scholarly journals Characterization of Trophoblast and Extraembryonic Endoderm Cell Lineages Derived from Rat Preimplantation Embryos

PLoS ONE ◽  
2010 ◽  
Vol 5 (3) ◽  
pp. e9794 ◽  
Author(s):  
Ilya Chuykin ◽  
Irina Lapidus ◽  
Elena Popova ◽  
Larisa Vilianovich ◽  
Valentina Mosienko ◽  
...  
Keyword(s):  
Preimplantation Embryos ◽  
Cell Lineages ◽  
Extraembryonic Endoderm ◽  
Endoderm Cell

scholarly journals Identification and Characterization of Extracellular Vesicles and Its DNA Cargo Secreted During Murine Embryo Development

Genes ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 203 ◽  
Author(s):  
Blanca Simon ◽  
David Bolumar ◽  
Alicia Amadoz ◽  
Jorge Jimenez-Almazán ◽  
Diana Valbuena ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Preimplantation Embryos ◽  
Gene Enrichment ◽  
Transmission Electron ◽  
Genome Wide ◽  
Significant Gene ◽  
Identification And Characterization ◽  
Old Females ◽  
Total Concentrations

Extracellular vesicles (EVs) are known to transport DNA, but their implications in embryonic implantation are unknown. The aim of this study was to investigate EVs production and secretion by preimplantation embryos and assess their DNA cargo. Murine oocytes and embryos were obtained from six- to eight-week-old females, cultured until E4.5 and analyzed using transmission electron microscopy to examine EVs production. EVs were isolated from E4.5-day conditioned media and quantified by nanoparticle tracking analysis, characterized by immunogold, and their DNA cargo sequenced. Multivesicular bodies were observed in murine oocytes and preimplantation embryos together with the secretion of EVs to the blastocoel cavity and blastocyst spent medium. Embryo-derived EVs showed variable electron-densities and sizes (20–500 nm) and total concentrations of 1.74 × 107 ± 2.60 × 106 particles/mL. Embryo secreted EVs were positive for CD63 and ARF6. DNA cargo sequencing demonstrated no differences in DNA between apoptotic bodies or smaller EVs, although they showed significant gene enrichment compared to control medium. The analysis of sequences uniquely mapping the murine genome revealed that DNA contained in EVs showed higher representation of embryo genome than vesicle-free DNA. Murine blastocysts secrete EVs containing genome-wide sequences of DNA to the medium, reinforcing the relevance of studying these vesicles and their cargo in the preimplantation moment, where secreted DNA may help the assessment of the embryo previous to implantation.

Flow cytometry enumeration and characterization of stem cell lineages in the liver of patients with hepatic tumors

2007 ◽  
Vol 39 (3) ◽  
pp. A1
Author(s):  
L. Porretti ◽  
D. Prati ◽  
F. Colombo ◽  
R. Gramignoli ◽  
A. Cattaneo ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Stem Cell ◽  
Hepatic Tumors ◽  
Cell Lineages

scholarly journals Isolation and characterization of porcine visceral endoderm cell lines derived from in vivo 11-day blastocysts

2007 ◽  
Vol 43 (2) ◽  
pp. 72-86 ◽  
Author(s):  
Neil C. Talbot ◽  
Le Ann Blomberg ◽  
Ayesha Mahmood ◽  
Thomas J. Caperna ◽  
Wesley M. Garrett
Keyword(s):  
Cell Lines ◽  
Visceral Endoderm ◽  
Endoderm Cell ◽  
Isolation And Characterization

scholarly journals ISOLATION AND CHARACTERIZATION OF A BOVINE VISCERAL ENDODERM CELL LINE DERIVED FROM A PARTHENOGENETIC BLASTOCYST

2005 ◽  
Vol 41 (5) ◽  
pp. 130 ◽  
Author(s):  
NEIL C. TALBOT ◽  
THOMAS J. CAPERNA ◽  
ANNE M. POWELL ◽  
ALAN D. EALY ◽  
LE ANN BLOMBERG ◽  
...  
Keyword(s):  
Cell Line ◽  
Visceral Endoderm ◽  
Endoderm Cell ◽  
Isolation And Characterization

scholarly journals SOX17 Is Not Required for the Derivation and Maintenance of Mouse Extraembryonic Endoderm Stem Cell Lines

2022 ◽  
Author(s):  
Xudong Dong ◽  
Ailing Ding ◽  
Jiangwei Lin
Keyword(s):  
Stem Cell ◽  
Cell Lines ◽  
Embryonic Stem ◽  
Cell Lineage ◽  
Preimplantation Embryos ◽  
Primitive Endoderm ◽  
Extraembryonic Endoderm ◽  
Gene Expression Analyses ◽  
Stem Cell Lines

Extraembryonic endoderm stem (XEN) cell lines can be derived and maintained in vitro and reflect the primitive endoderm cell lineage. SOX17 is thought to be required for the derivation and maintenance of mouse XEN cell lines. Here we have re-evaluated this requirement for SOX17. We derived multiple SOX17-deficient XEN cell lines from preimplantation embryos of a SOX17-Cre knockout strain and chemically converted multiple SOX17-deficient embryonic stem cell lines into XEN cell lines by transient culturing with retinoic acid and Activin A. We confirmed the XEN profile of SOX17-deficient cell lines by immunofluorescence with various markers, by NanoString gene expression analyses, and by their contribution to the extraembryonic endoderm of chimeric embryos produced by injecting these cells into blastocysts. Thus, SOX17 is not required for the derivation and maintenance of XEN cell lines.

scholarly journals Principal Components by FTIR Spectroscopy as Innovative Characterization Technique during Differentiation of Pluripotent Stem Cells to Pancreatic Cells

2017 ◽  
Author(s):  
◽  
M. M. Mata-Miranda
Keyword(s):  
Stem Cells ◽  
Ftir Spectroscopy ◽  
Pluripotent Stem Cells ◽  
Principal Component ◽  
Cell Lineages ◽  
Non Invasive ◽  
Differentiated Cells ◽  
Pancreatic Cells ◽  
Second Derivatives

Two of the greatest challenges in Stem Cells (SCs) biology and regenerative medicine, are differentiation control of SCs and ensuring the purity of differentiated cells. In this sense, fast, efficient and accurate techniques for SCs characterization and their differentiation into different cell lineages are needed. The aim of this study was to analyse Pluripotent Stem Cells (PSCs) and Differentiated Pancreatic Cells (DPCs) by Fourier Transform Infrared (FTIR) spectroscopy and Principal Component Analysis (PCA). For this purpose, we differentiated PSCs toward DPCs, characterizing the differentiation process at different stages (0, 11, 17 and 21 days) through light microscopy and vibrational spectroscopy. FTIR spectra were analysed with the multivariate method of PCA, using the second derivatives in the protein, carbohydrate and ribose regions. The results indicate that the PCA allows to characterize and discriminate PSCs and DPCs at different stages of differentiation in the analysed spectral regions. From these results, we concluded that the PCA allows the chemically and structural characterization of PSCs and the different stages of their differentiation in a fast, accurate and non-invasive way.

scholarly journals IDENTIFICATION AND CHARACTERIZATION OF 22 GENES THAT AFFECT THE VULVAL CELL LINEAGES OF THE NEMATODE CAENORHABDITIS ELEGANS

Genetics ◽  
1985 ◽  
Vol 110 (1) ◽  
pp. 17-72
Author(s):  
Edwin L Ferguson ◽  
H Robert Horvitz
Keyword(s):  
Caenorhabditis Elegans ◽  
Gene Function ◽  
Ventral Side ◽  
Recessive Mutation ◽  
Partial Reduction ◽  
Nematode Caenorhabditis Elegans ◽  
Multiple Alleles ◽  
Cell Lineages ◽  
Identification And Characterization

ABSTRACT Ninety-five mutants of the nematode Caenorhabditis elegans altered in the cell lineages of the vulva have been isolated on the basis of their displaying one of two phenotypes, Vulvaless or Multivulva. In Vulvaless mutants, which define 12 genes, no vulva is present. In Multivulva mutants, which define ten genes, one or more supernumerary vulva-like protrusions are located along the ventral side of the animal. A single recessive mutation is responsible for the phenotypes of most, but not all, of these strains. Fifteen of these 22 genes are represented by multiple alleles. We have shown by a variety of genetic criteria that mutations that result in a Vulvaless or Multivulva phenotype in six of the 22 genes most likely eliminate gene function. In addition, Vulvaless or Multivulva mutations in seven of the other genes most likely result in a partial reduction of gene function; the absence of the activity of any of these genes probably results in lethality or sterility. Our results suggest that we may have identified most, or all, genes of these two classes.

scholarly journals Characterization of discrete equine intestinal epithelial cell lineages

2015 ◽  
Vol 76 (4) ◽  
pp. 358-366 ◽  
Author(s):  
Liara M. Gonzalez ◽  
Leslie A. Kinnin ◽  
Anthony T. Blikslager
Keyword(s):  
Epithelial Cell ◽  
Intestinal Epithelial Cell ◽  
Intestinal Epithelial ◽  
Cell Lineages
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