Fluorescent nanodiamonds for characterization of nonlinear microscopy systems

2021 ◽  
Author(s):  
Mantas Zurauskas ◽  
Aneesh Alex ◽  
Jaena Park ◽  
STEVE HOOD ◽  
Stephen Boppart
Keyword(s):  
Nonlinear Microscopy ◽  
Fluorescent Nanodiamonds

3D imaging and characterization of microlenses and microlens arrays using nonlinear microscopy

2013 ◽  
Vol 46 (19) ◽  
pp. 195101 ◽  
Author(s):  
Aleksandar J Krmpot ◽  
George J Tserevelakis ◽  
Branka D Murić ◽  
George Filippidis ◽  
Dejan V Pantelić
Keyword(s):  
3D Imaging ◽  
Nonlinear Microscopy ◽  
Microlens Arrays

Preparation and characterization of green fluorescent nanodiamonds for biological applications

2009 ◽  
Vol 18 (2-3) ◽  
pp. 567-573 ◽  
Author(s):  
Tse-Luen Wee ◽  
Yi-Wen Mau ◽  
Chia-Yi Fang ◽  
Hsiang-Ling Hsu ◽  
Chau-Chung Han ◽  
...  
Keyword(s):  
Biological Applications ◽  
Fluorescent Nanodiamonds ◽  
Green Fluorescent

Characterization of third-degree burned skin by nonlinear microscopy technique

2011 ◽  
Author(s):  
Moisés O. dos Santos ◽  
Vitor B. Pelegati ◽  
Carlos L. Cesar ◽  
Paulo R. Correa ◽  
Telma Maria T. Zorn ◽  
...  
Keyword(s):  
Nonlinear Microscopy ◽  
Microscopy Technique ◽  
Burned Skin

scholarly journals Thermometric Characterization of Fluorescent Nanodiamonds Suitable for Biomedical Applications

2021 ◽  
Vol 11 (9) ◽  
pp. 4065
Author(s):  
Francisco Pedroza-Montero ◽  
Karla Santacruz-Gómez ◽  
Mónica Acosta-Elías ◽  
Erika Silva-Campa ◽  
Diana Meza-Figueroa ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Data Representation ◽  
Colour Centres ◽  
High Pressure High Temperature ◽  
Aqueous Environments ◽  
Fluorescent Nanodiamonds ◽  
Low Cytotoxicity ◽  
Scientific Fields ◽  
Potential Use

Nanodiamonds have been studied for several biomedical applications due to their inherent biocompatibility and low cytotoxicity. Recent investigations have shown perspectives in using fluorescent nanodiamonds as nanothermometers because of their optical properties’ dependence on temperature. Easy and accurate localized temperature sensing is essential in a wide variety of scientific fields. Our work demonstrated how the fluorescence spectrum of high-pressure high-temperature fluorescent nanodiamonds of three different sizes: 35 nm, 70 nm and 100 nm, changes with temperature within an important biological temperature range (25 °C to 60 °C). Taking advantage of this phenomenon, we obtained nanothermic scales (NS) from the zero phonon lines (ZPL) of the NV0 and NV− colour centres. In particular, the 100 nm-sized features the more intense fluorescence spectra whose linear dependence with temperature achieved 0.98 R2 data representation values for both NV0 and NV−. This model predicts temperature for all used nanodiamonds with sensitivities ranging from 5.73% °C−1 to 6.994% °C−1 (NV0) and from 4.14% °C−1 to 6.475% °C−1 (NV−). Furthermore, the non-cytotoxic interaction with HeLa cells tested in our study enables the potential use of fluorescence nanodiamonds to measure temperatures in similar nano and microcellular aqueous environments with a simple spectroscopic setup.

Morphological Characterization of Mycobacteriophage R1

1974 ◽  
Vol 32 ◽  
pp. 254-255
Author(s):  
B. L. Soloff ◽  
T. A. Rado
Keyword(s):  
Carbon Source ◽  
Stationary Phase ◽  
Growth Phase ◽  
Minimal Medium ◽  
Morphological Characterization ◽  
Logarithmic Growth Phase ◽  
Complete Lysis ◽  
Lysogenic Culture ◽  
Logarithmic Growth

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.

AEM analysis of crystallization in Ti-based amorphous alloys

1983 ◽  
Vol 41 ◽  
pp. 270-271
Author(s):  
A.R. Pelton ◽  
A.F. Marshall ◽  
Y.S. Lee
Keyword(s):  
Magnetic Properties ◽  
Amorphous Alloys ◽  
Amorphous Materials ◽  
Isothermal Annealing ◽  
Amorphous Matrix ◽  
Nucleation And Growth ◽  
Current Interest ◽  
Amorphous Films ◽  
Electrical And Magnetic Properties

Amorphous materials are of current interest due to their desirable mechanical, electrical and magnetic properties. Furthermore, crystallizing amorphous alloys provides an avenue for discerning sequential and competitive phases thus allowing access to otherwise inaccessible crystalline structures. Previous studies have shown the benefits of using AEM to determine crystal structures and compositions of partially crystallized alloys. The present paper will discuss the AEM characterization of crystallized Cu-Ti and Ni-Ti amorphous films.Cu60Ti40: The amorphous alloy Cu60Ti40, when continuously heated, forms a simple intermediate, macrocrystalline phase which then transforms to the ordered, equilibrium Cu3Ti2 phase. However, contrary to what one would expect from kinetic considerations, isothermal annealing below the isochronal crystallization temperature results in direct nucleation and growth of Cu3Ti2 from the amorphous matrix.

Characterization of Coherent, Ordered Precipitates in Nickel-Base Alloys

1973 ◽  
Vol 31 ◽  
pp. 144-145
Author(s):  
B. H. Kear ◽  
J. M. Oblak
Keyword(s):  
Stable Phase ◽  
Nickel Base Superalloy ◽  
Alloy 718 ◽  
Commercial Alloy ◽  
Precipitate Morphology ◽  
Continuous Precipitation ◽  
Nickel Base ◽  
Base Superalloy ◽  
Strengthening Precipitate

A nickel-base superalloy is essentially a Ni/Cr solid solution hardened by additions of Al (Ti, Nb, etc.) to precipitate a coherent, ordered phase. In most commercial alloy systems, e.g. B-1900, IN-100 and Mar-M200, the stable precipitate is Ni3 (Al,Ti) γ′, with an LI2structure. In A lloy 901 the normal precipitate is metastable Nis Ti3 γ′ ; the stable phase is a hexagonal Do2 4 structure. In Alloy 718 the strengthening precipitate is metastable γ″, which has a body-centered tetragonal D022 structure.Precipitate MorphologyIn most systems the ordered γ′ phase forms by a continuous precipitation re-action, which gives rise to a uniform intragranular dispersion of precipitate particles. For zero γ/γ′ misfit, the γ′ precipitates assume a spheroidal.

The Use of Advanced Analytical Techniques for Characterization of the Chemical, Physical, and Crystallographic Nature of a Surface

1973 ◽  
Vol 31 ◽  
pp. 132-133 ◽  
Author(s):  
R. E. Herfert
Keyword(s):  
Surface Characterization ◽  
Analytical Techniques ◽  
X Ray Diffraction ◽  
Depth Of Penetration ◽  
X Ray ◽  
The Past ◽  
Transmission Electron ◽  
Scanning Electron

Studies of the nature of a surface, either metallic or nonmetallic, in the past, have been limited to the instrumentation available for these measurements. In the past, optical microscopy, replica transmission electron microscopy, electron or X-ray diffraction and optical or X-ray spectroscopy have provided the means of surface characterization. Actually, some of these techniques are not purely surface; the depth of penetration may be a few thousands of an inch. Within the last five years, instrumentation has been made available which now makes it practical for use to study the outer few 100A of layers and characterize it completely from a chemical, physical, and crystallographic standpoint. The scanning electron microscope (SEM) provides a means of viewing the surface of a material in situ to magnifications as high as 250,000X.

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