scholarly journals A small step or a giant leap: Accounting for settlement delay and dispersal in restoration planning

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0256369
Author(s):  
Ana Rodriguez-Perez ◽  
Mark A. James ◽  
William G. Sanderson
Keyword(s):  
Larval Mortality ◽  
Larval Settlement ◽  
Control Treatment ◽  
Ostrea Edulis ◽  
Small Step ◽  
Restoration Planning ◽  
Dispersal Potential ◽  
Giant Leap ◽  
Restoration Strategies ◽  
Unknown Period

Understanding larval duration and hence dispersal potential of the European oyster Ostrea edulis is crucial to inform restoration strategies. Laval duration has an obligatory period of maturity to pediveliger (when larvae are ready to settle), but also an unknown period until metamorphosis is triggered by a settlement cue. The extent to which larvae can prolong the pediveliger period and delay metamorphosis has not been studied. Here we show that O. edulis larvae can delay metamorphosis for a period of 11 days, while retaining the capability to settle in high proportions when presented with a suitable settlement cue. O. edulis larvae are likely to be able to delay metamorphosis even further, since 80% of larvae in the control treatment were still alive when the experiment was terminated at day 14. The results indicate the ability of O. edulis larvae to more than double pelagic duration and probably further delay metamorphosis. We discuss these findings in the context of larval mortality, and the importance of O. edulis’ larval settlement requirements for dispersal potential, recruitment success and connectivity of restoration sites.

scholarly journals One Small Step for the Gram Stain, One Giant Leap for Clinical Microbiology

2016 ◽  
Vol 54 (6) ◽  
pp. 1416-1417 ◽  
Author(s):  
Richard B. Thomson
Keyword(s):  
Clinical Microbiology ◽  
Rapid Test ◽  
Error Rates ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory ◽  
Gram Stain ◽  
Small Step ◽  
Link Type ◽  
Giant Leap

The Gram stain is one of the most commonly performed tests in the clinical microbiology laboratory, yet it is poorly controlled and lacks standardization. It was once the best rapid test in microbiology, but it is no longer trusted by many clinicians. The publication by Samuel et al. (J. Clin. Microbiol. 54:1442–1447, 2016,http://dx.doi.org/10.1128/JCM.03066-15) is a start for those who want to evaluate and improve Gram stain performance. In an age of emerging rapid molecular results, is the Gram stain still relevant? How should clinical microbiologists respond to the call to reduce Gram stain error rates?

scholarly journals One small step for e-voting, one giant leap for democracy

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Marina Gorbatiuc
Keyword(s):  
Human Rights ◽  
Law Enforcement ◽  
International Organizations ◽  
The Internet ◽  
Mutual Understanding ◽  
Small Step ◽  
Law Enforcement Training ◽  
Republic Of Moldova ◽  
Giant Leap ◽  
The Republic

E-voting is the next step of the Republic of Moldova evolution. The article presents advantages of e-voting, all steps of using it, and its many types. Electronic voting refers to elections using electronic means. E-voting can be managed by phones, the Internet, private computer networks or special kiosks. Reasons for accepting this kind of voting are provided. The analyzed in the article OSCE/ODIHR’s (Office for Democratic Institutions and Human Rights) activities which are related to tolerance and non-discrimination are focus on the following areas: legislation; law enforcement training; monitoring, reporting on, and following up on responses to hate-motivated crimes and incidents; as well as educational activities to promote tolerance, respect, and mutual understanding. Conclusions from all ODIHR activities which are carried out in close co-ordination and co-operation with OSCE participating States, OSCE institutions and field operations, as well as with other international organizations are given.

One small step in frailty research, a giant leap in evidence based practice

2018 ◽  
Vol 16 (1) ◽  
pp. 1-3 ◽  
Author(s):  
João Apóstolo ◽  
Elzbieta Bobrowicz-Campos ◽  
Carol Holland ◽  
Antonio Cano
Keyword(s):  
Evidence Based Practice ◽  
Evidence Based ◽  
Small Step ◽  
Giant Leap

scholarly journals Cyclo(l-Pro–l-Leu) of Pseudomonas putida MCCC 1A00316 Isolated from Antarctic Soil: Identification and Characterization of Activity against Meloidogyne incognita

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 768 ◽  
Author(s):  
Yile Zhai ◽  
Zongze Shao ◽  
Minmin Cai ◽  
Longyu Zheng ◽  
Guangyu Li ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Pseudomonas Putida ◽  
Meloidogyne Incognita ◽  
High Performance ◽  
Larval Mortality ◽  
Control Treatment ◽  
Hatching Rate ◽  
Antarctic Soil ◽  
Egg Hatching ◽  
Flight Mass Spectrometry

Pseudomonas putida MCCC 1A00316 was originally isolated from an Antarctic soil and has demonstrated potential nematicidal activity. Thus, it has promising applications for the biological control of Meloidogyne incognita. The larval mortality and egg-hatching inhibition rates of M. incognita will increase with the rising concentration of culture filtrates of P. putida MCCC 1A00316 and the duration of exposure. Thus, this study aimed to separate, purify, and identify nematicidal compounds from P. putida MCCC 1A00316 and to validate their anti-M. incognita activities. Compounds were purified through silica gel column chromatography and thin-layer chromatography combined with high-performance liquid chromatography (HPLC). Structural identification was conducted through liquid chromatography time-of-flight mass spectrometry, 1H nuclear magnetic resonance (NMR) spectroscopy, 13C-NMR, and Marfey’s method. The isolated compounds were identified as cyclo(l-Pro–l-Leu) on the basis of the results of the above analyses and previously reported data. The effects of various concentrations of cyclo(l-Pro–l-Leu) on the mortality rates of second-stage juveniles (J2) of M. incognita were investigated. Results showed that HPLC-purified cyclo(l-Pro–l-Leu) displayed nematicidal activities. The mortality rate of M. incognita J2 reached 84.3% after 72 h of exposure to 67.5 mg/L cyclo(l-Pro–l-Leu). The lowest egg-hatching rate (9.74%) was observed after 8 days of incubation with 2000 mg/L cyclo(l-Pro–l-Leu). An egg-hatching rate of 53.11% was obtained under the control treatment (sterile distilled water). However, cyclo(l-Pro–l-Leu) did not elicit chemotaxis activity to M. incognita. This is the first work to investigate the anti-M. incognita characteristics of cyclo(l-Pro–l-Leu).

scholarly journals Quadruplet codons: One small step for a ribosome, one giant leap for proteins

BioEssays ◽  
2010 ◽  
Vol 32 (8) ◽  
pp. 650-654 ◽  
Author(s):  
Irene A. Chen ◽  
Michael Schindlinger
Keyword(s):  
Small Step ◽  
Giant Leap
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