Independent of physical activity, volumetric muscle loss injury in a murine model impairs whole-body metabolism

Volumetric muscle loss (VML) injuries result in a non-recoverable loss of muscle tissue and function due to trauma or surgery. Reductions in physical activity increase the risk of metabolic comorbidities over time, and it is likely that VML may reduce whole-body activity. However, these aspects remain uncharacterized following injury. Our goal was to characterize the impact of VML on whole-body physical activity and metabolism, and to further investigate possible muscle-specific metabolic changes. Adult male C57Bl/6J (n = 28) mice underwent a standardized VML injury to the posterior compartment of the hind limb, or served as injury naïve controls. Mice underwent longitudinal evaluation of whole-body physical activity and metabolism in specialized cages up to three times over the course of 8 weeks. At terminal time points of 4- and 8-weeks post-VML in vivo muscle function of the posterior compartment was evaluated. Additionally, the gastrocnemius muscle was collected to understand histological and biochemical changes in the muscle remaining after VML. The VML injury did not alter the physical activity of mice. However, there was a noted reduction in whole-body metabolism and diurnal fluctuations between lipid and carbohydrate oxidation were also reduced, largely driven by lower carbohydrate utilization during active hours. Following VML, muscle-specific changes indicate a decreased proportion of fast (i.e., type IIb and IIx) and a greater proportion of slow (i.e., type I and IIa) fibers. However, there were minimal changes in the capillarity and metabolic biochemical activity properties of the gastrocnemius muscle, suggesting a miss-match in capacity to support the physiologic needs of the fibers. These novel findings indicate that following VML, independent of changes in physical activity, there is whole-body diurnal metabolic inflexibility. Supporting future investigations into the chronic and overlooked co-morbidities of VML injury.

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Interplay Between Whole Body Metabolism, Physical Activity, and Muscle Function Following Volumetric Muscle Loss Injury

The FASEB Journal ◽

10.1096/fasebj.2020.34.s1.05690 ◽

2020 ◽

Vol 34 (S1) ◽

pp. 1-1

Author(s):

Kyle A. Dalske ◽

Alec M. Basten ◽

Christiana J. Raymond-Pope ◽

Jarrod A. Call ◽

Sarah M Greising

Keyword(s):

Physical Activity ◽

Muscle Function ◽

Whole Body ◽

Muscle Loss ◽

Volumetric Muscle Loss ◽

Whole Body Metabolism

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The Effect of Repeated Whole-Body Cryotherapy on Sirt1 and Sirt3 Concentrations and Oxidative Status in Older and Young Men Performing Different Levels of Physical Activity

Antioxidants ◽

10.3390/antiox10010037 ◽

2020 ◽

Vol 10 (1) ◽

pp. 37

Author(s):

Gabriela Wojciak ◽

Jadwiga Szymura ◽

Zbigniew Szygula ◽

Joanna Gradek ◽

Magdalena Wiecek

Keyword(s):

Physical Activity ◽

Young Men ◽

Oxidative Status ◽

Whole Body ◽

Stress Index ◽

Blood Levels ◽

Long Distance ◽

Antioxidant Defence ◽

Whole Body Cryotherapy ◽

The Impact

Background: The activity of antioxidant enzymes and sirtuins (Sirt) decreases along with age, which is counteracted by aerobic training. Sirtuins increase antioxidant defence. Whole-body cryotherapy (WBC) increases total antioxidant capacity (TAC) in young men. The aim of our study was to assess the impact of 24 WBC treatments on the blood concentration of selected sirtuins and the level of antioxidant defence as well as oxidative stress index of training and non-training men depending on age. Methods: The study involved 40 males. In each group, there were 10 non-training older and young men (60 NTR and 20 NTR), and 10 older and young long-distance runners (60 TR, 20 TR). During an 8-week period, participants underwent 24 WBC treatments (3 min −130 °C), which were performed three times a week (Monday, Wednesday, Friday). The concentrations of Sirt1, Sirt3, TAC, total oxidative status and the activity of superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx) in the blood were determined before 1 WBC and after 1 WBC, 12 WBC and 24 WBC. Results: After 1 WBC, the activity of GPx and the concentration of Sirt1 and TAC in 60 TR and TAC in 60 NTR increased. After 12 WBC, the level of Sirt1 in 20 NTR and SOD in 20 TR increased. After 24 WBC, the level of Sirt1 increased in 60 TR and in 20 NTR, Sirt3 in 60 TR and SOD in 20 TR. Conclusions: Cryogenic temperatures increase blood levels of Sirt1 and Sirt3 and systemic antioxidant defence in men, but the effect is dependent on age, level of performed physical activity and the number of applied treatments.

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Impact of Physical Activity and Type of Cooking Oil Amongst Diabetes With Co-existing Hypertension Patients on Length of Stay and Cost: General Linear Model

10.21203/rs.3.rs-112180/v1 ◽

2020 ◽

Author(s):

Amit Sharma ◽

Ashish Baldi ◽

Dinesh Kumar Sharma

Keyword(s):

Physical Activity ◽

Diabetes Mellitus ◽

Length Of Stay ◽

Soybean Oil ◽

Olive Oil ◽

Daily Routine ◽

Type I ◽

High Physical Activity ◽

Cooking Oil ◽

The Impact

Abstract Background: Diabetes mellitus with co-existing hypertension contributes to increased morbidity and mortality. The study aimed to investigate the impact of the patients' physical activity status and the type of cooking oil consumed by patients in their daily routine on glycemic profile, lipid profile, the hypertensive profile of the patients, and the length of stay and overall cost of the treatment.Methods: A prospective observational study. All the patients who referred to the medicine department of the three different hospitals located in Moga, City Punjab and those who were hospitalized due to diabetes mellitus (type-I and type-II) with co-existing hypertension were asked to participate in the study.Results: The patients' mean age was found to be M= 53.85, SD= 11.54 years. Out of 1914 patients, 914 were male (47.8%); it was observed that the majority of the patients 525 (27.43%) in North India using butter or ghee- clarified butter as edible oil, followed by mustard oil 517 (27.01%) patients. About 345 (18.03%) of the patients consume soybean oil, whereas 226 (11.81%) of the patients like sunflower oil. Discussion: This study explored that cooking oil and physical activity are associated with length of stay in days & overall cost of the treatment, respectively. Our study results revealed that the type of oil compared with the treatment's overall cost was significant for olive oil, soybean oil, and groundnut oil.Conclusion: The study revealed that moderate and low physical activity increases the length of stay compared to high physical activity. The consumption of olive oil as a regular food habit in daily routine decreases patients' length of stay with diabetes with coexisting hypertension when doing high physical activity but increases the overall cost of treatment.

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Event-related potentials following exercise bouts of different intensity

Medicinski pregled ◽

10.2298/mpns0712531b ◽

2007 ◽

Vol 60 (11-12) ◽

pp. 531-535 ◽

Cited By ~ 1

Author(s):

Otto Barak ◽

Vesna Ivetic ◽

Danka Filipovic ◽

Nada Naumovic ◽

Damir Lukac ◽

...

Keyword(s):

Physical Activity ◽

Positive Impact ◽

Event Related Potentials ◽

Bicycle Ergometer ◽

Whole Body ◽

Functional Diagnostics ◽

Ergometer Exercise ◽

Related Potentials ◽

The Impact ◽

Bicycle Ergometer Exercise

Introduction. A number of articles on physical activity analyze the effects of acute bouts of physical exercise on the whole body. These experiments mainly include questionnaires and measurements of reaction time. The use of event-related potentials in laboratories for functional diagnostics is only of recent date. The aim of this experiment was to give insights into the impact of physical activity of different intensity on the amplitude and latency of P300 cognitive potentials. Material and methods. After recording cognitive event-related potentials in 17 young (21.6?1.07 yrs) healthy adults (at Fz and Cz), the participants underwent a controlled bicycle ergometer exercise. Each exercise lasted 10 minutes, with successive increase in the intensity to 60%, 75% and 90% of the maximum pulse rate and maintaining this level of intensity for six minutes. Immediately after each bout of exercise, event-related potentials were recorded. Results. The amplitude of the P300 wave, following exercise intensity at 75% of the maximum pulse (Pmax) (Fz 15.00?4.57; Cz 18.63?8.83 mV) was statistically higher (p<0.05) than the amplitude of the P300 at rest (Fz 11.21?4.15 mV; Cz 13.40?8.04 mV), at 60% (Fz 11.86?5.11 mV; Cz 14.54?8.06 mV) and at 90% of maximum pulse (Fz 13.26?4.73 mV; Cz 14.91?8.91 mV). There were no statistically significant differences (p>0.05) between amplitudes at 60% of Pmax and values obtained at rest and at 90% of Pmax. Also, no statistically significant differences were recorded (p>0.05) among the latencies of P300 recorded at rest (Fz 323.57?13.24 ms; Cz 323.57?13.24 ms) and at 60% of Pmax (Fz 321.14?22.38 ms; Cz 321.86?22.88 ms), at 75% of Pmax (Fz 321.50?16.67 ms; Cz 322.50?14.60 ms) and at 90% of Pmax (Fz 326.29?7.85 ms; Cz 325.43?7.63 ms). Discusssion and Conclusion. Physical activity has a positive impact on cognitive functions. At intermediate intensities, the amplitude of P300 increases, but at submaximal intensities it decreases to values obtained at rest. However, the latency of P300 did not show a statistically significant change after different intensities of exercise.

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Nutritional influences on age-related skeletal muscle loss

Proceedings of The Nutrition Society ◽

10.1017/s0029665113003698 ◽

2013 ◽

Vol 73 (1) ◽

pp. 16-33 ◽

Cited By ~ 57

Author(s):

Ailsa A. Welch

Keyword(s):

Skeletal Muscle ◽

Vitamin D ◽

Fruits And Vegetables ◽

Muscle Metabolism ◽

Ubiquitin Proteasome System ◽

Whole Body ◽

Muscle Loss ◽

Age Related ◽

Anti Oxidant ◽

Whole Body Metabolism

Age-related muscle loss impacts on whole-body metabolism and leads to frailty and sarcopenia, which are risk factors for fractures and mortality. Although nutrients are integral to muscle metabolism the relationship between nutrition and muscle loss has only been extensively investigated for protein and amino acids. The objective of the present paper is to describe other aspects of nutrition and their association with skeletal muscle mass. Mechanisms for muscle loss relate to imbalance in protein turnover with a number of anabolic pathways of which the mechanistic TOR pathway and the IGF-1–Akt–FoxO pathways are the most characterised. In terms of catabolism the ubiquitin proteasome system, apoptosis, autophagy, inflammation, oxidation and insulin resistance are among the major mechanisms proposed. The limited research associating vitamin D, alcohol, dietary acid–base load, dietary fat and anti-oxidant nutrients with age-related muscle loss is described. Vitamin D may be protective for muscle loss; a more alkalinogenic diet and diets higher in the anti-oxidant nutrients vitamin C and vitamin E may also prevent muscle loss. Although present recommendations for prevention of sarcopenia focus on protein, and to some extent on vitamin D, other aspects of the diet including fruits and vegetables should be considered. Clearly, more research into other aspects of nutrition and their role in prevention of muscle loss is required.

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Branched-chain Amino Acids: Catabolism in Skeletal Muscle and Implications for Muscle and Whole-body Metabolism

Frontiers in Physiology ◽

10.3389/fphys.2021.702826 ◽

2021 ◽

Vol 12 ◽

Author(s):

Gagandeep Mann ◽

Stephen Mora ◽

Glory Madu ◽

Olasunkanmi A. J. Adegoke

Keyword(s):

Skeletal Muscle ◽

Amino Acids ◽

Chronic Diseases ◽

Branched Chain Amino Acids ◽

Whole Body ◽

Management Options ◽

Impaired Metabolism ◽

Whole Body Metabolism ◽

Branched Chain ◽

The Impact

Branched-chain amino acids (BCAAs) are critical for skeletal muscle and whole-body anabolism and energy homeostasis. They also serve as signaling molecules, for example, being able to activate mammalian/mechanistic target of rapamycin complex 1 (mTORC1). This has implication for macronutrient metabolism. However, elevated circulating levels of BCAAs and of their ketoacids as well as impaired catabolism of these amino acids (AAs) are implicated in the development of insulin resistance and its sequelae, including type 2 diabetes, cardiovascular disease, and of some cancers, although other studies indicate supplements of these AAs may help in the management of some chronic diseases. Here, we first reviewed the catabolism of these AAs especially in skeletal muscle as this tissue contributes the most to whole body disposal of the BCAA. We then reviewed emerging mechanisms of control of enzymes involved in regulating BCAA catabolism. Such mechanisms include regulation of their abundance by microRNA and by post translational modifications such as phosphorylation, acetylation, and ubiquitination. We also reviewed implications of impaired metabolism of BCAA for muscle and whole-body metabolism. We comment on outstanding questions in the regulation of catabolism of these AAs, including regulation of the abundance and post-transcriptional/post-translational modification of enzymes that regulate BCAA catabolism, as well the impact of circadian rhythm, age and mTORC1 on these enzymes. Answers to such questions may facilitate emergence of treatment/management options that can help patients suffering from chronic diseases linked to impaired metabolism of the BCAAs.

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The Impact of Critical Illness on Skeletal Muscle Structure

10.1093/med/9780199653461.003.0034 ◽

2014 ◽

Author(s):

Catherine L Hough

Keyword(s):

Skeletal Muscle ◽

Critical Illness ◽

Thick Filament ◽

Muscle Membrane ◽

Type I ◽

Muscle Loss ◽

Muscle Structure ◽

Muscle Necrosis ◽

Skeletal Muscle Loss ◽

The Impact

Patients with critical illness are at risk of profound weakness and skeletal muscle loss, and recovery is marked by prolonged physical functional impairment in many survivors. Muscle and nerve abnormalities found in critically ill patients include loss of muscle mass, muscle membrane inexcitability, polyneuropathy, mitochondrial dysfunction with bioenergetic failure, as well as changes in skeletal muscle structure. The most common histological abnormalities are atrophy of both type I and II fibres and thick filament loss; muscle necrosis is less common. While recent studies have illuminated the pathogenesis of critical illness myopathy, additional high-quality translational research is needed to identify targets for therapeutic intervention.

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Losartan administration reduces fibrosis but hinders functional recovery after volumetric muscle loss injury

Journal of Applied Physiology ◽

10.1152/japplphysiol.00689.2014 ◽

2014 ◽

Vol 117 (10) ◽

pp. 1120-1131 ◽

Cited By ~ 37

Author(s):

Koyal Garg ◽

Benjamin T. Corona ◽

Thomas J. Walters

Keyword(s):

Skeletal Muscle ◽

Muscle Regeneration ◽

Muscle Function ◽

Collagen Type ◽

Force Production ◽

Collagen Type I ◽

Type I ◽

Muscle Loss ◽

Muscle Injuries ◽

Volumetric Muscle Loss

Losartan is a Food and Drug Administration approved antihypertensive medication that is recently emerging as an antifibrotic therapy. Previously, losartan has been successfully used to reduce fibrosis and improve both muscle regeneration and function in several models of recoverable skeletal muscle injuries, such as contusion and laceration. In this study, the efficacy of losartan treatment in reducing fibrosis and improving regeneration was determined in a Lewis rat model of volumetric muscle loss (VML) injury. VML has been defined as the traumatic or surgical loss of skeletal muscle with resultant functional impairment. It is among the top 10 causes for wounded service members to be medically retired from the military. This study shows that, after several weeks of recovery, VML injury results in little to no muscle regeneration, but is marked by persistent inflammation, chronic upregulation of profibrotic markers and extracellular matrix (i.e., collagen type I), and fat deposition at the defect site, which manifest irrecoverable deficits in force production. Losartan administration at 10 mg·kg−1·day−1was able to modulate the gene expression of fibrotic markers and was also effective at reducing fibrosis (i.e., the deposition of collagen type I) in the injured muscle. However, there were no improvements in muscle regeneration, and deleterious effects on muscle function were observed instead. We propose that, in the absence of regeneration, reduction in fibrosis worsens the ability of the VML injured muscle to transmit forces, which ultimately results in decreased muscle function.

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The Impact of Single-Cell Genomics on Adipose Tissue Research

International Journal of Molecular Sciences ◽

10.3390/ijms21134773 ◽

2020 ◽

Vol 21 (13) ◽

pp. 4773

Author(s):

Alana Deutsch ◽

Daorong Feng ◽

Jeffrey E. Pessin ◽

Kosaku Shinoda

Keyword(s):

Adipose Tissue ◽

Single Cell ◽

Energy Homeostasis ◽

Whole Body ◽

Diabetes Research ◽

Obesity And Diabetes ◽

Important Regulator ◽

Whole Body Metabolism ◽

Tissue Aging ◽

The Impact

Adipose tissue is an important regulator of whole-body metabolism and energy homeostasis. The unprecedented growth of obesity and metabolic disease worldwide has required paralleled advancements in research on this dynamic endocrine organ system. Single-cell RNA sequencing (scRNA-seq), a highly meticulous methodology used to dissect tissue heterogeneity through the transcriptional characterization of individual cells, is responsible for facilitating critical advancements in this area. The unique investigative capabilities achieved by the combination of nanotechnology, molecular biology, and informatics are expanding our understanding of adipose tissue’s composition and compartmentalized functional specialization, which underlie physiologic and pathogenic states, including adaptive thermogenesis, adipose tissue aging, and obesity. In this review, we will summarize the use of scRNA-seq and single-nuclei RNA-seq (snRNA-seq) in adipocyte biology and their applications to obesity and diabetes research in the hopes of increasing awareness of the capabilities of this technology and acting as a catalyst for its expanded use in further investigation.

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THE IMPACT OF REGULAR FOOTBALL TRAINING ON BONE TURNOVER MARKERS

Issues of Rehabilitation, Orthopaedics, Neurophysiology and Sport Promotion – IRONS ◽

10.19271/irons-000126-2020-33 ◽

2020 ◽

Vol 33 (33) ◽

pp. 31-40

Author(s):

Kacper Pajor ◽

Justyna Szpyt ◽

Agnieszka Turoń-Skrzypińska ◽

Iwona Rotter

Keyword(s):

Physical Activity ◽

Bone Turnover ◽

Bone Metabolism ◽

Bone Turnover Markers ◽

The Body ◽

Type I ◽

Physical Activity Monitoring ◽

Turnover Markers ◽

Football Training ◽

The Impact

Introduction The condition of the skeleton is important not only in the perspective of osteoporosis prevention, but also as a factor affecting the frequency of injuries excluding physical activity. Monitoring the impact of specific sports on osteoclasts and osteoblasts acitivity allows optimization of programming of physical activity limiting the risk of bone mineralization disorders. The review analyzes available papers on the impact of regular football training on skeletal physiology changes analyzed by measuring bone turnover markers in the body. Aim Determining the impact of regular football training on bone mass regulation by analyzing bone turnover markers. Material and methods PubMed and SPORTDiscus with Full Text databases were searched using the keyword combination “the name of team sport” + bone turnover. There is no clinical trials about bone turnover markers among handball, hockey, basketball and volleyball players in the available literature that meet the inclusion criteria, so the topic of the review was narrowed down to football (soccer). After applying the exclusion criteria, five studies were qualified. Results In the analyzed papers, the concentration of osteocalcin, N-terminal procollagen type I extension propeptide and C-terminal telopeptide of type I collagen in blood increased as a result of regular football training. In 3 papers statistically significant (p < 0.05) increases were noted. Conclusions Football training can stimulate bone metabolism, being an effective and attractive form of bone fracture prevention, regardless of your level of sport. Due to the limited availability of studies, there is a high need for further studies describing the impact of physical activity on bone metabolism.

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