scholarly journals Caffeine reverses the unconsciousness produced by light anesthesia in the continued presence of isoflurane in rats

PLoS ONE ◽  
2020 ◽  
Vol 15 (11) ◽  
pp. e0241818
Author(s):  
Aaron P. Fox ◽  
Kyle R. Wagner ◽  
Vernon L. Towle ◽  
Kelvin G. Xie ◽  
Zheng Xie
Keyword(s):  
Heart Rate ◽  
Respiration Rate ◽  
Burst Suppression ◽  
Saline Control ◽  
Intracellular Camp ◽  
General Anesthetics ◽  
Control Respiration ◽  
Righting Reflex ◽  
Eeg Recordings ◽  
Circuit Function

Currently no drugs are employed clinically to reverse the unconsciousness induced by general anesthetics. Our previous studies showed that caffeine, when given near the end of an anesthesia session, accelerated emergence from isoflurane anesthesia, likely caused by caffeine’s ability to elevate intracellular cAMP levels and to block adenosine receptors. These earlier studies showed that caffeine did not rouse either rats or humans from deep anesthesia (≥ 1 minimum alveolar concentration, MAC). In this current crossover study, we examined whether caffeine reversed the unconsciousness produced by light anesthesia (< 1 MAC) in the continued presence of isoflurane. The primary endpoint of this study was to measure isoflurane levels at the time of recovery of righting reflex, which was a proxy for consciousness. Rats were deeply anesthetized with 2% isoflurane (~1.5 MAC) for 20 minutes. Subsequently, isoflurane was reduced to 1.2% for 10 minutes, then by 0.2% every 10 min; animals were monitored until the recovery of righting reflex occurred, in the continued presence of isoflurane. Respiration rate, heart rate and electroencephalogram (EEG) were monitored. Our results show that caffeine-treated rats recovered their righting reflex at a significantly higher inspired isoflurane concentration, corresponding to light anesthesia, than the same rats treated with saline (control). Respiration rate and heart rate increased initially after caffeine injection but were then unchanged for the rest of the anesthesia session. Deep anesthesia is correlated with burst suppression in EEG recordings. Our data showed that caffeine transiently reduced the burst suppression time produced by deep anesthesia, suggesting that caffeine altered neuronal circuit function but not to a point where it caused arousal. In contrast, under light anesthesia, caffeine shifted the EEG power to high frequency beta and gamma bands. These data suggest that caffeine may represent a clinically viable drug to reverse the unconsciousness produced by light anesthesia.

scholarly journals Effect of Dexmedetomidine on Perioperative Stress Response and Immune Function in Patients With Tumors

2020 ◽  
Vol 19 ◽  
pp. 153303382097754
Author(s):  
Lihong Zheng ◽  
Juan Zhao ◽  
Likun Zheng ◽  
Shuangfeng Jing ◽  
Xiaoting Wang
Keyword(s):  
Heart Rate ◽  
Stress Response ◽  
Mean Arterial Pressure ◽  
Arterial Pressure ◽  
Immune Function ◽  
Spontaneous Respiration ◽  
General Anesthetics ◽  
Ifn Γ ◽  
Group D ◽  
Perioperative Stress

Objective: This study aims to investigate the effect of dexmedetomidine on perioperative stress response and immune function in patients with tumors. Methods: Sixty patients who underwent selective radical gastrectomy for cancer were randomly divided into 3 groups: remifentanil group (group R), dexmedetomidine group (group D), and sufentanil group (group S). Remifentanil, dexmedetomidine, and sufentanil were used as general anesthetics. Endotracheal intubation and mechanical ventilation were performed after the spontaneous respiration disappeared. Then, the data were recorded, and blood samples were collected at all time points. Results: The heart rate significantly increased ( P < 0.05) at T1 in group S, and both heart rate and mean arterial pressure significantly increased ( P < 0.05) in group R when compared to group D. The heart rate significantly increased ( P < 0.05) at T2 in group S and group R. Furthermore, the heart rate significantly increased ( P < 0.05) at T3 and T4 in group S and group R. Intra-group comparison: The heart rate at T1–T4 and mean arterial pressure at T1–T4 significantly increased ( P < 0.05) in group S, and the heart rate at T1 and T4, and mean arterial pressure at T2–T4 significantly increased ( P < 0.05) in group R when compared to T0. The serum IL-6, IFN-γ, and β-EP significantly increased ( P < 0.05) at T0’ in group S and group R when compared to group D. Blood glucose, and serum IL-10, IFN-γ, and β-EP significantly increased ( P < 0.05), while IL-18 significantly decreased ( P < 0.05) at T1’ in group S and group R. Conclusion: Continuous infusion of dexmedetomidine in combination with the inhalation of sevoflurane is superior to sevoflurane + remifentanil or sufentanil in patients undergoing tumor surgery.

Stress level in wild harbour porpoises (Phocoena phocoena) during satellite tagging measured by respiration, heart rate and cortisol

2009 ◽  
Vol 89 (5) ◽  
pp. 885-892 ◽  
Author(s):  
I.G. Eskesen ◽  
J. Teilmann ◽  
B.M. Geertsen ◽  
G. Desportes ◽  
F. Riget ◽  
...  
Keyword(s):  
Heart Rate ◽  
Respiration Rate ◽  
Serum Cortisol ◽  
Phocoena Phocoena ◽  
Heart Rates ◽  
Stress Effects ◽  
Respiration Rates ◽  
Stressed Animal ◽  
Cortisol Levels ◽  
Satellite Tagging

During satellite tagging of harbour porpoises (Phocoena phocoena), heart rate, respiration rate and cortisol value were measured to evaluate stress effects during handling and tagging. Respiration rates were obtained using video recordings, heart rates were recorded and serum cortisol levels were analysed from blood samples. Differences in heart rates, respiration rates and cortisol levels before and during the tagging events were investigated. An overall significant decrease of 31.5% in respiration rate was found during the tagging event period, while mature porpoises respired significantly more often than immature individuals. Though significant differences in heart rates were found for some individuals, no general significant change for all animals was detected. We found no correlation between cortisol concentration and either heart rate or respiration rate, nor did we find any relationships between cortisol and month of year, sex and body length. As high individual variations occurred in response to tagging of harbour porpoises, it is not possible to give general advice based on the factors investigated, on how to reduce stress during handling. However, pouring water over the animal and lowering it into the water seem to stabilize a stressed animal. Therefore, general precaution and individual judgement based on experience is essential when handling wild harbour porpoises.

Stereoselective Loss of Righting Reflex in Rats by Isoflurane

2000 ◽  
Vol 93 (3) ◽  
pp. 837-843 ◽  
Author(s):  
Robert Dickinson ◽  
Ian White ◽  
William R. Lieb ◽  
Nicholas P. Franks
Keyword(s):  
Molecular Targets ◽  
Maximal Effect ◽  
General Anesthetics ◽  
General Anesthetic ◽  
Response Curves ◽  
Loss Of Righting Reflex ◽  
Large Numbers ◽  
Righting Reflex ◽  
Volatile Agents

Background Although it is accepted widely that optically active intravenous general anesthetics produce stereoselective effects in animals, the situation regarding volatile agents is confused. Conventional studies with scarce isoflurane enantiomers have been limited to small numbers of animals and produced conflicting results. By injecting these volatile enantiomers intravenously, however, it is possible to study large numbers of animals and obtain reliable results that can help to identify the molecular targets for isoflurane. Methods Pure isoflurane enantiomers were administered intravenously to rats after solubilization in a lipid emulsion. The ability of each enantiomer to produce a loss of righting reflex was determined as a function of dose, and quantal dose-response curves were constructed. In addition, sleep times were recorded with each enantiomer. Chiral gas chromatography was used to measure relative enantiomer concentrations in the brains of rats injected with racemic isoflurane. Results The S(+)-enantiomer was 40 +/- 8% more potent than the R(-)-enantiomer at producing a loss of righting reflex. The S(+)-enantiomer induced longer sleep times (by about 50%) than did the R(-)-enantiomer. Rats anesthetized by a dose of racemic isoflurane sufficient to achieve a half-maximal effect had essentially identical brain concentrations of the two enantiomers. Conclusions The S(+)-enantiomer of the general anesthetic isoflurane is significantly (P &lt; 0.001) more potent than the R(-)-enantiomer at causing a loss of righting reflex in rats. This confirms the view that isoflurane acts by binding to chiral sites. The observed degree of stereoselectivity provides a useful guide for ascertaining from in vitro experiments which molecular targets are most likely to play major roles in the loss of righting reflex caused by isoflurane.

scholarly journals A Pilot Study of the Evaluation of Using Antibiotics in Sepsis Patients in Palu, Indonesia

2020 ◽  
Vol 8 (B) ◽  
pp. 1036-1040
Author(s):  
Alwiyah Mukaddas ◽  
Tatat Rahmita Utami ◽  
Amelia Rumi
Keyword(s):  
Heart Rate ◽  
Pilot Study ◽  
Body Temperature ◽  
Respiration Rate ◽  
Descriptive Analysis ◽  
Kidney Injury ◽  
Baseline Heart Rate ◽  
Inclusion Criteria ◽  
Average Value ◽  
Gcs Score

BACKGROUND: Treatment therapy with antibiotics is one of the factors supporting success in the treatment of sepsis. AIM: This study aims to evaluate the use of antibiotics in patients with sepsis using parameters of the day of decline in body temperature, heart rate, respiration rate, changes in consciousness status, and comorbid factors. MATERIALS AND METHODS: The design of this study is pilot study with a retrospective approach on sample of 14 sepsis patients who met the inclusion criteria. Descriptive analysis using the univariate method for see changes in levels of body temperature, length of stay, respiration rate, heart rate, comorbid factors, and changes in the consciousness status. RESULTS: The results showed an average value for decline of body temperature after using antibiotics with a baseline of 38.47°C–37.87°C, heart rate shows the average value from baseline heart rate after using antibiotics from baseline of 110.8 bpm to 88.4 bpm, the respiration rate shows the average value for the respiration rate after using antibiotics from baseline of 30.8 x/min to 22.1 x/min, Glasgow Coma Scale (GCS) score showed an average value after using antibiotics from baseline 9 to 7, on comorbid factors showing six patients with one comorbid and eight patients with more than 1 comorbid factor. CONCLUSION: The study concluded that body temperature is still in the category of fever, heart rate, and respiration rate which are the normal category, patient consciousness is still at the level of somnolence even though patient’s GCS score has decreased, and patients with one factor of comorbidities are faster in death because they have a fatal type of comorbid such as acute of hepatitis, coma hepaticum, and acute kidney injury.

scholarly journals Accurate Estimation of Heart and Respiration Rates Based on an Optical Fiber Sensor Using Adaptive Regulations and Statistical Classifications Spectrum Analysis

2021 ◽  
Vol 3 ◽  
Author(s):  
Rongjian Zhao ◽  
Lidong Du ◽  
Zhan Zhao ◽  
Xianxiang Chen ◽  
Jie Sun ◽  
...  
Keyword(s):  
Heart Rate ◽  
Individual Differences ◽  
Optical Fiber ◽  
Confidence Intervals ◽  
Respiration Rate ◽  
Spectrum Analysis ◽  
Optical Fiber Sensor ◽  
Fiber Sensor ◽  
Respiration Rates ◽  
The Mean

The aim of this work is to present a method for accurately estimating heart and respiration rates under different actual conditions based on a mattress which was integrated with an optical fiber sensor. During the estimation, a ballistocardiogram (BCG) signal, which was obtained from the optical fiber sensor, was used for extracting the heart rate and the respiration rate. However, due to the detrimental effects of the differential detector, self-interference, and variation of installation status of the sensor, the ballistocardiogram (BCG) signal was difficult to detect. In order to resolve the potential concerns of individual differences and body interferences, adaptive regulations and statistical classifications spectrum analysis were used in this paper. Experiments were carried out to quantify heart and respiration rates of healthy volunteers under different breathing and posture conditions. From the experimental results, it could be concluded that (1) the heart rates of 40–150 beats per minute (bpm) and respiration rates of 10–20 breaths per minute (bpm) were measured for individual differences; (2) for the same individuals under four different posture contacts, the mean errors of heart rates were separately 1.60 ± 0.98 bpm, 1.94 ± 0.83 bpm, 1.24 ± 0.59 bpm, and 1.06 ± 0.62 bpm, in contrast, the mean errors of the polar beat device were 1.09 ± 0.96 bpm, 1.44 ± 0.99 bpm, and 1.78 ± 0.94 bpm. Furthermore, the experimental results were validated by conventional counterparts which used skin-contacting electrodes as their measurements. It was reported that the heart rate was 0.26 ± 2.80 bpm in 95% confidence intervals (± 1.96SD) in comparison with Philips sure-signs VM6 medical monitor, and the respiration rate was 0.41 ± 1.49 bpm in 95% confidence intervals (± 1.96SD) in comparison with ECG-derived respiratory (EDR) measurements for respiration rates. It was indicated that the developed system using adaptive regulations and statistical classifications spectrum analysis performed better and could easily be used under complex environments.

scholarly journals PHYSIOLOGICAL STATUS, BLOOD PROFILE AND BODY COMPOSITION OF SHEEP FED WITH CA-SAPONIFIED LEMURU OIL COATED BY HERBS

2015 ◽  
Vol 39 (2) ◽  
pp. 116
Author(s):  
Dewi Apri Astuti ◽  
Asep Sudarman
Keyword(s):  
Heart Rate ◽  
Body Composition ◽  
Respiration Rate ◽  
Body Fat ◽  
Rectal Temperature ◽  
Zingiber Officinale ◽  
Physiological Status ◽  
Total Body Fat ◽  
Total Body ◽  
Blood Profiles

<p>This study was aimed to evaluate the physiological status, blood profiles and body composition of sheep fed with Ca-saponified lemuru oil coated by herbs. Twenty fat-tailed sheep (av. BW 23±1.2 kg) were used in this experiment by using Completely Randomized Design with five treatments ration and four replications of each. Sheep fed with concentrate containing 3% Ca-saponified lemuru oil and king grass (1:1) ad libitum. Treatments were control diet without herbs (R1); Ca-saponified lemuru oil coated by curcumae domestica (R2); coated by Zingiber officinale Rosc (R3); coated by Eugenia polyantha (R4) and coated by Pluchea indica Less (R5). Data of physiological parameters were measured three times a day, in the morning, at noon and afternoon. Blood samples were collected at the end of the experiment through jugular vein, together with zero sample for Urea Space measurement. The variables observed were physiological data (heart rate, respiration rate, and rectal temperature), blood profiles (erythrocyte, hemoglobin, packed cell volume (PCV), leucocytes) and body composition (water, protein and fat) measured using Urea Space technique. Results of the study showed that sheep fed with Ca-saponified lemuru oil coated by herbs was not significantly different on heart rate, respiration rate and rectal temperature among treatments. Meanwhile, total leucocytes, neutrophil cell, and lymphocytes significantly increased (P&lt;0.05) by the treatment. Body composition percentage (water, protein and fat) were same in all treatments, except the total body fat and energy retained. In conclusion, supplementation of 3% Ca-saponified lemuru oil coated by Curcumae domestica, Zingiber officinale Rosc, Eugenia polyantha and Pluchea indica Less in fat-tailed sheep had no effect on physiological parameters, but improved the leucocyte and neutrophil cells. Total body fat and energy retained lower compared to control treatment.</p><p>(Key words: Body composition, Ca-saponified, Herbs, Lemuru oil, Urea space)</p>

25. COMPUTER ANALYSIS OF LONG-TERM RECORDS OF HEART RATE AND RESPIRATION RATE IN NEWBORN INFANTS

1970 ◽  
Vol 59 (s206) ◽  
pp. 144-146 ◽  
Author(s):  
I. VÄLIMÄKI ◽  
P. A. TARLO ◽  
K. E. SCOTT ◽  
V. C. UNITE ◽  
P. M. RAUTAHARJU
Keyword(s):  
Heart Rate ◽  
Respiration Rate ◽  
Computer Analysis ◽  
Newborn Infants

EFFECTS OF LOW FLUCTUATING TEMPERATURES ON FARM ANIMALS: I. INFLUENCE OF AMBIENT AIR TEMPERATURE ON THE RESPIRATION RATE, HEART RATE, AND RECTAL TEMPERATURE OF LACTATING HOLSTEIN-FRIESIAN COWS

1958 ◽  
Vol 38 (1) ◽  
pp. 10-22 ◽  
Author(s):  
M. A. MacDonald ◽  
J. M. Bell
Keyword(s):  
Heart Rate ◽  
Respiration Rate ◽  
Air Temperature ◽  
Rectal Temperature ◽  
Ambient Air ◽  
Farm Animals ◽  
Measurement Unit ◽  
Ambient Air Temperature ◽  
Air Temperatures ◽  
Holstein Friesian

This report presents data on the effect of low fluctuating ambient air temperatures on the rectal temperature, heart rate, and respiration rate in lactating Holstein-Friesian cows.Daily minimum ambient air temperature (DMAAT) inside the uninsulated University of Saskatchewan loose-housing shed ranged from −5° F. to 38° F. As ambient temperature decreased, rectal temperature and heart rate increased, while respiration rate decreased. Levels of significance were 10, 9, and 1 per cent for regressions of rectal temperature, heart rate, and respiration rate, respectively, on DMAAT. Levels of significance were 7, 10, and 1 per cent for regressions of rectal temperature, heart rate, and respiration rate, respectively, on degree hours per day (d-h/day). Degree hours per day is a measurement unit developed by the authors and is based on time and difference in degrees from 50° F.Change in rectal temperature and heart rate were not significantly (P = >.05) correlated with either change in d-h/day or change in DMAAT. Change in respiration rate was significantly (P = <.02) and negatively correlated with change in d-h/day and significantly (P = <.06) and positively correlated with change in DMAAT.Heart rate, rectal temperature, and respiration rate were not significantly correlated with each other. However, change in respiration rate was positively correlated with change in rectal temperature (P = <.03).While the influence of low temperatures resulted in small changes in these physiological characteristics compared to those experienced elsewhere in high temperature zones, it cannot be concluded that lactating cows were entirely free of thermal stress at temperatures as low as 0° F.

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