scholarly journals An Educational Interventional Study on Stem Cell Awareness among Eligible Beneficiaries in Central India

2021 ◽  
Vol 10 (43) ◽  
pp. 3715-3719
Author(s):  
Priyesh Marskole ◽  
Soumitra Sethia ◽  
Rashmi Yadav ◽  
Sachin Parmar ◽  
Leena Parihar
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Health Workers ◽  
Central India ◽  
Interventional Study ◽  
Pluripotent Cells ◽  
Cross Sectional ◽  
Potential Source ◽  
Global Registry ◽  
Source Of Information

BACKGROUND Stem cells are pluripotent cells that continuously divide, have the ability of selfrenewal, and can generate complex tissues and organs. Globally there are almost 20 lakh stem cell centres, whereas in India we don’t have such a good network of stem cell registries. The Indian Council of Medical Research (ICMR) study says that if you have 10 lakh stem registries with you, you will be able to serve at least 40 % of the demand, but when we have 20 lakh of the global registry we are not able to meet the demand because of genetic variation. We wanted to spread awareness on stem cells from various levels like NGO’s, government, health sectors and everybody should take the initiative. The objective of the study was to assess knowledge and awareness of stem cells among eligible beneficiaries (ANC mothers and eligible couples) METHODS A cross-sectional educational interventional study was done in the Gwalior region for 2 months. A total of 100 eligible beneficiaries (ANC mothers and eligible couples) were registered for the study. Collection of questionnaire sheets & sorting out of data for analysis was done. RESULTS After awareness, 86 % of the subjects thought that the regenerated tissues from stem cells were good enough to replace the previous tissues and 91 % of the subjects were willing to donate stem cells after awareness. CONCLUSIONS Doctors and health workers can be a potential source of information for these subjects because of effective convincing strategies. KEY WORDS Stem Cells, Awareness, Umbilical Cord Blood (UCB).

Stress resistant human embryonic stem cells as a potential source for the identification of novel cancer stem cell markers

2010 ◽  
Vol 289 (2) ◽  
pp. 208-216 ◽  
Author(s):  
Shaker A. Mousa ◽  
Thangirala Sudha ◽  
Evgeny Dyskin ◽  
Usawadee Dier ◽  
Christine Gallati ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cells ◽  
Cancer Stem Cell ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Stem Cell Markers ◽  
Cell Markers ◽  
Cancer Stem Cell Markers ◽  
Potential Source

Abstract TP401: Inflated Expectations about Stem Cell Therapy in Patients With Chronic Stroke

Stroke ◽  
2013 ◽  
Vol 44 (suppl_1) ◽  
Author(s):  
Jinho Lee ◽  
Kyu-Yong Lee ◽  
Young-Seo Kim ◽  
Hyun Young Kim ◽  
Hyuk Sung Kwon ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Ischemic Stroke ◽  
Cell Therapy ◽  
Stem Cell Therapy ◽  
Chronic Stroke ◽  
Accurate Information ◽  
Stroke Patients ◽  
Media Channels ◽  
Source Of Information

Introduction: Stem cell therapy (SCT) has been proposed for the treatment of neurological disorders. Although there isinsufficient clinical evidence to support its efficacy, unproven SCTs are being performed worldwide. Hypothesis: In this study, we investigated the perspectives and expectations of chronic ischemic stroke patients and physicians about SCTs. Methods: A total of 250 chronic ischemic stroke patients were interviewed at 4 hospitals. Structured open and closed questions about SCT for chronic stroke were asked by trained interviewers using the conventional in-person method. In addition, 250 stroke-related physicians were randomly interviewed via an e-mail questionnaire. Results: Of the 250 patients (mean 63 years, 70% male), 121 (46%) responded that they wanted to receive SCT in spite of its unknown side effects. Around 60% of the patients anticipated physical, emotional, and psychological improvement after SCT, and 158 (63%) believed that SCT might prevent strokes. However, physicians had much lower expectations about the effectiveness of SCTs, which was not in line with patient expectations. Multivariate analysis revealed that male gender (OR: 2.00, 95% CI: 1.10-3.64), longer disease duration (OR: 1.01, 95% CI:1.00-1.02), higher modified Rankin Scale score (OR: 1.30, 95% CI 1.06-1.60), and familiarity with stem cells (OR: 1.86, 95% CI: 1.10-3.15) were independently associated with wanting SCT. The major source of information about SCT was television (68%), and the most reliable source was physicians (49%). Conclusion: Patients have unfounded expectations that SCT will improve their functioning. Considering our finding that the major source of information on stem cells is media channels but not the physician, to decrease patients’ inappropriate exposure, doctors should make more effort to educate patients using mass media with accurate information.

GENERATION OF RABBIT PLURIPOTENT STEM CELL LINES

2012 ◽  
Vol 24 (1) ◽  
pp. 286
Author(s):  
A. Dinnyes ◽  
M. K. Pirity ◽  
E. Gocza ◽  
P. Osteil ◽  
N. Daniel ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Pluripotent Stem Cells ◽  
Pluripotent Stem Cell ◽  
Pluripotent Cells ◽  
Domestic Species ◽  
Isolation And Characterization ◽  
Induced Pluripotent ◽  
Pluripotency Genes

Pluripotent stem cells have the capacity to divide indefinitely and to differentiate to all the somatic tissues. They can be genetically manipulated in vitro by knocking in and out genes, therefore they serve as an excellent tool for gene-function studies and for the generation of models for human diseases. Since 1981, when the first mouse embryonic stem cell (ESC) line was generated, several attempts have been made to generate pluripotent stem cells from other species as it would help us to understand the differences and similarities of signaling pathways involved in pluripotency and differentiation, and would reveal whether the fundamental mechanism controlling self-renewal of pluripotent cells is conserved among different species. This review gives an overlook of embryonic and induced pluripotent stem cell (iPSCs) research in the rabbit which is one of the most relevant non-rodent species for animal models. To date, several lines of putative ESCs and iPSCs have been described in the rabbit. All expressed stem cell-associated markers and exhibited longevity and pluripotency in vitro, but none have been proven to exhibit full pluripotency in vivo. Moreover, similarly to several domestic species, markers used to characterize the putative ESCs are not fully adequate because studies in domestic species have revealed that they are not specific to the pluripotent inner cell mass. Future validation of rabbit pluripotent stem cells would benefit greatly from a reliable panel of molecular markers specific to pluripotent cells of the developing rabbit embryo. The status of isolation and characterization of the putative pluripotency genes in rabbit will be discussed. Using rabbit specific pluripotency genes we might be able to reprogram somatic cells and generate induced pluripotent stem cells more efficiently thus overcome some of the challenges towards harnessing the potential of this technology. This study was financed by EU FP7 (PartnErS, PIAP-GA-2008-218205; InduHeart, PEOPLE-IRG-2008-234390; InduVir, PEOPLE-IRG-2009-245808; RabPstem, PERG07-GA-2010-268422; PluriSys, HEALTH-2007-B-223485; AniStem, PIAP-GA-2011-286264), NKTH-OTKA-EU-7KP HUMAN-MB08-C-80-205; Plurabbit, OMFB-00130-00131/2010 ANR-NKTH/09-GENM-010-01.

scholarly journals Human amnion-derived mesenchymal stem cells are a potential source for uterine stem cell therapy

2008 ◽  
Vol 41 (5) ◽  
pp. 709-725 ◽  
Author(s):  
K. Han ◽  
J. E. Lee ◽  
S. J. Kwon ◽  
S. Y. Park ◽  
S. H. Shim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Cell Therapy ◽  
Stem Cell Therapy ◽  
Human Amnion ◽  
Potential Source

scholarly journals Awareness, attitude and ethical concern of dental practitioners about stem cells: a cross-sectional study

2021 ◽  
Vol 24 (3) ◽  
Author(s):  
Rayan Bahabri ◽  
Nevine Taymour ◽  
Mawadh Ali ◽  
Ghada Alahmadi ◽  
Ayman Mandorah ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Ethical Issues ◽  
Cross Sectional Study ◽  
Lower Percentage ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Ethical Concerns ◽  
Dental Practitioners ◽  
Dental Curriculum

Objective:     To evaluate the level of awareness and attitude among dental practitioners regarding the use of stem cells in dentistry and to determine their knowledge of ethical concerns related to the recent therapy. Material and Methods: A cross-sectional survey-based study was conducted at Taibah University Dental College and Hospital. Medina and at governmental and private dental clinics at the western region of Saudi Arabia. Responses of dental practitioners who completed the survey were recorded between March 2019 and July 2019 without containing any personal identifiers. Level of awareness and attitude and knowledge about ethical issues in relation to stem cell therapy was established. Results: A total of 214 male and female dental practitioners participated in this study and the majority were registered at the Saudi Commission for Health Specialists 128 (59.8%). Dental consultants reported the highest percentage of awareness about dental stem cells (96%, p= 0.005), whereas general dental practitioners (56.2%, p= 0.005) and specialists (52%, p= 0.005), respectively had a lower percentage. When ethical concerns were determined, dental consultants had the highest percentage of knowledge (56%, p= 0.005), whereas dental practitioners (71.2%, p= 0.005) with < 5 years of experience (69.1, p= 0.002) lacked information about related ethical issues. Conclusion: Ways to increase stem cell awareness among dental practitioners in this study recommended including stem cell topics in the dental curriculum and organizing frequent seminars and conferences on this subject.   Keywords Stem cell; Awareness; Ethical; Dental practitioner.

scholarly journals A STUDY ON IMMUNIZATION OF CHILDREN AT URBAN HEALTH TRAINING CENTRE IN UDAIPUR CITY, RAJASTHAN

2020 ◽  
pp. 1-3
Author(s):  
Anjili Mathur ◽  
Anum Fatima ◽  
Abhinav Vyas ◽  
Anuradha Meena ◽  
Shruti Priyadarshini
Keyword(s):  
Secondary School ◽  
Health Workers ◽  
Cross Sectional Study ◽  
Drop Out ◽  
Cross Sectional ◽  
Training Centre ◽  
Attitudes And Practices ◽  
Drop Out Rate ◽  
The Government ◽  
Source Of Information

Background: Immunization is the most economic and most efficient solution to prevent children from infectious diseases. Despite launch of several programmes by the government, parents attitude and knowledge towards vaccination is what matters most. Objective:(i) To study immunization related details of children in general population of UHTC. (ii) To counsel mothers regarding immunization. Methodology: A questionnaire based cross sectional study was conducted for 2 month duration (August and September 2020) among 100 mothers who visited general outdoor of a UHTC in Udaipur city.Data was entered in MS Excel and analysed through SPSS. Result: Majority of the mothers were of the age group 21-25 years (56%),educated upto secondary school (49%), were housewives / unemployed (46%) . The source of information for the maximum participants were health workers. Majority chose government set ups for vaccination of their children (91%).Among all, 69% were fully immunized,30% were partially immunized and only 1% were not immunized at all. An overall 38% drop-out rate was seen. The most common reason of non-vaccination was found to be fear of side effects among the parents (37%).Conclusion: Active and comprehensive involvement of sociologists, behavioural scientists and health personnel is very important to produce effective changes in the attitudes and practices regarding immunization of children.

scholarly journals mRNP Granule Proteins Fmrp and Dcp1a Differentially Regulate Muscle Stem Cell Quiescence via Reciprocally Regulating mRNP Complexes

2020 ◽  
Author(s):  
Nainita Roy ◽  
Malini Pillai ◽  
Farah Patell-Socha ◽  
Swetha Sundar ◽  
Sravya Ganesh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Stem Cell ◽  
Sectional Area ◽  
Cross Sectional ◽  
Muscle Stem Cell ◽  
Translational Repressor ◽  
Stem Cell Quiescence ◽  
Cell Quiescence ◽  
Granule Proteins

Abstract Background: During skeletal muscle regeneration, satellite stem cells use distinct pathways to repair damaged myofibers or to self-renew by returning to quiescence. Cellular/mitotic quiescence employs mechanisms that promote a poised or primed state, including altered RNA turnover and translational repression. Here, we investigate the role of mRNP granule proteins Fragile X Mental Retardation Protein (Fmrp) and Decapping protein 1a (Dcp1a) in muscle stem cell quiescence and differentiation.Methods: Using isolated single muscle fibers from adult mice, we established differential enrichment of mRNP granule proteins including Fmrp and Dcp1a in muscle stem cells vs. myofibers. We investigated muscle tissue homeostasis in adult Fmr1-/- mice, analyzing myofiber cross-sectional area in vivo and satellite cell proliferation ex vivo. We explored the molecular mechanisms of Dcp1a and Fmrp function in quiescence, proliferation and differentiation in a C2C12 culture model. Here, we used polysome profiling, imaging and RNA/protein expression analysis to establish the abundance and assembly status of mRNP granule proteins in different cellular states, and the phenotype of knockdown cells.Results: Quiescent muscle satellite cells are enriched for puncta containing the translational repressor Fmrp, but not the mRNA decay factor Dcp1a. MuSC isolated from Fmr1-/- mice exhibit defective proliferation and mature myofibers show reduced cross-sectional area, suggesting a role for Fmrp in muscle homeostasis. Expression and organization of Fmrp and Dcp1a varies between different cell states in culture. Consistent with its role as a translational repressor, Fmrp is enriched in non-translating mRNP complexes abundant in quiescent myoblasts; Dcp1a puncta are lost in quiescence, suggesting stabilized and repressed transcripts. The function of each protein differs during proliferation; whereas Fmrp knockdown led to decreased proliferation and lower cyclin expression, Dcp1a knockdown led to increased cell proliferation and higher cyclin expression. However, knockdown of either Fmrp or Dcp1a led to compromised differentiation. We also observed cross-regulation of decay versus storage mRNP granules; knockdown of Fmrp enhances accumulation of Dcp1a puncta, whereas knockdown of Dcp1a leads to increased Fmrp in puncta.Conclusions: Taken together, our results provide evidence that the balance of mRNA turnover versus utilization is specific for distinct cellular states.

scholarly journals The knowledge and attitude of medicine students towards stem cells application and donation in neurologic disorders: A study at Guilan University of Medical Sciences, Iran

2021 ◽  
Vol 20 (3) ◽  
pp. 317-323
Author(s):  
Farnaz Salahizadeh Baghmisheh ◽  
◽  
Amaneh Mohammadi Roushandeh ◽  
Sajjad Rezaei ◽  
Alia Sabari ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Medical Students ◽  
Pearson Correlation ◽  
Cross Sectional Study ◽  
Willingness To Participate ◽  
Medical Sciences ◽  
Cross Sectional ◽  
Neurologic Disorders ◽  
Knowledge And Attitude

Background. Medical students have a significant role in improving new perspective about stem cell application in treatment of incurable diseases; such as degenerative neurologic disorders. Methods. In this descriptive cross-sectional study conducted in 2019-2020, 424 medical students at Guilan University of Medical Sciences responded to a questionnaire. The questionnaire was included the questions about knowledge of medical students about application of stem cells in neurologic disorders and stem cells donation and their attitude as well. Exclusion criteria were reluctance to participate in study and less than 20% response to questionnaire. The convenience sampling method was used. Inclusion criteria were being a medical student and willingness to participate in the project. Data were analyzed using SPSS 20 by Pearson correlation, independent student t, one-way ANOVA, U-Mann-Whitney. Results. 49.5% and 43.2% of participants had very low and low knowledge about using the stem cell. The higher score of knowledge was related to higher education periods. 70.3% of medical students had moderate attitude toward using stem cell. Only 17% of them had known the state agencies and private centers of donation. 70.5% had moderate knowledge and 55.2% had moderate attitude toward the stem cell donation. There was a correlation between the knowledge and attitude toward its donation (p = 0.004, r = 0.14). Knowledge about stem cells donation had correlation with the knowledge about its application (p = 0.001, r = 0.161). Conclusion. Although the majority of medical students have very low knowledge about stem cell application in treatment of neurologic disorders, but their attitude toward using stem cell was moderate.

scholarly journals Identification of Novel miRNAs in Mouse Embryonic Stem Cells, Embryonic Fibroblasts, and Reprogrammed Pluripotent Cells

2019 ◽  
Author(s):  
Botao Zhao ◽  
Chunsun Fan
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cells ◽  
De Novo ◽  
Embryonic Stem ◽  
Induced Pluripotent Stem Cell ◽  
Mouse Embryonic Stem Cells ◽  
Pluripotent Cells ◽  
Embryonic Fibroblasts ◽  
Species Specific

AbstractMicroRNAs (miRNAs) are a class of non-coding small RNAs that function in almost every known cellular activity. MiRNAs play an important role in gene regulation that controls embryonic stem cell (ESC) pluripotency and differentiation, as well as induced pluripotent stem cell (iPSC) reprogramming. In this study, we identified nine novel miRNAs by mining the deep sequencing dataset from mouse embryonic stem cells, mouse embryonic fibroblasts (MEF) and three kinds of reprogrammed pluripotent cells. Most of them are non-conserved but species-specific and cell-specific miRNAs. Eight miRNAs are derived from gene introns, including a “mirtron” miRNA, miR-novel-41. We also showed that miR-novel-27 is a mouse-specific miRNA and the 5′ arm of its precursor hairpin, embedding the mature miR-novel-27, uniquely exists in mouse species but not in any other Placentalia animals. Notably, the 5′ arm of the pre-miR-novel-27 hairpin shows nearly perfect palindrome to the 3′ arm suggesting that it was generated by inverted duplication of the 3′ arm. By this mechanism, the pre-miR-novel-27 hairpin was de novo gained in the mouse genome. This is a new type of de novo miRNA emergence mechanism in animals, which we called “inverted local half hairpin duplication” here. In addition, very limited nucleotide mutants accumulated on the newly emerged 5′ arm since its birth suggesting an especially young evolutionary history of the miR-novel-27 gene.

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