scholarly journals Functional Imaging of Rel Expression in Inflammatory Processes Using Bioluminescence Imaging System in Transgenic Mice

PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e57632 ◽  
Author(s):  
Xingyu Yang ◽  
Hua Jing ◽  
Kai Zhao ◽  
Ruilin Sun ◽  
Zhenze Liu ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Functional Imaging ◽  
Bioluminescence Imaging ◽  
Imaging System ◽  
Inflammatory Processes

scholarly journals Functional imaging of interleukin 1 beta expression in inflammatory process using bioluminescence imaging in transgenic mice

2008 ◽  
Vol 9 (1) ◽  
pp. 49 ◽  
Author(s):  
Limei Li ◽  
Zhaoliang Fei ◽  
Jianke Ren ◽  
Ruilin Sun ◽  
Zhihui Liu ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Functional Imaging ◽  
Inflammatory Process ◽  
Bioluminescence Imaging ◽  
Interleukin 1 ◽  
Interleukin 1 Beta

scholarly journals Retinal functional imager (RFI): Non-invasive functional imaging of the retina

2013 ◽  
Vol 5 (2) ◽  
pp. 250-257 ◽  
Author(s):  
Sunil Ganekal
Keyword(s):  
Functional Imaging ◽  
Structural Changes ◽  
Imaging System ◽  
Retinal Blood Flow ◽  
Capillary Perfusion ◽  
Functional Changes ◽  
Non Invasive ◽  
Retinal Disorders ◽  
Retinal Blood Flow Velocity ◽  
Perfusion Maps

Retinal functional imager (RFI) is a unique non-invasive functional imaging system with novel capabilities for visualizing the retina. The objective of this review was to show the utility of non-invasive functional imaging in various disorders. Electronic literature search was carried out using the websites www.pubmed.gov and www.google.com. The search words were retinal functional imager and non-invasive retinal imaging used in combination. The articles published or translated into English were studied. The RFI directly measures hemodynamic parameters such as retinal blood-flow velocity, oximetric state, metabolic responses to photic activation and generates capillary perfusion maps (CPM) that provides retinal vasculature detail similar to flourescein angiography. All of these parameters stand in a direct relationship to the function and therefore the health of the retina, and are known to be degraded in the course of retinal diseases. Detecting changes in retinal function aid early diagnosis and treatment as functional changes often precede structural changes in many retinal disorders. Nepal J Ophthalmol 2013; 5(10): 250-257 DOI: http://dx.doi.org/10.3126/nepjoph.v5i2.8738

scholarly journals A LabVIEW Platform for Preclinical Imaging Using Digital Subtraction Angiography and Micro-CT

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Cristian T. Badea ◽  
Laurence W. Hedlund ◽  
G. Allan Johnson
Keyword(s):  
Digital Subtraction Angiography ◽  
Functional Imaging ◽  
Imaging System ◽  
Micro Ct ◽  
Digital Subtraction ◽  
X Ray ◽  
Retrospective Gating ◽  
Dual Source ◽  
Labview Platform ◽  
And Control

CT and digital subtraction angiography (DSA) are ubiquitous in the clinic. Their preclinical equivalents are valuable imaging methods for studying disease models and treatment. We have developed a dual source/detector X-ray imaging system that we have used for both micro-CT and DSA studies in rodents. The control of such a complex imaging system requires substantial software development for which we use the graphical language LabVIEW (National Instruments, Austin, TX, USA). This paper focuses on a LabVIEW platform that we have developed to enable anatomical and functional imaging with micro-CT and DSA. Our LabVIEW applications integrate and control all the elements of our system including a dual source/detector X-ray system, a mechanical ventilator, a physiological monitor, and a power microinjector for the vascular delivery of X-ray contrast agents. Various applications allow cardiac- and respiratory-gated acquisitions for both DSA and micro-CT studies. Our results illustrate the application of DSA for cardiopulmonary studies and vascular imaging of the liver and coronary arteries. We also show how DSA can be used for functional imaging of the kidney. Finally, the power of 4D micro-CT imaging using both prospective and retrospective gating is shown for cardiac imaging.

Multi-Channel Brain Functional Imaging System Based on Lock-in Photon Counting

2019 ◽  
Vol 46 (1) ◽  
pp. 0107001
Author(s):  
丁雪梅 Ding Xuemei ◽  
王兵元 Wang Bingyuan ◽  
刘东远 Liu Dongyuan ◽  
张耀 Zhang Yao ◽  
潘甜甜 Pan Tiantian ◽  
...  
Keyword(s):  
Functional Imaging ◽  
Imaging System ◽  
Photon Counting ◽  
Lock In

106 ASSESSMENT OF ANTI-BACTERIAL EFFECTS OF PEGYLATED SILVER-COATED CARBON NANOTUBES ON CAUSATIVE BACTERIA OF BOVINE INFERTILITY USING BIOLUMINESCENCE IMAGING SYSTEM

2017 ◽  
Vol 29 (1) ◽  
pp. 161 ◽  
Author(s):  
S. Park ◽  
A. A. Chaudhari ◽  
S. Pillai ◽  
S. R. Singh ◽  
S. T. Willard ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Carbon Nanotubes ◽  
Optical Density ◽  
Bacterial Infections ◽  
Pathogenic Bacteria ◽  
Bioluminescence Imaging ◽  
Imaging System ◽  
Control Group ◽  
Silver Nanotubes ◽  
Dose Dependent

Pathogenic bacteria including Escherichia coli and Salmonella sp. are the major causative agents of endometritis and can cause infertility in livestock animals. Antibiotics are commonly used to terminate bacterial infections, but the development of bacterial antibiotic resistance is often encountered. Nanotechnology associated with silver nanoparticles has been highlighted as an alternative anti-bacterial agent, and pegylated silver-coated single-walled carbon nanotubes have high anti-bacterial effects and are non-toxic to human and murine cells in vitro. Here we verified whether a real-time bioluminescence monitoring system could be an alternative tool to assess anti-bacterial effects of nanotubes in a noninvasive approach. Escherichia coli and Salmonella sp. were transfected with plasmids containing constructs for luciferase enzyme (LuxCDABE) and substrate (luciferin) to create self-illuminating bioluminescent bacteria. Pathogens were grown in LB broth at 37°C, adjusted to 107 cfu mL−1, and placed in 96-well plates for treatments. Pegylated (pSWCNTs-Ag) and non-pegylated (SWCNTs-Ag) nanotubes were prepared and added to culture wells at various concentrations (31.25–125 µg mL−1). The control group corresponded to bacteria without nanotubes (0 µg mL−1). Anti-bacterial effects of nanotubes were determined every 10 min until 1 h, then every 30 min up to 6 h incubation through optical density (600 nm) measurements and bioluminescence imaging (BLI) and quantification using an IVIS system. Optical density and BLI data were compared at each time-point using 2-way ANOVA, with P < 0.05 set for significance. Bioluminescence signals emitted by both bacteria stains appeared within 10 min of incubation. Thereafter, control bacteria showed exponential growth that was detected as early as 25 min post-incubation. Bioluminescence imaging revealed dose-dependent anti-bacterial activities of both pSWCNTs-Ag and SWCNTs-Ag on each E. coli and Salmonella sp. (P < 0.05). Contrary to BLI, the OD values did not always reflect bacteria concentrations, and varied according to nanotube concentrations. No significant differences in anti-bacterial activities were revealed between pSWCNTs-Ag and SWCNTs-Ag based on OD values during 6 h of incubation (P > 0.05); meanwhile, pSWCNTs-Ag nanotubes exhibited stronger anti-bacterial effects than SWCNTs-Ag during the same period using BLI (P < 0.05). In summary, we confirmed previous reports showing dose-dependent eliminations of pathogenic bacteria by silver nanotubes. Pegylated nanotubes exhibited high anti-bacterial activity compared to non-pegylated nanotubes. Bioluminescence imaging system revealed superior resolution to enable precise investigation of anti-bacterial kinetics of silver nanotubes. This feature could be useful for the study of bacterial infections that impair livestock fertility. Work was supported by USDA-ARS Biophotonics Initiative grant #58-6402-3-018.

scholarly journals Functional imaging diagnostic decentralization idea

2020 ◽  
Author(s):  
Sirocchi Luca ◽  
Sirocchi Andrea ◽  
Perrone Luca ◽  
Di Prete Giovanni
Keyword(s):  
Health Care ◽  
Functional Imaging ◽  
Care Provider ◽  
Imaging System ◽  
Private Health Care ◽  
Critical Importance ◽  
Locomotor System ◽  
Private Care ◽  
Surgery Outcome ◽  
Spine Disorders

Home imaging diagnostic means: a service to satisfy the needs of all those who, for many reasons, have to do radiographs and ultrasounds at home or in private care istitutions which got no imaging system. Examples: patients with severe diseases(accidents, after surgery outcome), cronich disease or locomotor system disorders (ex. spine disorders), affected by cardiorespiratory diseases or,also, the ones who cannot move. So,in the next future, we can see the healtcare facility critical importance for this service, it must be programmed. Now, to activate home diagnotic imaging (hospital or private health care provider), all the criteria is analysed.

Sign in / Sign up

Export Citation Format

Share Document