scholarly journals Microscopic analysis of oil palm (Elaeis guineensis) infection by Ganoderma boninense

2017 ◽  
Author(s):  
Emmanuel Gorea
Plant Omics ◽  
2017 ◽  
Vol 10 (05) ◽  
pp. 247-251 ◽  
Author(s):  
Yurnaliza ◽  
◽  
Rizkita Rachmi Esyanti ◽  
Agus Susanto ◽  
I Nyoman Pugeg Aryantha ◽  
...  

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Nurshafika Mohd Sakeh ◽  
Siti Nor Akmar Abdullah ◽  
Mohammad Nazri Abdul Bahari ◽  
Azzreena Mohamad Azzeme ◽  
Noor Azmi Shaharuddin ◽  
...  

Abstract Background Hemibiotrophic pathogen such as the fungal pathogen Ganoderma boninense that is destructive to oil palm, manipulates host defense mechanism by strategically switching from biotrophic to necrotrophic phase. Our previous study revealed two distinguishable expression profiles of oil palm genes that formed the basis in deducing biotrophic phase at early interaction which switched to necrotrophic phase at a later stage of infection. Results The present report is a continuing study from our previous published transcriptomic profiling of oil palm seedlings against G. boninense. We focused on identifying differentially expressed genes (DEGs) encoding transcription factors (TFs) from the same RNA-seq data; resulting in 106 upregulated and 108 downregulated TFs being identified. The DEGs are involved in four established defense-related pathways responsible for cell wall modification, reactive oxygen species (ROS)-mediated signaling, programmed cell death (PCD) and plant innate immunity. We discovered upregulation of JUNGBRUNNEN 1 (EgJUB1) during the fungal biotrophic phase while Ethylene Responsive Factor 113 (EgERF113) demonstrated prominent upregulation when the palm switches to defense against necrotrophic phase. EgJUB1 was shown to have a binding activity to a 19 bp palindromic SNBE1 element, WNNYBTNNNNNNNAMGNHW found in the promoter region of co-expressing EgHSFC-2b. Further in silico analysis of promoter regions revealed co-expression of EgJUB1 with TFs containing SNBE1 element with single nucleotide change at either the 5th or 18th position. Meanwhile, EgERF113 binds to both GCC and DRE/CRT elements promoting plasticity in upregulating the downstream defense-related genes. Both TFs were proven to be nuclear-localized based on subcellular localization experiment using onion epidermal cells. Conclusion Our findings demonstrated unprecedented transcriptional reprogramming of specific TFs potentially to enable regulation of a specific set of genes during different infection phases of this hemibiotrophic fungal pathogen. The results propose the intricacy of oil palm defense response in orchestrating EgJUB1 during biotrophic and EgERF113 during the subsequent transition to the necrotrophic phase. Binding of EgJUB1 to SNBE motif instead of NACBS while EgERF113 to GCC-box and DRE/CRT motifs is unconventional and not normally associated with pathogen infection. Identification of these phase-specific oil palm TFs is important in designing strategies to tackle or attenuate the progress of infection.


Plant Omics ◽  
2017 ◽  
Vol 10 (02) ◽  
pp. 45-52 ◽  
Author(s):  
Nurul Liyana Rozali ◽  
◽  
Mohd Ambar Yarmo ◽  
Abu Seman Idris ◽  
Ahmad Kushairi ◽  
...  

Author(s):  
M Basyuni ◽  
D Afandi ◽  
R Hayati ◽  
Y Bimantara ◽  
D Arifiyanto ◽  
...  

2018 ◽  
Vol 10 (12) ◽  
pp. 4443 ◽  
Author(s):  
Anna Brunerová ◽  
Miroslav Müller ◽  
Vladimír Šleger ◽  
Himsar Ambarita ◽  
Petr Valášek

The oil palm (Elaeis guineensis Jacq.) represents Indonesian major agriculture crop, nevertheless, its cultivation and processing results in an excessive amount of waste biomass, namely, empty fruit bunches (EFB), which is not always properly processed or reused. Therefore, the present investigation was performed to attract wide public interest in proper waste management and reuse of waste biomass. The suitability of such waste biomass for bio-pellet fuel production within its ecological EFB reuse was the subject of investigation. Its fuel parameters, mechanical quality and microscopic analysis represented the set of experimental testing performed within the target purpose. Satisfactory result values were stated within oil palm EFB fuel parameters, namely, moisture content Mc—7.07%, ash content Ac—9.41% and energy potential NCV—15.06 MJ∙kg−1. Mechanical analysis of the produced bio-pellet fuel proved outstanding results: Volume density ρ—1440.01 kg∙m−3 and mechanical durability DU—97.4% and 99.4% (according to ÖNORM M 7135 (2003) and ISO 17831-1 (2015)). Furthermore, results of compressive strength σ proved the requested high level; in simple pressure σp—10.83 MPa and in cleft σc—60.46 N·mm−1. Stereoscopic microscope measurements proved a prevalent proportion of fiber >97% within the feedstock content, and scanning electron microscopy (SEM) of bio-pellet samples diagnosed cracks purely on the outer surface, not within their internal structures, which indicated high quality compacted products. In conclusion, the overall evaluation indicates the production of environmental-friendly high quality bio-pellet fuel, thus, proving the suitability of oil palm EFB for the production of bio-pellet fuel.


Proceedings ◽  
2020 ◽  
Vol 36 (1) ◽  
pp. 56
Author(s):  
Mudge ◽  
Rama ◽  
Pilotti ◽  
Godwin

Oil palm (Elaeis guineensis Jacq.) is a long-term perennial crop of great economic importance to [...]


2012 ◽  
Vol 9 (2) ◽  
pp. 377-386 ◽  
Author(s):  
Sue-Sean Tee ◽  
Yung-Chie Tan ◽  
Faridah Abdullah ◽  
Meilina Ong-Abdullah ◽  
Chai-Ling Ho

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