cuminum cyminuni Plant.

Research on Improvement of Nitrogenase Activity of Free-Living Nitrogen-Fixing Bacteria Using DBD Plasma

2013 ◽  
Vol 671-674 ◽  
pp. 2674-2678 ◽  
Author(s):  
Yan Yun Zhu ◽  
Xiao Li Zhu ◽  
Fang She Yang
Keyword(s):  
Rhizosphere Soil ◽  
Barrier Discharge ◽  
Nitrogenase Activity ◽  
Nitrogen Fixing ◽  
Bacterial Strains ◽  
Dbd Plasma ◽  
Nitrogen Fixing Bacteria ◽  
Free Living ◽  
Reduction Assay ◽  
Rrna Sequencing

Nitrogen-fixing bacteria were screened from the rhizosphere soil of plants in Shaanxi in China. 36 free-living nitrogen-fixing bacterial strains were isolated and their nitrogenase activity were determined by acetylene reduction assay (ARA), two strains named FLNB03 and FLNB09 with higher nitrogenase activity were isolated and identified by 16S rRNA sequencing. The datum showed that FLNB03 was similar to Acinetobacter and their similarity reached 99%, FLNB09 was similar to Agrobacterium sp. and their similarity reached 99%. Then both of them were treated using Dielectric Barrier Discharge (DBD) plasma for mutation and their mutants called FLNB03-2 and FLNB09-3 were obtained. The nitrogenase activity of FLNB03-2 was 0.61±0.10 nmol•107cfu-1•h-1, and that of FLNB09-3 was 0.40±0.05 nmol•107cfu-1•h-1, their nitrogenase activity increased by 22.00% and 14.29% than their original bacteria respectively. FLNB03-2 and FLNB09-3 might be used as microbial fertilizer.

Isolation, Characterization and Phylogenetic Analysis of Acidiphilium-Like Bacteria from Acid Mine Drainage

2007 ◽  
Vol 20-21 ◽  
pp. 473-476 ◽  
Author(s):  
Yan Fei Zhang ◽  
Yu Yang ◽  
Jian She Liu ◽  
Guan Zhou Qiu
Keyword(s):  
Acid Mine Drainage ◽  
Acidithiobacillus Ferrooxidans ◽  
Mine Drainage ◽  
Heterotrophic Bacteria ◽  
Bacterial Strains ◽  
Acid Mine ◽  
Autotrophic Bacteria ◽  
Rrna Sequencing ◽  
Relationship Of ◽  
Leaching Efficiency

The acidophilic heterotrophic bacteria Acidiphilium spp. were considered as a ministrant of Leptospirillum spp., Acidithiobacillus spp. and other autotrophic bacteria in acid mine drainage (AMD). 25 Acidiphilium-like bacterial strains were isolated from eight different mines in China and their physiology was characterized. Their marmatite-bioleaching ability has been investigated and compared with that of a mixed culture of Acidiphilium sp. and Acidithiobacillus ferrooxidans. The results indicated that some Acidiphilium-like strains exhibited a high marmatite-bioleaching ability, which was even higher than that of Acidithiobacillus ferrooxidans. The leaching efficiency was not higher in mixed than in pure culture. The phylogenetic relationship of the 25 Acidiphilium-like strains was analyzed by 16S rRNA sequencing, GyrB sequencing and rep-PCR genomic fingerprinting. The 25 strains and other eight species of Acidiphilium spp. were clustered into three groups.

scholarly journals Penghilangan Senyawa Fenol Oleh Bakteri Yang Diisolasi dari Area Pertambangan Minyak Bumi

2016 ◽  
Vol 17 (2) ◽  
pp. 126 ◽  
Author(s):  
Joko Prayitno
Keyword(s):  
Removal Efficiency ◽  
Contaminated Soil ◽  
Phenol Removal ◽  
Pseudomonas Sp ◽  
Bacillus Sp ◽  
Bacterial Strains

The aim of this research was to study the effectiveness of local bacterial strains from oil-contaminated soil to degrade phenol. The study consisted of two experiments, using six individual strains and using mix of strains. Bacterial strains used in the first experiment were 1.3, 3.3 dan 8.2.1 (Bacillus sp.), strain 3.2 (Propionibacterium), strain 3.4 (Pseudomonas sp.), and strain 8.1.2 (Enterobacter sp.).Bacterial strains used in the second experiment were mix of all six strains (K6) and mix of three strains (K3) consisted of  strain 3.4, 8.1.2 and 8.2.1 with the same ratio. The experiments were conducted in 100 mL Bushnell and Haas medium containing 300-400 ppm phenol for three days.Three strains (strain 3.4, 8.1.2, dan 8.2.1) had the highest phenol removal efficiency at day 3, i.e. 99-100%. COD values were decreased to 345-393 mg/L or 56-61.3% by those three strains. Mix culture K6 effectively removed phenol form the medium, but COD value decreased to only 56.7%. The fate of COD decrease was not the same as phenol removal by these strains (either in idividual or mix cultures), because phenol was degraded into intermediate compounds. 

Sign in / Sign up

Export Citation Format

Share Document