scholarly journals Edible bird's nest impact on rats' uterine histomorphology, expressions of genes of growth factors and proliferating cell nuclear antigen, and oxidative stress level

2018 ◽  
Vol 11 (1) ◽  
pp. 71-79 ◽  
Author(s):  
Abdulla A. Albishtue ◽  
Nurhusien Yimer ◽  
Md Zuki A. Zakaria ◽  
Abd Wahid Haron ◽  
Rosnina Yusoff ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Growth Factors ◽  
Stress Level ◽  
Proliferating Cell Nuclear Antigen ◽  
Nuclear Antigen ◽  
Oxidative Stress Level ◽  
Edible Bird’S Nest ◽  
Proliferating Cell ◽  
And Oxidative Stress

Asprosin and Oxidative Stress Level in COVID-19 Patients

2022 ◽  
Vol 68 (01/2022) ◽  
Author(s):  
E. Seyhanli ◽  
Ismail Koyuncu ◽  
I. Yasak ◽  
H. Demir ◽  
Ebru Temiz
Keyword(s):  
Oxidative Stress ◽  
Stress Level ◽  
Oxidative Stress Level ◽  
And Oxidative Stress

scholarly journals Decorin inhibits glucose-induced lens epithelial cell apoptosis via suppressing p22phox-p38 MAPK signaling pathway

2019 ◽  
Author(s):  
Shanshan Du ◽  
Jingzhi Shao ◽  
Dandan Xie ◽  
Fengyan Zhang
Keyword(s):  
Oxidative Stress ◽  
Epithelial Cell ◽  
P38 Mapk ◽  
Stress Level ◽  
High Glucose ◽  
Lens Epithelial Cell ◽  
Human Lens ◽  
Oxidative Stress Level ◽  
And Oxidative Stress ◽  
Cataract Patients

AbstractPurposeTo determine the effect of decorin on oxidative stress and apoptosis of human lens epithelial (HLE) cells under high glucose condition.MethodsHLE cell line (HLEB3) was incubated in normal glucose (5.5 mM) or high glucose (60 mM) medium. Decorin (50 nM) was applied 2 hours before high glucose medium was added. Apoptosis detection was executed by flow cytometry and western blotting (analysis of bcl-2 and bax). Oxidative stress level was measured by the generation of reactive oxygen species (ROS), glutathione peroxidase (GSH) and superoxide dismutase (SOD). P38 mitogen-activated protein kinase (MAPK) phosphorylation, the expression of p22phox of HLE cells and human lens anterior capsules were detected by western blotting. Small interfering RNA transfection to p22phox and p38 MAPK was also carried out on HLEB3.ResultsHigh glucose caused HLE cells oxidative stress and apoptosis exhibiting the increase of apoptotic cells and ROS production and decrease of bcl-2/bax ratio, GSH/GSSG ration and SOD activity. P22phox and phospho-p38 MAPK were upregulated in high glucose treated HLEB3 cells. Knocking down p22phox or p38 by siRNAs can reduce high glucose induced cell apoptosis and oxidative stress level. Silencing p22phox by siRNA can downregulate p38 MAPK activation. Decorin can inhibit the apoptosis, oxidative stress level and the induction of p22phox and p-p38 of HLEB3 induced by high glucose. Furthermore, the expression of p22phox and p38 were found significantly increased in lens anterior capsules of diabetic cataract patients compared to that of normal age-related cataract patients.ConclusionsResults showed that p22phox-p38 pathway may be particepated in high glucose induced lens epithelial cell injury, decorin may inhibit the high glucose induced apoptosis and oxidative stress injury by suppressing this pathway in part.

scholarly journals Melatonin Inhibits Apoptosis and Oxidative Stress of Mouse Leydig Cells via a SIRT1-Dependent Mechanism

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3084 ◽  
Author(s):  
Gaoqing Xu ◽  
Jing Zhao ◽  
Hongyu Liu ◽  
Jun Wang ◽  
Wenfa Lu
Keyword(s):  
Oxidative Stress ◽  
Leydig Cells ◽  
B Cell Lymphoma ◽  
Nuclear Antigen ◽  
Protective Effects ◽  
Mrna And Protein Expression ◽  
Silent Information Regulator 1 ◽  
Proliferating Cell ◽  
And Oxidative Stress ◽  
Dependent Mechanism

The purpose of the present study is to examine the effects of melatonin on apoptosis and oxidative stress in mouse Leydig cells and to elucidate the mechanisms responsible for these effects. Our results indicated that 10 ng/mL of melatonin significantly promoted cell viability, the ratio of EdU-positive (5-Ethynyl-2′-deoxyuridine) cells, and increased the mRNA expression of proliferating cell nuclear antigen (PCNA), cyclin D1(CCND1), and cell division control protein 42 (CDC42) (p < 0.05). We also observed that melatonin inhibited apoptosis of mouse Leydig cells, accompanied with increased B-cell lymphoma-2 (BCL-2) and decreased BCL2 associated X (BAX) mRNA and protein expression. Moreover, addition of melatonin significantly decreased the reactive oxygen species (ROS) production and malondialdehyde (MDA) and 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels, while it increased superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) levels (p < 0.05). In addition, we also found that melatonin increased the expression of SIRT1 (Silent information regulator 1) (p < 0.05). To explore the role of SIRT1 signaling in melatonin-induced cells, mouse Leydig cells were pretreated with EX527, an inhibitor of SIRT1. The protective effects of melatonin on mouse Leydig cells were reversed by EX527, as shown by decreased cell proliferation and increased cell apoptosis and oxidative stress. In summary, our results demonstrated that melatonin inhibited apoptosis and oxidative stress of mouse Leydig cells through a SIRT1-dependent mechanism.

scholarly journals Blood lead level modifies the association between dietary antioxidants and oxidative stress in an urban adult population

2012 ◽  
Vol 109 (1) ◽  
pp. 148-154 ◽  
Author(s):  
Yun-Chul Hong ◽  
Se-Young Oh ◽  
Sung-Ok Kwon ◽  
Min-Seon Park ◽  
Ho Kim ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stress Level ◽  
Geometric Mean ◽  
Lead Level ◽  
Blood Lead ◽  
Oxidative Stress Marker ◽  
Dietary Intakes ◽  
Dietary Antioxidants ◽  
Oxidative Stress Level ◽  
And Oxidative Stress

Oxidative stress may be affected by lead exposure as well as antioxidants, yet little is known about the interaction between dietary antioxidants and blood lead levels (BLL) on oxidative stress level. We investigated the interaction between dietary antioxidants and BLL on oxidative stress level. As part of the Biomarker Monitoring for Environmental Health conducted in Seoul and Incheon, Korea, between April and December 2005, we analysed data from 683 adults (female = 47·4 %, mean age 51·4 (sd 8·4) years) who had complete measures on BLL, dietary intakes and oxidative stress marker (urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG)). Dietary intakes were assessed by a validated semi-quantitative FFQ, BLL was measured using atomic absorption spectrophotometry, and 8-OHdG by ELISA. Multivariate linear regression analyses were used to evaluate the influence of BLL on the association between dietary antioxidants and 8-OHdG. Geometric means of BLL and 8-OHdG concentrations were 4·1 (sd 1·5) μg/dl and 5·4 (sd 1·9) μg/g creatinine, respectively. Increases of vitamins C and E were significantly associated with the decrease of log10 8-OHdG in the adults from the lowest quartile of the BLL group ( ≤ 3·18 μg/dl, geometric mean = 2·36 μg/dl) than those of the highest quartile BLL group (>5·36 μg/dl, geometric mean = 6·78 μg/dl). Regarding antioxidant-related foods, vegetables excluding kimchi showed a higher inverse relationship with 8-OHdG in the lowest quartile BLL group than the highest group. These findings suggest a rationale for lowering the BLL and increasing the intake of dietary antioxidants in the urban population in Korea.

Sign in / Sign up

Export Citation Format

Share Document