scholarly journals Spore morphology of some species of the genus Сystopteris Bernh. from the territory of Altai-Sayan and Trans-Ili Alatau

2020 ◽  
Vol 19 (2) ◽  
pp. 102-107
Author(s):  
A. A. Batkin ◽  
A. V. Vaganov ◽  
A. A. Kechaykin ◽  
A. I. Shmakov
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Small Difference ◽  
Minimum Size ◽  
Individual Species ◽  
Spore Morphology ◽  
State University ◽  
Sayan Region ◽  
Size Ofthe ◽  
Scanning Electron

The spores of the following species from genus Cystopteris Bernh from the Trans-Ili Alatau and AltaiSayan regions were studied by scanning electron microscopy (SEM): Cystopteris almaatensis Kotukhov, Cystopterisdickieana R. Sim, Cystopteris gureevi Stepanov, Cystopteris fragilis Bernh. The characters of their perisporium wererevealed. The results obtained from the territories of the Altai-Sayan region and the Trans-Ili Alatau confirm the divisionof the spores by the type of perisporium published previously by the scientists of Tomsk State University. Thus, weobserved differences in the number and size of the elements of the peresporium, as well as a small difference in the size ofthe spores of individual species. Most spores are characterized by the same minimum size values, but differ in maximumsize. The data on the quantity of elements of perisporium also varies, for example, spores from the Altai-Sayan region andthe Trans-ili Alatau are characterized by their smaller number.

Taxonomic application of macro and micro morphological characters of seeds in Astragalus L. (Galegeae, Fabaceae) in India

Phytotaxa ◽  
2021 ◽  
Vol 502 (2) ◽  
pp. 191-207
Author(s):  
SHIVANI KASHYAP ◽  
CHANDAN KUMAR SAHU ◽  
ROHIT KUMAR VERMA ◽  
LAL BABU CHAUDHARY
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Seed Coat ◽  
Morphological Plasticity ◽  
Morphological Characters ◽  
Individual Species ◽  
Large Size ◽  
The Individual ◽  
Scanning Electron

Due to large size and enormous morphological plasticity, the taxonomy of the genus Astragalus is very complex and challenging. The identification and grouping of species chiefly based on macromorphological characters become sometimes difficult in the genus. In the present study, the micromorphology of the seeds of 30 species belonging to 14 sections of Astragalus from India has been examined applying scanning electron microscopy (SEM) along with light microscopy (LM) to evaluate their role in identification and classification. Attention was paid to colour, shape, size and surface of seeds. The overall size of the seeds ranges from 1.5–3.2 × 0.8–2.2 mm. The shape of the seeds is cordiform, deltoid, mitiform, orbicular, ovoid and reniform. The colour of seeds varies from brown to blackish-brown to black. Papillose, reticulate, ribbed, rugulate and stellate patterns were observed on the seed coat surface (spermoderm) among different species. The study reveals that the seed coat ornamentations have evolved differently among species and do not support the subgeneric and sectional divisions of the genus. However, they add an additional feature to the individual species, which may help in identification in combination with other macro-morphological features.

A Model Outreach Program for Teaching Scanning Electron Microscopy Technology: Successes and Failures

1998 ◽  
Vol 4 (S2) ◽  
pp. 40-41
Author(s):  
N.R. Smith ◽  
R.A. Quinta
Keyword(s):  
Electron Microscopy ◽  
Community College ◽  
Scanning Electron Microscopy ◽  
Outreach Program ◽  
Specimen Preparation ◽  
State University ◽  
Learning Module ◽  
Imaging Center ◽  
Effective Use ◽  
Scanning Electron

A partnership has developed between the Microscope and Graphic Imaging Center (MAGIC) at California State University, Hayward and Ohlone Community College. The purpose of the collaboration is to develop a program to allow community college students to gain experience in preparing and viewing samples using scanning electron microscopy technology. The learning module involves students from the Ohlone College Biology Majors Program and student mentors from CSUH. An additional component is the introduction of under-represented students into a Biology Fellowship Program in which they also participate in the SEM learning module. Participants for these programs are selected on the basis of their interest and how this experience will benefit them as expressed in a one-page written essay. Ten students are selected to participate in the programs.The objectives of the learning module are to: 1) learn specimen preparation techniques and develop skills in SEM technology; 2) gain hands-on experience and develop some laboratory skills necessary for effective use of a SEM in studying biological specimens; 3) share the experience gained with peers at their home institution.

scholarly journals Natural infection by Procyrnea uncinipenis (Nematoda, Habronematidae), a parasite from rheas, an autoctone bird from South America, in emus Dromaius novaehollandiae, a ratite from New Zealand

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Nicole Brand Ederli ◽  
Samira Salim Mello Gallo ◽  
Francisco Carlos Rodrigues de Oliveira
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
New Zealand ◽  
Gastrointestinal Tract ◽  
Natural Infection ◽  
State University ◽  
Dromaius Novaehollandiae ◽  
The City ◽  
Janeiro State ◽  
Scanning Electron

Abstract The present study reports a natural infection of emus, Dromaius novaehollandiae, by the nematode Procyrnea uncinipenis. Five adult emus from a scientific breeding farm at North Fluminense State University located in the city of Campos dos Goytacazes, Rio de Janeiro state, Brazil were necropsied, and their gastrointestinal tract were collected and examined for the presence of parasites from October 2013 to November 2015. Two of the five (40%) emus necropsied were infected with nematodes, and a portion of the nematodes were processed for light microscopy. In addition, two other nematodes (a male and a female) were prepared for scanning electron microscopy. In a female bird, one nematode was collected in the proventriculus and two nematodes in the gizzard and in the male bird four nematodes were collected in the gizzard. The morphological and morphometric analyzes allowed to identify the nematodes as P. uncinipenis, this being the first report of an infection by P. uncinipenis in emus. Therefore, we infer that these emus were naturally infected by nematodes that were considered specific to rheas.

Spore morphology in Coleosporium plumeriae

1980 ◽  
Vol 58 (23) ◽  
pp. 2454-2458 ◽  
Author(s):  
J. A. Traquair ◽  
E. G. Kokko
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Rust Fungus ◽  
Spore Morphology ◽  
Scanning Electron

Coleosporium plumeriae Pat. is a rust fungus (Melampsoraceae) found on Plumeria species. The spermagonial and aecial states are unknown. Light and scanning electron microscopy of uredinia have revealed catenulate, verrucose urediniospores with scattered germ pores. We expect that, as in other Coleosporium species, the urediniospores are morphologically similar to aeciospores. The urediniospore ornamentation consists of distinctive, annulate, bluntly capitate tubercles. Light microscopy of telia indicates that teliospores are smooth, gelatinous, and one celled, quickly appearing four celled with division of the protoplast into an internal four-celled basidium.

scholarly journals INVENTARISASI DAN KAJIAN PALINOLOGI JENIS-JENIS TUMBUHAN PAKU (PTERODOFITA) EPIFIT DI KAWASAN UNIVERSITAS RIAU, PROVINSI RIAU

2019 ◽  
Vol 19 (2) ◽  
pp. 214
Author(s):  
Nery Sofiyanti ◽  
Putri Handayani Harahap
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Digital Data ◽  
Spore Morphology ◽  
Spore Type ◽  
Mature Leaves ◽  
Digital Microscope ◽  
Fern Species ◽  
Surface Ornamentation ◽  
Scanning Electron

Abstrak : Tumbuhan paku  (Pteridofita) epifit banyak di jumpai di kawasan Universitas Riau. Karakteristik spora pada tumbuhn apaku memegang peranan penting dalam kajian taksonomi. Penelitian ini bertujuan untuk mengidentifikasi jenis-jenis pteridofita epifit di kawasan ini dan  mengkarakterisasi sporanya. Metode pengambilan sampel dilakukan menggunakan metode eksplorasi. Setiap jenis yang dijumpai didokumentasikan, dibuat herbarium, dideskripsi dan diidentifikasi. Spora dikoleksi dari daun yang sudah dewasa dan dibuat preparat menggunakan metode asetolisis. Preparat spora diamati dan didokumentasikan menggunakan mikroskop digital. Data yang diperoleh kemudian disajikan dalam bentuk gambar dan tabel serta dianalisis secara deskriptif. Hasil inventarisasi paku epifit di kawasan Universitas Riau mengidentifikasi 18 jenis paku epifit, yang tergolong ke dalam 6 famili yaitu Aspleniaceae, Davalliaceae, Nephrolepidaceae, Polypodiaceae Pteridaceae and Thelypteridaceae. Namun kajian palinologi hanya dilakukan pada 11 jenis yang sudah menghasilkan spora. Hasil pengamatan spora menunjukan bahwa semua jenis paku epifit mempunyai tipe dasar spora monolete, berbentuk ginjal dan hanya mempunyai satu laesura pada bagian ventral. Ukuran spora yang dijumpai adalah besar dan sangat besar, dengan ornamentasi permukaan Lohpat, verukat berpapila verukat, tuberkulat, ekinat pendek dan ekinat panjang. Morfologi spora yang ditemukan pada penelitian ini menunjukan karakteristik yang berbeda pada setiap jenis. Namun masih perlu dilanjutkan pengamatan menggunakan Scanning Electron Microscopy untuk mendapatkan oramentasi lebih detilKata kunci : paku epifit, palinologi, spora, monolete, UNRI Abstract : Ephypitic ferns are commonly found in University of Riau area. Spore characteristics play important role in taxonomical words. This study aimed to identify ephypitic pteridophyte species from this area and characterize their spore. Samples were collected using exploration method, and were then documented, prepared for herbarium, described and identified. Spore grains were collected from mature leaves and prepared by using acetolysis method. The spores were then observed and documented using digital microscope. Data were presented in figures and tables and describtively analized. The inventory of ephypitic ferns from University of Riau area identified a total of 18 fern species belong to 6 families, i.e. Aspleniaceae, Davalliaceae, Nephrolepidaceae, Polypodiaceae, Pteridaceae and Thelypteridaceae. Palinologycal study had been carried out from 11 species that produced spore. We observed the basic spore type of examined ephypitic ferns, monolete, with reniform shape and one laesura at the ventral part. The size of spore observed were big and very big spore, with surface ornamentation Lohpate, papillous verucate,  verucate,  tuberculate,, short echinate and long echinate. Spore morphology observed in this study showed the characteristic among the examined species. The further study using Scanning Electron Microscopy is neccesary to obtain detail spore ornamentation.Keywords: ephypitic fern, palynology, spore, monolete, UNRI

scholarly journals SPORE MORPHOLOGY OF SOME BRYOPHYTA IN EGYPT

2021 ◽  
Vol 21 (no 1) ◽  
Author(s):  
Gamal M. A. Lashin ◽  
Usama Y. Abo-Salama ◽  
Ekram M. Abd El Haliem ◽  
Gehad A. A. Hamouda ◽  
Gehad A. A. Hamouda ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Polar Axis ◽  
Spore Morphology ◽  
Funaria Hygrometrica ◽  
Spore Ornamentation ◽  
Equatorial Diameter ◽  
Spore Ultrastructure ◽  
Scanning Electron

In this work, spore morphology of eight species, Tortula muralis, Tortula brevissima, Aloina brevirostris, Syntrichia leavipila, Microbryum clavallianum (Pottiaceae); Funaria hygrometrica, Entosthodon muhlenbergii, Entosthodon attenuates (Funariaceae); were examined by Light microscopy (LM) and Scanning electron microscopy (SEM). All spores are small; the length of polar axis is between 7.5 ?m and 18.5 ?m, equatorial diameter is between 10.5 ?m and 27 ?m. The smallest spores of them are Tortula brevissima and the biggest spores of them are Entosthodon attenuates. The shapes of the spores are determined as suboblate for Tortula muralis, Aloina brevirostris, Syntrichia leavipila, and oblate for Tortula brevissima, Funaria muhlenbergii, Funaria hygrometrica, Entosthodon attenuates. The ornamentation observed can be regulate, verrucate- regulate, baculate, verrucate, clavate- foveolate, clavate, foveolate- psilate on the distal pole, spore ornamentation of the proximal face is different or less complex than the distal face. We can concluded that the spore ultrastructure give important role for identification and taxonomic significant.

Application of Scanning Electron Microscopy to Myxomycete Taxonomy

1970 ◽  
Vol 28 ◽  
pp. 274-275
Author(s):  
Judith A. Murphy
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscope ◽  
Morphological Characteristics ◽  
Morphological Differentiation ◽  
Initial Study ◽  
Spore Morphology ◽  
Surface Scanning ◽  
Fruiting Stage ◽  
Scanning Electron

Taxonomy of the Myxomyoetes has been based primarily on morphological characteristics observed in the light microscope. Although the fruiting stage is sensitive to environmental conditions, most species exhibit a distinctive although variable category, and thus characteristics of this stage have been used for morphological differentiation. There has been and still is, however, much disagreement concerning the classification and relationships between members of this group. Although capillitial and spore morphology are used in taxonomic classifications, because of their small dimensions they are often poorly defined or not visible in the light microscope. These characteristics are easily discernible by surface scanning electron microscopy (SEM). The purpose of this initial study was to investigate appropriate sample preparations and determine if taxonomically valuable information could be provided with the use of SEM.

Minimum-size detection limit for volcanic glass particles using automated scanning electron microscopy

1992 ◽  
Vol 50 (2) ◽  
pp. 1486-1487
Author(s):  
Mark S. Germani
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Ice Core ◽  
Ice Cores ◽  
Volcanic Glass ◽  
Volcanic Eruptions ◽  
Minimum Size ◽  
Climatic Impact ◽  
Automated Scanning ◽  
Scanning Electron

Ice cores contain a detailed record of fallout from large volcanic eruptions. Identification of volcanic glass particles is used to aid in dating ice cores (tephrachronology). In addition, it should be possible to relate concentrations of volcanogenically-derived species; silicate glass particles, sulfate (from oxidation of SO2), chloride and fluoride to atmospheric levels which existed shortly after eruption. This information, coupled with proxy meteorological records from the core, can be used to assess the climatic impact from major volcanic eruptions.Automated scanning electron microscopy has been used to detect volcanic glass particles >1 μm in diameter in ice core meltwater samples filtered onto Nuclepore filters. It is important to be able to detect submicrometer volcanic glass particles because of their longer atmospheric residence time and the fact that they comprise a significant portion of the number of glass particles deposited in an ice core. Existing procedures for automated analysis of micrometer size particles need to be modified to efficiently analyze submicrometer particles.

scholarly journals The inventory and spore morphology of ferns from Bengkalis Island, Riau Province, Indonesia

2019 ◽  
Vol 20 (11) ◽  
Author(s):  
Nery Sofiyanti ◽  
Mayta Novaliza Isda ◽  
ERWINA JULIANTARI ◽  
RISSAN SURIATNO ◽  
SYAFRONI PRANATA
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Coastal Region ◽  
Spore Morphology ◽  
Main Character ◽  
On This Island ◽  
Fern Species ◽  
Study Sites ◽  
Scanning Electron

Abstract. Sofiyanti N, Isda MN, Juliantari E, Pranata S, Suriatno R. 2019. The inventory and spore morphology of ferns from Bengkalis Island, Riau Province, Indonesia. Biodiversitas 20: 3223-3236. Bengkalis Island is one of main islands at coastal region of Riau Province, Indonesia. The first fern inventory had been conducted on this island, to identify the fern checklist as well as examined the morphology of their spores. Samples were collected from 2 subdistricts and 12 study sites, using exploration method. The spore specimens were coated using AU, before observation using Scanning Electron Microscopy (SEM). A total of 22 fern species are recorded from Bengkalis Islands. These species belong to 3 orders, i.e. Gleicheniales (1 species), Polypodiales (20 species) and Schizaeales (1 species). The spore characteristic indicated similar unity of spore, i.e. monad spore, with triangular, subtriangular, tetrahedral and reniform shape. Three basic types of spore have been observed, i.e. monolete, dilete, and trilete. The spore class observed in this study are small (22 ± 0.88 µm), medium (27.35 ± 1.45 to 47.85 ± 0.88 µm) and large (51.34 ± 1.83 to 53.8 ± 1.81 µm). The ornamentation of perisphore is the main character to distinguished species within one genus.

ANALYSIS OF PRECIOUS GEMSTONES IN THE COLLECTION OF THE EARTH SCIENCE MUSEUM, BY OPTICAL AND SCANNINGELECTRON MICROSCOPY. THE FIRST RESULTS

2021 ◽  
Vol 43 (3) ◽  
pp. 361-367
Author(s):  
Natalya GROMALOVA ◽  
◽  
Peter CHEKOVICH ◽  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Earth Science ◽  
Science Museum ◽  
State University ◽  
The Earth ◽  
First Results ◽  
Diamond Single Crystals ◽  
Microscopy Data ◽  
Scanning Electron

For the first time, a study of the chemical composition and diagnostic properties of gemstones of the main collection of the Earth Science Museum at Moscow State University has been carried out. The modern non-destructive analytical methods were used. These were the optical and scanning electron microscopy, which make it possible to study material composition without special sampling or probing, the latter fact being highly relevant in the museum field. Optical microscopy data showed that the majority of the studied gems contain a lot of fluid and solid inclusions of other minerals, indicating the natural origin of the examined samples. Typomorphic features of emerald crystals show a shale genetic type of this species. Data from scanning electron microscopy revealed the presence of boron impurity in the composition of diamond single crystals.

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