scholarly journals Correlation of breast cancer microcirculation construction with tumor stem cells (CSCs) and epithelial-mesenchymal transition (EMT) based on contrast-enhanced ultrasound (CEUS)

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0261138
Author(s):  
Xiaoling Leng ◽  
Guofu Huang ◽  
Siyi Li ◽  
Miaomiao Yao ◽  
Jianbing Ding ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Histological Grade ◽  
Node Metastasis ◽  
Contrast Enhanced ◽  
Epithelial Phenotype ◽  
Cancer Tissues ◽  
Er Expression ◽  
E Cadherin

Objective This study is to explore the correlation between the contrast-enhanced ultrasound (CEUS) characteristics of breast cancer and the epithelial-mesenchyme transformation (EMT). Methods Totally 119 patients of breast cancer underwent CEUS. Tissues in the active area were collected and subjected to the immunohistochemical detection, PT-PCR and Western blot. Correlation analysis was conducted between the clinical pathological parameters and the CEUS indicators. Results The expression levels of CD44, N-cadherin, and β-catenin in breast cancer tissues were higher than those in adjacent tissues (P<0.05). However, the expression levels of CD24 and E-cadherin in breast cancer tissues were lower than those in adjacent tissues (P<0.05). There was no significant difference in E-cadherin mRNA and Vimentin levels between cancer and adjacent tissues (P>0.05). The expressions were up-regulated in the CSCs, with higher histological grade, lymph node metastasis, and negative estrogen receptor (ER) expression. Smaller breast tumors, with no lymph node metastasis, lower clinical stage, and positive ER expression, tended to exhibit the up-regulated epithelial phenotype. Breast tumors, with high histological grade, lymph node metastasis, high clinical staging grade, and negative ER expression, tended to exhibit the up-regulated interstitial phenotype. The peak intensity of the time-intensity curve (TIC) for the CEUS was positively correlated with the CSC marker CD44 and the interstitial phenotype marker N-cadherin. The starting time of enhancement was negatively correlated with the N-cadherin. Area under the curve was positively correlated with the expression of CD44 and N-cadherin, while negatively correlated with the epithelial phenotype marker β-catenin. The time to peak was negatively correlated with the interstitial phenotypes Vimentin and N-cadherin, with no correlation with the E-cadherin or β-catenin. Conclusion Breast cancers show the enlarged lesions after enlargement and perfusion defect for the CEUS. The fast-in pattern, high enhancement, and high perfusion in the TIC are correlated with the CSCs and EMT expressions, suggesting poor disease prognosis.

Characteristics of medullary breast carcinoma compared with invasive ductal carcinoma.

2012 ◽  
Vol 30 (27_suppl) ◽  
pp. 20-20
Author(s):  
Inhye Park ◽  
Jiyoung Kim ◽  
Se-Kyung Lee ◽  
Min-Young Choi ◽  
Su Yeon Bae ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Prognostic Factors ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Invasive Ductal Carcinoma ◽  
Tumor Size ◽  
Ductal Carcinoma ◽  
Histological Grade ◽  
Clinicopathologic Features ◽  
Node Metastasis

20 Background: Medullary carcinoma (MC) represents a rare breast cancer subtype associated with a rather favorable prognosis compared with invasive ductal carcinoma (IDC). It is characterized by the high-grade structure and lymphocytic infiltration, hemorrhagic necrosis. The purpose of this study is to compare the clinicopathologic characteristics and outcome of MC to IDC. Methods: We retrospectively reviewed the medical records of patients with invasive breast cancer managed with operation at Samsung Medical Center in Korea from January 1995 to June 2010 except patients diagnosed with ductal carcinoma in situ, patients with distant metastasis at diagnosis or neoadjuvant chemotherapy. 52 cases were identified with MC; 5,716 patients with IDC. The clinicopathologic features, disease-free survival (DFS) and overall survival (OS) for patients with MC were compared with those of the IDC patients. Results: The medullary group presented at younger age (43.9 ± 8.8 vs 47.7 ± 9.9, p=0.006). Also the medullary group was significantly associated with higher histological grade (poor; 80.0 vs 38.3%, p=0.003) and nuclear grade (grade3; 82.8 vs 41.7%, p<0.001) as well as negative ER (84.8 vs 31.0%, p<0.001) and PR status (91.3 vs 38.8%, p<0.001) regarded as poor prognostic factors. But lymphatic invasion was rare (0.0 vs 29.8%, p<0.001) and N stage was low (N0; 86.5 vs 58.4%, p<0.001). The DFS and OS were not significantly different between the medullary and IDC groups. (5-yr DFS : 88.0 vs 89.2 %, p=0.917, 5-yr OS : 94.4 vs 93.4%, p=0.502) In multivariable analysis, factors associated with DFS and OS included nuclear grade, histological grade, tumor size, lymph node metastasis, ER/PR/C-erbB2 status, chemotherapy and hormone therapy. When adjusting for other factors, histological type itself did not show significant difference from IDC in DFS and OS. Conclusions: Despite MC present specific clinicopathologic features, prognosis is not different from IDC and determined by already known prognostic factors such as tumor size, lymph node metastasis. Therefore, the patients with MC also need aggressive treatment like IDC.

scholarly journals Neuropilin-1 expression is associated with lymph node metastasis in breast cancer tissues

2018 ◽  
Vol Volume 10 ◽  
pp. 1969-1974 ◽  
Author(s):  
Mahnaz Seifi-Alan ◽  
Roshanak Shams ◽  
Mojgan Bandehpour ◽  
Reza Mirfakhraie ◽  
Soudeh Ghafouri-Fard
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Node Metastasis ◽  
Neuropilin 1 ◽  
Cancer Tissues

scholarly journals The Association between ANXA2 mutations and Clinical Pathological Features in Patients with Breast Cancer

2021 ◽  
Author(s):  
Jiayou Liu ◽  
Shaoli Xie ◽  
Linglong Mo ◽  
Lulan Pu ◽  
Mingfei Xu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Molecular Subtype ◽  
Clinical Stage ◽  
Missense Mutations ◽  
Early Screening ◽  
Tissue Samples ◽  
Node Metastasis ◽  
Cancer Tissues

Abstract Background Genetic mutations have been reported in many tumors. In this study, we aimed to examine whether ANXA2 mutations occur in breast cancer and to investigate their association with clinicopathological characteristics in patients. Materials and Methods We collected breast cancer and adjacent normal tissue samples from 112 patients, extracted total RNA, and performed PCR-SSCP and bidirectional Sanger sequencing. ANXA2 mutations were identified by NCBI BLAST (blastn and blastx), and their correlation with clinical data were analyzed. Results ANXA2 mutations were detected in breast cancer tissues (missense mutations 38.39%) at a higher incidence than in adjacent normal tissues (missense mutations 8.04%) and mainly located in domain and repeats 1. Moreover, mutations in breast cancer tissues were associated with clinical stages, molecular subtype, ER, PR, and lymph-node metastasis of patients. Within a mean follow-up time of 52.5 months, the 5-year OS of patients with missense mutations was lower than those without. Among those mutations, c.(350AG > GA), c.(375G > A), c.(487T > A) and c.(693G > A) were associated with younger patients. c.(350AG > GA) was related to higher clinical stage and lymph-node metastasis. c.(375G > A) was linked to HER2(-) cases, while c.(693G > A) tended to be TNBC cases. Conclusion ANXA2 missense mutations mainly occurred on domain and repeats 1 in breast cancer, which may be associated with pathogenesis, clinical stage, molecular subtyping, lymph node metastasis and 5-year OS of breast cancer. These mutations may contribute to the early screening, diagnosis, and targeted treatment of breast cancer.

scholarly journals Association of HIF-1α and NDRG2 expression with EMT in gastric cancer tissues

2019 ◽  
Vol 14 (1) ◽  
pp. 217-223
Author(s):  
Ren-Xiang Wang ◽  
Xia-Wan Ou ◽  
Ma-Fei Kang ◽  
Zu-Ping Zhou
Keyword(s):  
Gastric Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Epithelial Mesenchymal Transition ◽  
Hypoxia Inducible Factor ◽  
Mesenchymal Transition ◽  
Node Metastasis ◽  
Significant Difference ◽  
Cancer Tissues ◽  
E Cadherin

AbstractObjectiveThis study aims to investigate the differences in the expression of hypoxia-inducible factor-1α (HIF-1α), N-myc downstream-regulated gene 2 (NDRG2) and epithelial mesenchymal transition (EMT)-related proteins in normal gastric tissues, gastric cancer tissues and lymph node metastasis.MethodsImmunohistochemistry was used to detect the expression of HIF-1α, NDRG2, E-cadherin, Snail and Twist in normal gastric tissues, gastric cancer tissues and lymph node metastasis.ResultsIn normal gastric tissues, HIF-1α was not expressed, NDRG2 was highly expressed. There was a significant between the expression of NDRG2 and Snail, as well as of NDRG2 and Twist. In gastric cancer tissues, there was no statistically difference between the expression of HIF-1α and E-cadherin, NDRG2 and E-cadherin. However, there was a significant difference in expression between the expression of HIF-1α and Snail, HIF-1α and Twist, NDRG2 and Snail, and NDRG2 and Twist. In lymph node metastasis tissues, we show that HIF-1α was highly expressed, while NDRG2 was not, and the difference between the expression of HIF-1α and E-cadherin, HIF-1α and Snail, HIF-1α and Twist was not significant.ConclusionHIF-1α may promote EMT, possibly by inhibiting the expression of NDRG2.

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