scholarly journals Inhibition of a-amylase and a-glucosidase activities in vitro by extracts of selected medicinal plants

2021 ◽  
Vol 22 (3) ◽  
Author(s):  
Deepak Pant ◽  
BIVA ARYAL ◽  
DAMA PUN ◽  
SUSHILA SHARMA ◽  
GIRI PRASAD JOSHI
Keyword(s):  
Medicinal Plants ◽  
Inhibitory Effect ◽  
Scientific Basis ◽  
Aqueous Extracts ◽  
Extraction Solvent ◽  
Methanolic Extracts ◽  
Treatment Of Diabetes ◽  
High Degree ◽  
Very High

Abstract. Pant DR, Aryal B, Pun D, Sharma S, Joshi GP. 2021. Inhibition of a-amylase and a-glucosidase activities in vitro by extracts of selected medicinal plants. Biodiversitas 22: 1187-1193. Several medicinal plants are being used traditionally in the treatment/management of Diabetes Mellitus (DM). The present work focuses on experimental verification of antidiabetic potential of different medicinal plants that have been reported to be used traditionally to manage DM. Aqueous and methanolic extracts of 12 species of plants were studied for their inhibitory effect on the activities of a-amylase and a-glucosidase, two key enzymes of carbohydrate metabolism. The extracts of all the species showed very high degree (nearly 90% or above) of inhibition of α-amylase irrespective of the extraction solvent. The aqueous extracts of Asterella wallichiana, however, showed only 63.60% inhibition of a-amylase. Except for Asterella wallichiana, the percentage inhibition of a-amylase in aqueous and methanolic solution in all the species tested were almost similar. The percentage inhibition of a-glucosidase was lower than that of a-amylase for all the species in both types of extraction medium. The highest percentage inhibition of a-glucosidase (81.13±1.36 %) was found in methanolic extracts of Rhuschinensis. The inhibition of a-glucosidase was much higher in methanolic extracts than in aqueous extracts for all the species tested. High degree of inhibition of a-amylase activity in vitro by the extracts of all the species tested provides scientific basis for using these plants in the management/treatment of diabetes in traditional medicine.

scholarly journals Antiplasmodial and Cytotoxic Activities of Extracts of Selected Medicinal Plants Used to Treat Malaria in Embu County, Kenya

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Bibianne Waiganjo ◽  
Gervason Moriasi ◽  
Jared Onyancha ◽  
Nelson Elias ◽  
Francis Muregi
Keyword(s):  
Side Effects ◽  
Medicinal Plants ◽  
Stem Bark ◽  
Antiplasmodial Activity ◽  
Chloroquine Resistance ◽  
Cytotoxic Activities ◽  
Aqueous Extracts ◽  
Methanolic Extracts

Malaria is a deadly disease caused by a protozoan parasite whose mode of transmission is through a female Anopheles mosquito. It affects persons of all ages; however, pregnant mothers, young children, and the elderly suffer the most due to their dwindled immune state. The currently prescribed antimalarial drugs have been associated with adverse side effects ranging from intolerance to toxicity. Furthermore, the costs associated with conventional approach of managing malaria are arguably high especially for persons living in low-income countries, hence the need for alternative and complementary approaches. Medicinal plants offer a viable alternative because of their few associated side effects, are arguably cheaper, and are easily accessible. Based on the fact that studies involving antimalarial medicinal plants as potential sources of efficacious and cost-effective pharmacotherapies are far between, this research was designed to investigate antiplasmodial and cytotoxic activities of organic and aqueous extracts of selected plants used by Embu traditional medicine practitioners to treat malaria. The studied plants included Erythrina abyssinica (stem bark), Schkuhria pinnata (whole plant), Sterculia africana (stem bark), Terminalia brownii (leaves), Zanthoxylum chalybeum (leaves), Leonotis mollissima (leaves), Carissa edulis (leaves), Tithonia diversifolia (leaves and flowers), and Senna didymobotrya (leaves and pods). In vitro antiplasmodial activity studies of organic and water extracts were carried out against chloroquine-sensitive (D6) and chloroquine-resistance (W2) strains of Plasmodium falciparum. In vivo antiplasmodial studies were done by Peter’s four-day suppression test to test for their in vivo antimalarial activity against P. berghei. Finally, cytotoxic effects and safety of the studied plant extracts were evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) rapid calorimetric assay technique. The water and methanolic extracts of T. brownii and S. africana and dichloromethane extracts of E. abyssinica, S. pinnata, and T. diversifolia leaves revealed high in vitro antiplasmodial activities (IC50≤10 μg/ml). Further, moderate in vivo antimalarial activities were observed for water and methanolic extracts of L. mollissima and S. africana and for dichloromethane extracts of E. abyssinica and T. diversifolia leaves. In this study, aqueous extracts of T. brownii and S. africana demonstrated high antiplasmodial activity and high selectivity indices values (SI≥10) and were found to be safe. It was concluded that T. brownii and S. africana aqueous extracts were potent antiplasmodial agents. Further focused studies geared towards isolation of active constituents and determination of in vivo toxicities to ascertain their safety are warranted.

Control of Green Rot Fungus of Arachis hypogaea L. in Orage Using Plant Extracts

2016 ◽  
Vol 58 ◽  
pp. 77-84
Author(s):  
Etim Johnson Umana ◽  
Patrick Ishoro Akwaji ◽  
Emmanuel Michael Ekpenyong ◽  
Penninah Ime Hanson
Keyword(s):  
Plant Extracts ◽  
Inhibitory Effect ◽  
Aqueous Extracts ◽  
Phytochemical Content ◽  
Fruit Extracts ◽  
Extraction Solvent ◽  
Effective Inhibition ◽  
Ethanolic Extracts ◽  
The Media

The antifungal activity of the ethanolic and aqueous fruit extracts of Zingiberofficinale, Tetrapleuratetraptera, Garcinia kola and Cola nitida on the green rot fungus (Penicillium sp.) isolated from infected groundnut (Arachishypogaea) seeds in storage were investigated in vitro. Various concentrations of the aqueous and ethanolic fruit extract ranging from 5g/100ml, 10g/100ml, and 15g/100ml were separately added to Potato Dextrose Agar (PDA) media. The fungus was inoculated into the media and incubated for seven days. Results of the in vitro studies showed that the ethanolic extracts had a significant inhibitory effect (p<0.05) on the radial growth of Penicillium sp. at all levels of concentration tested. Complete inhibition of the fungus was at 15g/100ml of ethanolic extracts of Garcinia kola and Tetrapleuratetraptera. The aqueous extracts showed less inhibitory effect as compared to the ethanolic extracts. The effective inhibition of ethanolic extracts as compared to the aqueous extracts may be attributed to the efficiency of the extraction solvent on the phytochemical content of the plant extracts. The application of botanical extracts for disease management could be less expensive, easily available, non-polluting and eco-friendly. The ethanolic fruit extracts of Garcinia kola and Tetrapleuratetraptera at the higher concentrations can be produced in large quantities and used as spray in controlling the green rot fungus of Arachishypogaea in storage.

scholarly journals Inhibitory Potential of Five Traditionally Used Native Antidiabetic Medicinal Plants onα-Amylase,α-Glucosidase, Glucose Entrapment, and Amylolysis KineticsIn Vitro

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Carene M. N. Picot ◽  
A. Hussein Subratty ◽  
M. Fawzi Mahomoodally
Keyword(s):  
Medicinal Plants ◽  
Mixed Type ◽  
Diabetes Management ◽  
Kinetic Studies ◽  
Aqueous Extracts ◽  
Methanolic Extracts ◽  
Glucose Diffusion ◽  
Dependent Activity ◽  
Inhibitory Potential

Five traditionally used antidiabetic native medicinal plants of Mauritius, namely,Stillingia lineata(SL),Faujasiopsis flexuosa(FF),Erythroxylum laurifolium(EL),Elaeodendron orientale(EO), andAntidesma madagascariensis(AM), were studied for possibleα-amylase andα-glucosidase inhibitory property, glucose entrapment, and amylolysis kineticsin vitro. Only methanolic extracts of EL, EO, and AM (7472.92±5.99,1745.58±31.66, and2222.96±13.69 μg/mL, resp.) were found to significantly (P<0.05) inhibitα-amylase and were comparable to acarbose. EL, EO, AM, and SL extracts (5000 μg/mL) were found to significantly (P<0.05) inhibitα-glucosidase (between87.41±3.31and96.87±1.37% inhibition). Enzyme kinetic studies showed an uncompetitive and mixed type of inhibition. Extracts showed significant (P<0.05) glucose entrapment capacities (8 to 29% glucose diffusion retardation index (GDRI)), with SL being more active (29% GDRI) and showing concentration-dependent activity (29, 26, 21, 14, and 5%, resp.). Amylolysis kinetic studies showed that methanolic extracts were more potent inhibitors ofα-amylase compared to aqueous extracts and possessed glucose entrapment properties. Our findings tend to provide justification for the hypoglycaemic action of these medicinal plants which has opened novel avenues for the development of new phytopharmaceuticals geared towards diabetes management.

scholarly journals Various Extracts of Some Medicinal Plants as Inhibitors for Beta-lactamase Activity

2021 ◽  
Vol 18 (1) ◽  
pp. 0047
Author(s):  
Huda S. A. Al-Hayanni ◽  
Hamed El-Shora
Keyword(s):  
Medicinal Plants ◽  
Klebsiella Pneumoniae ◽  
Agar Plate ◽  
Eucalyptus Camaldulensis ◽  
Inhibitory Effect ◽  
Individual Plant ◽  
Aqueous Extracts ◽  
Schinus Terebinthifolius ◽  
Acetone Extracts

The inhibitory effect of acetone, ethanol, and aqueous extracts of ten medicinal plants on β-lactamase from Staphylococcus sciuri and Klebsiella pneumoniae was investigated in vitro by starch-iodine agar plate method. The results revealed the success of starch-iodine method for the detection of the inhibition of β-lactamase activity by the various extracts of each individual plant. The acetone extracts of Catharanthus roseus, Eucalyptus camaldulensis, and Schinus terebinthifolius induced an inhibitory effect on β-lactamase from Staphylococcus sciuri. On the other hand, acetone extracts from only Eucalyptus camaldulensis, and Schinus terebinthifolius expressed strong inhibitory effect on β-lactamase from Klebsiella pneumoniae. The acetone extracts expressed the highest inhibition for β-lactamases activity compared to ethanolic and aqueous extracts which exhibited appreciable inhibitory effect. β-lactamase from S. sciuri was inhibited by extracts from C. roseus, E. camaldulensis and S. terebinthifolius whereas β-lactamase from K. pneumoniae was inhibited only by extracts from E. camaldulensis and S. terebinthifolius.

scholarly journals Anti-inflammatory, anti-oxidant and wound-healing properties of selected South Africa medicinal plants

2017 ◽  
Author(s):  
◽  
Nonkululeko Betty Mzindle
Keyword(s):  
Wound Healing ◽  
Medicinal Plants ◽  
Migration Rate ◽  
Radical Scavenging ◽  
Fibroblast Cells ◽  
Hibiscus Sabdariffa ◽  
Aqueous Extracts ◽  
Methanolic Extracts ◽  
Anti Inflammatory

South Africa has a wide range of medicinal plants that are used traditionally by black Zulu South Africans for the treatment of a range of illnesses, including inflammatory ailments; disease conditions caused by oxidative stress and wound healing. It has been indicated that bioactive compounds isolated from plants contribute to their anti-inflammatory, antioxidant and wound healing properties; hence, herbal remedies have been widely used traditionally in many countries in the management and treatment of wounds. Inflammation is the main condition that relates to a variety of diseases affecting most of the world’s population. It is the body’s immune response to infection and injury and is induced by the release of pro-inflammatory mediator’s —prostaglandins and leukotrienes—following wound occurrence. Wounds result in disruption of living tissue caused by oxidative stress. Anti-inflammatory agents, antioxidants, and antimicrobials play an important role in the wound healing process and they prevent aggravated wound conditions.Controlling inflammation during wound repair is important to minimize any additional complications that may result; hence, chemical agents such as non-steroidal anti-inflammatory drugs (NSAIDS), synthetic antioxidantsand steroids are frequently used. These drugs block the enzymes that are responsible for prostaglandin synthesis in inflammation, react with free radicals thereby interfering with oxidation process as a result affect one or more phases of wound healing. The use of these drugs, however, has been limited as they can cause detrimental side effects when used over long periods of time.There is, consequently, a need to find alternative natural therapeutic drugs. Studies on medicinal plants confirmed that herbal drugs exhibit fewer side effects in comparison with chemical agents and are more cost-effective.Thus the aim of this study was to investigate South African medicinal plants, for anti-inflammatory, antioxidant and wound healing properties. Dissolved extracts of thirty-eight medicinal plants were evaluated for theiranti-inflammatory activity using the 5-lipoxygenase assay as well as free radical scavenging activity using the 1; 1-diphenyl-2-picrylhydrazyl (DPPH) assay.Their safety was evaluated using brine shrimp lethality assay. Proliferation and viability of fibroblast cells was determined by the3-(4, 5-dimethylthiazolyl)-2, 5-diphenyltetrazolium bromide(MTT) assay furthermore a scratch wound assay was used to study the properties of wound healing in vitro and to confirm the anti-inflammatory activities of the dissolved extracts. Migration rate was evaluated quantitatively by an image analyzer. Methanol was chosen for extraction because it completely dissolves extracts. Anova was used for statistical analysis. Almost all aqueous extracts were found to be effective in inhibiting lipoxygenase enzyme when compared to nordihydroguaiaretic acid (NDGA). Aqueous extracts exhibited remarkably high percentage inhibition of lipoxygenase with most above 100% when compared to methanolic extracts. Amaranthus dubius and Portulaca oleracea were found to have good biological activities in the inhibition of 5-lipoxygenase enzymes when compared to the other plants. However, Galinsoga parviflora and Syzygium cordatumwere least effective in inhibiting enzyme activity with percentages as low as -2% and 34% respectively. Percentage inhibitions for methanolic extracts were lower than that of aqueous extracts. Amaranthus spinosus had the highest percentage inhibition among all the methanolic extracts andGalinsoga parviflorahad the lowest. The methanolic plant extracts were found to be more effective in scavenging DPPH free radicals than the corresponding aqueous extracts. All the methanolic extracts exhibited free radical scavenging ability in the range of 60%–104%. Asystasia gangetica, Ficus sur, Heteropyxis natalensis, Hibiscus sabdariffa, Pelargonium sp. showed notably higher scavenging abilities, ranging from 101%–104% compared to Rutin. Methanolic extracts of Heteropyxis natalensis and Hibiscus sabdariffa exhibited scavenging ability even at the lowest concentration of 10μg/ml. Furthermore, aqueous extracts displayed remarkably lower activities than methanolic extracts with thirty-one extracts having a scavenging capacity ranging from 22%—59%. None of the extracts were found to be detrimental to brine shrimp. Almost all the extracts were shown to stimulate the growth of fibroblast cells except the methanolic extract of Solanum nodiflorum, which was shown to be killing the cells at high concentrations with a percentage viability of 46%.As the concentration decreased, however, the viability of cells with this extract increased to 143%. An increase in the number of fibroblast cells was observed in the scratched area of the treated cells and a significant migration rate was also noted with some of the extracts. Aqueous extracts of Sonchus oleraceus (86%), Justicia flava (85%) and Dichrostachys cinerea (85%) and methanolic extracts of Senna occidentalis and Hibiscus sabdariffa were found to have the highest migration rate compared to untreated cells that served as a control. No cell migration was observed with methanolic extract of Solanum nodiflorum.Instead, the extract was found to be toxic to the cells. Some of the plants evaluated in this study have been studied for either anti-inflammatory, antioxidantand wound healing properties in vivo, however, no work has been conducted to demonstrate a correlation between anti-inflammatory, antioxidant and wound healing properties of plant species in vitro. The current study was, therefore, conducted to review medicinal herbs considered as anti-inflammatory, antioxidants and wound healing agents as well as collecting evidence for their effectiveness and pharmacological mechanisms in modern science. In the plant species investigated Amaranthus dubius, Asystasia gangetica, Bidens pilosa, Buddleja saligna, Carpobrotus dimidiatus, Chenopodium album, Dichrostachys cinerea, Emex australis, Ficus sur, Guilleminea densa, Hibiscus sabdariffa, Physalis viscose, Syzygium cordatum, Taraxacum officinale and Tulbaghia violacea demonstrated good anti-inflammatory and wound healing properties.In conclusion the results from this study demonstrated promising anti-inflammatory and antioxidantactivities as well as wound healing properties,furthermoreit was aslo shown that the plant extracts were not toxic to the cells hencethis suggested that the plants investigated, can be used as substitutes or to formulate wound healing agents that are safe to use in primary healthcare.

IN VITRO STUDIES OF SOME EDIBLE SPICES ON PANCREATIC LIPASE INHIBITORY ACTIVITY

INDIAN DRUGS ◽  
2017 ◽  
Vol 54 (02) ◽  
pp. 62-68
Author(s):  
S Mhatre ◽  
◽  
A. Bhagit ◽  
R. P Yadav
Keyword(s):  
Pancreatic Lipase ◽  
Inhibitory Activity ◽  
Inhibitory Effect ◽  
Syzygium Aromaticum ◽  
High Potency ◽  
Good Source ◽  
Zanthoxylum Armatum ◽  
Methanolic Extracts ◽  
Cinnamomum Tamala

Pancreatic lipase inhibitory effect of some edible spices in light of percent inhibition, efficacy, reversibility/ irreversibility and effect of pH on inhibition is presented here. Lipase inhibitory activities of methanolic extracts of eighteen spices were evaluated. Extracts of Zanthoxylum armatum, Cinnamomum tamala, Syzygium aromaticum and Myristica fragrans were considered to be of high potency in synthetic substrate assay. Only Syzygium aromaticum showed high potency in natural substrate based lipase assay. Zanthoxylum armatum extract displayed lowest IC50 of 9.0 μg/mL. On dialysis, all extracts lost their lipase inhibitory activity indicating reversible nature of inhibition. pH significantly affected the performance of spice extracts during inhibition of pancreatic lipase. Most of the extracts lost their pancreatic lipase inhibitory activity at pH 3.0 with the exception of Brassica nigra and Cinnamomum tamala. Results showed spice are good source of pancreatic lipase inhibitor and its potential as drug for obesity can be explored by addressing various issues.

Antimycobacterial screening of traditional medicinal plants using the microplate resazurin assay

2010 ◽  
Vol 56 (6) ◽  
pp. 487-494 ◽  
Author(s):  
Duncan Webster ◽  
Timothy D.G. Lee ◽  
Jill Moore ◽  
Tracy Manning ◽  
Dennis Kunimoto ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
First Nations ◽  
Antimycobacterial Activity ◽  
Multidrug Resistant ◽  
Positive Control ◽  
Health Concern ◽  
Acorus Calamus ◽  
Aqueous Extracts ◽  
Resazurin Assay

Multidrug-resistant Mycobacterium tuberculosis strains have rapidly become a global health concern. North American First Nations communities have used traditional medicines for generations to treat many pulmonary infections. In this study, we evaluated the antimycobacterial activity of 5 medicinal plants traditionally used as general therapeutics for pulmonary illnesses and specifically as treatments for tuberculosis. Aqueous extracts of Aralia nudicaulis , Symplocarpus foetidus , Heracleum maximum , Juniperus communis, and Acorus calamus were screened for antimycobacterial activity against Bacillus Calmette–Guérin, Mycobacterium avium, and M. tuberculosis H37Ra using the colorimetric microplate resazurin assay. Extracts of Acorus calamus and H. maximum root demonstrated significant antimycobacterial activity comparable to that of the rifampin control (2 µg/mL). Evaluation of the cytotoxicity of these 2 extracts using the MTT assay also showed that the extracts were less toxic to 3 human cell lines than was the DMSO positive control. This study demonstrates that aqueous extracts of the roots of H. maximum and Acorus calamus possess strong in vitro antimycobacterial activity, validates traditional knowledge, and provides potential for the development of urgently needed novel antituberculous therapeutics.

scholarly journals In Vitro Antibacterial and Antifungal Activity and Datura Innoxia Extracts

2014 ◽  
Vol 3 (3) ◽  
pp. 173-185
Author(s):  
Adam IY Shama ◽  
YM Abd-Kreem ◽  
AA Fadowa ◽  
RM Samar ◽  
MK Sabahelkhier
Keyword(s):  
Antimicrobial Activity ◽  
Plant Extracts ◽  
Antifungal Drugs ◽  
Diffusion Method ◽  
Antibacterial Drug ◽  
Aqueous Extracts ◽  
Datura Innoxia ◽  
Methanolic Extracts ◽  
Leaves And Roots

The aim of this study was evaluated the Antimicrobial Activity of extraction of Datura innoxia (Seeds, leaves and roots). Datura innoxia Seeds, leaves and roots were collected to examine their antimicrobial activity. Extracts of different parts of the plant were tested against standard microorganisms by using the agar- well diffusion method. Extracts of methanol, and aqueous of seeds, leaves and roots were prepared and tested against four types of bacteria namely: Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Proteus vulgaris and two types of fungi namely: Aspergillus niger and Candida albicans. The methanolic and aqueous extracts of leaves showed high activities against fungi (A. niger) and less effect on the all bacteria. The methanolic extracts of seeds showed high activities against all organisms except fungi (C. albicanas), while the aqueous extracts of seeds showed no activity on the bacteria. All organisms were examined against known standard antibiotics and then compare the results of plant extracts with standard antibiotics. The results indicated that the antibacterial drug is less active than the plant extracts, while the antifungal drugs are more active than the plant extracts. DOI: http://dx.doi.org/10.3126/ije.v3i3.11077 International Journal of Environment Vol.3(3) 2014: 173-185

Inhibitory effect of aqueous extracts of Centaurea solstitialis subsp. schouwii on seed germination and growth of Sulla coronaria

Botany ◽  
2020 ◽  
Vol 98 (5) ◽  
pp. 273-281
Author(s):  
Chadlia Hachani ◽  
Mohammed S. Lamhamedi ◽  
Mejda Abassi ◽  
Zoubeir Béjaoui
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Inhibitory Effect ◽  
Centaurea Solstitialis ◽  
Aqueous Extracts ◽  
Linear Relationships ◽  
Allelopathic Effects ◽  
Anthropogenic Threats ◽  
Sulla Coronaria

Biodiversity has been confronted with anthropogenic threats and several natural threats such as biological invasions. The success of these invasions involves phytotoxic products released by invasive plants that can exhibit allelopathic effects on target species. Thus, aqueous extracts from different parts of the Mediterranean yellow star-thistle [Centaurea solstitialis subsp. schouwii (DC.) Gugler], were tested for their allelopathic effects on seed germination and seedling growth of Sulla coronaria (L.). Bioassays were conducted in vitro to test the effects of the aqueous extracts of shoot, basal and root parts of C. solstitialis subsp. schouwii at two different concentrations (50 g·L−1 and 10 g·L−1). The concentrations of total polyphenols, flavonoids, and tannins of the extracts were also evaluated. Our results showed inhibitory effects on the germination and seedling growth of S. coronaria seedlings, particularly with the extract form the basal part, reaching 84%. This study confirms the linear relationships between the allelopathic effects of C. solstitialis subsp. schouwii and the polyphenol and flavonoid contents. However, further experiments are needed under field conditions to confirm the results obtained under laboratory conditions.

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