scholarly journals Elimination of Porcine Epidemic Diarrhea Virus in an Animal Feed Manufacturing Facility

PLoS ONE ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. e0169612 ◽  
Author(s):  
Anne R. Huss ◽  
Loni L. Schumacher ◽  
Roger A. Cochrane ◽  
Elizabeth Poulsen ◽  
Jianfa Bai ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Animal Feed ◽  
Manufacturing Facility ◽  
Diarrhea Virus ◽  
Feed Manufacturing ◽  
Porcine Epidemic Diarrhea

Prevalence and factors associated with the contamination of porcine epidemic diarrhea virus during pig transport to slaughterhouses in Colombia

2019 ◽  
Author(s):  
Maria del Pilar Pineda Ortiz ◽  
Johanna Paola Corrales Morales ◽  
Gilma Hernández Herrera ◽  
Carlos Enrique Trillos Pena ◽  
Diana Corina Zambrano Moreno
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Animal Feed ◽  
Epidemiological Data ◽  
Cross Sectional Study ◽  
Morbidity Rate ◽  
Cross Sectional ◽  
Diarrhea Virus ◽  
Factors Associated ◽  
Proportional Allocation ◽  
Porcine Epidemic Diarrhea

Abstract Abstract: Background: Porcine epidemic diarrhea (PED) is an infectious disease that mainly affects neonatal piglets with a morbidity rate of 80%–100% and a mortality rate of 50%–90%. Prior to March 2014, PED was an exotic disease in Colombia with no historical epidemiological data and this study was conducted at the beginning of the spread of the virus in the country. The aim of the present study was to determine the prevalence and factors associated with porcine epidemic diarrhea virus (PEDV) contamination in pig transportation to slaughterhouses in Colombia through a cross-sectional study involving a sample of 518 pig trucks visiting the 32 main slaughterhouses authorized by the regulatory agency of the govern, the INVIMA. Samples were obtained using proportional allocation stratified sampling of pig trucks entering and leaving slaughterhouses for the diagnosis of PEDV through RT-PCR, as well as conducting a survey of the drivers of the sampled trucks. The sampling was performed from June to November 2014, during the first outbreak. Results: The prevalence of PEDV at the time of entry of the plant was 71.8% (CI 95%: 70.8–72.8) versus 70.5% (CI 95%: 69.5–71.5) on exit (p=0.375). Associated factors found to increase the possibility of contamination included: the type of slaughterhouse either national (OR 15.9, CI 95%: 4.9–51.85) or national–exportation (OR 9.0, CI 95%: 2.20–36.91), the zone of highest slaughter (OR 9.05, CI 95%: 2.9–27.63), the non-exclusive use of vehicles for the transport of pigs (OR 3.75, CI 95%: 1.55–9.08) and visiting animal feed plants (OR 13.5, CI 95%: 4.1–44.12). Factors identified to reduce the possibility of contamination included: the cleaning of vehicles, the use of disinfectants on the vehicle and the exclusive of the vehicle for pig transportation. Conclusions: The results showed that the high degree of truck mobilization, with poor biosafety compliance and dissemination characteristics of the virus, facilitated the spread of PEDV throughout the national territory. These factors contributed to establishing the disease as an endemic problem in Colombia. Keywords: coronavirus, epidemiology, PEDV, prevalence, swine.

scholarly journals Characterizing the rapid spread of porcine epidemic diarrhea virus (PEDV) through an animal food manufacturing facility

PLoS ONE ◽  
2017 ◽  
Vol 12 (11) ◽  
pp. e0187309 ◽  
Author(s):  
Loni L. Schumacher ◽  
Anne R. Huss ◽  
Roger A. Cochrane ◽  
Charles R. Stark ◽  
Jason C. Woodworth ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Food Manufacturing ◽  
Animal Food ◽  
Manufacturing Facility ◽  
Diarrhea Virus ◽  
Rapid Spread ◽  
Porcine Epidemic Diarrhea

scholarly journals 163 Utilizing feed sequencing to decrease the risk of porcine epidemic diarrhea virus (PEDV) cross-contamination during feed manufacturing

2016 ◽  
Vol 94 (suppl_2) ◽  
pp. 76-76 ◽  
Author(s):  
L. L. Schumacher ◽  
R. A. Cochrane ◽  
J. C. Woodworth ◽  
A. R. Huss ◽  
C. R. Stark ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Cross Contamination ◽  
Diarrhea Virus ◽  
Feed Manufacturing ◽  
Porcine Epidemic Diarrhea

scholarly journals Prevalence and factors associated to contamination from porcine epidemic diarrhea virus during the transportation of pigs to slaughterhouses in Colombia

2020 ◽  
Author(s):  
Maria del Pilar Pineda Ortiz ◽  
Johanna Paola Corrales Morales ◽  
Gilma Hernández Herrera ◽  
Carlos Enrique Trillos Pena ◽  
Diana Corina Zambrano Moreno
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Animal Feed ◽  
The United States ◽  
The Body ◽  
Swine Industry ◽  
Pressurized Water ◽  
Diarrhea Virus ◽  
Factors Associated ◽  
Porcine Epidemic Diarrhea ◽  
The Impact

Abstract Porcine epidemic diarrhea virus (PEDV) causes acute diarrhea, dehydration, and high mortality in newborn piglets and has caused high economic impact in the swine industry in the United States and Asia. Until March 2014, PED was an exotic disease in Colombia. This study was carried out at the beginning of the spread of PEDV in the country, and its main objective was to determine the prevalence and factors associated to the contamination of PEDV in the transportation of pigs to the slaughterhouses in Colombia through environmental samples analyzed by RT-PCR. 518 pig trucks in the 32 main slaughterhouses, were sampled and the drivers of the trucks fulfilled a questionnaire. The prevalence of PEDV at the entrance of the slaughterhouses was 71.8% (CI 95%: 70.8-72.8) versus 70.5% (CI 95%: 69.5-71.5) at the slaughterhouse exit, and there was no evidence of significant differences between both rates. (McNemar value p: 0.375). The factors that increased the possibility of truck contamination were: vehicles that visit national slaughterhouses (OR 15.9 95% CI: 4.9–51.85) and that visit national –export type (OR 9.0 95% CI: 2.20–36.91), trucks with mobility in area of ​​greatest slaughter (OR 9.05 95% CI: 2.9-27.63), the non-exclusive use of vehicles to transport pigs (OR 3.75 95% CI: 1.55-9.08) and visit animal feed mills (OR 13.5 95% CI: 4.1– 44.12). The factors identified that reduce the possibility of contamination were cleanliness of the body truck (OR 0.089 95% CI: 0.03-0.23) and the cabin (OR 0.16 95%CI: 0.08-0.31), use of disinfectant (OR 0.32 95% CI:0.16-0.62), pressurized water (OR 0.38 95% CI:0.15-0.95), and back pump (OR 0.17 95% CI:0.08-0.35) and the exclusive use of the vehicle for pig transportation (OR 0.36 95% CI: 0.19-0.70). The results showed that the high grade of mobilization of the trucks to points of concentration, failures in biosecurity and virus survival characteristics, enabled the spread of PEDV, turning it into an endemic disease in Colombia; however, the measures implemented by the farmers mitigated the impact of the disease. We evidenced the need of standardizing and regulating biosecurity protocols for slaughterhouses, pig transportation and farms.

scholarly journals Using environmental sampling to evaluate the effectiveness of decontamination methods to reduce detection of porcine epidemic diarrhea virus RNA on feed manufacturing surfaces

2021 ◽  
Author(s):  
Mary B Muckey ◽  
Cassandra K Jones ◽  
Jason C Woodworth ◽  
Chad B Paulk ◽  
Steve S Dritz ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Sodium Hypochlorite ◽  
Porcine Epidemic Diarrhea Virus ◽  
Diarrhea Virus ◽  
Feed Manufacturing ◽  
Ct Value ◽  
Reverse Transcription Pcr ◽  
Virus Rna ◽  
Biological Hazard ◽  
Porcine Epidemic Diarrhea

Abstract Porcine epidemic diarrhea virus (PEDV) is a possible biological hazard in feed mills. If the virus enters a feed mill, it becomes widely distributed and is difficult to decontaminate from both feed contact and non-feed contact surfaces. The objective of this study was to evaluate a variety of liquid and dry decontamination treatments that could be used to reduce the amount of PEDV found on feed manufacturing surfaces. This experiment was designed as a 5 × 10 factorial with 5 different feed manufacturing surfaces and 10 decontamination treatments with 3 replicates of each combination. Surfaces included stainless steel, solid polyethylene, woven polypropylene tote bag, rubber, and sealed concrete coupons. One mL (1×10 5 TCID50/mL) of stock PEDV was applied to each surface and allowed to dry completely for 60 min. Next, for decontamination requiring surface application, the application was performed and allowed 15 min contact time. The quantity of PEDV RNA was determined using quantitative reverse transcription PCR. A decontamination treatment × surface interaction was observed (P < 0.0001), indicating the efficacy of treatment is dependent upon the surface in which it is applied. Within the cement surfaces, the sodium hypochlorite resulted in the greatest (P < 0.05) cycle threshold (Ct) value, followed by formaldehyde which had a greater (P < 0.05) Ct value compared to remaining treatments. Within polyethylene, rubber, and stainless steel surfaces, the formaldehyde treated surfaces had the greatest Ct values (P < 0.05), followed by the sodium hypochlorite treatment, with other treatments all having lower Ct values (P < 0.05). For the woven polyethylene surfaces, the formaldehyde and sodium hypochlorite treatments had greater Ct values compared to all other treatments (P < 0.05). Additional research is necessary to identify the role of decontamination treatment on PEDV infectivity and develop methods for decontamination of feed manufacturing facilities.

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