scholarly journals Nicotine effect on bone remodeling during orthodontic tooth movement: Histological study in rats

2014 ◽  
Vol 19 (2) ◽  
pp. 96-107 ◽  
Author(s):  
Ricardo Lima Shintcovsk ◽  
Luégya Knop ◽  
Orlando Motohiro Tanaka ◽  
Hiroshi Maruo
Keyword(s):  
Bone Remodeling ◽  
Tooth Movement ◽  
Histological Study ◽  
Bone Matrix ◽  
Polarized Light ◽  
Orthodontic Tooth Movement ◽  
Control Group ◽  
Orthodontic Movement ◽  
Significant Difference ◽  
Male Wistar Rats

Introduction: Nicotine is harmful to angiogenesis, osteogenesis and synthesis of collagen. Objective: The aim of this study was to investigate the effect of nicotine on bone remodeling during orthodontic movement in rats. Methods: Eighty male Wistar rats were randomly divided into three groups: Group C (control), group CM (with orthodontic movement) and group NM (nicotine with orthodontic movement) groups. The animals comprising groups C and CM received 0.9% saline solution while group NM received nicotine solution (2 mg/kg). A nickel-titanium closed-coil spring was used to induce tooth movement. The animals were euthanized and tissue specimens were processed histologically. We quantified blood vessels, Howship's lacunae and osteoclast-like cells present in the tension and compression areas of periodontal ligaments. The extent of bone formation was evaluated under polarized light to determine the percentage of immature/mature collagen. Results: We observed lower blood vessel densities in the NM group in comparison to the CM group, three (p < 0.001) and seven (p < 0.05) days after force application. Osteoclast-like cells and Howship's lacunae in the NM group presented lower levels of expression in comparison to the CM group, with significant differences on day 7 (p < 0.05 for both variables) and day 14 (p < 0.05 for osteoclast-like cells and p < 0.01 for Howship's lacunae). The percentage of immature collagen increased in the NM group in comparison to the CM group with a statistically significant difference on day 3 (p < 0.05), day 7 (p < 0.001), day 14 (p < 0.001) and day 21 (p < 0.001). Conclusions: Nicotine affects bone remodeling during orthodontic movement, reducing angiogenesis, osteoclast-like cells and Howship's lacunae, thereby delaying the collagen maturation process in developed bone matrix.

scholarly journals The effect of autologous leukocyte platelet rich fibrin on the rate of orthodontic tooth movement: A prospective randomized clinical trial

2018 ◽  
Vol 12 (03) ◽  
pp. 350-357 ◽  
Author(s):  
Azita Tehranchi ◽  
Hossein Behnia ◽  
Fereydoun Pourdanesh ◽  
Parsa Behnia ◽  
Nelson Pinto ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Tooth Movement ◽  
Random Effect ◽  
Random Effect Model ◽  
Orthodontic Tooth Movement ◽  
Control Group ◽  
Extraction Socket ◽  
Effect Model ◽  
Significant Difference ◽  
And Control

ABSTRACT Objective: The aim of this study was to evaluate the effect of LPRF, placed in extraction sockets, on orthodontic tooth movement (OTM). Materials and Methods: Thirty extraction sockets from eight patients (five males, three females, with a mean age of 17.37 years; range 12–25 years) requiring extraction of first premolars based on their orthodontic treatment plan participated in this split-mouth clinical trial. In one randomly selected quadrant of each jaw, the extraction socket was preserved as the experimental group by immediate placement of LPRF in the extraction socket. The other quadrant served as the control group for secondary healing. Immediately, the teeth adjacent to the defects were pulled together by a NiTi closed-coil spring with constant force. A piece of 0.016 × 0.022-inch stainless steel wire was used as the main arch wire. The amount of OTM was measured on the study casts at eight time points with 2-week intervals for 3 months. Analysis of random effect model was performed for the purpose of comparison between the experimental and control groups. Results: According to the random effect model, a statistically significant difference was found between the experimental and control group in rate of OTM (P = 0.006). Conclusion: According to the results, application of LPRF, as an interdisciplinary approach combining orthodontics and surgery, may accelerate OTM, particularly in extraction cases.

Locally Inhibition of Orthodontic Relapse by Injection of Carbonated Hydroxy Apatite-Advanced Platelet Rich Fibrin in a Rabbit Model

2017 ◽  
Vol 758 ◽  
pp. 255-263 ◽  
Author(s):  
Ananto Ali Alhasyimi ◽  
Pinandi Sri Pudyani ◽  
Widya Asmara ◽  
Ika Dewi Ana
Keyword(s):  
Bone Remodeling ◽  
Alveolar Bone ◽  
Tooth Movement ◽  
Rabbit Model ◽  
Orthodontic Tooth Movement ◽  
Potential Effect ◽  
Control Group ◽  
Hydroxy Apatite ◽  
Carbonated Apatite ◽  
Alveolar Bone Remodeling

Relapse is considered a significant failure after orthodontic treatment. In response to relapse, RANKL expressions will increase, while OPG expressions will decrease. CHA is thought to be one of an ideal candidate for enhancing bone formation. Moreover, a-PRF is a source high levels of growth factors that play a central role in the bone remodeling. This research was intended to investigate the effect of hydrogel CHA-aPRF in preventing relapse. Hydrogel-CHA was initially designed, with its degradation profile and FTIR (Fourie’s Transform Infrared) spectra were investigated as the basis to find out optimum formulation before incorporated with aPRF. Hydrogel-CHA microspheres were prepared in 3 different compositions: those were encoded 30-CHA, 40-CHA, and 50-CHA. After the hydrogel formulation and characterization were completed, 10 mL blood samples were collected, then centrifuged at 1500 rpm for 14 min. At the end of the centrifugation process, the aPRF clot was isolated and then pressed to obtain their releasate. The releasate aPRF was then loaded into the best formulation candidate of hydrogel CHA. The hydrogel incorporated aPRF was then gently injected on the mesial side of incisor gingival sulcus of the rabbit after orthodontic tooth movement. The FTIR analysis showed that carbonated apatite was successfully developed during the fabrication process of hydrogel-CHA microspheres. It was also known that degradation profile of 30-CHA was considered ideal compared to the other compositions. The application of CHA-aPRF (group C) was proven to significantly prevent relapse, indicated by lowest percentage of relapse 21 days after debonding (29.95±3.91%) compared to control group. Furthermore, it has been found that expressions of RANKL were significantly lowest (p<0.05) in group C on day 0, 3, and 7, while OPG expressions showed significantly highest (p<0.05) in group C on day 14 and 21 after debonding. These results indicate that incorporation of hydrogel-CHA has potential effect to enhance alveolar bone remodeling and prevent orthodontic relapse by stimulates OPG expression and suppresses RANKL expression.

scholarly journals Pain Experience with Micro-osteoperforations during Initial Orthodontic Alignment: A Randomized Clinical Trial.

2020 ◽  
Vol 9 (4) ◽  
pp. 309-318
Author(s):  
Azaitun Akma Shahrin ◽  
◽  
Sarah Haniza Abdul Ghani ◽  
Noraina Hafizan Norman ◽  
◽  
...  
Keyword(s):  
Clinical Trial ◽  
Repeated Measures ◽  
Pain Perception ◽  
Tooth Movement ◽  
Intervention Group ◽  
Orthodontic Tooth Movement ◽  
Control Group ◽  
Initial Alignment ◽  
Significant Difference ◽  
Fixed Appliances

Objetive: The objective of this clinical trial was to investigate the perception of pain during initial maxillary alignment with an adjunctive procedure of micro-osteoperforations (MOPs) compared to conventional orthodontics. Material and methods: This study design was a single-centre, two-arm parallel prospective randomised clinical trial. Thirty consecutive adult subjects (25 females and 5 males; mean age ± SD, 22.66 ± 3.27 years) with 5-8mm moderate upper labial segment crowding were randomly allocated using block randomisation into intervention and control group. All subjects had first premolar extractions, bonded conventional fixed appliances and 0.014-inch nickel-titanium archwire was placed for initial alignment. The intervention group received a 3-mm deep MOPs procedure under local anaesthesia using a Propel device (PROPEL Ortho Singapore) on the labiogingival aspect between the maxillary incisors. Both groups received a set of 100 mm visual analogue scale to complete over the first week, recording pain at 24 hours, 3 days and 1 week. Data were analysed using repeated-measures analysis of variance (ANOVA). Results: There was a statistically significant difference observed in perceived pain levels between MOPs and the control group on day 1, day 3 and day 7 postoperatively. Pain perception was significantly lower in the intervention group at all time points. Conclusion: Accelerating orthodontic tooth movement with MOPs did not accentuate pain perceived during initial maxillary alignment with fixed appliances.

scholarly journals Histological changes during orthodontic tooth movement due to hyperbaric oxygen therapy

2017 ◽  
Vol 49 (2) ◽  
pp. 63
Author(s):  
Arya Brahmanta ◽  
Sutjipto Sutjipto ◽  
Ida Bagus Narmada
Keyword(s):  
Bone Remodeling ◽  
Hyperbaric Oxygen ◽  
Hyperbaric Oxygen Therapy ◽  
Periodontal Ligament ◽  
Oxygen Therapy ◽  
Tooth Movement ◽  
Statistical Tests ◽  
Orthodontic Tooth Movement ◽  
Control Group ◽  
Ligament Size

Background: Mechanical force of orthodontics causes changes in periodontal ligament vascularization and blood flow, resulting in biochemical and cellular changes as well as changes in the contour of the alveolar bone and in the thickness of the periodontal ligaments. Hyperbaric oxygen (HBO) therapy is one of many solutions stimulating the growth of new blood vessels and increasing tissue oxygenation. Thus, HBO plays a role in recovery of periodontal ligament and osteoblasts. Purpose: This study aimed to determine the effects of HBO therapy for seven days on periodontal ligament size and osteoblast number in the tension site during bone remodeling in tooth movement. Method: The study was true experimental laboratories with completely randomized control group post test only design. Twenty-four males guinea pigs were randomly divided into three groups. K0 was the control group without any treatment, K1 was the group given a mechanical orthodontic pressure, and K2 was the group treated with the addition of hyperbaric oxygen therapy. The maxillary incisors were moved distally by elastic separator. After HBO therapy on day 7, all of the groups were sacrificed, and then periodontal ligament size and osteoblast number were analyzed by one-way Anova and LSD statistical tests. Result: The results showed significant differences in the size of the periodontal ligament and the number of osteoblasts in the tension site among the groups (p<0.05). Conclusion: HBO therapy at 2.4 ATA for 7 days is effective in recovery of periodontal ligament and increased osteoblast number during bone remodeling in tension area of orthodontic tooth movement.

scholarly journals Effects of salbutamol, montelukast and prednisone on orthodontic tooth movement in rats

2021 ◽  
Vol 24 (2) ◽  
Author(s):  
Victor Chumpitaz-Cerrate ◽  
Lesly Chávez-Rimache ◽  
César Franco-Quino ◽  
Eliberto Ruiz-Ramirez ◽  
Elías Aguirre-Siancas ◽  
...  
Keyword(s):  
Clinical Evaluation ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Study Groups ◽  
Preclinical Study ◽  
Control Group ◽  
Histopathological Analysis ◽  
Significant Difference ◽  
Interincisal Distance

Objective: To evaluate the effect of salbutamol, montelukast, and prednisone on orthodontic tooth movement in rats. Material and Methods: In vivo experimental preclinical study. The sample consisted of 48 rats randomly distributed in four study groups. Group A was given saline solution; to group B, salbutamol 4 mg/Kg; to group C, montelukast 2.5 mg/Kg and to group D, prednisone 2.5 mg/Kg. All were fitted with orthodontic devices and the medications were administered intraperitoneally every 12 hours for 5 days. The clinical evaluation (variation in the interincisal distance) was performed at one, three, five, and seven days and the histopathological analysis (cell count) at five and seven days. Results: In the clinical evaluation of the variation in the interincisal distance, a significant difference was found in all the evaluations (p <0.05). It was found that the salbutamol group presented higher variation values in the interincisal distance on all the days evaluated. In the histopathological analysis at five and seven days, it was found that the osteoblast and osteocyte count was significantly higher in the salbutamol group compared to the other groups (p ><0.05). However, in the subgroup analysis, it was found that there was no significant difference in the osteoblast and osteocyte count between the prednisone, montelukast, and control group (p>0.05). Conclusion: The administration of salbutamol increased the magnitude of orthodontic tooth movement; nonetheless, the administration of montelukast and prednisone did not modify the magnitude of orthodontic tooth movement in rats.   Keywords Albuterol; Montelukast; Prednisone; Rats; Tooth movement.

scholarly journals Effects of 405-, 532-, 650-, and 940-nm wavelengths of low-level laser therapies on orthodontic tooth movement in rats

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Hasibe Baser Keklikci ◽  
Ahmet Yagci ◽  
Arzu Hanim Yay ◽  
Ozge Goktepe
Keyword(s):  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Positive Control ◽  
Control Group ◽  
Bone Area ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser ◽  
Significant Difference ◽  
532 Nm

Abstract Background Investigating the effects of 405-nm, 532-nm, 650-nm, and 950-nm wavelengths of LLLTs (low-level laser therapies) on the orthodontic tooth movement in rats by using histological and immunohistochemical methods. Forty-five Wistar albino rats were randomly divided into 5 groups: control group (positive control: the left maxillary 1st molar side; negative control: the right maxillary 1st molar side), 405 nm LLLT group (Realpoo), 532 nm LLLT group (Realpoo), 650 nm LLLT group (Realpoo), and 940 nm LLLT group (Biolase). The left maxillary 1st molar teeth of all rats were applied mesially 50-g force. Starting from the 1st day, 48 h intervals, LLLT was applied in continuous wave mode and in contact with the tissue. The application area was approximately 1 cm2. The lasers were performed for 3 min on each surface (buccal, palatal, mesial), totally 9 min (total dose 54 J/cm2). The amount of the molar mesialization, the bone area between the roots, PDL (periodontal ligament) measurements, TRAP (tartrate-resistant acid phosphatase), and ALP (alkaline phosphatase) immunoreactivity intensity were calculated. Results The amount of the molar mesialization was significantly higher in the 650 nm LLLT group (mean 0.878 ± 0.201 mm; 95% CI (confidence interval) 0.724 and 1.032) than in the groups of positive control (mean 0.467 ± 0.357 mm; 95% CI 0.192 and 0.741) and 405 nm LLLT (mean 0.644 ± 0.261 mm; 95% CI 0.443 and 0.845) (p < 0.001). There were significant differences in the PDL-mesial (p = 0.042) and PDL-distal (p = 0.007) regions between the groups. The immunoreactivity intensity for TRAP-mesial was significantly higher in the positive control group (mean 109,420.33 ± 8769.17; 95% CI 100,217.65 and 118,623.02) than in the 405 nm (mean 91,678.83 ± 7313.39; 95% CI 84,003.9 and 99,353.77) and the 650 nm LLLT (mean 87,169.17 ± 4934.65; 95% CI 81,990.56 and 92,347.77) groups (p = 0.002). There was no statistically significant difference between the groups on immunoreactivity intensity with ALP staining. Conclusions The results of this study show that LLLT with 650-nm wavelength increases orthodontic tooth movement more than 405-nm, 532-nm, and 940-nm LLLTs. The 940-nm and 650-nm LLLTs also increase the bone area between the roots by more than 405-nm and 532-nm wavelengths.

scholarly journals The effects of binge-pattern alcohol consumption on orthodontic tooth movement

2014 ◽  
Vol 19 (6) ◽  
pp. 93-98 ◽  
Author(s):  
Cristiano Miranda de Araujo ◽  
Aline Cristina Batista Rodrigues Johann ◽  
Elisa Souza Camargo ◽  
Orlando Motohiro Tanaka
Keyword(s):  
Bone Resorption ◽  
Saline Solution ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Control Group ◽  
Tartrate Resistant Acid Phosphatase ◽  
Chi Square ◽  
Significance Level ◽  
Male Wistar Rats ◽  
Chi Square Test

OBJECTIVE: This study aimed to assess tissue changes during orthodontic movement after binge-pattern ethanol 20% exposure.METHODS: Male Wistar rats (n = 54) were divided into two groups. The control group (CG) received 0.9% saline solution, while the experimental group (EG) received 20% ethanol in 0.9% saline solution (3 g/kg/day). On the 30th day, a force of 25 cN was applied with a nickel-titanium closed coil spring to move the maxillary right first molar mesially. The groups were further divided into three subgroups (2, 14 and 28 days). Tartrate-resistant acid phosphatase and picrosirius were used to assess bone resorption and neoformation, respectively. Data were compared by two-way ANOVA, Tukey's HSD, Games-Howell and chi-square test. Significance level was set at 5%.RESULTS: There was a decrease in the number of osteoclasts in EG at day 28. The percentage of collagen showed no interaction between group and time.CONCLUSION: Binge-pattern 20% ethanol promoted less bone resorption at the end of tooth movement, thereby suggesting delay in tooth movement.

scholarly journals Infrared Light-Emitting Diode (LED) Effects on Orthodontic Tooth Movement

2019 ◽  
Vol 30 (4) ◽  
pp. 410-416 ◽  
Author(s):  
Simone Peixe Friedrichsdorf ◽  
Victor Elias Arana-Chavez ◽  
Vivian Bradaschia-Correa ◽  
Paolo Maria Cattaneo ◽  
Gladys Cristina Dominguez
Keyword(s):  
Tooth Movement ◽  
Root Resorption ◽  
Light Emitting Diode ◽  
Histological Study ◽  
Orthodontic Tooth Movement ◽  
Infrared Light ◽  
Tartrate Resistant Acid Phosphatase ◽  
Micro Computed Tomography ◽  
Led Phototherapy ◽  
Significant Difference

Abstract The present study aimed to analyze the effect of LED phototherapy on the presence of hyalinization and root resorption during orthodontic tooth movement (OTM) in rats and to measure the amount of tooth movement. Eighty rats were allocated into two groups: LED and control (CON), where the LED rats were irradiated with infrared LED (850 nm, 30 mW) for 5 min during the first five days of OTM and where controls were not irradiated. Both groups were subdivided into four subgroups (n=10) according to the date of euthanasia (4, 7, 14 and 21 days). Five out of ten LED21 and five of ten CON21 rats were submitted to micro-computed tomography (μCT); μCT scans were taken on days 0, 7, 14 and 21. For histological study, maxillae were processed to light microscopy using Hematoxylin-Eosin (HE) and Tartrate-Resistant Acid Phosphatase (TRAP) histochemistry. The amount of tooth movement did not differ between LED and CON. Hyalinization was observed at the pressure areas in both groups, and it did not show a statistically significant difference between the groups. Root resorption was also observed in both groups after 7 days and it did not represent any differences between the two groups. LED phototherapy was not able to increase the amount of OTM. Similar characteristics of hyalinization and root resorption were observed in both groups.

scholarly journals The effect of red wine extract, resveratrol, on the degree and rate of orthodontic tooth movement in guinea pigs

2015 ◽  
Vol 5 ◽  
pp. 181-189
Author(s):  
Isidro Alex C. Urriquia ◽  
Lotus D. Llavore
Keyword(s):  
Guinea Pigs ◽  
Low Dose ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Control Group ◽  
High Dose ◽  
Male Adult ◽  
Significant Difference ◽  
The Mean ◽  
And Control

ObjectiveAn animal trial, its protocol approved by the Institutional Animal Care and Use Committee of the U.P. National Institutes of Health (IACUC Protocol No. 2010-008), was employed to investigate the effects of resveratrol on the degree and rate of orthodontic tooth movement in guinea pigs.Materials and MethodsEighteen male adult guinea pigs were randomly allocated into 3 groups: low dose, high dose, and control groups. A 0.016″ titanium molybdenum alloy wire formed into a helical torsion spring with a coil, with the loops cemented onto the maxillary incisors of the animals, served as the orthodontic appliance. Daily oral administration of resveratrol was provided to the low dose (0.047 mg/kg) and high dose (0.47 mg/kg) groups, while water was provided to the control group. Measurements were taken everyday at the interproximal area at the level of the incisal edge using a measuring caliper.ResultsThe results of the ANOVA showed no statistically significant differences in the mean measurements of tooth separation among the three groups from day 2 (P=0.966) to day 8 (P=0.056). However, starting from day 9 (P=0.049) until day 18 (P=0.000), there was a significant difference in the mean tooth separation among the test groups.ConclusionUsing the LSD, it was noted that the low dose and the high dose groups have similar degrees of mean tooth separation, with the control group being significantly different from the two.

scholarly journals Effect of Blue-Light-Emitting Diode Exposure on Collagen Density of Periodontal Ligament During Orthodontic Tooth Movement in Rats

2021 ◽  
Vol 12 (6) ◽  
pp. 8231-8240
Keyword(s):  
Blue Light ◽  
Periodontal Ligament ◽  
Wistar Rats ◽  
Tooth Movement ◽  
Light Emitting Diode ◽  
Orthodontic Tooth Movement ◽  
Control Group ◽  
Light Emitting ◽  
Male Wistar Rats ◽  
Collagen Density

Photobiomodulation therapy using Light Emitting Diode (LED) with a certain period and intensity can stimulate tissue repair and accelerate orthodontic tooth movement. During remodeling, collagen on periodontal ligaments was formed on the pressure and tension sides. The aim of this study was to investigate the effect of exposure time and observation day of blue LED on the collagen density of periodontal ligament of the pressure and tension sides of Wistar rats. Forty-eight male Wistar rats aged 3-4 months weighing 300-500 grams were divided into four groups: the control group; the group exposed to LED with λ1000 nm of 490 mW/cm2 for 25, 30, and 35 seconds once a day. In addition, their mandibular inter-incisor was administered with thirty-five grams orthodontic force using an open coil spring. The calculations on the collagen density of pressure and tension sides were carried out on days 0, 3, 7, and 14 using the TinEye application. Statistical analysis test was conducted using two-way ANOVA and LSD post hoc test. The results showed a more significant increase in the collagen density of the pressure and tension sides than that of the control group (p <0.05). The collagen density of pressure and tension sides reach the highest point at 30 seconds exposure followed by 35 and 25 seconds. The collagen density of pressure sides decreased on the 3rd day and increased afterward, and the tension sides continued to increase on the 3rd day afterward. The study showed that blue light LEDs were able to increase collagen density on the pressure and tension sides, with optimal exposure at 30 seconds, and it reached the highest point at day 14.

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