scholarly journals Synthesis of Horseradish Peroxidase-Gold Nanoparticle Conjugate through Green Route

2020 ◽  
Vol 32 (5) ◽  
pp. 1243-1247
Author(s):  
Lovnish Siyal ◽  
Benu Kumar ◽  
Ranjit Kumar ◽  
Rachana Sahney
Keyword(s):  
Gold Nanoparticles ◽  
Enzyme Activity ◽  
Horseradish Peroxidase ◽  
Gold Nanoparticle ◽  
Catalytic Efficiency ◽  
Hydrodynamic Diameter ◽  
Green Route ◽  
Peroxidase Enzyme ◽  
Uv Visible ◽  
Average Hydrodynamic Diameter

Synthesis of horseradish peroxidase-gold nanoparticle conjugates (HRP-AuNPs) has been studied for the development of biofunctionalized gold nanoparticles (AuNPs) through biogenic route. Herein, horseradish peroxidase enzyme has been used to synthesize gold nanoparticles at room temperature in tricine buffer. The morphology and size distribution of HRP-AuNPs conjugates were obtained by different techniques including dynamic light scattering (DLS), UV-visible (UV-vis) spectrophotometry, scanning electron microscopy (SEM) and energy dispersive X-ray analysis (EDX). The enzyme activity of HRP-AuNP conjugate was compared with free enzyme to determine their catalytic efficiency. The results suggests that HRP-AuNP conjugates are monodisperse particles with average hydrodynamic diameter of 83.93 ± 2.1 nm, zeta potential of about -18.4 ± 1.1 mV and higher enzyme activity towards H2O2 as compared to free horseradish peroxidase. These biofunctionalized gold nanoparticles could act as tag or labeling agent for various applications.

scholarly journals A simple and sensitive sensor for silver ions based on unmodified gold nanoparticles by using dynamic light scattering techniques

2017 ◽  
Vol 95 (12) ◽  
pp. 1267-1272 ◽  
Author(s):  
Zhiyou Xiao ◽  
Anjiang Tang ◽  
Hongsheng Huang ◽  
Ze Wang
Keyword(s):  
Gold Nanoparticles ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
High Sensitivity ◽  
Silver Ions ◽  
Hydrodynamic Diameter ◽  
Optimum Conditions ◽  
Sensitivity And Selectivity ◽  
Sensitive Sensor ◽  
Average Hydrodynamic Diameter

A simple and sensitive assay for Ag+ was developed with unmodified gold nanoparticles (AuNPs) by using dynamic light scattering techniques. Ag+ could induce the oligonucleotide (5′-ATC ACT ATA TCA TAT ACT CAT-3′) to change from a single-stranded structure to a double-stranded structure and desorb from the surface of AuNPs, which triggered the aggregation of AuNPs in the salt solution. The average hydrodynamic diameter of aggregated AuNPs could be detected by using dynamic light scattering techniques. Under the optimum conditions, the average hydrodynamic diameter of AuNPs is proportional to the concentration of Ag+ within the range of 13.3–100.0 nmol/L, with a detection limit of 3.2 nmol/L. The method is easy to operate and has low sample consumption, high sensitivity and selectivity.

The Synthesis and Characterization of PEG-SH-Modified Gold Nanoparticle in One-Pot Synthesis by Stenotrophomonas maltophilia

2019 ◽  
Vol 19 (11) ◽  
pp. 7278-7284
Author(s):  
Jian-Yuan Huang ◽  
Min-Hua Chen ◽  
Feng-Huei Lin
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Stenotrophomonas Maltophilia ◽  
Culture Medium ◽  
Synthesis And Characterization ◽  
One Pot ◽  
Residual Solvent ◽  
Green Route ◽  
The Relationship

Colloidal gold nanoparticles have been researched and utilized in many technical applications. However, the conventionalmethods to produce polyethyleneglycol (PEG) immobilized gold nanoparticles have to take several steps, including residual solvent removing. In the study, we propose an idea green route to synthesize gold nanoparticles by using Stenotrophomonas maltophilia in a one-pot reaction. The relationship between Au precursor and S. maltophilia was evaluated systematically. After PEG-SH addition, the bacterial cell wall was broken down and the synthetic nanoparticles could be released into culture medium. Furthermore, we identified that the crystal structure of synthetic gold nanoparticle was face-center cubic and PEG-SH was immobilized on synthetic gold nanoparticle ideally. The size of Au-PEG-SH was smaller than 30 nm. These findings suggest that gold nanoparticle with PEG-SH modification could be prepared in an eco-friendly one-pot reaction through the metabolic activity of S. maltophilia.

scholarly journals Optical Nonlinear Refractive Index of Laser-Ablated Gold Nanoparticles Graphene Oxide Composite

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Amir Reza Sadrolhosseini ◽  
A. S. M. Noor ◽  
Nastaran Faraji ◽  
Alireza Kharazmi ◽  
Mohd. Adzir Mahdi
Keyword(s):  
Particle Size ◽  
Gold Nanoparticles ◽  
Refractive Index ◽  
Graphene Oxide ◽  
Gold Nanoparticle ◽  
Spherical Shape ◽  
Refractive Indices ◽  
Hydroxyl Groups ◽  
Uv Visible ◽  
Laser Ablation Technique

Gold nanoparticles were prepared in graphene oxide using laser ablation technique. The ablation times were varied from 10 to 40 minutes, and the particle size was decreased from 16.55 nm to 5.18 nm in spherical shape. The nanoparticles were capped with carboxyl and the hydroxyl groups were obtained from Fourier transform infrared spectroscopy. Furthermore, the UV-visible peak shifted with decreasing of nanoparticles size, appearing from 528 nm to 510 nm. The Z-scan technique was used to measure the nonlinear refractive indices of graphene oxide with different concentrations and a gold nanoparticle graphene oxide nanocomposite. Consequently, the optical nonlinear refractive indices of graphene oxide and gold nanoparticle graphene oxide nanocomposite were shifted from1.63×10-9 cm2/W to4.1×10-9 cm2/W and from1.85×10-9 cm2/W to5.8×10-9 cm2/W, respectively.

Ternary covalent conjugate (antibody–gold nanoparticle–peroxidase) for signal enhancement in enzyme immunoassay

RSC Advances ◽  
2016 ◽  
Vol 6 (54) ◽  
pp. 48827-48833 ◽  
Author(s):  
M. M. Vdovenko ◽  
N. A. Byzova ◽  
A. V. Zherdev ◽  
B. B. Dzantiev ◽  
I. Yu. Sakharov
Keyword(s):  
Gold Nanoparticles ◽  
Horseradish Peroxidase ◽  
Enzyme Immunoassay ◽  
Gold Nanoparticle ◽  
Signal Enhancement ◽  
Enzyme Content ◽  
High Enzyme ◽  
Covalently Bound

A synthesis of a new horseradish peroxidase (HRP) conjugate and antibody covalently bound with gold nanoparticles (GNPs) with a high enzyme content was first developed.

Enzyme catalyzed polymerization and precipitation of aromatic compounds from aqueous solution

1993 ◽  
Vol 20 (5) ◽  
pp. 725-735 ◽  
Author(s):  
J. A. Nicell ◽  
J. K. Bewtra ◽  
N. Biswas ◽  
C. C. St. Pierre ◽  
K. E. Taylor
Keyword(s):  
Hydrogen Peroxide ◽  
Horseradish Peroxidase ◽  
Aromatic Compounds ◽  
Thermal Inactivation ◽  
Catalytic Efficiency ◽  
Reaction Products ◽  
Aromatic Substrate ◽  
Aromatic Substrates ◽  
Minimum Residual ◽  
Peroxidase Enzyme

Horseradish peroxidase enzyme (HRP), once activated by hydrogen peroxide, initiates the oxidation of a wide variety of aromatic compounds. Reaction products undergo a non-enzymatic polymerization to form water insoluble aggregates which are readily separated from solution. HRP was selected for application in wastewater treatment systems due to its stability and retention of its catalytic ability over wide ranges of pH and temperature. HRP activity was optimal between pH 5.7 and 8.5 with peak activity occurring at neutral pH. Activity increased with temperature up to 50 °C and declined at higher temperatures due to thermal inactivation. HRP was inactivated rapidly by hydrogen peroxide in the absence of an aromatic substrate. The efficiency of removal that was achieved was dependent on the nature of the aromatic undergoing treatment and the amount of enzyme provided due to the finite lifetime of the catalyst. Optimization of pH significantly improved catalytic efficiency with a corresponding savings in treatment costs. Optimal catalytic lifetime of HRP was achieved in the pH range of 7 to 9 for the eight phenolic compounds treated. The minimum residual level to which aromatic substrates were removed from solution was independent of the starting concentration of the aromatic substrate. Enhanced removal of hard-to-remove compounds was noted when mixtures of aromatic substrates were treated. Key words: horseradish peroxidase enzyme, polymerization, wastewater, phenols, aromatics amines.

scholarly journals Controllable preparation of highly active horseradish peroxidase-gold nanoparticle bionanoconjugate

2012 ◽  
Vol 14 (4) ◽  
pp. 57-60
Author(s):  
Peng Zhang ◽  
Chunli Liu ◽  
Shanshan Song ◽  
Chifang Peng
Keyword(s):  
Gold Nanoparticles ◽  
Enzyme Activity ◽  
High Activity ◽  
Immobilized Enzyme ◽  
Absorption Method ◽  
Horse Radish Peroxidase ◽  
Highly Active ◽  
Peroxidase Enzyme ◽  
Novel Method ◽  
Controllable Preparation

Abstract A novel method of immobilizing horse radish peroxidase enzyme (HRP) onto the surface of gold nanoparticles (GNPs) was developed. As a result, a high-activity bionanoconjugates was obtained through utilizing the biotin-streptavidin (SA) system. The HRP-SA-GNP bionanoconjugate with high activity was conveniently prepared through the biotin- avidin system. Compared with the HRP-GNP bioconjugate prepared through the traditional electrostatic absorption method, the enzyme activity per GNPs of this new bionanoconjugate was enhanced by 10 times. Moreover, the enzyme activity of this bionanoconjugate was controllable. The above method of bionanoconjugation preparation has promising applications in the fields including preparing highly active bio-nanoprobe and immobilized enzyme.

scholarly journals Evaluation of low concentration glyphosate effect on horseradish peroxidase enzyme activity

2020 ◽  
Vol 16 (1/2) ◽  
pp. 46
Author(s):  
Andrea Tansini ◽  
Valdir Eduardo Olivo ◽  
Jacir Dal Magro ◽  
Rafael Henrique Martello ◽  
Renan De Souza Rezende ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Horseradish Peroxidase ◽  
Low Concentration ◽  
Peroxidase Enzyme

scholarly journals Optical, Structural, and Antimicrobial Study of Gold nanoparticles Synthesized Using an Aqueous Extract of Mimusops elengi Raw Fruits

2020 ◽  
Vol 10 (6) ◽  
pp. 7085-7096
Keyword(s):  
Gold Nanoparticles ◽  
Aqueous Extract ◽  
X Ray Diffraction ◽  
X Ray ◽  
Green Route ◽  
Infra Red ◽  
Hazardous Condition ◽  
Uv Visible ◽  
Mimusops Elengi ◽  
Positive Results

Gold nanoparticles (AuNPs) has been synthesized via a green route using an aqueous extract of Mimusops elengi raw fruits. The phytochemicals of the fruit help in stabilizing and capping of the nanoparticles. The formation and stability of the synthesized samples has been explained based on results that we obtain from UV-Visible spectroscopy, Fourier Transform Infra-Red (FTIR) spectroscopy, Dynamic Light Scattering (DLS), X-ray diffraction (XRD), scanning electron microscopy (SEM). Further, the positive results of the antimicrobial test add one more novelty to this work. The axiom of this work is the synthesis of stable AuNPs without using any external stabilizing or reducing agents. The formation of AuNPs with the help of photochemical present in Mimusops elengi is yet another important aspect of this work, which provides an eco-friendly method for the synthesis of AuNPs, which can be used extensively because of its non-hazardous condition.

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