scholarly journals The inventory and spore morphology of ferns from Bengkalis Island, Riau Province, Indonesia

2019 ◽  
Vol 20 (11) ◽  
Author(s):  
Nery Sofiyanti ◽  
Mayta Novaliza Isda ◽  
ERWINA JULIANTARI ◽  
RISSAN SURIATNO ◽  
SYAFRONI PRANATA
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Coastal Region ◽  
Spore Morphology ◽  
Main Character ◽  
On This Island ◽  
Fern Species ◽  
Study Sites ◽  
Scanning Electron

Abstract. Sofiyanti N, Isda MN, Juliantari E, Pranata S, Suriatno R. 2019. The inventory and spore morphology of ferns from Bengkalis Island, Riau Province, Indonesia. Biodiversitas 20: 3223-3236. Bengkalis Island is one of main islands at coastal region of Riau Province, Indonesia. The first fern inventory had been conducted on this island, to identify the fern checklist as well as examined the morphology of their spores. Samples were collected from 2 subdistricts and 12 study sites, using exploration method. The spore specimens were coated using AU, before observation using Scanning Electron Microscopy (SEM). A total of 22 fern species are recorded from Bengkalis Islands. These species belong to 3 orders, i.e. Gleicheniales (1 species), Polypodiales (20 species) and Schizaeales (1 species). The spore characteristic indicated similar unity of spore, i.e. monad spore, with triangular, subtriangular, tetrahedral and reniform shape. Three basic types of spore have been observed, i.e. monolete, dilete, and trilete. The spore class observed in this study are small (22 ± 0.88 µm), medium (27.35 ± 1.45 to 47.85 ± 0.88 µm) and large (51.34 ± 1.83 to 53.8 ± 1.81 µm). The ornamentation of perisphore is the main character to distinguished species within one genus.

scholarly journals INVENTARISASI DAN KAJIAN PALINOLOGI JENIS-JENIS TUMBUHAN PAKU (PTERODOFITA) EPIFIT DI KAWASAN UNIVERSITAS RIAU, PROVINSI RIAU

2019 ◽  
Vol 19 (2) ◽  
pp. 214
Author(s):  
Nery Sofiyanti ◽  
Putri Handayani Harahap
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Digital Data ◽  
Spore Morphology ◽  
Spore Type ◽  
Mature Leaves ◽  
Digital Microscope ◽  
Fern Species ◽  
Surface Ornamentation ◽  
Scanning Electron

Abstrak : Tumbuhan paku  (Pteridofita) epifit banyak di jumpai di kawasan Universitas Riau. Karakteristik spora pada tumbuhn apaku memegang peranan penting dalam kajian taksonomi. Penelitian ini bertujuan untuk mengidentifikasi jenis-jenis pteridofita epifit di kawasan ini dan  mengkarakterisasi sporanya. Metode pengambilan sampel dilakukan menggunakan metode eksplorasi. Setiap jenis yang dijumpai didokumentasikan, dibuat herbarium, dideskripsi dan diidentifikasi. Spora dikoleksi dari daun yang sudah dewasa dan dibuat preparat menggunakan metode asetolisis. Preparat spora diamati dan didokumentasikan menggunakan mikroskop digital. Data yang diperoleh kemudian disajikan dalam bentuk gambar dan tabel serta dianalisis secara deskriptif. Hasil inventarisasi paku epifit di kawasan Universitas Riau mengidentifikasi 18 jenis paku epifit, yang tergolong ke dalam 6 famili yaitu Aspleniaceae, Davalliaceae, Nephrolepidaceae, Polypodiaceae Pteridaceae and Thelypteridaceae. Namun kajian palinologi hanya dilakukan pada 11 jenis yang sudah menghasilkan spora. Hasil pengamatan spora menunjukan bahwa semua jenis paku epifit mempunyai tipe dasar spora monolete, berbentuk ginjal dan hanya mempunyai satu laesura pada bagian ventral. Ukuran spora yang dijumpai adalah besar dan sangat besar, dengan ornamentasi permukaan Lohpat, verukat berpapila verukat, tuberkulat, ekinat pendek dan ekinat panjang. Morfologi spora yang ditemukan pada penelitian ini menunjukan karakteristik yang berbeda pada setiap jenis. Namun masih perlu dilanjutkan pengamatan menggunakan Scanning Electron Microscopy untuk mendapatkan oramentasi lebih detilKata kunci : paku epifit, palinologi, spora, monolete, UNRI Abstract : Ephypitic ferns are commonly found in University of Riau area. Spore characteristics play important role in taxonomical words. This study aimed to identify ephypitic pteridophyte species from this area and characterize their spore. Samples were collected using exploration method, and were then documented, prepared for herbarium, described and identified. Spore grains were collected from mature leaves and prepared by using acetolysis method. The spores were then observed and documented using digital microscope. Data were presented in figures and tables and describtively analized. The inventory of ephypitic ferns from University of Riau area identified a total of 18 fern species belong to 6 families, i.e. Aspleniaceae, Davalliaceae, Nephrolepidaceae, Polypodiaceae, Pteridaceae and Thelypteridaceae. Palinologycal study had been carried out from 11 species that produced spore. We observed the basic spore type of examined ephypitic ferns, monolete, with reniform shape and one laesura at the ventral part. The size of spore observed were big and very big spore, with surface ornamentation Lohpate, papillous verucate,  verucate,  tuberculate,, short echinate and long echinate. Spore morphology observed in this study showed the characteristic among the examined species. The further study using Scanning Electron Microscopy is neccesary to obtain detail spore ornamentation.Keywords: ephypitic fern, palynology, spore, monolete, UNRI

Spore morphology in Coleosporium plumeriae

1980 ◽  
Vol 58 (23) ◽  
pp. 2454-2458 ◽  
Author(s):  
J. A. Traquair ◽  
E. G. Kokko
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Rust Fungus ◽  
Spore Morphology ◽  
Scanning Electron

Coleosporium plumeriae Pat. is a rust fungus (Melampsoraceae) found on Plumeria species. The spermagonial and aecial states are unknown. Light and scanning electron microscopy of uredinia have revealed catenulate, verrucose urediniospores with scattered germ pores. We expect that, as in other Coleosporium species, the urediniospores are morphologically similar to aeciospores. The urediniospore ornamentation consists of distinctive, annulate, bluntly capitate tubercles. Light microscopy of telia indicates that teliospores are smooth, gelatinous, and one celled, quickly appearing four celled with division of the protoplast into an internal four-celled basidium.

Morphology variation of Lutzomyia cruciata eggs (Diptera: Psychodidae: Phlebotominae) in southern Mexico

Zootaxa ◽  
2017 ◽  
Vol 4258 (5) ◽  
pp. 477 ◽  
Author(s):  
ANA CELIA MONTES DE OCA-AGUILAR ◽  
OSCAR MIKERY-PACHECO ◽  
ALFREDO CASTILLO ◽  
EDUARDO A. REBOLLAR-TÉLLEZ ◽  
PETER M. PIERMARINI ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Wide Distribution ◽  
Sand Fly ◽  
Leishmania Mexicana ◽  
Southern Mexico ◽  
Local Adaptations ◽  
Morphological Differences ◽  
Study Sites ◽  
Scanning Electron

The sand fly Lutzomyia cruciata has been associated with the transmission of Leishmania mexicana to humans in Mexico. This species has a wide distribution in Mexico occupying different microhabitats and environments. In this work comparisons of the egg exochorion of Lu. cruciata from different physiographic areas are presented. Study sites are from different states of southern Mexico. Exochorion analysis was carried out using scanning electron microscopy (SEM). Results show differences in the exochorionic pattern among samples from Veracruz (AVER), Yucatán (HOYU) and Chiapas (TACH). The morphotype “Chiapas” has a polygonal crest pattern, the morphotype “Veracruz” shows parallel and longitudinal crests with some or few connections, and the morphotype “Yucatán” has weak connections between crest ridges. These morphological differences could be the result of local adaptations or evidence of divergence within the nominal unit Lutzomyia cruciata.

scholarly journals Raphidascaris (Ichthyascaris) arii sp. n. (Nematoda: Anisakidae), a new ascaridoid nematode from marine catfishes in the Gulf of Thailand

2011 ◽  
Vol 48 (4) ◽  
pp. 262-267 ◽  
Author(s):  
T. Yooyen ◽  
F. Moravec ◽  
C. Wongsawad
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Coastal Region ◽  
Nematode Species ◽  
Gulf Of Thailand ◽  
Male And Female ◽  
The Gulf Of Thailand ◽  
Tail Tip ◽  
Gravid Females ◽  
Scanning Electron

AbstractA new nematode species, Raphidascaris (Ichthyascaris) arii sp. n. (Anisakidae), is described from male and female specimens found in the intestine of two species of marine siluriform fishes, the spotted catfish Arius maculatus (Thunberg) (Ariidae) (type host) and the striped eel catfish Plotosus lineatus (Thunberg) (Plotosidae) from the coastal region of the Gulf of Thailand, Thailand. Based on light and scanning electron microscopy examinations, the new species differs from other nine representatives of the subgenus Ichthyascaris Wu, 1949 mainly in the length of spicules (210–333 μm), body length of gravid females (10–17 mm), and in the presence of small cuticular spines or protuberances on the tail tip of both sexes and 21–30 pairs of preanal and 8 pairs of postanal papillae in the male. This is the first species of this subgenus reported from fishes of the order Siluriformes and the first species of the subgenus Ichthyascaris Wu, 1949 recorded from the Gulf of Thailand.

scholarly journals SPORE MORPHOLOGY OF SOME BRYOPHYTA IN EGYPT

2021 ◽  
Vol 21 (no 1) ◽  
Author(s):  
Gamal M. A. Lashin ◽  
Usama Y. Abo-Salama ◽  
Ekram M. Abd El Haliem ◽  
Gehad A. A. Hamouda ◽  
Gehad A. A. Hamouda ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Polar Axis ◽  
Spore Morphology ◽  
Funaria Hygrometrica ◽  
Spore Ornamentation ◽  
Equatorial Diameter ◽  
Spore Ultrastructure ◽  
Scanning Electron

In this work, spore morphology of eight species, Tortula muralis, Tortula brevissima, Aloina brevirostris, Syntrichia leavipila, Microbryum clavallianum (Pottiaceae); Funaria hygrometrica, Entosthodon muhlenbergii, Entosthodon attenuates (Funariaceae); were examined by Light microscopy (LM) and Scanning electron microscopy (SEM). All spores are small; the length of polar axis is between 7.5 ?m and 18.5 ?m, equatorial diameter is between 10.5 ?m and 27 ?m. The smallest spores of them are Tortula brevissima and the biggest spores of them are Entosthodon attenuates. The shapes of the spores are determined as suboblate for Tortula muralis, Aloina brevirostris, Syntrichia leavipila, and oblate for Tortula brevissima, Funaria muhlenbergii, Funaria hygrometrica, Entosthodon attenuates. The ornamentation observed can be regulate, verrucate- regulate, baculate, verrucate, clavate- foveolate, clavate, foveolate- psilate on the distal pole, spore ornamentation of the proximal face is different or less complex than the distal face. We can concluded that the spore ultrastructure give important role for identification and taxonomic significant.

Application of Scanning Electron Microscopy to Myxomycete Taxonomy

1970 ◽  
Vol 28 ◽  
pp. 274-275
Author(s):  
Judith A. Murphy
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscope ◽  
Morphological Characteristics ◽  
Morphological Differentiation ◽  
Initial Study ◽  
Spore Morphology ◽  
Surface Scanning ◽  
Fruiting Stage ◽  
Scanning Electron

Taxonomy of the Myxomyoetes has been based primarily on morphological characteristics observed in the light microscope. Although the fruiting stage is sensitive to environmental conditions, most species exhibit a distinctive although variable category, and thus characteristics of this stage have been used for morphological differentiation. There has been and still is, however, much disagreement concerning the classification and relationships between members of this group. Although capillitial and spore morphology are used in taxonomic classifications, because of their small dimensions they are often poorly defined or not visible in the light microscope. These characteristics are easily discernible by surface scanning electron microscopy (SEM). The purpose of this initial study was to investigate appropriate sample preparations and determine if taxonomically valuable information could be provided with the use of SEM.

scholarly journals Spore morphology of some species of the genus Сystopteris Bernh. from the territory of Altai-Sayan and Trans-Ili Alatau

2020 ◽  
Vol 19 (2) ◽  
pp. 102-107
Author(s):  
A. A. Batkin ◽  
A. V. Vaganov ◽  
A. A. Kechaykin ◽  
A. I. Shmakov
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Small Difference ◽  
Minimum Size ◽  
Individual Species ◽  
Spore Morphology ◽  
State University ◽  
Sayan Region ◽  
Size Ofthe ◽  
Scanning Electron

The spores of the following species from genus Cystopteris Bernh from the Trans-Ili Alatau and AltaiSayan regions were studied by scanning electron microscopy (SEM): Cystopteris almaatensis Kotukhov, Cystopterisdickieana R. Sim, Cystopteris gureevi Stepanov, Cystopteris fragilis Bernh. The characters of their perisporium wererevealed. The results obtained from the territories of the Altai-Sayan region and the Trans-Ili Alatau confirm the divisionof the spores by the type of perisporium published previously by the scientists of Tomsk State University. Thus, weobserved differences in the number and size of the elements of the peresporium, as well as a small difference in the size ofthe spores of individual species. Most spores are characterized by the same minimum size values, but differ in maximumsize. The data on the quantity of elements of perisporium also varies, for example, spores from the Altai-Sayan region andthe Trans-ili Alatau are characterized by their smaller number.

Pathogenesis of Human Hair Defects

1974 ◽  
Vol 32 ◽  
pp. 12-13
Author(s):  
P.S. Porter ◽  
T. Aoyagi ◽  
R. Matta
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Hair ◽  
Standard Techniques ◽  
Scanning Electron

Using standard techniques of scanning electron microscopy (SEM), over 1000 human hair defects have been studied. In several of the defects, the pathogenesis of the abnormality has been clarified using these techniques. It is the purpose of this paper to present several distinct morphologic abnormalities of hair and to discuss their pathogenesis as elucidated through techniques of scanning electron microscopy.

Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

1977 ◽  
Vol 35 ◽  
pp. 638-639
Author(s):  
P.J. Dailey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Salivary Glands ◽  
Secretory Vesicles ◽  
The Past ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
Gromphadorhina Portentosa ◽  
Scanning Electron ◽  
Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

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