scholarly journals DArTSeq SNP-based markers revealed high genetic diversity and structured population in Ethiopian cowpea [Vigna unguiculata (L.) Walp] germplasms

PLoS ONE ◽  
2020 ◽  
Vol 15 (10) ◽  
pp. e0239122
Author(s):  
Selamawit Ketema ◽  
Bizuayehu Tesfaye ◽  
Gemechu Keneni ◽  
Berhanu Amsalu Fenta ◽  
Ermias Assefa ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Vigna Unguiculata ◽  
High Genetic Diversity ◽  
Structured Population

scholarly journals Sources of resistance to black aphid in cowpea varieties used as green grains

2020 ◽  
Vol 13 ◽  
pp. e902
Author(s):  
Vinicius Borges ◽  
Daniel Rodrigues Nere ◽  
Leandro Carvalho Da Silva ◽  
Marília Gomes Caminha ◽  
Ervino Bleicher ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Multivariate Analysis ◽  
Vigna Unguiculata ◽  
Free Choice ◽  
Green Beans ◽  
Sources Of Resistance ◽  
High Genetic Diversity ◽  
Old Adult ◽  
Aphis Craccivora Koch ◽  
Cowpea Varieties

Evaluation of resistance of Vigna unguiculata L. landraces, used as green beans, to Aphis craccivora Koch was tested. Eleven landraces and standard genotypes were used in a free choice protocol: BR 17-Gurguéia, VITA 7 (susceptible), BRS Guariba and TVu 408P2 (resistant). A completely randomized blocks design was used with six replicates. Seedlings were infested with five six-day-old adult insects. The number of alive adults after 48 h and of alive nymphs after 96 h was evaluated. The results evidenced that there are high genetic diversity among the landraces, the antibiosis as the main mechanism of resistance. By means of multivariate analysis we suggest crosses among the commercial cultivar BRS Guariba and the most dissimilar varieties CCE-088, CCE-075, CCE-013 and CCE-062 to obtain superior hybrids.

scholarly journals Genetic Diversity among Cowpea (Vigna unguiculata (L.) Walp.) Landraces Suggests Central Mozambique as an Important Hotspot of Variation

Agronomy ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 1893
Author(s):  
Ana Maria Figueira Gomes ◽  
David Draper ◽  
Pedro Talhinhas ◽  
Paula Batista Santos ◽  
Fernanda Simões ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Gene Flow ◽  
Vigna Unguiculata ◽  
African Countries ◽  
High Genetic Diversity ◽  
Ecological Zones ◽  
Nuclear Microsatellite ◽  
Tropical Areas ◽  
Other World ◽  
High Level

Cowpea is a multiple-purpose drought-tolerant leguminous pulse crop grown in several dry tropical areas. Its domestication center is thought to be East or West Africa, where a high level of genetic diversity is apparently still found. However, detailed genetic information is lacking in many African countries, limiting the success of breeding programs. In this work, we assessed the genetic variation and gene flow in 59 Vigna unguiculata (cowpea) accessions from 10 landraces spanning across six agro-ecological zones of Mozambique, based on nuclear microsatellite markers. The results revealed the existence of high genetic diversity between the landraces, even in comparison to other world regions. Four genetic groups were found, with no specific geographic pattern, suggesting the presence of gene flow between landraces. In comparison, the two commercial varieties had lower values of genetic diversity, although still close to the ones found in local landraces. The high genetic diversity found in Mozambique sustains the importance of local genetic resources and farm protection to enhance genetic diversity in modern varieties of cowpea worldwide.

scholarly journals Sources of resistance to black aphid in cowpea varieties used as green grains

2020 ◽  
Vol 13 ◽  
pp. e902
Author(s):  
Vinicius Borges ◽  
Daniel Rodrigues Nere ◽  
Leandro Carvalho Da Silva ◽  
Marília Gomes Caminha ◽  
Ervino Bleicher ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Multivariate Analysis ◽  
Vigna Unguiculata ◽  
Free Choice ◽  
Green Beans ◽  
Sources Of Resistance ◽  
High Genetic Diversity ◽  
Old Adult ◽  
Aphis Craccivora Koch ◽  
Cowpea Varieties

Evaluation of resistance of Vigna unguiculata L. landraces, used as green beans, to Aphis craccivora Koch was tested. Eleven landraces and standard genotypes were used in a free choice protocol: BR 17-Gurguéia, VITA 7 (susceptible), BRS Guariba and TVu 408P2 (resistant). A completely randomized blocks design was used with six replicates. Seedlings were infested with five six-day-old adult insects. The number of alive adults after 48 h and of alive nymphs after 96 h was evaluated. The results evidenced that there are high genetic diversity among the landraces, the antibiosis as the main mechanism of resistance. By means of multivariate analysis we suggest crosses among the commercial cultivar BRS Guariba and the most dissimilar varieties CCE-088, CCE-075, CCE-013 and CCE-062 to obtain superior hybrids.

scholarly journals Genetic Diversity Studies in Seed Cowpea (Vigna unguiculata L. Walp) Genotypes

2021 ◽  
Vol 10 (10) ◽  
pp. 533-538
Author(s):  
A. R. Patil G. C. Shinde ◽  
V. R. Awari S. I. Tambe
Keyword(s):  
Genetic Diversity ◽  
Plant Height ◽  
Vigna Unguiculata ◽  
Genetic Divergence ◽  
Geographical Region ◽  
High Genetic Diversity ◽  
Geographic Diversity ◽  
Cluster Distance ◽  
Per Se ◽  
Diversity Studies

Thirty three seed cow genotypes (Vigna unguiculata L. WALP) collected from different geographical region were assessed for their genetic divergence based on ten characters during kharif-2020 at Post Graduate Farm, MPKV, Rahuri. The genotypes were grouped into six clusters which indicating high genetic diversity among them. It indicated that genetic diversity and geographic diversity are not related. The present study revealed that days to 50% flowering contributed maximum (34.09%) for divergence followed by plant height (32.58%), number of pods per plant (10.98%) and test weight (8.71%). Based on inter-cluster distance, intra-cluster distance and per se performance the genotypes viz., CP-04, CP-10, CP-10R, PCP-1411, CP-37, CP-10R,CP-39 and Phule Sonali are suggested to utilize for future breeding programme.

scholarly journals Subtype Characterization and Zoonotic Potential of Cryptosporidium felis in Cats in Guangdong and Shanghai, China

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 89
Author(s):  
Jiayu Li ◽  
Fuxian Yang ◽  
Ruobing Liang ◽  
Sheng Guo ◽  
Yaqiong Guo ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Genetic Characterization ◽  
Zoonotic Potential ◽  
Common Occurrence ◽  
High Genetic Diversity ◽  
Gp60 Gene ◽  
The Common ◽  
Subtyping Tool

Cryptosporidiumfelis is an important cause of feline and human cryptosporidiosis. However, the transmission of this pathogen between humans and cats remains controversial, partially due to a lack of genetic characterization of isolates from cats. The present study was conducted to examine the genetic diversity of C. felis in cats in China and to assess their potential zoonotic transmission. A newly developed subtyping tool based on a sequence analysis of the 60-kDa glycoprotein (gp60) gene was employed to identify the subtypes of 30 cat-derived C. felis isolates from Guangdong and Shanghai. Altogether, 20 C. felis isolates were successfully subtyped. The results of the sequence alignment showed a high genetic diversity, with 13 novel subtypes and 2 known subtypes of the XIXa subtype family being identified. The known subtypes were previously detected in humans, while some of the subtypes formed well-supported subclusters with human-derived subtypes from other countries in a phylogenetic analysis of the gp60 sequences. The results of this study confirmed the high genetic diversity of the XIXa subtype family of C. felis. The common occurrence of this subtype family in both humans and cats suggests that there could be cross-species transmission of C. felis.

scholarly journals Phylogeography of the Brittle Star Ophiura sarsii Lütken, 1855 (Echinodermata: Ophiuroidea) from the Barents Sea and East Atlantic

Diversity ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 40
Author(s):  
Evgeny Genelt-Yanovskiy ◽  
Yixuan Li ◽  
Ekaterina Stratanenko ◽  
Natalia Zhuravleva ◽  
Natalia Strelkova ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Barents Sea ◽  
Haplotype Diversity ◽  
Brittle Star ◽  
The Arctic ◽  
Coi Gene ◽  
High Genetic Diversity ◽  
Svalbard Archipelago ◽  
Mitochondrial Coi ◽  
The Barents Sea

Ophiura sarsii is a common brittle star species across the Arctic and Sub-Arctic regions of the Atlantic and the Pacific oceans. Ophiurasarsii is among the dominant echinoderms in the Barents Sea. We studied the genetic diversity of O.sarsii by sequencing the 548 bp fragment of the mitochondrial COI gene. Ophiurasarsii demonstrated high genetic diversity in the Barents Sea. Both major Atlantic mtDNA lineages were present in the Barents Sea and were evenly distributed between the northern waters around Svalbard archipelago and the southern part near Murmansk coast of Kola Peninsula. Both regions, and other parts of the O.sarsii range, were characterized by high haplotype diversity with a significant number of private haplotypes being mostly satellites to the two dominant haplotypes, each belonging to a different mtDNA clade. Demographic analyses indicated that the demographic and spatial expansion of O.sarsii in the Barents Sea most plausibly has started in the Bølling–Allerød interstadial during the deglaciation of the western margin of the Barents Sea.

scholarly journals Genetic Diversity of Leuconostoc mesenteroides Isolates from Traditional Montenegrin Brine Cheese

2021 ◽  
Vol 9 (8) ◽  
pp. 1612
Author(s):  
Werner Ruppitsch ◽  
Andjela Nisic ◽  
Patrick Hyden ◽  
Adriana Cabal ◽  
Jasmin Sucher ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Safety Evaluation ◽  
Gene Clusters ◽  
Northern Region ◽  
Genomic Diversity ◽  
High Genetic Diversity ◽  
Pathogenic Factors ◽  
Flavor Development ◽  
Genomic Characterization

In many dairy products, Leuconostoc spp. is a natural part of non-starter lactic acid bacteria (NSLAB) accounting for flavor development. However, data on the genomic diversity of Leuconostoc spp. isolates obtained from cheese are still scarce. The focus of this study was the genomic characterization of Leuconostoc spp. obtained from different traditional Montenegrin brine cheeses with the aim to explore their diversity and provide genetic information as a basis for the selection of strains for future cheese production. In 2019, sixteen Leuconostoc spp. isolates were obtained from white brine cheeses from nine different producers located in three municipalities in the northern region of Montenegro. All isolates were identified as Ln. mesenteroides. Classical multilocus sequence tying (MLST) and core genome (cg) MLST revealed a high diversity of the Montenegrin Ln. mesenteroides cheese isolates. All isolates carried genes of the bacteriocin biosynthetic gene clusters, eight out of 16 strains carried the citCDEFG operon, 14 carried butA, and all 16 isolates carried alsS and ilv, genes involved in forming important aromas and flavor compounds. Safety evaluation indicated that isolates carried no pathogenic factors and no virulence factors. In conclusion, Ln. mesenteroides isolates from Montenegrin traditional cheeses displayed a high genetic diversity and were unrelated to strains deposited in GenBank.

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