in vivo Study of Central Nervous System Depressant Effects and Muscle Relaxant Activity of Methanolic Extract of Galphimia gracilis Leaf on Swiss Albino Mice

The investigation had explored the in vivo central nervous system (CNS) effects of novel isolated phytoconstituent from Galphimia glauca stems. The isolated molecule coded as “BS-2” was subjected to toxicological, central analgesic and central nervous system depressant studies on Swiss albino mice and Wistar rats. The LD-50 of BS-2 was found to be > 2000 mg/kg. The mice treated with BS-2 at 12.5, 25 and 50 mg/kg doses exhibited significant effect in the hot plate test (P < 0.5) and tail clip test (83 % inhibition). Mice treated with BS-2 prolonged the time of sleep induced by sodium pentobarbital (40 mg/kg, i.p.). Throughout the hole-board experiment and open field test the BS-2 at 50 mg/kg exhibited a significant (P ≤ 0.001) dose-dependent activity. The BS-2 was characterized by Rf value, melting point, IR spectra, mass spectra and 1H NMR spectrum. The BS-2 showed potential in vivo central pain relieving and central nervous system depressant effects.

Download Full-text

Long-term exposure to a butter-rich diet induces mild-to-moderate steatosis in Chang liver cells and Swiss albino mice models

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2016-0058 ◽

2017 ◽

Vol 28 (3) ◽

Author(s):

Thomas John Philip Nalloor ◽

Nitesh Kumar ◽

Kasinathan Narayanan ◽

Vasanth Raj Palanimuthu

Keyword(s):

Colorimetric Assay ◽

Liver Cells ◽

In Vivo Study ◽

Alcoholic Fatty Liver ◽

Swiss Albino Mice ◽

Special Diet ◽

Albino Mice ◽

Chang Liver

AbstractBackground:Butter is one of the widely used fats present in the diet. However, there is no satisfactory study available that evaluates the effect of a high-fat diet containing butter as the principal fat on the development of non-alcoholic fatty liver disease (NAFLD).Methods:In the present study, butter was used for the development of steatosis in Chang liver cells in an in vitro study and Swiss albino mice in an in vivo study. In vitro steatosis was established, and butter was compared with oleic acid in Chang liver cells using an oil red O (ORO)-based colorimetric assay. In the in vivo study, a butter-rich special diet was fed for 15 weeks to mice, who showed no significant change in body weight. The expression pattern of phosphatase and tensin homolog (Results and Conclusions:Special diet-fed animals showed downregulated

Download Full-text

In vivo analgesic potential in swiss albino mice and in vitro thrombolytic and membrane stabilizing activity of methanolic extract from suaeda maritima whole plant

Bagcilar Medical Bulletin ◽

10.5350/bmb20170306094829 ◽

2017 ◽

pp. 13-18 ◽

Cited By ~ 1

Author(s):

Mamunur Rashid ◽

Saddam Hussain ◽

Mamon Or Rashid ◽

Abdul Halim ◽

Niloy Sen ◽

...

Keyword(s):

Methanolic Extract ◽

Swiss Albino Mice ◽

Suaeda Maritima ◽

Whole Plant ◽

Albino Mice

Download Full-text

Assessment of Neuropharmacological Profile of Ethanolic Extract of Lawsonia Inermis Flowers

Mapana Journal of Sciences ◽

10.12723/mjs.55.4 ◽

2020 ◽

Vol 19 (4) ◽

Author(s):

Syeda Nishat Fathima Nishat Fathima ◽

Hemalatha G ◽

Smitha T ◽

Rama M

Keyword(s):

Central Nervous System ◽

Nervous System ◽

Muscle Relaxation ◽

Ethanolic Extract ◽

Maximal Electroshock ◽

Lawsonia Inermis ◽

Pharmacological Activities ◽

Leg Extension ◽

Central Nervous System Depressant ◽

Muscle Relaxant Activity

On preliminary basis neuropharmacological profile of ethanolic extract of flowers of Lawsonia inermis was carried out. For assessing the activity of flowers on central nervous system locomotor activity using actophotometer, muscle relaxant activity using rotarod apparatus, pentobarbital-induced hypnosis and anticonvulsant activity by maximal electroshock test was performed. Ethanolic extract of flowers of Lawsonia inermis decreased the motor activity by 48.63 % and showed 49.03% noteworthy muscle relaxation along with 127.73% potentiation of pentobarbital-induced sleeping time and 66.67% decreased the duration of tonic hind leg extension of seizures activity. The results conclude that the extract of flowers of Lawsonia inermis has significant central nervous system depressant activity. Further investigations are, however, necessary to explore mechanism(s) of action involved in these pharmacological activities.

Download Full-text

Evaluation of Antioxidant and Antinociceptive Properties of Methanolic Extract of Clerodendrum viscosum Vent.

Stamford Journal of Pharmaceutical Sciences ◽

10.3329/sjps.v4i1.8873 ◽

1970 ◽

Vol 4 (1) ◽

pp. 74-78 ◽

Cited By ~ 4

Author(s):

Md Mostafizur Rahman ◽

Nowshin N Rumzhum ◽

Kudrat-E-Khuda Zinna

Keyword(s):

Ascorbic Acid ◽

Methanolic Extract ◽

Antinociceptive Effect ◽

Reducing Power ◽

Antioxidant Property ◽

Swiss Albino Mice ◽

Albino Mice ◽

Clerodendrum Viscosum

The PDF for this article was updated on 18/11/2011.NN Rumzhum and K Zinna were added as authors on 18/11/2011.The methanolic extract of Clerodendrum viscosum vent. (Verbenaceae) was evaluated for in vitro antioxidant activity by determination of total antioxidant capacity, assay of nitric oxide scavenging activity and reducing power test and in vivo antinociceptive effect in acetic acid induced writhing model in swiss albino mice. The results revealed the presence of pronounced antioxidant property as compared with ascorbic acid used as standard and a dose-dependent (250 and 500 mg/kg) analgesic effect in Clerodendrum viscosum vent. The antioxidant and antinociceptive activities obtained seem to be in good accordance with the traditional uses of Clerodendrum viscosum. Key words: Clerodendrum viscosum; Verbenaceae; Antioxidant; Ascorbic acid; Antinociceptive; Swiss albino mice DOI: http://dx.doi.org/10.3329/sjps.v4i1.8873 SJPS 2011; 4(1): 74-78

Download Full-text