scholarly journals COMPARATIVE STUDY OF ALCOHOL EXTRACTS OF PARTICLES OF CALENDULA OFFICINALIS L. DECORA-TIVE NEON MARK BY GAS CHROMATOGRAPHY/MASS-SPECTROMETRY

2020 ◽  
pp. 207-213
Author(s):  
Marina Gennad'yevna Pervova ◽  
Artem Andreyevich Burgart ◽  
Mariya Andreyevna Samorukova ◽  
Tat'yana Viktorovna Shatunova ◽  
Marina Vadimovna Semenovykh ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Component Composition ◽  
Ethyl Esters ◽  
Biologically Active ◽  
Gas Chromatography Mass Spectrometry ◽  
Mass Yield ◽  
Calendula Officinalis ◽  
Chromatography Mass Spectrometry ◽  
Calendula Officinalis L

The paper presents the results of a comparative analysis of the component composition of alcoholic extracts of particles of Calendula officinalis L. decorative Neon mark: stalks, roots, leaves, flowers, etc., by gas chromatography/mass spectrometry. As a result of the study, more than 30 biologically active compounds (BAС) were identified that belong to different classes of organic compounds (including sesquiterpens, hydrocarbons, fatty acids and their ethyl esters, phytosterols). Their relative quantitative assessment was calculated by the method of internal normalization. It was found that the mass yield of extractable substances depends on the part of the plant used: the maximum (about 10%) from the petals and flowers, the minimum (less than 1%) from the roots. It has been established that the detected BAC is contained in all parts of the plant, but their content varies greatly: the most enriched are petals and flowers, the least - the stems, leaves, roots. Vitamin E is found only in flowers, flower petals and calyxes. The presence of a large number of phytosteros in Calendula officinalis L. is of interest for further pharmacognostic studies of this plant.

scholarly journals Identification of O-acetyl-5-methoxytryptophenol in the pineal gland by gas chromatography-mass spectrometry

1980 ◽  
Vol 185 (2) ◽  
pp. 537-540 ◽  
Author(s):  
I Smith ◽  
P Francis ◽  
R M Leone ◽  
P E Mullen
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Pineal Gland ◽  
Biologically Active ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Active Precursor

The presence of O-acetyl-5-methoxytryptophol in the pineal glands of rats kept in the dark for 8 h, but not in the light, has been shown by means of g.l.c.-mass spectrometry. It is suggested that this compound may be the biologically active precursor of circulating methoxytryptophol.

scholarly journals Phytochemical screening and GC–MS analysis of bioactive compounds present in ethanolic leaves extract of Silybum marianum (L).

2019 ◽  
Vol 9 (1) ◽  
pp. 85-89 ◽  
Author(s):  
M Padma ◽  
S Ganesan ◽  
T Jayaseelan ◽  
S Azhagumadhavan ◽  
P Sasikala ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Bioactive Compounds ◽  
Ethanolic Extract ◽  
Biologically Active ◽  
Gas Chromatography Mass Spectrometry ◽  
Silybum Marianum ◽  
Selective Sequential Extraction ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis

Objective: To investigate the phytochemicals and GC-MS analysis of ethanol extracts of Silybum marianum. Methods: The air-dried leaves were powdered and subjected to selective sequential extraction using solvents of increasing polarity through percolation, ethanol to obtain an ethanolic extract. Then, each of the extracts was further subjected to gas chromatography-mass spectrometry. Results: Qualitative determination of the different biologically active compounds from crude extracts of Silybum marianum using gas chromatography-mass spectrometry revealed different types of high and low molecular weight chemical entities with varying amounts present in each of the extracts. These chemical compounds are considered biologically and pharmacologically important. Conclusions: The study established the chemical composition and anticancer activity of the plant. Keywords: Silybum marianum, Phytochemicals screening, GC–MS analysis, Bioactive compounds.

scholarly journals Complex biochemical characteristics of broccoli and cauliflower

2020 ◽  
pp. 104-111
Author(s):  
D. A. Fateev ◽  
A. E. Solovyeva ◽  
T. V. Shelenga ◽  
A. M. Artemyeva
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Brassica Oleracea ◽  
Biochemical Composition ◽  
Quality Characteristics ◽  
Biologically Active ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Biochemical Quality ◽  
Brassica Oleracea L

Relevance. A commonly cultivated and demanded type of vegetable crops – cabbage Brassica oleracea L. – in the process of evolution and domestication was divided into three clusters: leafy cabbage, headed cabbage and broccoli (cauliflower). According to modern data, Broccoli was developed by hybridization from collard greens and is the genetic precursor to cauliflower. Broccoli and cauliflower have a valuable biochemical composition and are recommended for daily consumption. The characteristic of cultures of Brassica oleracea L. (broccoli and cauliflower) is given according to the most important biochemical quality characteristics. Material and methods. The research material included 30 accessions of broccoli and 35 accessions of cauliflower from the VIR collection, reflecting the ecological, geographical and genetic diversity. Accessions were grown at the research and production base "Pushkin and Pavlovsk Laboratories VIR" (St. Petersburg), biochemical analysis was carried out in the laboratory of biochemistry and molecular biology VIR using gas chromatography/mass spectrometry. Results. The characterization of Brassica oleracea L. species (broccoli and cauliflower) is given according to the main most important biochemical quality characteristics. As a result of using of the modern gas chromatography/mass spectrometry approach to the study of the biochemical composition in samples of broccoli and cauliflower, about 136 components have been identified from the groups of organic acids, free amino acids, including essential, fatty acids, including essential, polyhydric alcohols, sugars, and also phenolic compounds, nucleosides, and others. Regularity in the accumulation of nutrient and biologically active substances by cultures of Brassica oleracea L., by cultivar types and individual samples were revealed. As a result of our research using modern techniques new data on the biochemical composition of broccoli and cauliflower were obtained. B.oleracea within the studied botanical varieties and cultivar types has a complex biochemical composition that characterizes them as accessions with potentially high value, which confirms the need for indepth control of the biochemical composition of plants when breeding new varieties. Accessions with the optimal component composition for balanced human nutrition, which are proposed to be used in breeding for quality, including obtaining varieties for healthy (functional) and therapeutic and prophylactic nutrition of the population of the Russian Federation were found.

scholarly journals ANALISIS FATTY ACID ETHYL ESTER PADA RAMBUT MENGGUNAKAN GAS CHROMATOGRAPHY- MASS SPECTROMETRY UNTUK MENDIAGNOSIS PENYALAHGUNAAN ETANOL

Jurnal Kimia ◽  
2020 ◽  
pp. 175
Author(s):  
N. M. Suaniti ◽  
I W. Wiratama ◽  
I. E. Suprihatin
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Fatty Acid ◽  
Ethyl Ester ◽  
Acid Ethyl Ester ◽  
Fatty Acid Ethyl Ester ◽  
Fatty Acid Ethyl Esters ◽  
Ethyl Esters ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

Fatty acid ethyl ester (FAEE) merupakan salah satu biomarker konsumsi etanol. Penelitian ini bertujuan untuk menganalisis senyawa FAEE dalam sampel rambut secara kualitatif dengan GC-MS. Pada penelitian ini dibuat campuran standar FAEE (Etil Pamlitat dan Etil Oleat) 100 ppm dengan perbandingan 1:1 kemudian dibuat variasi konsentrasi. Metode validasi standar FAEE diukur menggunakan GC-MS. Sampel rambut dipreparasi dengan cara didekontaminasi kemudian diekstraksi dan diinjeksikan ke sistem GC-MS. Hasil validasi diperoleh persamaan regresi linier yaitu, y = 240713,507x-147471,2353 untuk etil palmitat dan y = 58361,45x+242,5 untuk etil oleat dengan nilai koefisien korelasi (r2) berturut-turut sebesar 0,9996 dan 0,9999. Batas deteksi diperoleh sebesar 2,5522 ppm dan 0,0189 ppm, dengan batas kuantitasi sebesar 8,5073 ppm dan 0,0630 ppm. Nilai koefisien variansi diperoleh sebesar 3,59% dan 8,65 x 10-4%, dengan persen perolehan kembali sebesar 100,05% dan 100,08%. Hasil analisis menunjukkan, sampel rambut semua sukarelawan mengandung etil palmitat dan etil oleat pada waktu retensi 22,03 menit dan 23,83 menit. Kata kunci: Fatty acid ethyl esters, Etil Palmitat, Etil Oleat, Alkohol, Etanol, Biomarker, GC-MS

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