scholarly journals PrintrLab incubator: A portable and low-cost CO2 incubator based on an open-source 3D printer architecture

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0251812
Author(s):  
Arunkumar Arumugam ◽  
Cole Markham ◽  
Saurabh S. Aykar ◽  
Barbara Van Der Pol ◽  
Paula Dixon ◽  
...  
Keyword(s):  
Open Source ◽  
Low Cost ◽  
Raspberry Pi ◽  
3D Printer ◽  
Software Application ◽  
G Code ◽  
Temperature Levels ◽  
Controller Board ◽  
Open Source Hardware ◽  
Hardware Designs

Growth in open-source hardware designs combined with the decreasing cost of high-quality 3D printers have supported a resurgence of in-house custom lab equipment development. Herein, we describe a low-cost (< $400), open-source CO2 incubator. The system is comprised of a Raspberry Pi computer connected to a 3D printer controller board that has controls for a CO2 sensor, solenoid valve, heater, and thermistors. CO2 is supplied through the sublimation of dry ice stored inside a thermos to create a sustained 5% CO2 supply. The unit is controlled via G-Code commands sent by the Raspberry Pi to the controller board. In addition, we built a custom software application for remote control and used the open-source Grafana dashboard for remote monitoring. Our data show that we can maintain consistent CO2 and temperature levels for over three days without manual interruption. The results from our culture plates and real-time PCR indicate that our incubator performed equally well when compared to a much more expensive commercial CO2 incubator. We have also demonstrated that the antibiotic susceptibility assay can be performed in this low-cost CO2 incubator. Our work also indicates that the system can be connected to incubator chambers of various chamber volumes.

scholarly journals The Incubot: A 3D Printer-Based Microscope for Long-Term Live Cell Imaging within a Tissue Culture Incubator

2020 ◽  
Author(s):  
George O. T. Merces ◽  
Conor Kennedy ◽  
Blanca Lenoci ◽  
Emmanuel G. Reynaud ◽  
Niamh Burke ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Experimental Design ◽  
Open Source ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Low Cost ◽  
Financial Burden ◽  
Live Cell ◽  
Raspberry Pi ◽  
3D Printer

AbstractCommercial live cell imaging systems represent a large financial burden to research groups, while current open source incubator microscopy systems lack adaptability and are sometimes inadequate for complex imaging experimentation. We present here a low-cost microscope designed for inclusion within a conventional tissue culture incubator. The build is constructed using an entry level 3D printer as the basis for the motion control system, with Raspberry Pi imaging and software integration, allowing for reflected, oblique, and fluorescence imaging of live cell monolayers. The open source nature of the design is aimed to facilitate adaptation by both the community at large and by individual researchers/groups. The development of an adaptable and easy-to-use graphic user interface (GUI) allows for the scientist to be at the core of experimental design. Simple modifications of the base GUI code, or generation of an entirely purpose-built script, will allow microscopists to place their experimental design as the priority, as opposed to designing experiments to fit their current equipment. The build can be constructed for a cost of roughly C1000 and thus serves as a low-cost and adaptable addition to the open source microscopy community.Abstract Figure

scholarly journals Monitoring System for Tracking a PV Generator in an Experimental Smart Microgrid: An Open-Source Solution

2021 ◽  
Vol 13 (15) ◽  
pp. 8182
Author(s):  
José María Portalo ◽  
Isaías González ◽  
Antonio José Calderón
Keyword(s):  
Open Source ◽  
Monitoring System ◽  
Smart Grids ◽  
Raspberry Pi ◽  
Operator Interface ◽  
Open Source Hardware ◽  
Real Time Information ◽  
And Storage ◽  
Time Information ◽  
Pv Generator

Smart grids and smart microgrids (SMGs) require proper monitoring for their operation. To this end, measuring, data acquisition, and storage, as well as remote online visualization of real-time information, must be performed using suitable equipment. An experimental SMG is being deployed that combines photovoltaics and the energy carrier hydrogen through the interconnection of photovoltaic panels, electrolyser, fuel cell, and load around a voltage bus powered by a lithium battery. This paper presents a monitoring system based on open-source hardware and software for tracking the temperature of the photovoltaic generator in such an SMG. In fact, the increases in temperature in PV modules lead to a decrease in their efficiency, so this parameter needs to be measured in order to monitor and evaluate the operation. Specifically, the developed monitoring system consists of a network of digital temperature sensors connected to an Arduino microcontroller, which feeds the acquired data to a Raspberry Pi microcomputer. The latter is accessed by a cloud-enabled user/operator interface implemented in Grafana. The monitoring system is expounded and experimental results are reported to validate the proposal.

Open-source hardware designs for MRI of mice, rats, and marmosets: Integrated animal holders and radiofrequency coils

2019 ◽  
Vol 312 ◽  
pp. 65-72 ◽  
Author(s):  
Kyle M. Gilbert ◽  
David J. Schaeffer ◽  
Joseph S. Gati ◽  
L. Martyn Klassen ◽  
Stefan Everling ◽  
...  
Keyword(s):  
Open Source ◽  
Radiofrequency Coils ◽  
Open Source Hardware ◽  
Hardware Designs

scholarly journals Computer Numerically Controlled Drawing Robot Based on Computer-Aided Design

2021 ◽  
Author(s):  
Antor Mahamudul Hashan ◽  
Abdullah Haidari ◽  
Srishti Saha ◽  
Titas Paul
Keyword(s):  
Open Source ◽  
Computer Aided Design ◽  
Low Cost ◽  
Rapid Development ◽  
Main Idea ◽  
Numerical Control ◽  
Computer Aided ◽  
Complex Circuits ◽  
Open Source Hardware ◽  
Aided Design

Due to the rapid development of technology, the use of numerically controlled machines in the industry is increasing. The main idea behind this paper is computer-aided design (CAD) based low-cost computer numerical control 2D drawing robot that can accurately draw complex circuits, diagrams, logos, etc. The system is created using open-source hardware and software, which makes it available at a low cost. The open-source LibreCAD application has been used for computer-aided design. Geometric data of a CAD model is converted to coordinate points using the python-based F-Engrave application. This system uses the Arduino UNO board as a signal generator of the universal g-code sender without compromising the performance. The proposed drawing robot is designed as a low-cost robot for educational purposes and aims to increase the student's interest in robotics and computer-aided design (CAD) skills to the next level. The drawing robot structure has been developed, and it meets the requirements of low cost with satisfactory experimental results.

scholarly journals Open-Source Electronics Platforms as Enabling Technologies for Smart Cities: Recent Developments and Perspectives

Electronics ◽  
2018 ◽  
Vol 7 (12) ◽  
pp. 404 ◽  
Author(s):  
Daniel Costa ◽  
Cristian Duran-Faundez
Keyword(s):  
Open Source ◽  
Smart City ◽  
Smart Cities ◽  
Low Cost ◽  
Raspberry Pi ◽  
Computational Power ◽  
Different Types ◽  
Recent Developments ◽  
Programmable Devices ◽  
Hardware Platforms

With the increasing availability of affordable open-source embedded hardware platforms, the development of low-cost programmable devices for uncountable tasks has accelerated in recent years. In this sense, the large development community that is being created around popular platforms is also contributing to the construction of Internet of Things applications, which can ultimately support the maturation of the smart-cities era. Popular platforms such as Raspberry Pi, BeagleBoard and Arduino come as single-board open-source platforms that have enough computational power for different types of smart-city applications, while keeping affordable prices and encompassing many programming libraries and useful hardware extensions. As a result, smart-city solutions based on such platforms are becoming common and the surveying of recent research in this area can support a better understanding of this scenario, as presented in this article. Moreover, discussions about the continuous developments in these platforms can also indicate promising perspectives when using these boards as key elements to build smart cities.

scholarly journals micrIO: an open-source autosampler and fraction collector for automated microfluidic input–output

Lab on a Chip ◽  
2020 ◽  
Vol 20 (1) ◽  
pp. 93-106 ◽  
Author(s):  
Scott A. Longwell ◽  
Polly M. Fordyce
Keyword(s):  
Open Source ◽  
Low Cost ◽  
Microfluidic Devices ◽  
Input Output ◽  
Fraction Collector ◽  
Open Source Hardware ◽  
Multiwell Plates

MicrIO is a low-cost, open-source hardware and software solution for automated sample input/output, bridging the gap between microfluidic devices and standard multiwell plates.

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