scholarly journals Genetic differentiation of dengue vector Aedes aegypti in the small geographical scale of Banyumas District, Indonesia based on Cytochrome Oxidase I

2021 ◽  
Vol 22 (2) ◽  
Author(s):  
Mohammed A. Mohammed ◽  
Agus Nuryanto ◽  
Endang Srimurni K Kusmintarsih
Keyword(s):  
Genetic Variation ◽  
Genetic Differentiation ◽  
Aedes Aegypti ◽  
Cytochrome Oxidase ◽  
Demographic History ◽  
Cytochrome Oxidase I ◽  
Dengue Vector ◽  
Central Java ◽  
Geographical Scale ◽  
Two Populations

Abstract. Mohammed MA, Nuryanto A, Kusmintarsih ES. 2021. Genetic differentiation of dengue vector Aedes aegypti in the small geographical scale of Banyumas District, Indonesia based on Cytochrome Oxidase I. Biodiversitas 22: 675-683. Aedes aegypti (Linnaeus, 1762) is a major vector of arboviruses. Currently, Ae. aegypti is spreading throughout the Banyumas District, Central Java, Indonesia and there is little information regarding the genetic variation of this species, yet the information is essential to develop effective vector control measures. The aims of this study was to evaluate the genetic differentiation of Ae. aegypti populations in South Purwokerto and Cilongok subdistricts. Mosquito larvae were collected using oviposition traps inside houses in South Purwokerto and Cilongok (Jastaba village). Twenty larvae of Ae. aegypti, ten from each location were identified and used for genetic analysis. Analysis of mitochondrial cytochrome Oxidase I (mtCOI) produced around 600 bp fragments, detecting ten haplotypes. The haplotype diversity and nucleotide diversity indices were higher in South Purwokerto samples. Tajima and Fu’s statistics indicated that the sampled populations were in genetic equilibrium. Indices of demographic history were fit for a spatial expansion model. Higher genetic variation was observed within the population compared to between the populations. Furthermore, strong genetic difference was detected between the two populations, with highly significant FS (p < 0.0001). Weak haplotype sharing occurred between the two populations assuming that the gene flow was facilitated by human transportation. All haplotypes were a cluster of single clades and closely related to haplotypes generated from previous studies in Central Java.

Molecular analysis of mitochondrial cytochrome oxidase I gene of Aedes aegypti L. mosquitoes

2020 ◽  
Vol 23 (1) ◽  
pp. 51-59 ◽  
Author(s):  
Ratnapal Gandhi ◽  
Kamlesh K. Yadav ◽  
Prabhakargouda B. Patil ◽  
Pankaj Bihani ◽  
Bharat Char ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Cytochrome Oxidase ◽  
Molecular Analysis ◽  
Cytochrome Oxidase I ◽  
Mitochondrial Cytochrome ◽  
Cytochrome Oxidase I Gene ◽  
Mitochondrial Cytochrome Oxidase ◽  
I Gene

scholarly journals The duck cytochrome oxidase I (COI) gene: Sequence and patterns analysis for potential barcoding tool

2018 ◽  
Vol 19 (3) ◽  
pp. 997-1003
Author(s):  
R. SUSANTI ◽  
RETNO SRI ISWARI ◽  
FIDIA FIBRIANA ◽  
INDRIAWATI INDRIAWATI
Keyword(s):  
Cytochrome Oxidase ◽  
Dna Barcoding ◽  
Cytochrome C Oxidase ◽  
Gene Sequence ◽  
Cytochrome Oxidase I ◽  
Coi Gene ◽  
Mitochondrial Cytochrome ◽  
Central Java ◽  
Synonym Substitution ◽  
Haplotype A

Susanti R, Iswari RS, Fibriana F, Indriawati. 2018. The duck cytochrome oxidase I (COI) gene: Sequence and patterns analysis for potential barcoding tool. Biodiversitas 19: 997-1003. The local duck DNA barcoding is still rarely conducted in Indonesia while DNA barcoding is extensively used as a tool of species identification and delineation tool. This study aimed to analyze the sequence and patterns of Central Javanese ducks mitochondrial cytochrome c oxidase subunit 1 (COI) gene. Feather samples of seven breeds of native duck were collected from traditional husbandries in Central Java. The samples were employed for DNA extraction and COI gene amplification. Five haplotypes were obtained from 35 samples, i.e., haplotype A, B, C, D, and E. Also, 9 variable sites with synonym substitution was detected in four nucleotides number 55, 61, 100, and 109; whereas five synonym substitutions were identified in the nucleotides number 36, 48, 51, 66, and 756. In conclusion, this study annotates that COI mtDNA gene is essential for local ducks barcoding system.

scholarly journals Aedes aegypti on Madeira Island (Portugal): genetic variation of a recently introduced dengue vector

2013 ◽  
Vol 108 (suppl 1) ◽  
pp. 3-10 ◽  
Author(s):  
Goncalo Seixas ◽  
Patricia Salgueiro ◽  
Ana Clara Silva ◽  
Melina Campos ◽  
Carine Spenassatto ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Aedes Aegypti ◽  
Dengue Vector ◽  
Madeira Island

Molecular Phylogenetics and Population Genetics of the Dengue Vector Aedes aegypti From the Arabian Peninsula

2021 ◽  
Author(s):  
Emad I M Khater ◽  
Farrukh Baig ◽  
Hany A Kamal ◽  
Jeffery R Powell ◽  
Amgad A Saleh
Keyword(s):  
Population Genetics ◽  
Genetic Variation ◽  
Aedes Aegypti ◽  
Molecular Phylogenetics ◽  
Arabian Peninsula ◽  
Neighbor Joining ◽  
Dengue Vector ◽  
Kingdom Of Saudi Arabia ◽  
Multiple Gene ◽  
Population Genetic Variation

Abstract Aedes aegypti mosquito is the principal dengue vector in the Kingdom of Saudi Arabia (KSA); however, no study has addressed its ecology and population structure yet. Therefore, we report on Ae. aegypti phylo- and population genetics using three DNA markers: COI, ND4, and rDNA-ITS2. Sampling the immature stages of Ae. aegypti revealed that water storage tanks (34.3% of habitats) were the most productive and contained 33% of immatures stages. Other important habitats included containers for wastewater drainage (including air-conditioning and water cooler trays) and containers associated with ornamentation. Shallow water leakage spots (2.7% of habitats, 8% of immatures) can be considered rare-but-epidemiologically-important containers. Neighbor-joining (NJ) phylogenetic analysis of Ae. aegypti identified 8, 14, and 9 haplotypes of COI, ND4, and ITS2, respectively, and revealed high levels of genetic variation in Ae. aegypti populations of KSA. Global distribution of haplotypes also indicated multiple gene introductions into these populations, with high levels of intra-population genetic variation and continuous gene exchange. The neutrality values indicated a deficiency of alleles and suggested that the KSA Ae. aegypti loci tested did not follow a neutral model of molecular evolution. Fst values and AMOVA indicated that most of the genetic variation in the KSA Ae. aegypti populations is due to intra- rather than inter-population differences. This is the first comprehensive report on the phylo- and population genetics of Ae. aegypti from the Arabian Peninsula. This information expands our understanding of the ecology and population dynamics of this important arboviral vector for informed control efforts.

Genetic and morphological differentiation among populations of the Rivoli’s Hummingbird (Eugenes fulgens) species complex (Aves: Trochilidae)

The Auk ◽  
2020 ◽  
Vol 137 (4) ◽  
Author(s):  
Luz E Zamudio-Beltrán ◽  
Juan Francisco Ornelas ◽  
Andreia Malpica ◽  
Blanca E Hernández-Baños
Keyword(s):  
Genetic Variation ◽  
Gene Flow ◽  
Genetic Differentiation ◽  
Species Complex ◽  
Ecological Niche Modeling ◽  
Demographic History ◽  
Morphological Differentiation ◽  
Last Interglacial ◽  
Haplotype Networks ◽  
Isthmus Of Tehuantepec

Abstract Genetic variation and phylogeographic studies have been crucial for understanding mechanisms of speciation. We analyzed genetic variation and phylogeography to reconstruct the demographic history of the Rivoli’s Hummingbird (Eugenes fulgens) species complex and also evaluated their morphological differentiation. This widely distributed species inhabits the highlands of Mexico and northern Central America, with 2 subspecies separated by the Isthmus of Tehuantepec (west: E. f. fulgens, east: E. f. viridiceps). We surveyed genetic variation in 2 mitochondrial DNA markers (mtDNA, with 129 individuals) and nuclear DNA (6 microsatellites, with 85 individuals). We also inferred the demographic history, estimated divergence times, and analyzed morphological variation using 470 vouchered specimens. We modeled the current potential distribution of the species using ecological niche modeling and projected it into the past to model the effects of the Pleistocene climatic cycles. Haplotype networks, pairwise FST comparisons, AMOVA, and morphological analysis revealed differences between geographically isolated populations separated by the Isthmus of Tehuantepec (IT; corresponding to the 2 recognized subspecies: fulgens and viridiceps), and by the Motagua-Polochic-Jocotán (MPJ) system fault. Demographic scenarios revealed a contraction in distribution during the last interglacial, and expansion during the Last Glacial Maximum (LGM) with little change since the LGM. Divergence between groups separated by the Isthmus of Tehuantepec ~59,600 yr ago occurred in the presence of gene flow, suggesting that the Isthmus of Tehuantepec is a semipermeable barrier to gene flow. STRUCTURE analyses of microsatellite data detected 3 genetically differentiated groups. Several results fit a model of recent lineage divergence, including a significant signal of genetic differentiation, demographic expansion, decreased gene flow from past to present, and northward expansion during the LGM and contraction during the interglacial periods. We conclude that the genetic differentiation of E. fulgens in the Madrean Pine-Oak Woodlands resulted from recent geographical isolation of populations separated by natural barriers (IT and MPJ).

scholarly journals Resistensi Malathion dan Aktivitas Enzim Esterase pada Populasi Nyamuk Aedes aegypti di Kabupaten Pekalongan

2018 ◽  
pp. 61-70
Author(s):  
Dyah Widiastuti ◽  
Bina Ikawati
Keyword(s):  
Enzyme Activity ◽  
Aedes Aegypti ◽  
Hemorrhagic Fever ◽  
Dengue Vector ◽  
Central Java ◽  
Biochemical Assays ◽  
Biochemical Mechanisms ◽  
Study Sites ◽  
F1 Generation ◽  
Dengue Vector Control

Pekalongan regency, Central Java (Indonesia) is one of area which experienced with Dengue Hemorrhagic Fever (DHF) incidence since many years ago. The extensive use of insecticides for dengue vector control has been carried out for long period in Pekalongan Regency. This fact stimulate the need to study the resistence status and  esterase enzyme avtivity of Aedes aegypti to Malathion. The research was conducted in April-November 2014. Insecticide susceptibility assays were performed on F1 generation of wild-caught adult female Ae. aegypti mosquitoes from Simbangkulon Village Buaran Sub district, Kedungwuni Barat Village Kedungwuni Sub dsitrict and Karangsari Village Karanganyar Sub district, with the diagnostic doses of malathion (0,8%). Esterase enzyme activity of these mosquitoes was tested biochemically to find out the mechanisms of resistance to Malathion. Mosquitoes mortality as a result of Malathion effect showed variation in range from 3.33 to 13.75% in all study sites. That result indicated that Ae.aegypti mosquitoes are resistant to Malathion in all study sites . Based on biochemical assays, there was an increase if esterase enztyme activity of Ae aegypti in all study sites.. Therefore, alpha esterase enzyme activity could be associated with the mechanisms which responsible to malathion resistance in Ae. aegypti in Pekalongan. The results of this study provided the information about the malathion resistance status of Ae. aegypti in Pekalongan, and biochemical mechanisms which essential for monitoring the insecticide resistance in this area.  

scholarly journals Insecticide-Driven Patterns of Genetic Variation in the Dengue Vector Aedes aegypti in Martinique Island

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e77857 ◽  
Author(s):  
Sébastien Marcombe ◽  
Margot Paris ◽  
Christophe Paupy ◽  
Charline Bringuier ◽  
André Yebakima ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Aedes Aegypti ◽  
Dengue Vector
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