scholarly journals Low-intensity pulsed ultrasound reduces periodontal atrophy in occlusal hypofunctional teeth

2017 ◽  
Vol 87 (5) ◽  
pp. 709-716 ◽  
Author(s):  
Yuki Kasahara ◽  
Risa Usumi-Fujita ◽  
Jun Hosomichi ◽  
Sawa Kaneko ◽  
Yuji Ishida ◽  
...  
Keyword(s):  
Alveolar Bone ◽  
Bhlh Transcription Factor ◽  
Mrna Levels ◽  
Micro Ct ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Qrt Pcr ◽  
Low Intensity ◽  
Normal Occlusion ◽  
Ct Analysis

ABSTRACT Objective: To clarify whether low-intensity pulsed ultrasound (LIPUS) exposure has recovery effects on the hypofunctional periodontal ligament (PDL) and interradicular alveolar bone (IRAB). Materials and Methods: Twelve-week-old male Sprague-Dawley rats were divided into three groups (n = 5 each): a normal occlusion (C) group, an occlusal hypofunction (H) group, and an occlusal hypofunction group subjected to LIPUS (HL) treatment. Hypofunctional occlusion of the maxillary first molar (M1) of the H and HL groups was induced by the bite-raising technique. Only the HL group was irradiated with LIPUS for 5 days. The IRAB and PDL of M1 were examined by microcomputed tomography (micro-CT) analysis. To quantify mRNA expression of cytokines involved in PDL proliferation and development, real-time reverse transcription quantitative PCR (qRT-PCR) was performed for twist family bHLH transcription factor 1 (Twist1), periostin, and connective tissue growth factor (CTGF) in the PDL samples. Results: Micro-CT analysis showed that the PDL volume was decreased in the H group compared with that of the C and HL groups. Both bone volume per tissue volume (BV/TV) of IRAB was decreased in the H group compared with that in the C group. LIPUS exposure restored BV/TV in the IRAB of the HL group. qRT-PCR analysis showed that Twist1, periostin, and CTGF mRNA levels were decreased in the H group and increased in the HL group. Conclusion: LIPUS exposure reduced the atrophic changes of alveolar bone by inducing the upregulation of periostin and CTGF expression to promote PDL healing after induction of occlusal hypofunction.

Low-intensity pulsed ultrasound promotes alveolar bone regeneration in a periodontal injury model

Ultrasonics ◽  
2018 ◽  
Vol 90 ◽  
pp. 166-172 ◽  
Author(s):  
Yunji Wang ◽  
Ye Qiu ◽  
Jie Li ◽  
Chunliang Zhao ◽  
Jinlin Song
Keyword(s):  
Bone Regeneration ◽  
Alveolar Bone ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Injury Model

scholarly journals Low-intensity Pulsed Ultrasound regulates alveolar bone homeostasis in experimental Periodontitis by diminishing Oxidative Stress

Theranostics ◽  
2020 ◽  
Vol 10 (21) ◽  
pp. 9789-9807
Author(s):  
Siqi Ying ◽  
Minmin Tan ◽  
Ge Feng ◽  
Yunchun Kuang ◽  
Duanjing Chen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Alveolar Bone ◽  
Bone Homeostasis ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Experimental Periodontitis

scholarly journals Microbubble-Mediated Ultrasound Outweighs Low-Intensity Pulsed Ultrasound on Osteogenesis and Neovascularization in a Rabbit Model of Steroid-Associated Osteonecrosis

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Dan-Feng Xu ◽  
Guo-Xin Qu ◽  
Shi-Gui Yan ◽  
Xun-Zi Cai
Keyword(s):  
Animal Model ◽  
Femoral Head ◽  
Rabbit Model ◽  
Mineral Apposition Rate ◽  
Histological Evaluation ◽  
Pulsed Ultrasound ◽  
Mineral Density ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Ct Analysis

Microbubbles magnify the acoustic pressure of low-intensity pulsed ultrasound (LIPUS) and may enhance its bioeffect for diagnostic and therapeutic purposes. This study compared the effect of this novel microbubble-mediated ultrasound (MUS) with that of the traditional LIPUS on osteogenesis and neovascularization in a rabbit model of steroid-associated osteonecrosis. We hypothesized that MUS might outweigh LIPUS on promoting osteogenesis and neovascularization in steroid-associated osteonecrosis. The bilateral femoral head necrosis was induced by lipopolysaccharide and methylprednisolone in the rabbits. The indices of bone mineral density (BMD), trabecular number, maximal loading strength, and mineral apposition rate were analyzed, demonstrating that the animal model of steroid-associated osteonecrosis was successfully established. Both the MUS group (GM) and the LIPUS group (GL) were insonated 20 min daily for six weeks. GM received an extra intracapsular injection of microbubbles before insonation every other day. Fluorescence bone labeling, Micro-CT Analysis, biomechanical test, quantitative real-time PCR, Western blot analysis, and histological evaluation were performed for comparing GM with GL. The results demonstrated a 39% higher mineral apposition rate in GM compared with GL. The BMD and the maximal loading strength of femoral head of GM increased by 4.3% and 27.8% compared to those of GL, respectively. The mRNA and protein expression of BMP-2 and VEGF were also significantly higher in GM. The number of blood vessels of GM was 65% greater than that of GL. MUS is more potent than LIPUS in enhancing osteogenesis, neovascularization, and biomechanical strength of femoral head in the animal model of steroid-associated osteonecrosis. Without increasing the intensity of insonation or the risk of tissue damage, MUS is better for inhibiting the process of steroid-associated osteonecrosis.

scholarly journals In VitroEffects of Low-Intensity Pulsed Ultrasound Stimulation on the Osteogenic Differentiation of Human Alveolar Bone-Derived Mesenchymal Stem Cells for Tooth Tissue Engineering

2013 ◽  
Vol 2013 ◽  
pp. 1-15 ◽  
Author(s):  
KiTaek Lim ◽  
Jangho Kim ◽  
Hoon Seonwoo ◽  
Soo Hyun Park ◽  
Pill-Hoon Choung ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Viability ◽  
Osteogenic Differentiation ◽  
Alveolar Bone ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Ultrasound Stimulation ◽  
Duty Cycles

Ultrasound stimulation produces significant multifunctional effects that are directly relevant to alveolar bone formation, which is necessary for periodontal healing and regeneration. We focused to find out effects of specific duty cycles and the percentage of time that ultrasound is being generated over one on/off pulse period, under ultrasound stimulation. Low-intensity pulsed ultrasound ((LIPUS) 1 MHz) with duty cycles of 20% and 50% was used in this study, and human alveolar bone-derived mesenchymal stem cells (hABMSCs) were treated with an intensity of 50 mW/cm2and exposure time of 10 min/day. hABMSCs exposed at duty cycles of 20% and 50% had similar cell viability (O.D.), which was higher (*P<0.05) than that of control cells. The alkaline phosphatase (ALP) was significantly enhanced at 1 week with LIPUS treatment in osteogenic cultures as compared to control. Gene expressions showed significantly higher expression levels of CD29, CD44, COL1, and OCN in the hABMSCs under LIPUS treatment when compared to control after two weeks of treatment. The effects were partially controlled by LIPUS treatment, indicating that modulation of osteogenesis in hABMSCs was related to the specific stimulation. Furthermore, mineralized nodule formation was markedly increased after LIPUS treatment than that seen in untreated cells. Through simple staining methods such as Alizarin red and von Kossa staining, calcium deposits generated their highest levels at about 3 weeks. These results suggest that LIPUS could enhance the cell viability and osteogenic differentiation of hABMSCs, and could be part of effective treatment methods for clinical applications.

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