Response of human oral mucosal epithelial cells to different storage temperatures: A structural and transcriptional study

Purpose Seeking to improve the access to regenerative medicine, this study investigated the structural and transcriptional effects of storage temperature on human oral mucosal epithelial cells (OMECs). Methods Cells were stored at four different temperatures (4°C, 12°C, 24°C and 37°C) for two weeks. Then, the morphology, cell viability and differential gene expression were examined using light and scanning electron microscopy, trypan blue exclusion test and TaqMan gene expression array cards, respectively. Results Cells stored at 4°C had the most similar morphology to non-stored controls with the highest viability rate (58%), whereas the 37°C group was most dissimilar with no living cells. The genes involved in stress-induced growth arrest (GADD45B) and cell proliferation inhibition (TGFB2) were upregulated at 12°C and 24°C. Upregulation was also observed in multifunctional genes responsible for morphology, growth, adhesion and motility such as EFEMP1 (12°C) and EPHA4 (4°C–24°C). Among genes used as differentiation markers, PPARA and TP53 (along with its associated gene CDKN1A) were downregulated in all temperature conditions, whereas KRT1 and KRT10 were either unchanged (4°C) or downregulated (24°C and 12°C; and 24°C, respectively), except for upregulation at 12°C for KRT1. Conclusions Cells stored at 12°C and 24°C were stressed, although the expression levels of some adhesion-, growth- and apoptosis-related genes were favourable. Collectively, this study suggests that 4°C is the optimal storage temperature for maintenance of structure, viability and function of OMECs after two weeks.

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Non-redundant inhibitor of differentiation (Id) gene expression and function in human prostate epithelial cells

The Prostate ◽

10.1002/pros.20366 ◽

2006 ◽

Vol 66 (9) ◽

pp. 921-935 ◽

Cited By ~ 31

Author(s):

Ananthi J. Asirvatham ◽

Michelle A. Schmidt ◽

Jaideep Chaudhary

Keyword(s):

Gene Expression ◽

Epithelial Cells ◽

Human Prostate ◽

Prostate Epithelial Cells ◽

And Function

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Topography-dependent gene expression and function of common cell archetypes in large and small porcine airways

10.1101/2021.03.16.435690 ◽

2021 ◽

Author(s):

Alejandro A. Pezzulo ◽

Andrew L. Thurman ◽

Xiaopeng Li ◽

Raul Villacreses ◽

Wenjie Yu ◽

...

Keyword(s):

Gene Expression ◽

Epithelial Cells ◽

Airway Epithelial Cells ◽

Small Airway ◽

Small Airways ◽

Sequencing Data ◽

Airway Epithelia ◽

Large Airways ◽

And Function ◽

Airway Cells

SummaryThe small airways of humans are affected early in several lung diseases. However, because they are relatively inaccessible, little is known about the epithelial cells that line these airways. We performed a single cell RNA-seq census of small and large airways of wild-type pigs and pigs with disrupted cystic fibrosis transmembrane conductance regulator (CFTR) gene. The sequencing data showed that small airway epithelia had similar major cell types as large airways but no ionocytes; moreover, lack ofCFTRexpression had minimal effect on the transcriptome. Small airway epithelial cells expressed a different transcriptome than large airway cells. Quantitative immunohistochemistry showed that small airway basal cells participate in epithelial barrier function. Finally, sequencing data and in vitro electrophysiologic studies suggest that small airway epithelia have a water and ion transport advantage. Our data highlight the archetypal nature of basal, secretory, and ciliated airway cells with location-dependent gene expression and function.

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Curcumin Down-Regulates Toll-Like Receptor-2 Gene Expression and Function in Human Cystic Fibrosis Bronchial Epithelial Cells

Biological and Pharmaceutical Bulletin ◽

10.1248/bpb.b18-00928 ◽

2019 ◽

Vol 42 (3) ◽

pp. 489-495

Author(s):

Niraj Chaudhary ◽

Keiko Ueno-Shuto ◽

Tomomi Ono ◽

Yuko Ohira ◽

Kenji Watanabe ◽

...

Keyword(s):

Gene Expression ◽

Cystic Fibrosis ◽

Epithelial Cells ◽

Bronchial Epithelial Cells ◽

Toll Like Receptor ◽

Bronchial Epithelial ◽

Toll Like Receptor 2 ◽

And Function

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Noninvasive stool-based detection of infant gastrointestinal development using gene expression profiles from exfoliated epithelial cells

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.00004.2010 ◽

2010 ◽

Vol 298 (5) ◽

pp. G582-G589 ◽

Cited By ~ 49

Author(s):

Robert S. Chapkin ◽

Chen Zhao ◽

Ivan Ivanov ◽

Laurie A. Davidson ◽

Jennifer S. Goldsby ◽

...

Keyword(s):

Gene Expression ◽

Epithelial Cells ◽

Transcriptional Activation ◽

Expression Profiles ◽

Gene Expression Profiles ◽

Coefficient Of Determination ◽

Data Sets ◽

Developmentally Regulated ◽

Linear Discriminant ◽

And Function

We have developed a novel molecular methodology that utilizes stool samples containing intact sloughed epithelial cells to quantify intestinal gene expression profiles in the developing human neonate. Since nutrition exerts a major role in regulating neonatal intestinal development and function, our goal was to identify gene sets (combinations) that are differentially regulated in response to infant feeding. For this purpose, fecal mRNA was isolated from exclusively breast-fed ( n = 12) and formula-fed ( n = 10) infants at 3 mo of age. Linear discriminant analysis was successfully used to identify the single genes and the two- to three-gene combinations that best distinguish the feeding groups. In addition, putative “master” regulatory genes were identified using coefficient of determination analysis. These results support our premise that mRNA isolated from stool has value in terms of characterizing the epigenetic mechanisms underlying the developmentally regulated transcriptional activation/repression of genes known to modulate gastrointestinal function. As larger data sets become available, this methodology can be extended to validation and, ultimately, identification of the main nutritional components that modulate intestinal maturation and function.

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