scholarly journals Quantitative DNA Methylation Analysis of Candidate Genes in Cervical Cancer

PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0122495 ◽  
Author(s):  
Erin M. Siegel ◽  
Bridget M. Riggs ◽  
Amber L. Delmas ◽  
Abby Koch ◽  
Ardeshir Hakam ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Dna Methylation ◽  
Candidate Genes ◽  
Methylation Analysis ◽  
Dna Methylation Analysis

scholarly journals Cervical cancer detection by DNA methylation analysis in urine

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Barbara C. Snoek ◽  
Annina P. van Splunter ◽  
Maaike C. G. Bleeker ◽  
Maartje C. van Ruiten ◽  
Daniëlle A. M. Heideman ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Dna Methylation ◽  
Cancer Detection ◽  
Methylation Analysis ◽  
Dna Methylation Analysis

scholarly journals CADM1, MAL and miR124-2 methylation analysis in cervical scrapes to detect cervical and endometrial cancer

2014 ◽  
Vol 67 (12) ◽  
pp. 1067-1071 ◽  
Author(s):  
Lise M A De Strooper ◽  
Marjolein van Zummeren ◽  
Renske D M Steenbergen ◽  
Maaike C G Bleeker ◽  
Albertus T Hesselink ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Dna Methylation ◽  
Endometrial Cancer ◽  
Gene Promoter ◽  
Intraepithelial Neoplasia ◽  
Promoter Hypermethylation ◽  
Methylation Analysis ◽  
Cervical Intraepithelial Neoplasia Grade ◽  
Specific Pcr ◽  
Dna Methylation Analysis

AimsGene promoter hypermethylation is recognised as an essential early step in carcinogenesis, indicating important application areas for DNA methylation analysis in early cancer detection. The current study was set out to assess the performance of CADM1, MAL and miR124-2 methylation analysis in cervical scrapes for detection of cervical and endometrial cancer.MethodsA series of cervical scrapes of women with cervical (n=79) or endometrial (n=21) cancer, cervical intraepithelial neoplasia grade 3 (CIN3) (n=16) or CIN2 (n=32), and women without evidence of CIN2 or worse (n=120) were assessed for methylation of CADM1, MAL and miR124-2. Methylation analysis was done by the PreCursor-M assay, a multiplex quantitative methylation-specific PCR.ResultsAll samples of women with cervical cancer (79/79, 100%), independent of the histotype, and 76% (16/21; 95% CI 58.0% to 94.4%) of women with endometrial cancer scored positive for DNA methylation for at least one of the three genes. In women without cancer, methylation frequencies increased significantly with severity of disease from 19.2% (23/120; 95% CI 12.1% to 26.2%) in women without CIN2 or worse to 37.5% (12/32; 95% CI 20.7% to 54.3%) and 68.8% (11/16; 95% CI 46.0% to 91.5%) in women with CIN2 and CIN3, respectively. Overall methylation positivity and the number of methylated genes increased proportionally to the lesion severity.ConclusionsDNA methylation analysis of CADM1, MAL and miR124-2 in cervical scrapes consistently detects cervical cancer and the majority of CIN3 lesions, and has the capacity to broaden its use on cervical scrapes through the detection of a substantial subset of endometrial carcinomas.

Quantitative promoter DNA methylation analysis of four candidate genes in anorexia nervosa: A pilot study

2013 ◽  
Vol 47 (2) ◽  
pp. 280-282 ◽  
Author(s):  
Eneda Pjetri ◽  
Emma Dempster ◽  
David A. Collier ◽  
Janet Treasure ◽  
Martien J. Kas ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Anorexia Nervosa ◽  
Pilot Study ◽  
Candidate Genes ◽  
Methylation Analysis ◽  
Promoter Dna Methylation ◽  
Promoter Dna ◽  
Dna Methylation Analysis

scholarly journals Targeted bisulfite sequencing: A novel tool for the assessment of DNA methylation with high sensitivity and increased coverage

2020 ◽  
Author(s):  
D.A. Moser ◽  
S. Müller ◽  
E.M. Hummel ◽  
A.S. Limberg ◽  
L. Dieckmann ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Candidate Genes ◽  
Regulatory Region ◽  
High Sensitivity ◽  
Healthy Controls ◽  
Methylation Analysis ◽  
Assay Sensitivity ◽  
Enhancer Element ◽  
Cost Efficient ◽  
Dna Methylation Analysis

AbstractDNA methylation analysis is increasingly used in stress research. Available methods are expensive, laborious and often limited by either the analysis of short CpG stretches or low assay sensitivity. Here, we present a cost-efficient next generation sequencing-based strategy for the simultaneous investigation of multiple candidate genes in large cohorts. To illustrate the method, we present analysis of four candidate genes commonly assessed in psychoneuroendocrine research: Glucocorticoid receptor (NR3C1), Serotonin transporter (SLC6A4), FKBP Prolyl isomerase 5 (FKBP5), and the Oxytocin receptor (OXTR).DNA methylation standards and DNA of a female and male donor were bisulfite treated in three independent trials and were used to generate sequencing libraries for 42 CpGs from the NR3C1 1F promoter region, 83 CpGs of the SLC6A4 5’ regulatory region, 5 CpGs located in FKBP5 intron 7, and additional 12 CpGs located in a potential enhancer element in intron 3 of the OXTR. In addition, DNA of 45 patients with borderline personality disorder (BPD) and 45 healthy controls was assayed. Multiplex libraries of all samples were sequenced on a MiSeq system and analyzed for mean methylation values of all CpG sites using amplikyzer2 software.Results indicated excellent accuracy of the assays when investigating replicates generated from the same bisulfite converted DNA, and very high linearity (R2> 0.9) of the assays shown by the analysis of differentially methylated DNA standards. Comparing DNA methylation between BPD and healthy controls revealed no biologically relevant differences.The technical approach as described here facilitates targeted DNA methylation analysis and represents a highly sensitive, cost-efficient and high throughput tool to close the gap between coverage and precision in epigenetic research of stress-associated phenotypes.

scholarly journals DNA methylation analysis of candidate genes associated with dementia in peripheral blood

Epigenomics ◽  
2020 ◽  
Vol 12 (23) ◽  
pp. 2109-2123
Author(s):  
Peter D Fransquet ◽  
Paul Lacaze ◽  
Richard Saffery ◽  
James Phung ◽  
Emily Parker ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Candidate Genes ◽  
Effect Size ◽  
Peripheral Blood ◽  
Healthy Individuals ◽  
Methylation Analysis ◽  
Blood Samples ◽  
Gender Education ◽  
Dna Methylation Analysis

Aim: To investigate whether genes implicated in dementia pathogenesis are differently methylated in peripheral blood. Materials & methods: Participants included 160 cognitively healthy individuals aged 70+ years: 73 who were subsequently diagnosed with dementia and 87 controls matched on age, gender, education, smoking and baseline cognition. A total of 49 participants also provided blood samples at diagnosis. Blood DNA methylation of APOE, APP, BDNF, PIN1, SNCA and TOMM40 was examined. Results: A total of 56 of 299 probes were differentially methylated in dementia compared with controls and 39 probes prior to diagnosis. The greatest effect size was in APP (cg19423170, Δ-8.32%, adjusted p = 0.009 at diagnosis; cg19933173, Δ-4.18%, adjusted p < 0.0001 prediagnosis). Conclusion: Genes implicated in dementia pathogenesis show differential blood methylation in dementia, even prior to diagnosis.

scholarly journals DNA methylation analysis in liquid-based cytology for cervical cancer screening

2009 ◽  
Vol 125 (12) ◽  
pp. 2995-3002 ◽  
Author(s):  
Sophia Apostolidou ◽  
Richard Hadwin ◽  
Matthew Burnell ◽  
Allison Jones ◽  
Donna Baff ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Dna Methylation ◽  
Cancer Screening ◽  
Cervical Cancer Screening ◽  
Methylation Analysis ◽  
Liquid Based Cytology ◽  
Dna Methylation Analysis
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