Cellulase Production by Submerged Fermentation Using Biological Materials of Corncob Residue with Aspergillus Niger FC-1

2013 ◽  
Vol 648 ◽  
pp. 116-119 ◽  
Author(s):  
Sheng Yao Jiang ◽  
Hui Qin Shi ◽  
Ming Fu Gao ◽  
Yun Pan Liu ◽  
Xiao Min Fang ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Carbon Source ◽  
Wheat Bran ◽  
Culture Medium ◽  
Fermentation Broth ◽  
Cellulase Activity ◽  
Cellulase Production ◽  
Medium Components ◽  
Shake Flasks ◽  
Optimal Medium

In order to improve the cellulase-producing ability, the culture medium components and condition were optimized. The results showed that the cellulase activity in the fermentation broth remarkably increased by using 1% corncob residue combined with 1% wheat bran and 1% corncob as carbon source, taking 0.75% soybean meal and 0.3% KON3 as nitrogen source, and adding 0.3% CaCO3 to stabilize the pH to 5.0. The FPase activity in supernatant of A. niger FC-1 fermented with 50mL of the optimal medium in 250mL shake flasks at 30°C reached 101.6U/mL, which was 4.7 times of that before optimization.

scholarly journals Wild type Bacillus subtilis treated with ethyl methyl sulphonate produced catabolite insensitive mutants with improved cellulase production

2021 ◽  
Author(s):  
Oladipo Olaniyi
Keyword(s):  
Bacillus Subtilis ◽  
Carbon Source ◽  
Enzyme Production ◽  
Cellulase Activity ◽  
Cellulase Production ◽  
Minimal Salt Medium ◽  
Salt Medium ◽  
Production Medium ◽  
Wild Type ◽  
Ethyl Methyl

Abstract The goal of this present investigation was to mutagenize Bacillus subtilis with Ethyl Methyl Sulphonate (EMS), screen the mutants for cellulase production and evaluate the influence of different glucose concentrations on their cellulase production potentials. The wild type B. subtilis was treated with 20, 40, 60 and 80 µl of EMS and the mutants generated were screened for cellulase production in minimal salt medium containing carboxylmethylcellulose (CMC) as the carbon source. Quantitatively, cellulase activity and protein contents were determined by dinitrosalicylic acid and Lowry methods respectively. Seven mutants were developed from each of the EMS concentration bringing the total to twenty-eight from all the concentrations. Approximately 14 and 57% of the mutants developed from 40 and 60µl of EMS had higher cellulase activities than the wild type, while none of the mutants developed from 20 and 80 µl of EMS had better activities than the wild type. The supplementation of 0.2, 0.5, 1.0 and 1.5% glucose in enzyme production medium caused approximately 100, 14, 29 and 14% cellulase repression respectively in the mutants developed from 60µl EMS. Mutants MSSS02 and MSSS05 were considered as catabolite insensitive mutants because their cellulase production were enhanced in comparison to wild type.

scholarly journals Gold Bioleaching from Printed Circuit Boards of Mobile Phones by Aspergillus niger in a Culture without Agitation and with Glucose as a Carbon Source

Metals ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 521 ◽  
Author(s):  
Rosalba Argumedo-Delira ◽  
Mario J. Gómez-Martínez ◽  
Brenda Joan Soto
Keyword(s):  
Aspergillus Niger ◽  
Carbon Source ◽  
Culture Medium ◽  
Printed Circuit Boards ◽  
Biological Processes ◽  
Circuit Boards ◽  
Printed Circuit ◽  
Material Resources ◽  
Low Energy Consumption ◽  
Fungal Consortium

Hydrometallurgical and pyrometallurgical processes to recover gold (Au) from cell-phone printed circuit boards (PCBs) have the disadvantage of generating corrosive residues and consuming a large amount of energy. Therefore, it is necessary to look for biological processes that have low energy consumption and are friendly to the environment. Among the biological alternatives for the recovery of Au from PCB is the use of cyanogenic bacteria and filamentous fungi in cultures with agitation. Considering that it is important to explore the response of microorganisms in cultures without agitation to reduce energy expenditure in the recovery of metals from PCB, the present investigation evaluated the capacity of Aspergillus niger MXPE6 and a fungal consortium to induce Au bioleaching from PCB in a culture medium with glucose as a carbon source and without agitation (pH 4.5). The results indicate that the treatments with PCB inoculated with the fungal consortium showed a considerable decrease in pH (2.8) in comparison with the treatments inoculated with A. niger MXPE6 (4.0). The fungal consortium showed a significantly higher Au bioleaching (56%) than A. niger MXPE6 (17%). Finally, the use of fungal consortia grown without agitation could be an alternative to recover metals from PCB, saving energy and material resources.

scholarly journals Enhanced Cellulase Production by Talaromyces Amestolkiae CMIAT055 using Banana Pseudostem

2021 ◽  
Author(s):  
Genilton S Faheina ◽  
Kally A Sousa ◽  
Jerri E Zilli ◽  
Carlos Vergara ◽  
Gustavo A. Saavedra Pinto ◽  
...  
Keyword(s):  
Culture Medium ◽  
Wild Strain ◽  
Cellulase Activity ◽  
Statistical Design ◽  
Ph Control ◽  
Cellulase Production ◽  
Statistical Experimental Design ◽  
Natural Carbon ◽  
The Cost ◽  
Hydrolysis Of

Abstract Cellulases are a complex of enzymes necessary for the complete solubilization of cellulose in sugars, thus playing a key role in the natural carbon cycle through the hydrolysis of lignocellulosic structures. The aim of this study was to evaluate the increase in the capacity of Talaromyces amestolkiae CMIAT 055 to produce cellulases by optimizing the components of the culture medium containing banana pseudostem as an inducer, as well as in different agitation configurations in a bioreactor. Optimization was performed through statistical experimental design (Plackett-Burman and DCCR), a study of pH control in bioreactors, and a study of the agitation system by comparing impellers with different flow profiles in the liquid medium. For this purpose, a wild strain of Talaromyces amestolkiae CMIAT 055 was used. In the Plackett-Burman and DCCR statistical design, four components of the culture medium were significant and optimized for greater synthesis of FPase: banana pseudostem, CaCl2, KH2PO4, and urea. In bioreactors tests, these parameters were beneficial for greater enzyme activities: maintenance of pH at 5.0, use of Pitched blade impeller, and rotation speed at 300 rpm. Comparing the first test using banana pseudostem in an Erlenmeyer flask to the last fermentation process in bioreactors, it was observed that the total cellulase activity increased from 424.7 FPU/L to 2172.8 FPU/L. This fact showed that the strategies adopted in this study are a pertinent way to reduce the cost of enzyme production through the use of lignocellulosic materials.

scholarly journals Optimization of Cellulase Production by Aspergillus niger ITBCC L74 with Bagasse as Substrate using Response Surface Methodology

2018 ◽  
Vol 25 (3) ◽  
pp. 115
Author(s):  
Abdullah Abdullah ◽  
Hamid Hamid ◽  
Marcelinus Christwardana ◽  
H. Hadiyanto
Keyword(s):  
Response Surface Methodology ◽  
Aspergillus Niger ◽  
Response Surface ◽  
Cellulase Activity ◽  
Cellulase Production ◽  
Lignocellulosic Material ◽  
Huge Amount ◽  
Activity Effect ◽  
Mgcl2 Concentration ◽  
Important Enzyme

Cellulase is a very important enzyme for lignocelluloses based ethanol production. Bagasse contains mainly cellulose (57.76%), hemicellulose (12.44%), lignin (21.34%), and others (7.96%). Lignocellulosic material has been considered as the good option for cellulase production because it is cheap and already available in a huge amount. The objective of this research was to produce cellulase enzyme and to optimize it by using response surface methodology. The bagasse with water content of 80% was incubated with 2 ml inoculum of Aspergillus niger ITBCC L74 in a 250 ml Erlenmeyer flask. After reaching the specified time the enzyme was extracted and then determined for its activity. Effect of process parameters such as pH, urea and MgCl2 addition were studied. The optimal cellulase activity was achieved at urea concentration of 4.5% (w/w), MgCl2 concentration of 1 mM and pH of 3.5, with maximum enzyme activity was 0.630 U/gr.

Optimization of Cellulase Production Using Agricultural Wastes by Artificial Neural Network and Genetic Algorithm

2011 ◽  
Vol 6 (1) ◽  
Author(s):  
Chun Chang ◽  
Guizhuan Xu ◽  
Junfang Yang ◽  
Duo Wang
Keyword(s):  
Neural Network ◽  
Genetic Algorithm ◽  
Artificial Neural Network ◽  
Solid State ◽  
Aspergillus Niger ◽  
Moisture Content ◽  
Trichoderma Viride ◽  
Cellulase Activity ◽  
Cellulase Production ◽  
Fermentation Time

The cellulase production by Trichoderma viride was optimized using artificial intelligence-based techniques under solid state fermentation. In this study, a back propagation network was designed with Levenberg-Marquardt training algorithm, and the tangent sigmoid and pure linear functions were used as the transfer functions in the hidden and output layers of the ANN, respectively. An artificial neural network coupling genetic algorithms was used to optimize the process parameters, which include the mass ratio of wheat straw to wheat bran, moisture content and fermentation time. The ultimate process parameters of optimization were mass ration of wheat straw to wheat bran 2.9, moisture content 69.6 percent, and fermentation time 123.3h. Further test experiment showed that the final cellulase activity can reach to 11.62 U/g, which was the highest value among all the experimental results. This result indicates that the genetic algorithm based on a neural network model is a better optimization method for cellulase production in solid state fermentation. To improve the cellulase production, a mixed culture system of Trichoderma viride and Aspergillus niger was also developed. The cellulase activity increased by 7.40 percent with the addition of Aspergillus niger at 72h.

scholarly journals Production of cellulase from Aspergillus niger and Trichoderma reesei mixed culture in carboxymethylcellulose medium as sole carbon

2019 ◽  
Vol 20 (12) ◽  
Author(s):  
Dia Septiani ◽  
HERMAN SURYADI ◽  
Abdul Mun'im ◽  
WIBOWO MANGUNWARDOYO
Keyword(s):  
Aspergillus Niger ◽  
Ammonium Sulfate ◽  
Mixed Culture ◽  
Trichoderma Reesei ◽  
Crude Extract ◽  
Hydrolytic Enzymes ◽  
Cellulase Activity ◽  
Cellulase Production ◽  
Partial Purification ◽  
Cellulose Depolymerization

Abstract. Septiani D, Suryadi H, Mun’im A, Mangunwardoyo W. 2019. Production of cellulase from Aspergillus niger and Trichoderma reesei mixed culture in carboxymethylcellulose medium as sole carbon. Biodiversitas 20: 3539-3544. Cellulase is one of hydrolytic enzymes that breakdown cellulose into glucose. Cellulases are promising to be applied in natural products which may improve the yield of bioactive in plant extract through cellulose depolymerization. Cellulases from mixed culture of Aspergillus niger and Trichoderma reesei can produce a high cellulase activity because of the synergism activity among endoglucanase, exoglucanase, and also β-glucoside. Cellulase production and partial purification of monoculture and mixed culture (1:1) of these fungi on carboxymethylcellulose media were investigated in this study. Total cellulase activity was measured by filter paper assay followed by protein estimation with Bradford method. The crude extract of Aspergillus niger monoculture has the highest cellulase activity (0.131 U/mL, P<005) followed by mixed culture (0.109 U/mL) and Trichoderma reesei (0.106 U/mL). The cellulase activity of partially purified cellulase from mixed culture significantly increased (0.335, 0.348, 0.374 U/mL, P<0.05) compared to crude extract along with stepwise addition of ammonium sulfate. Cellulase activity of mixed culture at 80% ammonium sulfate increase up to 2.238-fold and showed highest value (P<0.05) compared to monocultures. In conclusion, combination of Aspergillus niger and Trichoderma reesei fungi in carboxymethyl cellulose media followed by 80% ammonium sulfate precipitation can be a promising cellulase production with high cellulase activity.

scholarly journals Probing cellulolytic yeast from forest ecosystem for the saccharification of Napier fodder biomass

2021 ◽  
Vol 42 (4(SI)) ◽  
pp. 1152-1161
Author(s):  
M.G. Valliammai ◽  
◽  
N.O. Gopal ◽  
R. Anandham ◽  
◽  
...  
Keyword(s):  
Carbon Source ◽  
Statistical Model ◽  
Forest Ecosystem ◽  
Cellulase Activity ◽  
Cellulase Production ◽  
Media Composition ◽  
Yeast Isolate ◽  
Trichosporon Asahii ◽  
Fuels And Chemicals ◽  
The Media

Aim: This study aimed to search for novel cellulolytic isolates with high cellulase titre for the production of fuels and chemicals. Methodology: The yeast isolate YES5 isolated from the forest soil was screened for cellulase production. The cellulase activity of YES5 was optimized via RSM. The saccharification potential of YES5 using Napier biomass as substrate was evalauted. Results: The maximum cellulase activity obtained after optimizing pH, temperature, and incubation period was 35.70 U. A reliable statistical model was developed for maximizing the cellulase activity in YES5 Trichosporon asahii. The cellulase activity was 23.87U, when carbon source in CMC medium was replaced by Napier biomass. The maximum saccharification potential of 33.15% was observed on 3rd day. Interpretation: The study of optimizing the media composition of Trichosporon asahii cellulase using Napier biomass, a natural source of carbon for maximizing the cellulase production via RSM, is first of its kind.

scholarly journals The Evaluation of Substrates and Trichoderma sp. Isolates for Cellulase Production

2018 ◽  
Vol 20 (1) ◽  
pp. 42-48
Author(s):  
Eka Triwahyuni ◽  
Yosi Aristiawan ◽  
Novita Ariani ◽  
Haznan Abimanyu ◽  
Trisanti Anindyawati
Keyword(s):  
Rice Bran ◽  
Wheat Bran ◽  
Cellulase Activity ◽  
Extraction Process ◽  
Cellulase Production ◽  
Empty Fruit Bunches ◽  
Second Generation Bioethanol ◽  
Trichoderma Sp ◽  
Efficient Producer ◽  
Filter Paper Assay

AbstractAs higher interest was on the lignocellulose-based or second generation bioethanol production, the research was then more focused on the production of cellulase, especially on the domestic enzyme. Trichoderma sp. is considered as one of the most efficient producer of cellulase. This study was conducted to investigate the performance of Trichoderma sp. on a variety of substrates to produce cellulase. Three types of substrate variations and three types of Trichoderma sp. were used in this experiment. The substrate used were wheat bran, rice bran and oil palm empty fruit bunches (EFBs), whereas Trichoderma sp. isolates were encoded as T004, T051 and T063. Production of cellulase was made by solid fermentation for 7 days. The analysis of cellulase activity was done by National Renewable Energy Laboratory (NREL) method for filter paper assay. The results showed that the type of substrate affected the performance of Trichoderma sp. All types of fungus produced cellulase on wheat bran substrate with activity of 0.52 FPU /ml for T004, 0.23 FPU/ml for T051 and 0.27 FPU /ml for T063. With the rice bran substrate and EFBs, only T004 could produce cellulase and the enzyme activity analyzed were 0.08 FPU /ml and 0.008 FPU/ml respectively. Optimation of the buffer addition on enzyme extraction process produces the highest activity 0.85 FPU/mL for T004 with wheat bran substrate. Keywords: cellulase, EFBs, rice bran , Trichoderma sp. , wheat bran

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