scholarly journals Sylvatic dengue virus type 4 in Aedes aegypti and Aedes albopictus mosquitoes in an urban setting in Peninsular Malaysia

2019 ◽  
Vol 13 (11) ◽  
pp. e0007889
Author(s):  
Nur Alia Johari ◽  
Kenny Voon ◽  
Shen Yung Toh ◽  
Lokman Hakim Sulaiman ◽  
Ivan Kok Seng Yap ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Virus Type ◽  
Aedes Albopictus ◽  
Peninsular Malaysia ◽  
Urban Setting

scholarly journals RNA Isolation of Dengue Virus Type 2 with Different Precipitation Solvents : Methanol, Chloroform, and 2-Isopropanol.

2018 ◽  
Vol 3 (1) ◽  
pp. 52
Author(s):  
Yovilianda Maulitiva Untoro ◽  
Teguh Hari Sucipto ◽  
Harsasi Setyawati ◽  
Siti Churrotin ◽  
Ilham Harlan Amarullah ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Virus Type ◽  
Aedes Albopictus ◽  
Rna Isolation ◽  
Guanidinium Thiocyanate ◽  
The World ◽  
Dengue Virus Type 2

Dengue virus distributed in tropical and subtropical regions in the world. DENV viruses are transmitted between humans primarily by Aedes aegypti and Aedes albopictus mosquitoes and are endemic in most areas in which the vectors occur. Four serotypes of dengue virus are DENV-1, DENV-2, DENV-3 and DENV-4. DENV-2 is comprised of six genotypes. Ethanol precipitation is a commonly used technique for concentrating and de-salting nucleic acids (DNA or RNA) preparations in aqueous solution. RNA isolation by combining Guanidinium thiocyanate and phenol reported has been reported. In this report, we investigated RNA isolation from DENV-2 using QIAamp Mini Kit with 2-Isopropanol, Methanol, Chloroform precipitation solvent. Electrophoregram showed DNA band as  the result of RNA isolation with methanol and 2-isopropanol are produced quite well. Dna band of the of RNA isolation with chloroform solvent has the lowest intensity than methanol and 2-isopropanol. This study showed that methanol and 2-isopropanol  can used as precipitation solvent for isolating RNA.

INFECTION AND DISSEMINATION OF DENGUE VIRUS TYPE 2 IN AEDES AEGYPTI, AEDES ALBOPICTUS, AND AEDES SCUTELLARIS FROM THE TORRES STRAIT, AUSTRALIA

2007 ◽  
Vol 23 (4) ◽  
pp. 383-388 ◽  
Author(s):  
PETER R. MOORE ◽  
PETRINA H. JOHNSON ◽  
GREG A. SMITH ◽  
SCOTT A. RITCHIE ◽  
ANDREW F. VAN DEN HURK
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Virus Type ◽  
Aedes Albopictus ◽  
Torres Strait ◽  
Dengue Virus Type 2

scholarly journals Dengue Virus Type 2 Infections of Aedes aegypti Are Modulated by the Mosquito's RNA Interference Pathway

2009 ◽  
Vol 5 (2) ◽  
pp. e1000299 ◽  
Author(s):  
Irma Sánchez-Vargas ◽  
Jaclyn C. Scott ◽  
B. Katherine Poole-Smith ◽  
Alexander W. E. Franz ◽  
Valérie Barbosa-Solomieu ◽  
...  
Keyword(s):  
Rna Interference ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Virus Type ◽  
Dengue Virus Type 2

Variation Among Strains of Aedes aegypti in Susceptibility to Oral Infection with Dengue Virus Type 2

1990 ◽  
Vol 43 (3) ◽  
pp. 308-313 ◽  
Author(s):  
Isabelle Tardieux ◽  
Laurent Lapchin ◽  
François Rodhain ◽  
Olivier Poupel
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Virus Type ◽  
Oral Infection ◽  
Dengue Virus Type 2

scholarly journals PERAN GEN STRUKTURAL, GEN NON-STRUKTURAL, DAN UNTRANSLATED REGION DALAM PERAKITAN VIRUS DENGUE

2015 ◽  
Vol 7 (2) ◽  
Author(s):  
Meiranty C. Pangerapan ◽  
Beivy J. Kolondam
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Dengue Fever ◽  
Aedes Albopictus ◽  
Untranslated Region ◽  
Rna Virus ◽  
Virus Genome ◽  
Interferon Response ◽  
Endoplasmic Reticulum Membrane ◽  
Structural Genes

Abstract: Dengue virus is a single-stranded RNA virus that belongs to Flaviviridae family. This virus causes dengue fever which is transmitted by Aedes aegypti dan Aedes albopictus. There are four serotypes of dengue virus; all of them can cause dengue fever. Understanding the genomics of dengue virus is important for research and diagnostics. The genome of dengue virus is 11 kilo-base long. It consists of 5’-untranslated region (5’-UTR), three structural genes (coding capsid protein, pre-membrane/membrane, and envelope), seven non-structural genes (coding NS1, NS2A, NS2B, NS3, NS4A, NS4B, and NS5 proteins) and 3’-UTR. Non-structural genes are encoding proteins of viral RNA replication, interferon response, viral assembly and secretion, endoplasmic reticulum membrane invagination induction, immune-mediator induction, and RNA 5’-caping.Keywords: dengue virus, genome, structural genes, non-structural genes, untranslated region.Abstrak: Virus dengue merupakan virus RNA beruntai tunggal yang termasuk dalam famili Flaviviridae. Virus ini adalah penyebab penyakit demam berdarah dengue yang ditransmisikan melalui nyamuk Aedes aegypti dan Aedes albopictus. Ada empat serotipe virus dengue yang telah dikenal secara luas yang ada semuanya dapat menimbulkan penyakit demam berdarah. Pemahaman tentang genomik virus dengue sangat penting untuk pengembangan penelitian dan juga untuk keperluan diagnostik. Genom virus dengue memiliki panjang 11 kilo basa. Genomnya tersusun atas 5’-untranslated region (5’-UTR), tiga gen struktural (mengodekan protein kapsid, premembran/membran dan amplop), tujuh gen non-struktural (mengodekan protein NS1, NS2A, NS2B, NS3, NS4A, NS4B dan NS5) dan 3’-UTR. Gen-gen non-struktural mengodekan protein untuk replikasi RNA virus, respon interferon, perakitan, sekresi partikel virus, menginduksi invaginasi membran retikulum endoplasma, induksi imunomediator dan penambahan tudung pada ujung 5’ RNA.Kata kunci: virus dengue, genom, gen struktural, gen non-struktural, untranslated region

scholarly journals Replication of Dengue Virus Type 2 in Aedes albopictus Cell Culture

1973 ◽  
Vol 12 (2) ◽  
pp. 275-283 ◽  
Author(s):  
Pantipa Sinarachatanant ◽  
Lloyd C. Olson
Keyword(s):  
Cell Culture ◽  
Dengue Virus ◽  
Virus Type ◽  
Aedes Albopictus ◽  
Albopictus Cell ◽  
Dengue Virus Type 2
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