scholarly journals The Evaluation of Substrates and Trichoderma sp. Isolates for Cellulase Production

2018 ◽  
Vol 20 (1) ◽  
pp. 42-48
Author(s):  
Eka Triwahyuni ◽  
Yosi Aristiawan ◽  
Novita Ariani ◽  
Haznan Abimanyu ◽  
Trisanti Anindyawati
Keyword(s):  
Rice Bran ◽  
Wheat Bran ◽  
Cellulase Activity ◽  
Extraction Process ◽  
Cellulase Production ◽  
Empty Fruit Bunches ◽  
Second Generation Bioethanol ◽  
Trichoderma Sp ◽  
Efficient Producer ◽  
Filter Paper Assay

AbstractAs higher interest was on the lignocellulose-based or second generation bioethanol production, the research was then more focused on the production of cellulase, especially on the domestic enzyme. Trichoderma sp. is considered as one of the most efficient producer of cellulase. This study was conducted to investigate the performance of Trichoderma sp. on a variety of substrates to produce cellulase. Three types of substrate variations and three types of Trichoderma sp. were used in this experiment. The substrate used were wheat bran, rice bran and oil palm empty fruit bunches (EFBs), whereas Trichoderma sp. isolates were encoded as T004, T051 and T063. Production of cellulase was made by solid fermentation for 7 days. The analysis of cellulase activity was done by National Renewable Energy Laboratory (NREL) method for filter paper assay. The results showed that the type of substrate affected the performance of Trichoderma sp. All types of fungus produced cellulase on wheat bran substrate with activity of 0.52 FPU /ml for T004, 0.23 FPU/ml for T051 and 0.27 FPU /ml for T063. With the rice bran substrate and EFBs, only T004 could produce cellulase and the enzyme activity analyzed were 0.08 FPU /ml and 0.008 FPU/ml respectively. Optimation of the buffer addition on enzyme extraction process produces the highest activity 0.85 FPU/mL for T004 with wheat bran substrate. Keywords: cellulase, EFBs, rice bran , Trichoderma sp. , wheat bran

scholarly journals ULTRAVIOLET MUTAGENESIS OF LOCAL ISOLATE Trichoderma sp. T065 FOR IMPROVING CELLULASES ACTIVITY (Mutagenesis Isolat Lokal Trichoderma sp. T065 menggunakan Ultraviolet untuk meningkatkan Aktivitas Selulase)

2016 ◽  
Vol 6 (01) ◽  
Author(s):  
Trisanti Anindyawati ◽  
Eddy Jusuf ◽  
Haznan Abimanyu
Keyword(s):  
Uv Light ◽  
Air Flow ◽  
Carbon Sources ◽  
Cellulase Activity ◽  
Growth Zone ◽  
Wild Type ◽  
Empty Fruit Bunches ◽  
Whatman Filter Paper ◽  
Trichoderma Sp ◽  
Laminar Air Flow

Mutagenesis of indigenous fungal isolates Trichoderma sp. T065 was achieved by UV light in a laminar air flow and UV crosslinker to increase cellulase activity. Thirty-four mutants were tested for their growth capacity in mineral agar with several carbon sources: Whatman filter paper no.1, 1% carboxymethylcellulose (CMC), 2% cellulose powder, 1% Avicel and 4% delignified oil palm empty fruit bunches (DOPEFB) with granule size of 200 mesh. Three mutants (UV-1.1, 1.2-UV and UV-1.3) showed bigger growth zone on cellulose substrate of 4% DOPEFB than that of wild type Trichoderma sp. T065. The highest cellulase activities were 0.65 FPU/mL and 0.57 FPU/mL from UV-1.1 and UV-1- 3, respectively higher than wild type that is equal to 0.038 FPU/mL.Keywords: Trichoderma sp. T065, mutations, UV light, carbon source, cellulase activityABSTRAKMutagenesis isolat lokal kapang Trichoderma sp. T065 dilakukan dengan sinar UV pada laminar air flow dan UV crosslinker untuk meningkatkan aktivitas selulase. Tiga puluh empat kapang mutan diuji kapasitas pertumbuhannya pada mineral agar dengan beberapa jenis sumber karbon yaitu kertas saring Whatman no.1, 1% carboxymethylcellulose (CMC), 2% serbuk selulosa, 1% avicel dan 4% tandan kosong sawit (TKS) dengan ukuran granula 200 mesh. Tiga mutan (UV-1.1, UV-1.2 dan UV- 1.3) mempunyai zona pertumbuhan yang lebih besar pada substrat selulosa dengan sumber karbon 4% TKS daripada isolat asli Trichoderma sp. T065. Aktivitas selulase tertinggi adalah 0,65 FPU/mL dan 0,57 FPU/mL berturut-turut dari mutan UV-1.1 dan UV-1.3 yang lebih tinggi dari isolat aslinya yaitu 0,038 FPU/mL.Kata kunci : Trichoderma sp. T065, mutasi, sinar UV, sumber karbon, aktivitas selulase  

Cellulase Production by Submerged Fermentation Using Biological Materials of Corncob Residue with Aspergillus Niger FC-1

2013 ◽  
Vol 648 ◽  
pp. 116-119 ◽  
Author(s):  
Sheng Yao Jiang ◽  
Hui Qin Shi ◽  
Ming Fu Gao ◽  
Yun Pan Liu ◽  
Xiao Min Fang ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Carbon Source ◽  
Wheat Bran ◽  
Culture Medium ◽  
Fermentation Broth ◽  
Cellulase Activity ◽  
Cellulase Production ◽  
Medium Components ◽  
Shake Flasks ◽  
Optimal Medium

In order to improve the cellulase-producing ability, the culture medium components and condition were optimized. The results showed that the cellulase activity in the fermentation broth remarkably increased by using 1% corncob residue combined with 1% wheat bran and 1% corncob as carbon source, taking 0.75% soybean meal and 0.3% KON3 as nitrogen source, and adding 0.3% CaCO3 to stabilize the pH to 5.0. The FPase activity in supernatant of A. niger FC-1 fermented with 50mL of the optimal medium in 250mL shake flasks at 30°C reached 101.6U/mL, which was 4.7 times of that before optimization.

scholarly journals Biocatalyst: Cellulase Production in Solid State Fermentation (SSF) Using Rice Bran as Substrate

2020 ◽  
Vol 11 (1) ◽  
pp. 7689-7699
Keyword(s):  
Rice Bran ◽  
Nitrogen Sources ◽  
Cellulase Production ◽  
Culture Conditions ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Glucose Carbon ◽  
Biological Transformation ◽  
Fermentation Period ◽  
Filter Paper Assay

The study was aimed to analyze the biological transformation of cellulose in rice bran by Aspergillus flavus SB04 in SSF for 28 days. The culture conditions such as pH, temperature, moisture content were optimized for the effective production of the enzyme in SSF. Effect of carbon and nitrogen sources on cellulase production was further estimated in SMF and were quantified for 24hrs intervals for 7 days Maximum cellulase production for rice bran was observed to be high in glucose (carbon source) and yeast extract (nitrogen source) at initial moisture 75ml, pH 6, temperature 33°C and fermentation period was 14th day that was optimized using response surface methodology. The enzyme production was analyzed individually by dinitrosalicylic acid (DNS) method, Lowry protein estimation, and filter paper assay. The lignocellulosic degradation was observed and confirmed by FTIR and SEM. The degradation of cellulose periodically increases after 7 days, which influences the yield of cellulase enzyme.

Dietary Wheat Bran Oil Is Equally as Effective as Rice Bran Oil in Reducing Plasma Cholesterol

2018 ◽  
Vol 66 (11) ◽  
pp. 2765-2774 ◽  
Author(s):  
Lin Lei ◽  
Jingnan Chen ◽  
Yuwei Liu ◽  
Lijun Wang ◽  
Guohua Zhao ◽  
...  
Keyword(s):  
Rice Bran ◽  
Wheat Bran ◽  
Rice Bran Oil ◽  
Plasma Cholesterol ◽  
Wheat Bran Oil

scholarly journals Wild type Bacillus subtilis treated with ethyl methyl sulphonate produced catabolite insensitive mutants with improved cellulase production

2021 ◽  
Author(s):  
Oladipo Olaniyi
Keyword(s):  
Bacillus Subtilis ◽  
Carbon Source ◽  
Enzyme Production ◽  
Cellulase Activity ◽  
Cellulase Production ◽  
Minimal Salt Medium ◽  
Salt Medium ◽  
Production Medium ◽  
Wild Type ◽  
Ethyl Methyl

Abstract The goal of this present investigation was to mutagenize Bacillus subtilis with Ethyl Methyl Sulphonate (EMS), screen the mutants for cellulase production and evaluate the influence of different glucose concentrations on their cellulase production potentials. The wild type B. subtilis was treated with 20, 40, 60 and 80 µl of EMS and the mutants generated were screened for cellulase production in minimal salt medium containing carboxylmethylcellulose (CMC) as the carbon source. Quantitatively, cellulase activity and protein contents were determined by dinitrosalicylic acid and Lowry methods respectively. Seven mutants were developed from each of the EMS concentration bringing the total to twenty-eight from all the concentrations. Approximately 14 and 57% of the mutants developed from 40 and 60µl of EMS had higher cellulase activities than the wild type, while none of the mutants developed from 20 and 80 µl of EMS had better activities than the wild type. The supplementation of 0.2, 0.5, 1.0 and 1.5% glucose in enzyme production medium caused approximately 100, 14, 29 and 14% cellulase repression respectively in the mutants developed from 60µl EMS. Mutants MSSS02 and MSSS05 were considered as catabolite insensitive mutants because their cellulase production were enhanced in comparison to wild type.

scholarly journals Response surface methodology-artificial neural network based optimization and strain improvement of cellulase production by Streptomyces sp.

2020 ◽  
Vol 36 (4) ◽  
Author(s):  
Ega Soujanya Lakshmi ◽  
Manda Rama Narasinga Rao ◽  
Muddada Sudhamani
Keyword(s):  
Neural Network ◽  
Artificial Neural Network ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Wild Strain ◽  
Cellulase Activity ◽  
Cellulase Production ◽  
Optimal Conditions ◽  
Zone Formation ◽  
Artificial Neural

ABSTRACT Thirty seven different colonies were isolated from decomposing logs of textile industries. From among these, a thermotolerant, grampositive, filamentous soil bacteria Streptomyces durhamensis vs15 was selected and screened for cellulase production. The strain showed clear zone formation on CMC agar plate after Gram’s iodine staining.  Streptomyces durhamensis vs15 was further confirmed for cellulase production by estimating the reducing sugars through dinitrosalicylic acid (DNS) method. The activity was enhanced by sequential mutagenesis using three mutagens of ultraviolet irradiation (UV), N methyl-N’-nitro-N-nitrosoguanidine (NTG) and Ethyl methane sulphonate (EMS). After mutagenesis, the cellulase activity of GC23 (mutant) was improved to 1.86 fold compared to the wild strain (vs15). Optimal conditions for the production of cellulase by the GC 23 strain were evaluated using Response Surface Methodology (RSM) and Artificial Neural Network (ANN). Effect of pH, temperature, duration of incubation, , and substrate concentration on cellulase production were evaluated. Optimal conditions for the production of cellulase enzyme using Carboxy Methyl Cellulase as a substrate are 55 oC of temperature, pH of 5.0 and incubation for 40 h. The cellulase activity of the mutant Streptomyces durhamensis GC23 was further optimised to 2 fold of the activity of the wild type by RSM and ANN.  

scholarly journals Properties and Characteristics of Compressed Biofuel Production from Empty Palm Fruit Bunches

2019 ◽  
Vol 120 ◽  
pp. 02001
Author(s):  
Tanuwat Larptansuphaphol ◽  
Penja Jitjumroonchokchai
Keyword(s):  
Palm Oil ◽  
Extraction Process ◽  
Biofuel Production ◽  
Black Rice ◽  
Palm Fruit ◽  
Mixture Ratio ◽  
Heating Value ◽  
Empty Fruit Bunches ◽  
Comparison Results ◽  
Composition Ratios

Thailand sits in the third place of the global rankings for crude palm oil production with an annual output of approximately 2 million tons/p.a., or 1.2% of the global output. Empty palm fruit bunches is one of residual from palm oil extraction process of Palm Oil industry. The objective of this research was to study properties and characteristics of biofuel made from empty fruit bunches and mixed with black rice hush with various composition ratios of empty palm fruit bunches and black rice husk as 100:0, 90:10, 80:20, 70:30, 60:40 and 50:50. Only the biofuels with a ratio of 100:0 and 90:10 could be formed to be compressed biofuels. The heating values of biofuels were analyzed by Gallenkamp Autobomb Calorimeter whereas other fuel properties and characteristics were tested and analyzed according to ASTM standards. The comparison results between biofuel from a mixture ratio 100:0 and 90:10 showed that the average of heating value, ash content, carbon content and combustion time (minute per one piece) were 4,672.46 and 4,199.33 cal/g, 21.73% and 29.30%, 12.07% and 16.28%, 92.33 and 63 minutes, respectively.

Assessment of Wetting Kinematics and Cooling Performance of Select Vegetable Oils and Mineral-Vegetable Oil Blend Quench Media

2015 ◽  
Vol 830-831 ◽  
pp. 160-163 ◽  
Author(s):  
K.M. Pranesh Rao ◽  
K. Narayan Prabhu
Keyword(s):  
Vegetable Oil ◽  
Vegetable Oils ◽  
Rice Bran ◽  
Canola Oil ◽  
Rice Bran Oil ◽  
Extraction Process ◽  
Mineral Oil ◽  
Heat Extraction ◽  
Cooling Performance ◽  
Oil Blend

Quench hardening is a process where an alloy is heated to solutionizing temperature and held for a definite period, and then rapidly cooled in a quenching medium. Selection of quenchant that can yield desired properties is essential as it governs heat extraction process during quenching. In the present work, the cooling performance of vegetable oil and mineral-vegetable oil blend quench media was assessed. The vegetable oils used in this work were olive oil, canola oil and rice bran oil. The mineral-vegetable oil blends were prepared by blending 10 and 20 vol. % of rice bran and canola oil in mineral oil. Inconel probe of 12.5mm diameter and 60mm height, instrumented with thermocouples were used to characterize quenchants. The probe was heated to 850°C and quenched in the oil medium. The cooling curves at different locations in the probe were used to study wetting kinematics. Inverse modelling technique was used to estimate spatially dependent metal-quenchant interfacial heat flux. It was found that the vegetable oils exhibited very short vapour blanket stage compared to mineral oil and blends. Faster wetting kinematics obtained with blends resulted in uniform heat transfer compared to that of mineral oil. The temperature distribution in the probe quenched in vegetable oils and blends was more uniform compared to that in mineral oil. It is expected that the parts quenched in vegetable oils and blends would lead to better hardness distribution compared to mineral oils.

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