scholarly journals Discovery of microRNA-like RNAs during early fruiting body development in the model mushroom Coprinopsis cinerea

PLoS ONE ◽  
2018 ◽  
Vol 13 (9) ◽  
pp. e0198234 ◽  
Author(s):  
Amy Yuet Ting Lau ◽  
Xuanjin Cheng ◽  
Chi Keung Cheng ◽  
Wenyan Nong ◽  
Man Kit Cheung ◽  
...  
Keyword(s):  
Fruiting Body ◽  
Coprinopsis Cinerea ◽  
Body Development ◽  
Fruiting Body Development

scholarly journals Strand-Specific RNA-Seq Analyses of Fruiting Body Development in Coprinopsis cinerea

PLoS ONE ◽  
2015 ◽  
Vol 10 (10) ◽  
pp. e0141586 ◽  
Author(s):  
Hajime Muraguchi ◽  
Kiwamu Umezawa ◽  
Mai Niikura ◽  
Makoto Yoshida ◽  
Toshinori Kozaki ◽  
...  
Keyword(s):  
Fruiting Body ◽  
Rna Seq ◽  
Coprinopsis Cinerea ◽  
Body Development ◽  
Fruiting Body Development

scholarly journals 5'-Serial Analysis of Gene Expression studies reveal a transcriptomic switch during fruiting body development in Coprinopsis cinerea

BMC Genomics ◽  
2013 ◽  
Vol 14 (1) ◽  
pp. 195 ◽  
Author(s):  
Chi Keung Cheng ◽  
Chun Hang Au ◽  
Sarah K Wilke ◽  
Jason E Stajich ◽  
Miriam E Zolan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fruiting Body ◽  
Coprinopsis Cinerea ◽  
Body Development ◽  
Expression Studies ◽  
Fruiting Body Development ◽  
Gene Expression Studies

scholarly journals Discovery of microRNA-like RNAs during early fruiting body development in the model mushroom Coprinopsis cinerea

2018 ◽  
Author(s):  
Amy Yuet Ting Lau ◽  
Xuanjin Cheng ◽  
Chi Keung Cheng ◽  
Wenyan Nong ◽  
Man Kit Cheung ◽  
...  
Keyword(s):  
Fruiting Body ◽  
Developmental Stages ◽  
Expression Patterns ◽  
Regulation Of Gene Expression ◽  
Evolutionary Relationship ◽  
Mirna Biogenesis ◽  
Coprinopsis Cinerea ◽  
Stem Loop ◽  
Body Development ◽  
Fruiting Body Development

AbstractCoprinopsis cinerea is a model mushroom particularly suited to study fungal fruiting body development and the evolution of multicellularity in fungi. While microRNAs (miRNAs) are extensively studied in animals and plants for their essential roles in post-transcriptional regulation of gene expression, miRNAs in fungi are less well characterized and their potential roles in controlling mushroom development remain unknown. To identify miRNA-like RNAs (milRNAs) in C. cinerea and explore their expression patterns during the early developmental transition of mushroom development, small RNA libraries of vegetative mycelium and primordium were generated and putative milRNA candidates were identified following the standards of miRNA prediction in animals and plants. Two out of 22 novel predicted milRNAs, cci-milR-12c and cci-milR-13e-5p, were validated by northern blot and stem-loop reverse transcription real-time PCR. Cci-milR-12c was differentially expressed whereas the expression levels of cci-milR-13e-5p were similar in the two developmental stages. Target prediction of the validated milRNAs resulted in genes associated with fruiting body development, including pheromone, hydrophobin, cytochrome P450, and protein kinase. Besides, essential genes for miRNA biogenesis, including three coding for Dicer-like (DCL), two for Argonaute-like (AGO-like) and one for quelling deficient-2 (QDE-2) proteins, were identified in the C. cinerea genome. Phylogenetic analysis showed that the DCL and AGO-like proteins of C. cinerea were more closely related to those in other basidiomycetes and ascomycetes than to animals and plants. Taken together, our findings provided the first evidence of milRNAs in the model mushroom and their potential roles in regulating fruiting body development. Information on the evolutionary relationship of milRNA biogenesis proteins across kingdoms has also provided new insights into further functional and evolutionary studies of miRNAs.

scholarly journals Transcriptional profiling elucidates the essential role of glycogen synthase kinase 3 to fruiting body formation in Coprinopsis cinerea

2018 ◽  
Author(s):  
Kathy PoLam Chan ◽  
Jinhui Chang ◽  
Yichun Xie ◽  
Man Kit Cheung ◽  
Ka Lee Ma ◽  
...  
Keyword(s):  
Fruiting Body ◽  
Glycogen Synthase ◽  
Glycogen Synthase Kinase 3 ◽  
Coprinopsis Cinerea ◽  
Fruiting Body Formation ◽  
Body Formation ◽  
Body Development ◽  
Fruiting Body Development ◽  
Control Samples ◽  
Time Point

The functions of glycogen synthase kinase 3 (GSK3) have been well-studied in animal, plant and yeast. However, information on its roles in basidiomycetous fungi is still limited. In this study, we used the model mushroom Coprinopsis cinerea to study the characteristics of GSK3 in fruiting body development. Application of a GSK3 inhibitor Lithium chloride (LiCl) induced enhanced mycelial growth and inhibited fruiting body formation in C. cinerea. RNA-Seq of LiCl-treated C. cinerea resulted in a total of 14128 unigenes. There were 1210 differentially expressed genes (DEGs) between the LiCl-treated samples and control samples in the mycelium stage (first time point), whereas 1402 DEGs were detected at the stage when the control samples formed hyphal knots and the treatment samples were still in mycelium (second time point). Kyoto Encyclopedia of Genes and Genome (KEGG) pathway enrichment analysis of the DEGs revealed significant associations between the enhanced mycelium growth in LiCl treated C. cinerea and metabolism pathways such as “biosynthesis of secondary metabolite” and “biosynthesis of antibiotics”. In addition, DEGs involved in cellular process pathways, including “cell cycle-yeast” and “meiosis-yeast”, were identified in C. cinerea fruiting body formation suppressed by LiCl under favorable environmental conditions. Our findings suggest that GSK3 activity is essential for fruiting body formation as it affects the expression of fruiting body induction genes and genes in cellular processes. Further functional studies of GSK3 in basidiomycetous fungi may help understand the relationships between environmental signals and fruiting body development.

scholarly journals Characterization of Serine Proteinase Expression in Agaricus bisporus and Coprinopsis cinerea by Using Green Fluorescent Protein and the A. bisporus SPR1 Promoter

2008 ◽  
Vol 75 (3) ◽  
pp. 792-801 ◽  
Author(s):  
Mary N. Heneghan ◽  
Claudine Porta ◽  
Cunjin Zhang ◽  
Kerry S. Burton ◽  
Michael P. Challen ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fruiting Body ◽  
Agaricus Bisporus ◽  
Fluorescent Protein ◽  
Serine Proteinase ◽  
Developmental Expression ◽  
Coprinopsis Cinerea ◽  
Body Development ◽  
Fruiting Body Development ◽  
Green Fluorescent

ABSTRACT The Agaricus bisporus serine proteinase 1 (SPR1) appears to be significant in both mycelial nutrition and senescence of the fruiting body. We report on the construction of an SPR promoter::green fluorescent protein (GFP) fusion cassette, pGreen_hph1_SPR_GFP, for the investigation of temporal and developmental expression of SPR1 in homobasidiomycetes and to determine how expression is linked to physiological and environmental stimuli. Monitoring of A. bisporus pGreen_hph1_SPR_GFP transformants on media rich in ammonia or containing different nitrogen sources demonstrated that SPR1 is produced in response to available nitrogen. In A. bisporus fruiting bodies, GFP activity was localized to the stipe of postharvest senescing sporophores. pGreen_hph1_SPR_GFP was also transformed into the model basidiomycete Coprinopsis cinerea. Endogenous C. cinerea proteinase activity was profiled during liquid culture and fruiting body development. Maximum activity was observed in the mature cap, while activity dropped during autolysis. Analysis of the C. cinerea genome revealed seven genes showing significant homology to the A. bisporus SPR1 and SPR2 genes. These genes contain the aspartic acid, histidine, and serine residues common to serine proteinases. Analysis of the promoter regions revealed at least one CreA and several AreA regulatory motifs in all sequences. Fruiting was induced in C. cinerea dikaryons, and fluorescence was determined in different developmental stages. GFP expression was observed throughout the life cycle, demonstrating that serine proteinase can be active in all stages of C. cinerea fruiting body development. Serine proteinase expression (GFP fluorescence) was most concentrated during development of young tissue, which may be indicative of high protein turnover during cell differentiation.

scholarly journals Lessons on fruiting body morphogenesis from genomes and transcriptomes of Agaricomycetes

2021 ◽  
Author(s):  
Laszlo G Nagy ◽  
Peter Jan Vonk ◽  
Markus Kunzler ◽  
Csenge Foldi ◽  
Mate Viragh ◽  
...  
Keyword(s):  
Cell Wall ◽  
Fruiting Body ◽  
Expression Patterns ◽  
Schizophyllum Commune ◽  
Gene Families ◽  
Second Phase ◽  
Fruiting Bodies ◽  
Body Development ◽  
Fruiting Body Development

Fruiting bodies of mushroom-forming fungi (Agaricomycetes) are among the most complex structures produced by fungi. Unlike vegetative hyphae, fruiting bodies grow determinately and follow a genetically encoded developmental program that orchestrates tissue differentiation, growth and sexual sporulation. In spite of more than a century of research, our understanding of the molecular details of fruiting body morphogenesis is limited and a general synthesis on the genetics of this complex process is lacking. In this paper, we aim to comprehensively identify conserved genes related to fruiting body morphogenesis and distill novel functional hypotheses for functionally poorly characterized genes. As a result of this analysis, we report 921 conserved developmentally expressed gene families, only a few dozens of which have previously been reported in fruiting body development. Based on literature data, conserved expression patterns and functional annotations, we provide informed hypotheses on the potential role of these gene families in fruiting body development, yielding the most complete description of molecular processes in fruiting body morphogenesis to date. We discuss genes related to the initiation of fruiting, differentiation, growth, cell surface and cell wall, defense, transcriptional regulation as well as signal transduction. Based on these data we derive a general model of fruiting body development, which includes an early, proliferative phase that is mostly concerned with laying out the mushroom body plan (via cell division and differentiation), and a second phase of growth via cell expansion as well as meiotic events and sporulation. Altogether, our discussions cover 1480 genes of Coprinopsis cinerea, and their orthologs in Agaricus bisporus, Cyclocybe aegerita, Armillaria ostoyae, Auriculariopsis ampla, Laccaria bicolor, Lentinula edodes, Lentinus tigrinus, Mycena kentingensis, Phanerochaete chrysosporium, Pleurotus ostreatus, and Schizophyllum commune, providing functional hypotheses for ~10% of genes in the genomes of these species. Although experimental evidence for the role of these genes will need to be established in the future, our data provide a roadmap for guiding functional analyses of fruiting related genes in the Agaricomycetes. We anticipate that the gene compendium presented here, combined with developments in functional genomics approaches will contribute to uncovering the genetic bases of one of the most spectacular multicellular developmental processes in fungi. Key words: functional annotation; comparative genomics; cell wall remodeling; development; fruiting body morphogenesis; mushroom; transcriptome

Sign in / Sign up

Export Citation Format

Share Document