scholarly journals Combining SIMS and mechanistic modelling to reveal nutrient kinetics in an algal-bacterial mutualism

PLoS ONE ◽  
2021 ◽  
Vol 16 (5) ◽  
pp. e0251643
Author(s):  
Hannah Laeverenz Schlogelhofer ◽  
François J. Peaudecerf ◽  
Freddy Bunbury ◽  
Martin J. Whitehouse ◽  
Rachel A. Foster ◽  
...  
Keyword(s):  
Secondary Ion Mass Spectrometry ◽  
Heterotrophic Bacterium ◽  
Microbial Interactions ◽  
Vitamin B ◽  
Cell Level ◽  
Mechanistic Modelling ◽  
Novel Approach ◽  
Algal Photosynthesis ◽  
Microbial Systems ◽  
Secondary Ion

Microbial communities are of considerable significance for biogeochemical processes, for the health of both animals and plants, and for biotechnological purposes. A key feature of microbial interactions is the exchange of nutrients between cells. Isotope labelling followed by analysis with secondary ion mass spectrometry (SIMS) can identify nutrient fluxes and heterogeneity of substrate utilisation on a single cell level. Here we present a novel approach that combines SIMS experiments with mechanistic modelling to reveal otherwise inaccessible nutrient kinetics. The method is applied to study the onset of a synthetic mutualistic partnership between a vitamin B12-dependent mutant of the alga Chlamydomonas reinhardtii and the B12-producing, heterotrophic bacterium Mesorhizobium japonicum, which is supported by algal photosynthesis. Results suggest that an initial pool of fixed carbon delays the onset of mutualistic cross-feeding; significantly, our approach allows the first quantification of this expected delay. Our method is widely applicable to other microbial systems, and will contribute to furthering a mechanistic understanding of microbial interactions.

scholarly journals Combining SIMS and mechanistic modelling to reveal nutrient kinetics in an algal-bacterial mutualism

2019 ◽  
Author(s):  
Hannah Laeverenz Schlogelhofer ◽  
François J. Peaudecerf ◽  
Freddy Bunbury ◽  
Martin J. Whitehouse ◽  
Rachel A. Foster ◽  
...  
Keyword(s):  
Secondary Ion Mass Spectrometry ◽  
Mechanistic Model ◽  
Heterotrophic Bacterium ◽  
Microbial Interactions ◽  
Vitamin B ◽  
Mesorhizobium Loti ◽  
Novel Approach ◽  
Algal Photosynthesis ◽  
Microbial Systems ◽  
Secondary Ion

AbstractMicrobial communities are of considerable significance for biogeochemical processes, for the health of both animals and plants, and for biotechnological purposes. A key feature of the interactions between microbes is the exchange of nutrients between cells. Isotope labelling followed by analysis with secondary ion mass spectrometry (SIMS) can identify nutrient fluxes and heterogeneity of substrate utilisation on a single cell level. Here we present a novel approach that combines SIMS with a mechanistic model to reveal otherwise inaccessible nutrient kinetics. The method is applied to study the onset of a synthetic mutualistic partnership between a vitamin B12-dependent mutant of the alga Chlamydomonas reinhardtii and the B12-producing, heterotrophic bacterium Mesorhizobium loti, which is supported by algal photosynthesis. Results show that an initial pool of fixed carbon delays the onset of mutualistic cross-feeding, and the model allows quantification of this delay. Our method is widely applicable to other microbial systems, and will contribute to furthering a mechanistic understanding of microbial interactions.

scholarly journals Stable Isotope Probing of RNA Combining Phylogenetic Microarrays and High Resolution Secondary Ion Mass Spectrometry to Link Composition and Function in Microbial Systems

2010 ◽  
Vol 16 (S2) ◽  
pp. 426-427
Author(s):  
X Mayali ◽  
PK Weber ◽  
EL Brodie ◽  
S Mabery ◽  
PD Hoeprich ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Stable Isotope ◽  
Secondary Ion Mass Spectrometry ◽  
Stable Isotope Probing ◽  
Ion Mass Spectrometry ◽  
And Function ◽  
Microbial Systems ◽  
Secondary Ion

Extended abstract of a paper presented at Microscopy and Microanalysis 2010 in Portland, Oregon, USA, August 1 – August 5, 2010.

scholarly journals Subcellular tracking reveals the location of dimethylsulfoniopropionate in microalgae and visualises its uptake by marine bacteria

eLife ◽  
2017 ◽  
Vol 6 ◽  
Author(s):  
Jean-Baptiste Raina ◽  
Peta L Clode ◽  
Soshan Cheong ◽  
Jeremy Bougoure ◽  
Matt R Kilburn ◽  
...  
Keyword(s):  
Large Scale ◽  
Secondary Ion Mass Spectrometry ◽  
Sulfur Cycle ◽  
Cell Level ◽  
Surface Ocean ◽  
Degrading Bacteria ◽  
First Time ◽  
Secondary Ion ◽  
Stable Sulfur Isotope ◽  
Inorganic Sulfate

Phytoplankton-bacteria interactions drive the surface ocean sulfur cycle and local climatic processes through the production and exchange of a key compound: dimethylsulfoniopropionate (DMSP). Despite their large-scale implications, these interactions remain unquantified at the cellular-scale. Here we use secondary-ion mass spectrometry to provide the first visualization of DMSP at sub-cellular levels, tracking the fate of a stable sulfur isotope (34S) from its incorporation by microalgae as inorganic sulfate to its biosynthesis and exudation as DMSP, and finally its uptake and degradation by bacteria. Our results identify for the first time the storage locations of DMSP in microalgae, with high enrichments present in vacuoles, cytoplasm and chloroplasts. In addition, we quantify DMSP incorporation at the single-cell level, with DMSP-degrading bacteria containing seven times more 34S than the control strain. This study provides an unprecedented methodology to label, retain, and image small diffusible molecules, which can be transposable to other symbiotic systems.

A novel approach for 3D reconstruction of mice full-grown oocytes by time-of-flight secondary ion mass spectrometry

2019 ◽  
Vol 412 (2) ◽  
pp. 311-319 ◽  
Author(s):  
Alexander Gulin ◽  
Victor Nadtochenko ◽  
Alyona Solodina ◽  
Maria Pogorelova ◽  
Artem Panait ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
3D Reconstruction ◽  
Secondary Ion Mass Spectrometry ◽  
Time Of Flight ◽  
Novel Approach ◽  
Ion Mass Spectrometry ◽  
Secondary Ion

Aspects of high-resolution imaging with a scanning ion microprobe

1986 ◽  
Vol 44 ◽  
pp. 750-751
Author(s):  
R. Levi-Setti ◽  
J. M. Chabala ◽  
Y. L. Wang
Keyword(s):  
Metal Ion ◽  
Secondary Ion Mass Spectrometry ◽  
Chromatic Aberration ◽  
Ion Source ◽  
Ion Microprobe ◽  
Emission Pattern ◽  
Intrinsic Resolution ◽  
Resolution Imaging ◽  
20 Nm ◽  
Secondary Ion

We have shown the feasibility of 20 nm lateral resolution in both topographic and elemental imaging using probes of this size from a liquid metal ion source (LMIS) scanning ion microprobe (SIM). This performance, which approaches the intrinsic resolution limits of secondary ion mass spectrometry (SIMS), was attained by limiting the size of the beam defining aperture (5μm) to subtend a semiangle at the source of 0.16 mr. The ensuing probe current, in our chromatic-aberration limited optical system, was 1.6 pA with Ga+ or In+ sources. Although unique applications of such low current probes have been demonstrated,) the stringent alignment requirements which they imposed made their routine use impractical. For instance, the occasional tendency of the LMIS to shift its emission pattern caused severe misalignment problems.

How SIMS microscopy can be used in medicine

1992 ◽  
Vol 50 (2) ◽  
pp. 1602-1603
Author(s):  
Philippe Fragu
Keyword(s):  
Mass Spectrometry ◽  
Biomedical Applications ◽  
Secondary Ion Mass Spectrometry ◽  
Chemical Elements ◽  
Biological Applications ◽  
The Past ◽  
Potential Source ◽  
Secondary Ion ◽  
Chemical Integrity ◽  
Scientific Endeavour

The identification, localization and quantification of intracellular chemical elements is an area of scientific endeavour which has not ceased to develop over the past 30 years. Secondary Ion Mass Spectrometry (SIMS) microscopy is widely used for elemental localization problems in geochemistry, metallurgy and electronics. Although the first commercial instruments were available in 1968, biological applications have been gradual as investigators have systematically examined the potential source of artefacts inherent in the method and sought to develop strategies for the analysis of soft biological material with a lateral resolution equivalent to that of the light microscope. In 1992, the prospects offered by this technique are even more encouraging as prototypes of new ion probes appear capable of achieving the ultimate goal, namely the quantitative analysis of micron and submicron regions. The purpose of this review is to underline the requirements for biomedical applications of SIMS microscopy.Sample preparation methodology should preserve both the structural and the chemical integrity of the tissue.

Advances in imaging SIMS studies of stained and BrdU-labelled human metaphase chromosomes

1995 ◽  
Vol 53 ◽  
pp. 932-933
Author(s):  
R. Levi-Setti ◽  
J. M. Chabala ◽  
R. Espinosa ◽  
M. M. Le Beau
Keyword(s):  
High Performance ◽  
Secondary Ion Mass Spectrometry ◽  
Analytical Sensitivity ◽  
Metaphase Chromosomes ◽  
Banding Patterns ◽  
Sector Mass Spectrometer ◽  
Staining Methods ◽  
The University ◽  
Secondary Ion ◽  
Better Than

We have shown previously that isotope-labelled nucleotides in human metaphase chromosomes can be detected and mapped by imaging secondary ion mass spectrometry (SIMS), using the University of Chicago high resolution scanning ion microprobe (UC SIM). These early studies, conducted with BrdU- and 14C-thymidine-labelled chromosomes via detection of the Br and 28CN- (14C14N-> labelcarrying signals, provided some evidence for the condensation of the label into banding patterns along the chromatids (SIMS bands) reminiscent of the well known Q- and G-bands obtained by conventional staining methods for optical microscopy. The potential of this technique has been greatly enhanced by the recent upgrade of the UC SIM, now coupled to a high performance magnetic sector mass spectrometer in lieu of the previous RF quadrupole mass filter. The high transmission of the new spectrometer improves the SIMS analytical sensitivity of the microprobe better than a hundredfold, overcoming most of the previous imaging limitations resulting from low count statistics.

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