scholarly journals The wheat WRKY transcription factors TaWRKY49 and TaWRKY62 confer differential high-temperature seedling-plant resistance to Puccinia striiformis f. sp. tritici

PLoS ONE ◽  
2017 ◽  
Vol 12 (7) ◽  
pp. e0181963 ◽  
Author(s):  
Junjuan Wang ◽  
Fei Tao ◽  
Wei Tian ◽  
Zhongfeng Guo ◽  
Xianming Chen ◽  
...  
Keyword(s):  
Transcription Factors ◽  
High Temperature ◽  
Plant Resistance ◽  
Puccinia Striiformis ◽  
Wrky Transcription Factors ◽  
Seedling Plant

scholarly journals TaRPM1 Positively Regulates Wheat High-Temperature Seedling-Plant Resistance to Puccinia striiformis f. sp. tritici

2020 ◽  
Vol 10 ◽  
Author(s):  
Jiahui Wang ◽  
Wei Tian ◽  
Fei Tao ◽  
Jingjing Wang ◽  
Hongsheng Shang ◽  
...  
Keyword(s):  
High Temperature ◽  
Plant Resistance ◽  
Puccinia Striiformis ◽  
Seedling Plant

The RLK protein TaCRK10 activates wheat high‐temperature seedling‐plant resistance to stripe rust through interacting with TaH2A.1

2021 ◽  
Author(s):  
Jiahui Wang ◽  
Jingjing Wang ◽  
Juan Li ◽  
Hongsheng Shang ◽  
Xianming Chen ◽  
...  
Keyword(s):  
High Temperature ◽  
Stripe Rust ◽  
Plant Resistance ◽  
Seedling Plant

scholarly journals WRKY Transcription Factors Shared by BTH-Induced Resistance and NPR1-Mediated Acquired Resistance Improve Broad-Spectrum Disease Resistance in Wheat

2020 ◽  
Vol 33 (3) ◽  
pp. 433-443 ◽  
Author(s):  
Huanpeng Li ◽  
Jiaojiao Wu ◽  
Xiaofeng Shang ◽  
Miaomiao Geng ◽  
Jing Gao ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Disease Resistance ◽  
Induced Resistance ◽  
Broad Spectrum ◽  
Puccinia Striiformis ◽  
Acquired Resistance ◽  
Wrky Transcription Factors ◽  
Pathogen Invasion ◽  
Pathogenesis Related ◽  
Transgenic Lines

In Arabidopsis, both pathogen invasion and benzothiadiazole (BTH) treatment activate the nonexpresser of pathogenesis-related genes 1 (NPR1)-mediated systemic acquired resistance, which provides broad-spectrum disease resistance to secondary pathogen infection. However, the BTH-induced resistance in Triticeae crops of wheat and barley seems to be accomplished through an NPR1-independent pathway. In the current investigation, we applied transcriptome analysis on barley transgenic lines overexpressing wheat wNPR1 (wNPR1-OE) and knocking down barley HvNPR1 (HvNPR1-Kd) to reveal the role of NPR1 during the BTH-induced resistance. Most of the previously designated barley chemical-induced (BCI) genes were upregulated in an NPR1-independent manner, whereas the expression levels of several pathogenesis-related (PR) genes were elevated upon BTH treatment only in wNPR1-OE. Two barley WRKY transcription factors, HvWRKY6 and HvWRKY70, were predicted and further validated as key regulators shared by the BTH-induced resistance and the NPR1-mediated acquired resistance. Wheat transgenic lines overexpressing HvWRKY6 and HvWRKY70 showed different degrees of enhanced resistance to Puccinia striiformis f. sp. tritici pathotype CYR32 and Blumeria graminis f. sp. tritici pathotype E20. In conclusion, the transcriptional changes of BTH-induced resistance in barley were initially profiled, and the identified key regulators would be valuable resources for the genetic improvement of broad-spectrum disease resistance in wheat. [Formula: see text] Copyright © 2020 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .

scholarly journals Revealing Differentially Expressed Genes and Identifying Effector Proteins of Puccinia striiformis f. sp. tritici in Response to High-Temperature Seedling Plant Resistance of Wheat Based on Transcriptome Sequencing

mSphere ◽  
2020 ◽  
Vol 5 (3) ◽  
Author(s):  
Fei Tao ◽  
Yangshan Hu ◽  
Chang Su ◽  
Juan Li ◽  
Lili Guo ◽  
...  
Keyword(s):  
High Temperature ◽  
Differentially Expressed Genes ◽  
Puccinia Striiformis ◽  
Effector Proteins ◽  
Differentially Expressed ◽  
Content Type ◽  
Molecular Pattern ◽  
Seedling Plant ◽  
Effector Triggered Immunity ◽  
Pathogen Associated Molecular Pattern

ABSTRACT Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most important diseases of wheat (Triticum aestivum L.) globally. Recently, more aggressive Pst races have evolved to acquire new virulence profiles and are adapted better to high temperature than most of the previous races. Breeding cultivars with durable high-temperature seedling-plant (HTSP) resistance is an important strategy for controlling stripe rust. Understanding the mechanism of wheat HTSP resistance against Pst is important for more efficient breeding to improve host resistance. In the present study, transcriptomic analysis identified 25 Pst differentially expressed genes (DEGs) that were involved in the HTSP resistance in wheat cultivar Xiaoyan6 (XY6). Functional annotation indicated that these DEGs are related to membrane proteins, mRNA binding proteins, cell membrane transporters, and synthesis of cell nitrogen compounds. Among these DEGs, a candidate effector, PstCEP1 (PSTG_13342), was identified and cloned, and its function was verified. Barley stripe mosaic virus (BSMV)-mediated host-induced gene silencing (HIGS) of PstCEP1 reduced Pst virulence. Signal peptide verification and functional testing in Nicotiana benthamiana indicated that PstCEP1 is a secreted protein and has the function of suppressing programmed cell death (PCD). PstCEP1 as a candidate effector was further supported by type three secretion system (TTSS)-mediated overexpression responding to wheat HTSP resistance via affecting the pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) and effector-triggered immunity (ETI). IMPORTANCE In the present study, we performed transcriptomic analysis to identify differentially expressed genes and effector proteins of Puccinia striiformis f. sp. tritici (Pst) in response to the high-temperature seedling-plant (HTSP) resistance in wheat. Experimental validation confirmed the function of the highest upregulated effector protein, PstCEP1. This study provides a key resource for understanding the biology and molecular basis of Pst responses to wheat HTSP resistance, and PstCEP1 may be used in future studies to understand pathogen-associated molecular pattern-triggered immunity and effector-triggered immunity processes in the Pst-wheat interaction system.

scholarly journals TaXa21, a Leucine-Rich Repeat Receptor–Like Kinase Gene Associated with TaWRKY76 and TaWRKY62, Plays Positive Roles in Wheat High-Temperature Seedling Plant Resistance to Puccinia striiformis f. sp. tritici

2019 ◽  
Vol 32 (11) ◽  
pp. 1526-1535 ◽  
Author(s):  
Jiahui Wang ◽  
Junjuan Wang ◽  
Hongsheng Shang ◽  
Xianming Chen ◽  
Xiangming Xu ◽  
...  
Keyword(s):  
High Temperature ◽  
Puccinia Striiformis ◽  
Kinase Domain ◽  
Signal Pathways ◽  
Leucine Rich Repeat ◽  
Virus Induced Gene Silencing ◽  
Kinase Gene ◽  
Rice Bacterial Blight ◽  
Receptor Like Kinase ◽  
Seedling Plant

Puccinia striiformis f. sp. tritici causes wheat stripe rust, one of most important diseases of wheat worldwide. High-temperature seedling plant (HTSP) resistance of wheat to P. striiformis f. sp. tritici is one specific type of host resistance, induced by high temperature (HT). Receptor-like kinases (RLKs) play key roles in regulating plant development and signaling networks, but there have been no reports on possible roles played by RLKs in wheat HTSP to P. striiformis f. sp. tritici. In the present study, a leucine rich repeat (LRR)-RLK gene, TaXa21, with a high homology with rice bacterial blight resistance gene Xa21, was cloned from wheat cultivar Xiaoyan 6 (XY 6). TaXa21 expression was up-regulated by the exposure to HT (20°C) for 24 h at 8 days postinoculation with P. striiformis f. sp. tritici and was induced by ethylene (ET) and hydrogen peroxide (H2O2). Knocking down TaXa21 using virus-induced gene silencing reduced HTSP resistance to P. striiformis f. sp. tritici compared with the control plants. In addition, the expression level of TaCAT in the H2O2 pathway was induced and TaACO in the ET signal pathway was reduced in the HT-treated TaXa21-silenced plants. Transient expression of TaXa21 in tobacco leaves confirmed its subcellular localization in plasma membrane, consistent with the prediction from bioinformatics analysis. The transmembrane and kinase domain of TaXa21 can interact with TaWRKY76 in the nucleus and cell membrane, which is different from the localization of Xa21 in rice. The interaction between TaWRKY76 and TaWRKY62 (positively involved in the HTSP resistance of XY 6) were observed. Together, these results indicated that TaXa21 is a RLK associated with TaWRKY76 and TaWRKY62 and functions as a positive regulator of wheat HTSP resistance to P. striiformis f. sp. tritici. Furthermore, the host defense is mediated by the H2O2 and ET signal pathways.

NBS-LRR gene TaRPS2 is positively associated with the high-temperature seedling plant resistance of wheat against Puccinia striiformis f. sp. tritici

2020 ◽  
Author(s):  
Yangshan Hu ◽  
Fei Tao ◽  
Chang Su ◽  
Yue Zhang ◽  
Juan Li ◽  
...  
Keyword(s):  
High Temperature ◽  
Plasma Membranes ◽  
Puccinia Striiformis ◽  
Triticum Aestivum L ◽  
Temperature Treatment ◽  
Expression Level ◽  
Virus Induced Gene Silencing ◽  
Seedling Plant ◽  
Ros Burst ◽  
Wheat Leaves

Xiaoyan6 (XY6) is a wheat (Triticum aestivum L.) cultivar possessing non-race-specific high-temperature seedling plant (HTSP) resistance against stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst). Previously, we identified one particular gene, TaRPS2, for its involvement in the HTSP resistance. To elucidate the role of TaRPS2 in the HTSP resistance, we cloned the full length of TaRPS2 from XY6. The transcriptional expression of TaRPS2 was rapidly up-regulated (19.11-fold) under the normal-high-normal temperature treatment that induces the HTSP resistance. The expression level of TaRPS2 in leaves was higher than that in the stems and roots. Quantification of the endogenous hormones in wheat leaves after Pst-inoculation showed that 1-aminocyclopropane-1-carboxylic acid (ACC), salicylic acid (SA), and jasmonic acid (JA) were involved in the HTSP resistance. In addition, detection of the H2O2 accumulation indicated that reactive oxygen species (ROS) burst was also associated with the HTSP resistance. Two hours after exogenous H2O2 treatment or 0.5 h after SA treatment, the expression level of TaRPS2 was increased by 2.66 times and 2.35 times, respectively. The subcellular localization of eGFP-TaRPS2 fusion protein was in the nucleus and plasma membranes. Virus-induced gene silencing of TaRPS2 reduced the level of HTSP resistance in XY6. Compared with the non-silenced leaves, the TaRPS2-silenced leaves had the reduction of necrotic cells but a greater number of uredinia. These results indicated that TaRPS2 positively regulates the HTSP resistance of XY6 against Pst and is related to the SA and H2O2 signaling pathways.

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