Transcript Dynamics at Early Stages of Molecular Interactions of MYMIV with Resistant and Susceptible Genotypes of the Leguminous Host, Vigna mungo

A total of 28 species of agromyzid flies belonging to 7 genera have been reared and described on 34 different leguminous host -plants from Pantnagar, Nainital, Northern India. 13 agromyzid flies have been described as new species viz., Japanagromyza species nova (sp.n.) ex leaves of Pueraria phaseoloides (Roxb.) Benth; Liriomyza sp.n. ex leaves of Vicia sativa Linn.; Liriomyza sp.n. ex leaves of Vicia faba Linn.; Malanagromyza sp.n. ex stems of Cassia sp.; Melanagromyza sp.n. ex stems of Pisum sativum Linn. Melanagromyza sp.n. ex stems of Trifolium pratense Linn.; Melanagromyza sp.n. ex stems of Vicia faba Linn.; Ophiomyia sp.n. ex stems of Pueraria Phaseoloides (Roxb.) Benth; Ophiomyia sp.n. ex stems of Sesbania aculeata Pers.; Ophiomyiz sp.n. ex stem of Vigna mungo (Linn.) Heeper and Ophiomyia sp.n. ex stems of Vigna mungo (Linn.) Heeper. Large numbers of host plants have been recorded as new host for various agromyzid species. More than one thousand male genitalia slides have been prepared for this study. Variations in morphology and genitalia characters have also been illustrated within the species. DOI: http://dx.doi.org/10.3126/njbs.v1i0.7474 Nepalese Journal of Biosciences 1: 66-82 (2011)

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Spherulitic crystal growth in the prodissoconch larval of Crassostrea virginica

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100076263 ◽

1983 ◽

Vol 41 ◽

pp. 518-519

Author(s):

George G. Cocks ◽

Louis Leibovitz ◽

DoSuk D. Lee

Keyword(s):

Crystal Structure ◽

Crassostrea Virginica ◽

Crystal Orientation ◽

Developmental Changes ◽

Gastrula Stage ◽

Orthorhombic Crystal ◽

Orthorhombic Crystal Structure ◽

Stages Of Growth ◽

Early Stages ◽

Initial Deposition

Our understanding of the structure and the formation of inorganic minerals in the bivalve shells has been considerably advanced by the use of electron microscope. However, very little is known about the ultrastructure of valves in the larval stage of the oysters. The present study examines the developmental changes which occur between the time of conception to the early stages of Dissoconch in the Crassostrea virginica(Gmelin), focusing on the initial deposition of inorganic crystals by the oysters.The spawning was induced by elevating the temperature of the seawater where the adult oysters were conditioned. The eggs and sperm were collected separately, then immediately mixed for the fertilizations to occur. Fertilized animals were kept in the incubator where various stages of development were stopped and observed. The detailed analysis of the early stages of growth showed that CaCO3 crystals(aragonite), with orthorhombic crystal structure, are deposited as early as gastrula stage(Figuresla-b). The next stage in development, the prodissoconch, revealed that the crystal orientation is in the form of spherulites.

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Formation of Dislocation Channels in Neutron Irradiated Molybdenum

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100096618 ◽

1972 ◽

Vol 30 ◽

pp. 672-673

Author(s):

S. Mahajan

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Ambient Temperature ◽

Room Temperature ◽

Channel Formation ◽

Early Stages ◽

Transmission Electron ◽

Three Stages ◽

Two Stages ◽

Polycrystalline Molybdenum

The evolution of dislocation channels in irradiated metals during deformation can be envisaged to occur in three stages: (i) formation of embryonic cluster free regions, (ii) growth of these regions into microscopically observable channels and (iii) termination of their growth due to the accumulation of dislocation damage. The first two stages are particularly intriguing, and we have attempted to follow the early stages of channel formation in polycrystalline molybdenum, irradiated to 5×1019 n. cm−2 (E > 1 Mev) at the reactor ambient temperature (∼ 60°C), using transmission electron microscopy. The irradiated samples were strained, at room temperature, up to the macroscopic yield point.Figure 1 illustrates the early stages of channel formation. The observations suggest that the cluster free regions, such as A, B and C, form in isolated packets, which could subsequently link-up to evolve a channel.

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Rotary Beveling for In Situ Thin-Section Microscopy of Cell Cultures

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100099192 ◽

1981 ◽

Vol 39 ◽

pp. 550-551

Author(s):

Dean A. Handley ◽

Jack T. Alexander ◽

Shu Chien

Keyword(s):

Cell Growth ◽

Cell Cultures ◽

Molecular Interactions ◽

Convenient Method ◽

Petri Dish ◽

Simple Method ◽

Thin Section Microscopy ◽

Thin Sectioning ◽

Organic Fluids

In situ preparation of cell cultures for ultrastructural investigations is a convenient method by which fixation, dehydration and embedment are carried out in the culture petri dish. The in situ method offers the advantage of preserving the native orientation of cell-cell interactions, junctional regions and overlapping configurations. In order to section after embedment, the petri dish is usually separated from the polymerized resin by either differential cryo-contraction or solvation in organic fluids. The remaining resin block must be re-embedded before sectioning. Although removal of the petri dish may not disrupt the native cellular geometry, it does sacrifice what is now recognized as an important characteristic of cell growth: cell-substratum molecular interactions. To preserve the topographic cell-substratum relationship, we developed a simple method of tapered rotary beveling to reduce the petri dish thickness to a dimension suitable for direct thin sectioning.

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