scholarly journals DETERMINATION of QUANTITATIVE CONTENT of EXTRACTIVE SUBSTANCES FROM the WOOD, ROOTS AND BARK of CONIFEROUS SPECIES in SIBERIA: LARCH (LARIX SIBIRICA L.), Scotch PINE (PINUS SYLVESTRIS L.), FIR (ABIES SIBIRICA L.), SPRUCE (PICEA OBOVATA L.) AND CEDAR (PINUS SIBIRICA DU TOUR.)

2018 ◽  
pp. 185-195 ◽  
Author(s):  
Людмила (Lyudmila) Андреевна (Andreyevna) Остроухова (Ostroukhova) ◽  
Татьяна (Tat'yana) Евгеньевна (Evgen'evna) Федорова (Fedorova) ◽  
Нина (Nina) Аркадьевна (Arkad'yevna) Онучина (Onuchina) ◽  
Алексей (Aleksey) Александрович (Aleksandrovich) Левчук (Levchuk) ◽  
Василий (Vasiliy) Анатольевич (Anatol'yevich) Бабкин (Babkin)
Keyword(s):  
Ethyl Acetate ◽  
Larix Sibirica ◽  
Water Soluble ◽  
Raw Material ◽  
Pinus Sibirica ◽  
Picea Obovata ◽  
Coniferous Species ◽  
Quantitative Content ◽  
Extractive Substances ◽  
Pinus Sibirica Du Tour

The study of the quantitative content of extractive substances from various tree organs (wood, roots, bark) of coniferous Siberian specieswas conducted. The larch (Larix sibirica L.), pines (Pinus sylvestris L.), fir (Abies sibirica L.), spruce (Picea obovata L.) and cedar (Pinus sibirica Du Tour) were investigated. Samples of coniferous tissues were selected in the Lake Baikal area in September-October 2015. Extractive substances were extracted from the ground raw material by extraction with ethyl acetate at the boiling point of the solvent for 4 hours. The resinous substances were extracted from the obtained ethyl acetate extracts by hexane by the infusion method for 2 days. The raw material samples, after extraction with ethyl acetate, were dried from solvent residues, then water-soluble substances were extracted by extraction with distilled water at 90 °C for 4 hours [1]. It is established the bark of the investigated coniferous species is most rich in extractive substances. It can contain up to 15% of the mass of absolutely dry matter (a.d.m.) of compounds soluble in ethyl acetate (for example, fir bark). The yield of extractive substances from coniferous wood is comparable with their content in the roots and ranges from 2 to 6% of the a.d.m. The greatest amount of resinous substances contain in wood of cedar and pine. This is more than 3% of a.d.m. The highest yield of water-soluble substances was from larch wood (18% of a.d.m.) and fir bark (10% of a.d.m.). A generalization and systematization of data on the chemical composition of phenolic extractive compounds of various coniferous tissues was carried out. It is shown that all the species under study contain a variety of biologically valuable polyphenols and have good potential for pharmacology, agriculture and forestry.

scholarly journals Sub-recent palynological spectra of landscapes of the Yamalo-Nenets Autonomous Area, Russia

2020 ◽  
Author(s):  
N.V. Shefer ◽  
◽  
L. S. Shumilovskikh ◽  
I.I. Gureyeva ◽  
◽  
...  
Keyword(s):  
Northern Taiga ◽  
Taxonomic Composition ◽  
Larix Sibirica ◽  
Pinus Sibirica ◽  
Picea Obovata ◽  
Forest Tundra ◽  
Pinus Sibirica Du Tour ◽  
Betula Pubescens Ehrh ◽  
Nenets Autonomous ◽  
Actual Participation

An analysis of the composition of surface spore-pollen spectra (SPS) was carried out within a gradient from forest-tundra to northern taiga. In total, 20 sub-recent moss-lichen surface samples were obtained on the territory of the Nadymsky and Purovsky districts of Yamalo-Nenets Autonomous Area. All studied spectra contain pollen of Larix sibirica Ledeb., Picea obovata Ledeb., Pinus sibirica Du Tour, P. sylvestris L., Betula pubescens Ehrh., B. nana L, as well as Ericaceae, Asteraceae and Cyperaceae. In the SPS of the studied communities, an increase in the participation of the pollen of Pinus species, a decrease in the content of Betula nana pollen, and an increase in the diversity of the taxonomic composition of herbs at the transition from the forest-tundra to the northern taiga are observed. The low content of Picea obovata pollen in the forest-tundra and northern taiga spectra reflects the low proportion of spruce in the studied communities. The low pollen proportion of Larix sibirica in the forest-tundra and northern taiga does not reflect the actual participation of L. sibirica in the vegetation cover, but is associated with rapid destruction of pollen and its low flight ability. The low content of Larix sibirica pollen in the forest-tundra and northern taiga does not reflect the actual participation of larch in the stand.

scholarly journals PHENOLIC COMPOUNDS OF PICEA OBOVATA LEDEB. BARK

2017 ◽  
pp. 89-95 ◽  
Author(s):  
Татьяна (Tat'iana) Евгеньевна (Evgen'evna) Федорова (Fedorova) ◽  
Сергей (Sergei) Владимирович (Vladimirovich) Федоров (Fedorov) ◽  
Василий (Vasilii) Анатольевич (Anatol'evich) Бабкин (Babkin)
Keyword(s):  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
13C Nmr Spectroscopy ◽  
Water Soluble ◽  
Picea Obovata ◽  
Siberian Spruce ◽  
Spruce Bark ◽  
Extractive Substances ◽  
Infusion Method

Chemical composition of a phenolic complex of the Siberian spruce bark (Picea obovata Ledeb.) and a structure their oligomeric phenolic compounds are studied.The samples of the spruce bark have been collected in the vicinity of Fr. Baikal in September 2015. Extractive substances were recovered from the crushed bark by extraction with ethyl acetate at the boiling point of the solvent for 4 hours. The resinous substances were extracted from the obtained ethyl acetate extracts by hexane by the infusion method for 3 days. The bark samples, after extraction with ethyl acetate, were dried to remove solvent residues, then the water-soluble substances were extracted by extraction with distilled water at 90 °C for 4 hours.The yields of the extractive substances extracted with ethyl acetate and water from the spruce bark were 3,4 and 6,9% in absolutely dry bark (a.d.b.), respectively. The content of phenolic compounds in the ethyl acetate extract of the spruce bark have been 42,4% relative. The ethyl acetate extract of a spruce bark, pretreated with hexane, was fractionated on a silica gel column using mixture chloroform-acetone as the eluent with an increase in the fraction of the latter (from 0 to 100%). The content of monomeric and oligo-, polymeric phenolic compounds in the extract was determined.Based on the data of 1Н and 13C NMR spectroscopy, it was established that the flavonoid dihydroquercetin and stilbene glycosides – isorhapontin and astringin are the main monomeric phenolic extractive substances of Siberian spruce bark. It is shown that the structure of oligomeric phenolic compounds of the spruce bark includes structural fragments of stilbene glycosides, mainly monomeric blocks with the structure of isorhapontin and astringin.

A study of natural durability of selected coniferous wood species from north Asia affected by the fungus Coniophora puteana (Schumach.) P. Karst.

2020 ◽  
Vol 112 ◽  
pp. 32-35
Author(s):  
Boguslaw Andres ◽  
Agnieszka Jankowska ◽  
Grzegorz Duchnik
Keyword(s):  
Wood Species ◽  
Brown Rot ◽  
Larix Sibirica ◽  
Natural Durability ◽  
Pinus Sibirica ◽  
European Larch ◽  
Coniophora Puteana ◽  
North Asia ◽  
Coniferous Wood ◽  
Pinus Sibirica Du Tour

The aim of prestened study was to determine the natural durability of selected coniferous wood species from Asia to the effect of Coniophora puteana (Schumach.) P. Karst., which causes brown rot. The species tested were: Larix gmellini (Rupr.) Kuzen., Larix sibirica Ledeb. and Pinus sibirica Du Tour.. The test was conducted on the basis of PN-EN 350:2016-10 and PN-EN 113:2000. As a result of the research durability classes were determined. Wood durability of tested Asian species was compared with the domestic wood species such as Pinus sylvestris L. and Larix decidua Mill.. The most durable of the tested wood species was Larix sibirica Ledeb., while the European larch showed comparable durability. The wood of Larix gmellini (Rupr.) Kuzen. showed the lowest persistence among the tested species.

scholarly journals Pharmacognostic analysis of Salvia hispanica L. seeds

2021 ◽  
pp. 49-54
Author(s):  
Anastasiia Sytryn ◽  
Iryna Cholak ◽  
Oksana Yemelianova ◽  
Uliana Karpiuk
Keyword(s):  
Fatty Acids ◽  
Chemical Reactions ◽  
Hydroxycinnamic Acids ◽  
Water Soluble ◽  
Raw Material ◽  
Salvia Hispanica ◽  
Phytochemical Study ◽  
Quantitative Content ◽  
Salvia Hispanica L ◽  
Material Swelling

The aim. The aim of this work was to conduct a microscopic and phytochemical study of the seeds of chia (Salvia hispanica L.). Materials and methods. Chia seeds were examined macroscopically and microscopically. To study the qualitative composition of the main groups of biologically active substances, histochemical, microchemical and chemical reactions were used. Hydroxycinnamic acids were identified by paper chromatography. To obtain a lipophilic extract, a Soxhlet apparatus and an exhaustive chloroform extraction method were used. The study of the quantitative content of fatty acids was carried out by gas chromatography. The content of polysaccharides in the raw material was determined by the gravimetric method. According to the SPhU method, the raw material swelling index was determined. Results. The main macro- and microscopic features of chia seeds have been established. Histochemical reactions, microchemical reactions made it possible to establish the presence of mucus and fatty oils in chia seeds. With the help of chemical reactions, the presence of flavonoids in the raw material was established. The quantitative content of fatty oils is 24.0±1.2 %. The content of water-soluble polysaccharides in the whole raw material was 4.01±0.07 %, in the crushed raw material - 5.04 ± 0.05 %. As a result of determining the swelling index, it was found that this indicator for the whole chia seeds was 20, and for the crushed ones – 17. The content of hydroxycinnamic acids in the chia seeds was 1.07±0.03 %. 9 fatty acids have been identified, among which linoleic acid predominates in terms of content. Conclusions. The presence and quantitative content of mucus, fatty oils, water-soluble polysaccharides, flavonoids, hydroxycinnamic acids, fatty acids was confirmed in the seeds of chia (Salvia hispanica L.). The obtained data can be used to develop regulatory documentation for chia seeds in order to use this raw material in pharmacy and medicine

scholarly journals Geoactive Zones and the Structure of Plant Communities in Connection With New Discoveries in Space Geology

2021 ◽  
Vol 7 (1) ◽  
pp. 33-48
Author(s):  
M. Rogozin
Keyword(s):  
Pinus Sibirica ◽  
Comfort Zone ◽  
Space Images ◽  
Picea Obovata ◽  
Siberian Spruce ◽  
Large Trees ◽  
Ring Structures ◽  
Pinus Sibirica Du Tour ◽  
Siberian Pine ◽  
Geodynamic Activity

The purpose of the work is to find out how plants react to such zones and whether their phytoindication is possible. Two territories of the Perm territory were studied: the Vishersky Nature Reserve and forests near Perm. The research methodology included lineament and geostructurometric analysis of space images and special maps using ring structures discovered on the Earth’s surface by Yu. I. Fivenskii, as well as phyto- and bioindication of small geoactive zones. It was found that in the forests near Perm favorable zones of such zones occupy 1.44% of the territory and within their limits the safety of common pine (Pinus sylvestris L.) is 29–42 times higher. Phytoindication of small geoactive zones of 1.0 and 3.0 m by common pine was shown in the zones of influence of these zones: 0–18 cm — the radius of inhibition with the absence of trees; 19–29 cm — the depression zone with small trees; 30–48 cm — the comfort zone with medium and large trees. Two types of networks formed by zones of 1.0 and 8.0 m in size near Perm (on the plain) and in the reserve (in the mountains) were compared. In the mountains, with high geodynamic activity of territories, the networks are oriented in one direction, while on the plain their orientation is misaligned by 30°. In the reserve, large trees of Siberian spruce (Picea obovata Ledeb.) were located on small geoactive zones of 1.0 and 8.0 m, and trees of Siberian pine (Pinus sibirica Du Tour) — on zones 1.0 and 3.0 m in combination with zones 16, 32 and 55 m. In the subgolets zone, ring structures of perennial plants were also found on the first two types of zones. In addition, along the edges of the swamps, chains of trees were found whose direction coincides with ring faults, and the chains themselves are similar to the structure of small geoactive zones networks, and this fractality requires field verification. The hypothesis recharge of plants within geoactive zones is proposed, which also explains the successful growth of trees on rocks, where the amount of available soil is minimal.

FUNGI COLONIZING CONIFEROUS SEEDLINGS IN FOREST NURSERIES OF MIDDLE SIBERIA

2021 ◽  
Vol 14(63) (1) ◽  
pp. 57-66
Author(s):  
Marina A. SHELLER ◽  
Elena A. SHILKINA ◽  
Aleksey A. IBE ◽  
Tatyana V. SUKHIKH ◽  
Inna E. SAFRONOVA
Keyword(s):  
Pinus Sibirica ◽  
Stone Pine ◽  
Siberian Stone Pine ◽  
Picea Obovata ◽  
Siberian Spruce ◽  
Phialocephala Fortinii ◽  
Phytosanitary Measures ◽  
Forest Nurseries ◽  
Pinus Sibirica Du Tour ◽  
Middle Siberia

The study was carried out in four forest nurseries of Middle Siberia. Affected seedlings of Scots pine (Pinus sylvestris L.), Scots Siberian stone pine (Pinus sibirica Du Tour), and Siberian spruce (Picea obovata Ledeb.) were collected for molecular phytopathological examination. In total, 14 fungal taxa were identified in the needles and roots of the plants. The most dominant among them were Sydowia polyspora (Bref. & Tavel) E., Didymella glomerata (Corda) Qian Chen & L. Cai, Cladosporium herbarum (Pers.) Link, Lophodermium seditiosum Minter, Staley & Millar, Phialocephala fortinii C. J. K. Wang & H. E. Wilcox and Cadophora finlandica (C. J. K. Wang & H. E. Wilcox) T. C. Harr. & McNew. The richness of the fungal taxa was higher in the needles than in the roots of all the tree species studied. The obtained results could be used for implementing more effective phytosanitary measures in the studied nurseries.

scholarly journals CONTENT OF EXTRACTIVE SUBSTANCES AND POLYSACCHARIDES IN FRUIT BODIES OF GRIFOLA FRON-DOSA CULTIVATED ON LIGNOCELLULUS SUBSTRATES DEPENDING ON EXTRACTION METHODS

2020 ◽  
pp. 241-247
Author(s):  
Denis Viktorovich Minakov ◽  
Yuriy Vasil'yevich Morozhenko ◽  
Natal'ya Grigor'yevna Bazarnova
Keyword(s):  
Comparative Analysis ◽  
Extraction Method ◽  
Extraction Methods ◽  
Water Soluble ◽  
Glass Containers ◽  
Quantitative Content ◽  
Soxhlet Apparatus ◽  
Monomeric Composition ◽  
Extractive Substances ◽  
Hydrolysis Of

The work is devoted to a comparative analysis of the quantitative content of extractives and water-soluble polysaccharides isolated from the fruit bodies of mushrooms Grifola frondosa depending on the extraction methods. As objects of study used strain of fungi G. frondosa 2639, isolated from commercial mycelium. It was established that the yield of fungi in polypropylene bags (volume up to 4500 cm3) was 36.37%, differing little from the yield of fruit bodies in glass containers (volume 800–1000 cm3). Using the hydrolysis of extraction products it was found that the methods of extracting the fruit bodies of G. frondosa with water lead to the extraction of polysaccharides of different composition. It was established that when using extraction methods in the Soxhlet apparatus, boiling and maceration (25.0±1.0 °C), the yield of extractive substances from the fruit bodies of G. frondosa was within the literature data interval and amounted to 6.04, 5.81 and 3.45%, respectively. It was shown that the highest content of water-soluble polysaccharides in the fruit bodies of G. frondosa was found using the extraction method in the Soxhlet apparatus – 14.92 %, differing from boiling and maceration methods (25.0±1.0 °C) in 1.15 and 1.33 times. By HPLC it was found that when maceration extraction was used (25.0±1.0 °C) and boiling, the monomeric composition of the polysaccharides of the fruit bodies of G. frondosa is mainly glucose, mannose, fucose and rhamnose in a ratio of 1.0 : 0.9 : 0.4 : 1.3, respectively. During extraction in the Soxhlet apparatus, their ratio was 1.0 : 1.2 : 0.5 : 1.9.

scholarly journals PHENOLIC COMPOUNDS OF CEDAR WOOD (SIBERIAN PINE) PINUS SIBIRICA DU TOUR.

2020 ◽  
pp. 97-104
Author(s):  
Tat'yana Yevgen'yevna Fedorova ◽  
Sergey Vladimirovich Fedorov ◽  
Vasily Anatolyevich Babkin
Keyword(s):  
Chemical Composition ◽  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Complex Mixture ◽  
13C Nmr Spectroscopy ◽  
Biologically Active ◽  
Pinus Sibirica ◽  
Pinus Sibirica Du Tour ◽  
Siberian Pine

As part of the study of extractives of biomass of coniferous trees of Siberia in order to predict the possibility of obtaining valuable biologically active products, a study was conducted of the chemical composition of the ethyl acetate extract of cedar wood (Siberian pine) Pinus sibirica Du Tour. The chemical composition of the phenolic compounds of cedar wood was studied, including the structure of oligomeric polyphenols. The yields of extractives extracted with ethyl acetate and water from cedar wood were 1.6 and 2.71% of a.s. (absolutely dry matter). The content of phenolic compounds in the ethyl acetate extract of cedar wood was 45.9% rel. Based on 1H and 13C NMR spectroscopy data, it was found that the main monomeric phenolic extractive substances of cedar wood are stilbene – pinosilvin monomethyl ether (content in cedar wood 0.25% of a.s.) and flavonoids – tectochrizin, pinocembrin, and pinobankxin, as well as oligomeric and polymeric phenolic compounds. It was shown that the composition of cedar wood oligomers includes a complex mixture of stilbene derivatives of pinosilvin and flavonoids with the inclusion of carbohydrate residues. The content of the fraction of oligomeric and polymeric phenolic compounds in cedar wood is 0.39% of a.s.

Sign in / Sign up

Export Citation Format

Share Document